Transcriptome analysis reveals the anticancer effects of fenbendazole on ovarian cancer: an in vitro and in vivo study
Abstract New treatment strategies for ovarian cancer, which is the deadliest female reproductive tract malignancy, are urgently needed. Here, we investigated the anticancer effects of fenbendazole (FBZ), a benzimidazole compound, on the regulation of apoptosis and mitotic catastrophe in A2780 and SK...
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2024-12-01
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| Series: | BMC Cancer |
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| Online Access: | https://doi.org/10.1186/s12885-024-13361-9 |
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| author | Xin Wang Wenda Tian Ning Wang Xiangqun Yang Zhenyan Liu Lvzhou Li Taoyu Zhao Chuanlin Wang Hongping Zhang Hongying Yang Yue Jia |
| author_facet | Xin Wang Wenda Tian Ning Wang Xiangqun Yang Zhenyan Liu Lvzhou Li Taoyu Zhao Chuanlin Wang Hongping Zhang Hongying Yang Yue Jia |
| author_sort | Xin Wang |
| collection | DOAJ |
| description | Abstract New treatment strategies for ovarian cancer, which is the deadliest female reproductive tract malignancy, are urgently needed. Here, we investigated the anticancer effects of fenbendazole (FBZ), a benzimidazole compound, on the regulation of apoptosis and mitotic catastrophe in A2780 and SKOV3 human epithelial ovarian cancer cells. Functional experiments, including Cell Counting Kit 8 (CCK-8), colony formation, and flow cytometry assays, were conducted to explore the effects of FBZ on the malignant biological behavior of A2780 and SKOV3 cells. RNA sequencing and western blotting were utilized to elucidate the underlying mechanisms by which FBZ affects cell apoptosis. We found that FBZ inhibited the proliferation and promoted the apoptosis of ovarian cancer cells in a dose-dependent manner. Furthermore, we reported the transcriptome profiling of FBZ-treated SKOV3 ovarian cancer cells. In all, 1747 differentially expressed genes (DEGs) were identified, including 944 downregulated and 803 upregulated genes. KEGG enrichment and Reactome enrichment analyses revealed that the DEGs were associated mainly with mitosis- and cell cycle-related pathways. Additionally, we found that FBZ may promote apoptosis via mitotic catastrophe. Finally, oral administration of FBZ inhibited tumor growth in a mouse model of xenograft ovarian cancer. Overall, these findings suggest that FBZ has therapeutic potential for the treatment of ovarian cancer. |
| format | Article |
| id | doaj-art-0d434e7371b74c6e8e5c383f20b87846 |
| institution | Kabale University |
| issn | 1471-2407 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Cancer |
| spelling | doaj-art-0d434e7371b74c6e8e5c383f20b878462025-01-05T12:33:13ZengBMCBMC Cancer1471-24072024-12-0124111410.1186/s12885-024-13361-9Transcriptome analysis reveals the anticancer effects of fenbendazole on ovarian cancer: an in vitro and in vivo studyXin Wang0Wenda Tian1Ning Wang2Xiangqun Yang3Zhenyan Liu4Lvzhou Li5Taoyu Zhao6Chuanlin Wang7Hongping Zhang8Hongying Yang9Yue Jia10Department of Gynecology, Yunnan Cancer Hospital, The Third Affiliated Hospital of Kunming Medical University, Peking University Cancer Hospital YunnanDepartment of Gynecology, Yunnan Cancer Hospital, The Third Affiliated Hospital of Kunming Medical University, Peking University Cancer Hospital YunnanDepartment of Obstetrics and Gynecology, Dehong Affiliated Hospital of Kunming Medical University, Dehong People’s Hospital of Yunnan ProvinceDepartment of Gynecology, Yunnan Cancer Hospital, The Third Affiliated Hospital of Kunming Medical University, Peking University Cancer Hospital YunnanDepartment of Gynecology, Yunnan Cancer Hospital, The Third Affiliated Hospital of Kunming Medical University, Peking University Cancer Hospital YunnanDepartment of Obstetrics and Gynecology, Dehong Affiliated Hospital of Kunming Medical University, Dehong People’s Hospital of Yunnan ProvinceDepartment of Obstetrics and Gynecology, Dehong Affiliated Hospital of Kunming Medical University, Dehong People’s Hospital of Yunnan ProvinceDepartment of Clinical Nutrition, Yunnan Cancer Hospital, The Third Affiliated Hospital of Kunming Medical University, Peking University Cancer Hospital YunnanDepartment of Gynecology, Yunnan Cancer Hospital, The Third Affiliated Hospital of Kunming Medical University, Peking University Cancer Hospital YunnanDepartment of Gynecology, Yunnan Cancer Hospital, The Third Affiliated Hospital of Kunming Medical University, Peking University Cancer Hospital YunnanDepartment of Gynecology, Yunnan Cancer Hospital, The Third Affiliated Hospital of Kunming Medical University, Peking University Cancer Hospital YunnanAbstract New treatment strategies for ovarian cancer, which is the deadliest female reproductive tract malignancy, are urgently needed. Here, we investigated the anticancer effects of fenbendazole (FBZ), a benzimidazole compound, on the regulation of apoptosis and mitotic catastrophe in A2780 and SKOV3 human epithelial ovarian cancer cells. Functional experiments, including Cell Counting Kit 8 (CCK-8), colony formation, and flow cytometry assays, were conducted to explore the effects of FBZ on the malignant biological behavior of A2780 and SKOV3 cells. RNA sequencing and western blotting were utilized to elucidate the underlying mechanisms by which FBZ affects cell apoptosis. We found that FBZ inhibited the proliferation and promoted the apoptosis of ovarian cancer cells in a dose-dependent manner. Furthermore, we reported the transcriptome profiling of FBZ-treated SKOV3 ovarian cancer cells. In all, 1747 differentially expressed genes (DEGs) were identified, including 944 downregulated and 803 upregulated genes. KEGG enrichment and Reactome enrichment analyses revealed that the DEGs were associated mainly with mitosis- and cell cycle-related pathways. Additionally, we found that FBZ may promote apoptosis via mitotic catastrophe. Finally, oral administration of FBZ inhibited tumor growth in a mouse model of xenograft ovarian cancer. Overall, these findings suggest that FBZ has therapeutic potential for the treatment of ovarian cancer.https://doi.org/10.1186/s12885-024-13361-9FenbendazoleOvarian cancerApoptosisMitotic catastrophe |
| spellingShingle | Xin Wang Wenda Tian Ning Wang Xiangqun Yang Zhenyan Liu Lvzhou Li Taoyu Zhao Chuanlin Wang Hongping Zhang Hongying Yang Yue Jia Transcriptome analysis reveals the anticancer effects of fenbendazole on ovarian cancer: an in vitro and in vivo study BMC Cancer Fenbendazole Ovarian cancer Apoptosis Mitotic catastrophe |
| title | Transcriptome analysis reveals the anticancer effects of fenbendazole on ovarian cancer: an in vitro and in vivo study |
| title_full | Transcriptome analysis reveals the anticancer effects of fenbendazole on ovarian cancer: an in vitro and in vivo study |
| title_fullStr | Transcriptome analysis reveals the anticancer effects of fenbendazole on ovarian cancer: an in vitro and in vivo study |
| title_full_unstemmed | Transcriptome analysis reveals the anticancer effects of fenbendazole on ovarian cancer: an in vitro and in vivo study |
| title_short | Transcriptome analysis reveals the anticancer effects of fenbendazole on ovarian cancer: an in vitro and in vivo study |
| title_sort | transcriptome analysis reveals the anticancer effects of fenbendazole on ovarian cancer an in vitro and in vivo study |
| topic | Fenbendazole Ovarian cancer Apoptosis Mitotic catastrophe |
| url | https://doi.org/10.1186/s12885-024-13361-9 |
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