PEG-Mediated Protoplast Transformation of <i>Penicillium sclerotiorum</i> (scaumcx01): Metabolomic Shifts and Root Colonization Dynamics

PEG-Mediated Protoplast Transformation of <i>Penicillium sclerotiorum</i> (scaumcx01): Metabolomic Shifts and Root Colonization Dynamics

Protoplast-based transformation is a vital tool for genetic studies in fungi, yet no protoplast method existed for <i>P. sclerotiorum</i>-scaumcx01 before this study. Here, we optimized protoplast isolation, regeneration, and transformation efficiency. The highest protoplast yield (6.72...

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Bibliographic Details
Main Authors:	Israt Jahan, Qilin Yang, Zijun Guan, Yihan Wang, Ping Li, Yan Jian
Format:	Article
Language:	English
Published:	MDPI AG 2025-05-01
Series:	Journal of Fungi
Subjects:	<i>Penicillium sclerotiorum</i> protoplast transformation <i>GFP</i> tagging metabolomic analysis plant–fungal interaction
Online Access:	https://www.mdpi.com/2309-608X/11/5/386
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