Molecular detection and genetic variability of Fusarium oxysporum mating types
The genus Fusarium comprises a vast number of cosmopolitan species, many of which are important pathogens of crops of agronomic interest. Molecular analysis of mating type (MAT) genes is a useful tool to study reproductive lifestyles and relationships between species. The aim of the present researc...
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| Format: | Article |
| Language: | English |
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Escuela Nacional de Ciencias Biológicas
2020-10-01
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| Series: | Polibotánica |
| Subjects: | |
| Online Access: | https://polibotanica.mx/index.php/polibotanica/article/view/602 |
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| Summary: | The genus Fusarium comprises a vast number of cosmopolitan species, many of which are important pathogens of crops of agronomic interest. Molecular analysis of mating type (MAT) genes is a useful tool to study reproductive lifestyles and relationships between species. The aim of the present research was to determine by PCR the type of MAT idiomorphs (MAT1-1 and/or MAT1-2) in Fusarium oxysporum, F. nygamai, F. thapsinum and F. verticillioides isolates obtained across 20 geographical regions of the central zone of Mexico, and to evaluate their sequence variability. PCR reactions from 110 isolates revealed that 66 Fusarium isolates presented the MAT1-1 idiomorph and 44 isolates presented the MAT1-2 idiomorph, but no isolate showed both idiomorphs. MAT1-1 and MAT1-2 idiomorph, from the same or different Fusarium species, coincided in the same field of several geographic regions. Selected sequences of the two idiomorphs showed greater divergence between species than within the same species. These changes were greater in the noncoding regions than in the coding regions. In general, these findings will help to better understand the genetic variability and, ideally, improve the management practices of crop diseases.
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| ISSN: | 1405-2768 2395-9525 |