One-time application of controlled-release blended fertilizer increases rice yield and nitrogen utilization by optimizing root morphological trait distribution and nitrogen uptake

One-time application of controlled-release blended fertilizer (CRBF, a mixture of five nitrogen (N) fertilizers in a certain ratio) can achieve high yield and N use efficiency (NUE) in rice (Oryza sativa L.). However, the effects of CRBF with one-time application on root spatial distribution and phy...

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Main Authors: Yuhui Wang, Shen Gao, Jie Sun, Bin He, Wenjun He, Weike Tao, Xin’ao Tang, Zhi Geng, Zhengyang Wu, Weiwei Li, Zhengrong Jiang, Zhenghui Liu, Yanfeng Ding, Soulaiman Sakr, Pengfu Hou, Ganghua Li
Format: Article
Language:English
Published: KeAi Communications Co., Ltd. 2025-08-01
Series:Crop Journal
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Online Access:http://www.sciencedirect.com/science/article/pii/S2214514125001060
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author Yuhui Wang
Shen Gao
Jie Sun
Bin He
Wenjun He
Weike Tao
Xin’ao Tang
Zhi Geng
Zhengyang Wu
Weiwei Li
Zhengrong Jiang
Zhenghui Liu
Yanfeng Ding
Soulaiman Sakr
Pengfu Hou
Ganghua Li
author_facet Yuhui Wang
Shen Gao
Jie Sun
Bin He
Wenjun He
Weike Tao
Xin’ao Tang
Zhi Geng
Zhengyang Wu
Weiwei Li
Zhengrong Jiang
Zhenghui Liu
Yanfeng Ding
Soulaiman Sakr
Pengfu Hou
Ganghua Li
author_sort Yuhui Wang
collection DOAJ
description One-time application of controlled-release blended fertilizer (CRBF, a mixture of five nitrogen (N) fertilizers in a certain ratio) can achieve high yield and N use efficiency (NUE) in rice (Oryza sativa L.). However, the effects of CRBF with one-time application on root spatial distribution and physiological characteristics remain unclear. We measured the effects of CRBF with one-time application on rice yield, NUE, root morphology and growth, and N uptake capacity in field and root box experiments. Six N treatments were set up: no nitrogen (N0), high-yield three-split application of urea as a control (CK), urea (U) with broadcast, U with side-deep fertilization, CRBF with broadcast, and CRBF with side-deep fertilization. Our findings showed that root characters were positively correlated with yield and NUE. Compared to CK and U treatments, CRBF with one-time applications increased root characters (including root biomass, root N uptake, root activity, and the expression level of ammonium transporters) at tillering and heading stages. The root length, surface area and volume in the 0–10 cm soil layer enhanced under CRBF with one-time applications at tillering stage, and in the 0–20 cm soil layer at the heading stage. This contributed a 5.96%–39.40% and 3.69%–16.87% increase in plant dry matter accumulation and N uptake, and a 2.08%–18.28% and 14.60%–149.57% increase in yield and NUE, in 2022 and 2023, respectively. Taken together, our findings showed that one-time application of CRBF could increase rice yield and NUE by optimizing the root morphology distribution and N uptake.
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spelling doaj-art-11a55a2dd29349d3ac048a53ef3da2042025-08-22T04:56:25ZengKeAi Communications Co., Ltd.Crop Journal2214-51412025-08-011341234124510.1016/j.cj.2025.04.008One-time application of controlled-release blended fertilizer increases rice yield and nitrogen utilization by optimizing root morphological trait distribution and nitrogen uptakeYuhui Wang0Shen Gao1Jie Sun2Bin He3Wenjun He4Weike Tao5Xin’ao Tang6Zhi Geng7Zhengyang Wu8Weiwei Li9Zhengrong Jiang10Zhenghui Liu11Yanfeng Ding12Soulaiman Sakr13Pengfu Hou14Ganghua Li15Sanya Institute of Nanjing Agriculture University, Jiangsu Collaborative Innovation Center for Modern Crop Production, Key Laboratory of Crop Physiology Ecology and Production Management, Nanjing Agricultural University, Nanjing 211800, Jiangsu, China; Institut Agro, University of Angers, INRAE, IRHS, SFR QUASAV, Angers 49000, FranceSanya Institute of Nanjing Agriculture University, Jiangsu Collaborative Innovation Center for Modern Crop Production, Key Laboratory of Crop Physiology Ecology and Production Management, Nanjing Agricultural University, Nanjing 211800, Jiangsu, ChinaSanya Institute of Nanjing Agriculture University, Jiangsu Collaborative Innovation Center for Modern Crop Production, Key Laboratory of Crop Physiology Ecology and Production Management, Nanjing Agricultural University, Nanjing 211800, Jiangsu, ChinaSanya Institute of Nanjing Agriculture University, Jiangsu Collaborative Innovation Center for Modern Crop Production, Key Laboratory of Crop Physiology Ecology and Production Management, Nanjing Agricultural University, Nanjing 211800, Jiangsu, ChinaTaizhou Academy of Agricultural Sciences, Taizhou 317000, Zhejiang, ChinaSanya Institute of Nanjing Agriculture University, Jiangsu Collaborative Innovation Center for Modern Crop Production, Key Laboratory of Crop Physiology Ecology and Production Management, Nanjing Agricultural University, Nanjing 211800, Jiangsu, ChinaSanya Institute of Nanjing Agriculture University, Jiangsu Collaborative Innovation Center for Modern Crop Production, Key Laboratory of Crop Physiology Ecology and Production Management, Nanjing Agricultural University, Nanjing 211800, Jiangsu, ChinaSanya Institute of Nanjing Agriculture University, Jiangsu Collaborative Innovation Center for Modern Crop Production, Key Laboratory of Crop Physiology Ecology and Production Management, Nanjing Agricultural University, Nanjing 211800, Jiangsu, ChinaSanya Institute of Nanjing Agriculture University, Jiangsu Collaborative Innovation Center for Modern Crop Production, Key Laboratory of Crop Physiology Ecology and Production Management, Nanjing Agricultural University, Nanjing 211800, Jiangsu, ChinaSanya Institute of Nanjing Agriculture University, Jiangsu Collaborative Innovation Center for Modern Crop Production, Key Laboratory of Crop Physiology Ecology and Production Management, Nanjing Agricultural University, Nanjing 211800, Jiangsu, China; Key Laboratory of Agro-Environment in Downstream of Yangze Plain, Ministry of Agriculture and Rura Affairs, Nanjing 210014, Jiangsu, ChinaSanya Institute of Nanjing Agriculture University, Jiangsu Collaborative Innovation Center for Modern Crop Production, Key Laboratory of Crop Physiology Ecology and Production Management, Nanjing Agricultural University, Nanjing 211800, Jiangsu, China; Institut Agro, University of Angers, INRAE, IRHS, SFR QUASAV, Angers 49000, FranceSanya Institute of Nanjing Agriculture University, Jiangsu Collaborative Innovation Center for Modern Crop Production, Key Laboratory of Crop Physiology Ecology and Production Management, Nanjing Agricultural University, Nanjing 211800, Jiangsu, ChinaSanya Institute of Nanjing Agriculture University, Jiangsu Collaborative Innovation Center for Modern Crop Production, Key Laboratory of Crop Physiology Ecology and Production Management, Nanjing Agricultural University, Nanjing 211800, Jiangsu, ChinaInstitut Agro, University of Angers, INRAE, IRHS, SFR QUASAV, Angers 49000, FranceKey Laboratory of Agro-Environment in Downstream of Yangze Plain, Ministry of Agriculture and Rura Affairs, Nanjing 210014, Jiangsu, China; Corresponding authors.Sanya Institute of Nanjing Agriculture University, Jiangsu Collaborative Innovation Center for Modern Crop Production, Key Laboratory of Crop Physiology Ecology and Production Management, Nanjing Agricultural University, Nanjing 211800, Jiangsu, China; Corresponding authors.One-time application of controlled-release blended fertilizer (CRBF, a mixture of five nitrogen (N) fertilizers in a certain ratio) can achieve high yield and N use efficiency (NUE) in rice (Oryza sativa L.). However, the effects of CRBF with one-time application on root spatial distribution and physiological characteristics remain unclear. We measured the effects of CRBF with one-time application on rice yield, NUE, root morphology and growth, and N uptake capacity in field and root box experiments. Six N treatments were set up: no nitrogen (N0), high-yield three-split application of urea as a control (CK), urea (U) with broadcast, U with side-deep fertilization, CRBF with broadcast, and CRBF with side-deep fertilization. Our findings showed that root characters were positively correlated with yield and NUE. Compared to CK and U treatments, CRBF with one-time applications increased root characters (including root biomass, root N uptake, root activity, and the expression level of ammonium transporters) at tillering and heading stages. The root length, surface area and volume in the 0–10 cm soil layer enhanced under CRBF with one-time applications at tillering stage, and in the 0–20 cm soil layer at the heading stage. This contributed a 5.96%–39.40% and 3.69%–16.87% increase in plant dry matter accumulation and N uptake, and a 2.08%–18.28% and 14.60%–149.57% increase in yield and NUE, in 2022 and 2023, respectively. Taken together, our findings showed that one-time application of CRBF could increase rice yield and NUE by optimizing the root morphology distribution and N uptake.http://www.sciencedirect.com/science/article/pii/S2214514125001060Rice (Oryza sativa L.)Controlled release blended fertilizerRootYieldNitrogen utilization
spellingShingle Yuhui Wang
Shen Gao
Jie Sun
Bin He
Wenjun He
Weike Tao
Xin’ao Tang
Zhi Geng
Zhengyang Wu
Weiwei Li
Zhengrong Jiang
Zhenghui Liu
Yanfeng Ding
Soulaiman Sakr
Pengfu Hou
Ganghua Li
One-time application of controlled-release blended fertilizer increases rice yield and nitrogen utilization by optimizing root morphological trait distribution and nitrogen uptake
Crop Journal
Rice (Oryza sativa L.)
Controlled release blended fertilizer
Root
Yield
Nitrogen utilization
title One-time application of controlled-release blended fertilizer increases rice yield and nitrogen utilization by optimizing root morphological trait distribution and nitrogen uptake
title_full One-time application of controlled-release blended fertilizer increases rice yield and nitrogen utilization by optimizing root morphological trait distribution and nitrogen uptake
title_fullStr One-time application of controlled-release blended fertilizer increases rice yield and nitrogen utilization by optimizing root morphological trait distribution and nitrogen uptake
title_full_unstemmed One-time application of controlled-release blended fertilizer increases rice yield and nitrogen utilization by optimizing root morphological trait distribution and nitrogen uptake
title_short One-time application of controlled-release blended fertilizer increases rice yield and nitrogen utilization by optimizing root morphological trait distribution and nitrogen uptake
title_sort one time application of controlled release blended fertilizer increases rice yield and nitrogen utilization by optimizing root morphological trait distribution and nitrogen uptake
topic Rice (Oryza sativa L.)
Controlled release blended fertilizer
Root
Yield
Nitrogen utilization
url http://www.sciencedirect.com/science/article/pii/S2214514125001060
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