Quantitative benchmarking of nuclear segmentation algorithms in multiplexed immunofluorescence imaging for translational studies

Abstract Multiplexed imaging techniques require identifying different cell types in the tissue. To utilize their potential for cellular and molecular analysis, high throughput and accurate analytical approaches are needed in parsing vast amounts of data, particularly in clinical settings. Nuclear se...

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Bibliographic Details
Main Authors: Abishek Sankaranarayanan, Georgii Khachaturov, Kimberly S. Smythe, Shachi Mittal
Format: Article
Language:English
Published: Nature Portfolio 2025-05-01
Series:Communications Biology
Online Access:https://doi.org/10.1038/s42003-025-08184-8
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