Genotyping by Amplicon Sequencing (GBAS) With Newly Developed SSR and EPIC Markers Reveals Structure in Populations of the Green Toad (Bufotes viridis) Across Rural and Urban Environments

Genotyping by Amplicon Sequencing (GBAS) With Newly Developed SSR and EPIC Markers Reveals Structure in Populations of the Green Toad (Bufotes viridis) Across Rural and Urban Environments

ABSTRACT Microsatellites (SSRs) are reliable markers for population genetic analyses but often suffer from null alleles caused by mutations in primer binding sites. Exon‐primed intron‐crossing (EPIC) markers address these limitations and serve as a complementary tool. In this study, we developed SSR...

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Main Authors: Vincent Kendlbacher, Yoko Philipina Krenn, Thapasya Vijayan, Christina Rupprecht, Magdalena Spießberger, Manuel Curto, Lukas Landler, Harald Meimberg
Format: Article
Language:English
Published: Wiley 2025-07-01
Series:Ecology and Evolution
Subjects:
amphibian
conservation genetics
genetic markers
population genetics
urban environment
Online Access:https://doi.org/10.1002/ece3.71652
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https://doi.org/10.1002/ece3.71652

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