A massively parallel reporter assay library to screen short synthetic promoters in mammalian cells

Abstract Cellular responses to stimuli underpin discoveries in drug development, synthetic biology, and general life sciences. We introduce a library comprising 6144 synthetic promoters, each shorter than 250 bp, designed as transcriptional readouts of cellular stimulus responses in massively parall...

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Bibliographic Details
Main Authors: Adam M. Zahm, William S. Owens, Samuel R. Himes, Braden S. Fallon, Kathleen E. Rondem, Alexa N. Gormick, Joshua S. Bloom, Sriram Kosuri, Henry Chan, Justin G. English
Format: Article
Language:English
Published: Nature Portfolio 2024-11-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-024-54502-9
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