Anti-Inflammatory Activity of Phyllanthus niruri L. Leaf Extract: In Vitro Study on RAW 264.7 Macrophage Cells and In Silico Analysis
Phyllanthus niruri L. is a medicinal plant recognized for its wide range of therapeutic benefits, particularly its anti-inflammatory properties. This research focused on assessing the impact of P. niruri leaf extract on pro-inflammatory cytokines in vitro using RAW 264.7 macrophage cells, analyze i...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Bogor Agricultural University
2024-12-01
|
| Series: | Hayati Journal of Biosciences |
| Online Access: | https://journal.ipb.ac.id/index.php/hayati/article/view/55992 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841554841758859264 |
|---|---|
| author | Ngurah Intan Wiratmini Made Pharmawati Anak Agung Sagung Alit Sukmaningsih Sentot Joko Raharjo Wahyu Widowati |
| author_facet | Ngurah Intan Wiratmini Made Pharmawati Anak Agung Sagung Alit Sukmaningsih Sentot Joko Raharjo Wahyu Widowati |
| author_sort | Ngurah Intan Wiratmini |
| collection | DOAJ |
| description |
Phyllanthus niruri L. is a medicinal plant recognized for its wide range of therapeutic benefits, particularly its anti-inflammatory properties. This research focused on assessing the impact of P. niruri leaf extract on pro-inflammatory cytokines in vitro using RAW 264.7 macrophage cells, analyze its phytochemical composition, and validate its potential anti-inflammatory mechanisms through molecular docking studies. The extract was analyzed for its phytochemical composition using LC-MS, revealing the presence of sesquiterpene glycosides, flavonoid glycosides, and tannins among the identified compounds. Cell viability tests showed that the extract was not toxic to the cells at concentrations up to 50 µg/ml. Treatment with the extract significantly reduced the levels of pro-inflammatory cytokines TNF-α and IL-1β in LPS-induced RAW 264.7 cells, with the most effective concentration being 50 µg/ml. Molecular docking studies further supported the anti-inflammatory potential of the extract by demonstrating its ability to inhibit COX-2 and TNF-alpha. These findings suggest that P. niruri leaf extract has promising anti-inflammatory properties and needs further investigation as a potential treatment for inflammatory diseases. The in silico study provided insights into the molecular interactions between the extract's compounds and inflammatory targets, supporting its anti-inflammatory mechanism of action.
|
| format | Article |
| id | doaj-art-1210e1b03499449d8d62f1f13d784b51 |
| institution | Kabale University |
| issn | 1978-3019 2086-4094 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Bogor Agricultural University |
| record_format | Article |
| series | Hayati Journal of Biosciences |
| spelling | doaj-art-1210e1b03499449d8d62f1f13d784b512025-01-08T09:00:50ZengBogor Agricultural UniversityHayati Journal of Biosciences1978-30192086-40942024-12-0132210.4308/hjb.32.2.287-299Anti-Inflammatory Activity of Phyllanthus niruri L. Leaf Extract: In Vitro Study on RAW 264.7 Macrophage Cells and In Silico AnalysisNgurah Intan Wiratmini0Made Pharmawati1Anak Agung Sagung Alit Sukmaningsih2Sentot Joko Raharjo3Wahyu Widowati4Biology Study Program, Faculty of Mathematics and Natural Sciences, Udayana University, Jimbaran, Kuta Selatan, Badung, Bali, Indonesia Biology Study Program, Faculty of Mathematics and Natural Sciences, Udayana University, Jimbaran, Kuta Selatan, Badung, Bali, Indonesia Biology Study Program, Faculty of Mathematics and Natural Sciences, Udayana University, Jimbaran, Kuta Selatan, Badung, Bali, Indonesia Diploma of Food and Pharmacy Analyst, Polytechnic of Health of Putra Indonesia Malang, Malang, IndonesiaFaculty of Medicine, Maranatha Christian University, Bandung 40164, Indonesia Phyllanthus niruri L. is a medicinal plant recognized for its wide range of therapeutic benefits, particularly its anti-inflammatory properties. This research focused on assessing the impact of P. niruri leaf extract on pro-inflammatory cytokines in vitro using RAW 264.7 macrophage cells, analyze its phytochemical composition, and validate its potential anti-inflammatory mechanisms through molecular docking studies. The extract was analyzed for its phytochemical composition using LC-MS, revealing the presence of sesquiterpene glycosides, flavonoid glycosides, and tannins among the identified compounds. Cell viability tests showed that the extract was not toxic to the cells at concentrations up to 50 µg/ml. Treatment with the extract significantly reduced the levels of pro-inflammatory cytokines TNF-α and IL-1β in LPS-induced RAW 264.7 cells, with the most effective concentration being 50 µg/ml. Molecular docking studies further supported the anti-inflammatory potential of the extract by demonstrating its ability to inhibit COX-2 and TNF-alpha. These findings suggest that P. niruri leaf extract has promising anti-inflammatory properties and needs further investigation as a potential treatment for inflammatory diseases. The in silico study provided insights into the molecular interactions between the extract's compounds and inflammatory targets, supporting its anti-inflammatory mechanism of action. https://journal.ipb.ac.id/index.php/hayati/article/view/55992 |
| spellingShingle | Ngurah Intan Wiratmini Made Pharmawati Anak Agung Sagung Alit Sukmaningsih Sentot Joko Raharjo Wahyu Widowati Anti-Inflammatory Activity of Phyllanthus niruri L. Leaf Extract: In Vitro Study on RAW 264.7 Macrophage Cells and In Silico Analysis Hayati Journal of Biosciences |
| title | Anti-Inflammatory Activity of Phyllanthus niruri L. Leaf Extract: In Vitro Study on RAW 264.7 Macrophage Cells and In Silico Analysis |
| title_full | Anti-Inflammatory Activity of Phyllanthus niruri L. Leaf Extract: In Vitro Study on RAW 264.7 Macrophage Cells and In Silico Analysis |
| title_fullStr | Anti-Inflammatory Activity of Phyllanthus niruri L. Leaf Extract: In Vitro Study on RAW 264.7 Macrophage Cells and In Silico Analysis |
| title_full_unstemmed | Anti-Inflammatory Activity of Phyllanthus niruri L. Leaf Extract: In Vitro Study on RAW 264.7 Macrophage Cells and In Silico Analysis |
| title_short | Anti-Inflammatory Activity of Phyllanthus niruri L. Leaf Extract: In Vitro Study on RAW 264.7 Macrophage Cells and In Silico Analysis |
| title_sort | anti inflammatory activity of phyllanthus niruri l leaf extract in vitro study on raw 264 7 macrophage cells and in silico analysis |
| url | https://journal.ipb.ac.id/index.php/hayati/article/view/55992 |
| work_keys_str_mv | AT ngurahintanwiratmini antiinflammatoryactivityofphyllanthusnirurilleafextractinvitrostudyonraw2647macrophagecellsandinsilicoanalysis AT madepharmawati antiinflammatoryactivityofphyllanthusnirurilleafextractinvitrostudyonraw2647macrophagecellsandinsilicoanalysis AT anakagungsagungalitsukmaningsih antiinflammatoryactivityofphyllanthusnirurilleafextractinvitrostudyonraw2647macrophagecellsandinsilicoanalysis AT sentotjokoraharjo antiinflammatoryactivityofphyllanthusnirurilleafextractinvitrostudyonraw2647macrophagecellsandinsilicoanalysis AT wahyuwidowati antiinflammatoryactivityofphyllanthusnirurilleafextractinvitrostudyonraw2647macrophagecellsandinsilicoanalysis |