Engineering Conductive Hydrogels with Tissue‐like Properties: A 3D Bioprinting and Enzymatic Polymerization Approach
Hydrogels are promising materials for medical devices interfacing with neural tissues due to their similar mechanical properties. Traditional hydrogel‐based bio‐interfaces lack sufficient electrical conductivity, relying on low ionic conductivity, which limits signal transduction distance. Conductin...
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Wiley-VCH
2024-11-01
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| Online Access: | https://doi.org/10.1002/smsc.202400290 |
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| author | Changbai Li Sajjad Naeimipour Fatemeh Rasti Boroojeni Tobias Abrahamsson Xenofon Strakosas Yangpeiqi Yi Rebecka Rilemark Caroline Lindholm Venkata K. Perla Chiara Musumeci Yuyang Li Hanne Biesmans Marios Savvakis Eva Olsson Klas Tybrandt Mary J. Donahue Jennifer Y. Gerasimov Robert Selegård Magnus Berggren Daniel Aili Daniel T. Simon |
| author_facet | Changbai Li Sajjad Naeimipour Fatemeh Rasti Boroojeni Tobias Abrahamsson Xenofon Strakosas Yangpeiqi Yi Rebecka Rilemark Caroline Lindholm Venkata K. Perla Chiara Musumeci Yuyang Li Hanne Biesmans Marios Savvakis Eva Olsson Klas Tybrandt Mary J. Donahue Jennifer Y. Gerasimov Robert Selegård Magnus Berggren Daniel Aili Daniel T. Simon |
| author_sort | Changbai Li |
| collection | DOAJ |
| description | Hydrogels are promising materials for medical devices interfacing with neural tissues due to their similar mechanical properties. Traditional hydrogel‐based bio‐interfaces lack sufficient electrical conductivity, relying on low ionic conductivity, which limits signal transduction distance. Conducting polymer hydrogels offer enhanced ionic and electronic conductivities and biocompatibility but often face challenges in processability and require aggressive polymerization methods. Herein, we demonstrate in situ enzymatic polymerization of π‐conjugated monomers in a hyaluronan (HA)‐based hydrogel bioink to create cell‐compatible, electrically conductive hydrogel structures. These structures were fabricated using 3D bioprinting of HA‐based bioinks loaded with conjugated monomers, followed by enzymatic polymerization via horseradish peroxidase. This process increased the hydrogels’ stiffness from about 0.6 to 1.5 kPa and modified their electroactivity. The components and polymerization process were well‐tolerated by human primary dermal fibroblasts and PC12 cells. This work presents a novel method to fabricate cytocompatible and conductive hydrogels suitable for bioprinting. These hybrid materials combine tissue‐like mechanical properties with mixed ionic and electronic conductivity, providing new ways to use electricity to influence cell behavior in a native‐like microenvironment. |
| format | Article |
| id | doaj-art-0e7ea82d9a494d00b9dc6fa0a6937ece |
| institution | Kabale University |
| issn | 2688-4046 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Wiley-VCH |
| record_format | Article |
| series | Small Science |
| spelling | doaj-art-0e7ea82d9a494d00b9dc6fa0a6937ece2024-11-11T15:33:38ZengWiley-VCHSmall Science2688-40462024-11-01411n/an/a10.1002/smsc.202400290Engineering Conductive Hydrogels with Tissue‐like Properties: A 3D Bioprinting and Enzymatic Polymerization ApproachChangbai Li0Sajjad Naeimipour1Fatemeh Rasti Boroojeni2Tobias Abrahamsson3Xenofon Strakosas4Yangpeiqi Yi5Rebecka Rilemark6Caroline Lindholm7Venkata K. Perla8Chiara Musumeci9Yuyang Li10Hanne Biesmans11Marios Savvakis12Eva Olsson13Klas Tybrandt14Mary J. Donahue15Jennifer Y. Gerasimov16Robert Selegård17Magnus Berggren18Daniel Aili19Daniel T. Simon20Laboratory of Organic Electronics Department of Science and Technology Linköping University 60174 Norrköping SwedenLaboratory of Molecular Materials Division of Biophysics and Bioengineering Department of Physics, Chemistry and Biology Linköping University 58183 Linköping SwedenLaboratory of Molecular Materials Division of Biophysics and Bioengineering Department of Physics, Chemistry and Biology Linköping University 58183 Linköping SwedenLaboratory of Organic Electronics Department of Science and Technology Linköping University 60174 Norrköping SwedenLaboratory of Organic Electronics Department of Science and Technology Linköping University 60174 Norrköping SwedenLaboratory of Organic Electronics Department of Science and Technology Linköping University 60174 Norrköping SwedenDepartment of Physics Chalmers University of Technology 41296 Göteborg SwedenLaboratory of Organic Electronics Department of Science and Technology Linköping University 60174 Norrköping SwedenLaboratory of Organic Electronics Department of Science and Technology Linköping University 60174 Norrköping SwedenLaboratory of Organic Electronics Department of Science and Technology Linköping University 60174 Norrköping SwedenLaboratory of Organic Electronics Department of Science and Technology Linköping University 60174 Norrköping SwedenLaboratory of Organic Electronics Department of Science and Technology Linköping University 60174 Norrköping SwedenLaboratory of Organic Electronics Department of Science and Technology Linköping University 60174 Norrköping SwedenDepartment of Physics Chalmers University of Technology 41296 Göteborg SwedenLaboratory of Organic Electronics Department of Science and Technology Linköping University 60174 Norrköping SwedenLaboratory of Organic Electronics Department of Science and Technology Linköping University 60174 Norrköping SwedenLaboratory of Organic Electronics Department of Science and Technology Linköping University 60174 Norrköping SwedenLaboratory of Molecular Materials Division of Biophysics and Bioengineering Department of Physics, Chemistry and Biology Linköping University 58183 Linköping SwedenLaboratory of Organic Electronics Department of Science and Technology Linköping University 60174 Norrköping SwedenLaboratory of Molecular Materials Division of Biophysics and Bioengineering Department of Physics, Chemistry and Biology Linköping University 58183 Linköping SwedenLaboratory of Organic Electronics Department of Science and Technology Linköping University 60174 Norrköping SwedenHydrogels are promising materials for medical devices interfacing with neural tissues due to their similar mechanical properties. Traditional hydrogel‐based bio‐interfaces lack sufficient electrical conductivity, relying on low ionic conductivity, which limits signal transduction distance. Conducting polymer hydrogels offer enhanced ionic and electronic conductivities and biocompatibility but often face challenges in processability and require aggressive polymerization methods. Herein, we demonstrate in situ enzymatic polymerization of π‐conjugated monomers in a hyaluronan (HA)‐based hydrogel bioink to create cell‐compatible, electrically conductive hydrogel structures. These structures were fabricated using 3D bioprinting of HA‐based bioinks loaded with conjugated monomers, followed by enzymatic polymerization via horseradish peroxidase. This process increased the hydrogels’ stiffness from about 0.6 to 1.5 kPa and modified their electroactivity. The components and polymerization process were well‐tolerated by human primary dermal fibroblasts and PC12 cells. This work presents a novel method to fabricate cytocompatible and conductive hydrogels suitable for bioprinting. These hybrid materials combine tissue‐like mechanical properties with mixed ionic and electronic conductivity, providing new ways to use electricity to influence cell behavior in a native‐like microenvironment.https://doi.org/10.1002/smsc.2024002903D printingcell scaffoldconducting polymerin vitropolymerization |
| spellingShingle | Changbai Li Sajjad Naeimipour Fatemeh Rasti Boroojeni Tobias Abrahamsson Xenofon Strakosas Yangpeiqi Yi Rebecka Rilemark Caroline Lindholm Venkata K. Perla Chiara Musumeci Yuyang Li Hanne Biesmans Marios Savvakis Eva Olsson Klas Tybrandt Mary J. Donahue Jennifer Y. Gerasimov Robert Selegård Magnus Berggren Daniel Aili Daniel T. Simon Engineering Conductive Hydrogels with Tissue‐like Properties: A 3D Bioprinting and Enzymatic Polymerization Approach Small Science 3D printing cell scaffold conducting polymer in vitro polymerization |
| title | Engineering Conductive Hydrogels with Tissue‐like Properties: A 3D Bioprinting and Enzymatic Polymerization Approach |
| title_full | Engineering Conductive Hydrogels with Tissue‐like Properties: A 3D Bioprinting and Enzymatic Polymerization Approach |
| title_fullStr | Engineering Conductive Hydrogels with Tissue‐like Properties: A 3D Bioprinting and Enzymatic Polymerization Approach |
| title_full_unstemmed | Engineering Conductive Hydrogels with Tissue‐like Properties: A 3D Bioprinting and Enzymatic Polymerization Approach |
| title_short | Engineering Conductive Hydrogels with Tissue‐like Properties: A 3D Bioprinting and Enzymatic Polymerization Approach |
| title_sort | engineering conductive hydrogels with tissue like properties a 3d bioprinting and enzymatic polymerization approach |
| topic | 3D printing cell scaffold conducting polymer in vitro polymerization |
| url | https://doi.org/10.1002/smsc.202400290 |
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