Establishment of a novel engineering breeding technology for cytoplasmic male sterility

Establishment of a novel engineering breeding technology for cytoplasmic male sterility

The three-line hybrid rice system, which relies on cytoplasmic male sterility (CMS), faces challenges in breeding efficiency. To address these challenges, we developed CMS engineering breeding technology (CEBT). First, we constructed a complementation vector containing the fertility restoration modu...

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Bibliographic Details
Main Authors: Na Xu, Dong Yu, Longhui Zhang, Tiankang Wang, Zhongxiao Hu, Quanqiang Lei, Yingxin Qiu, Zheming Yuan, Shufeng Song, Li Li
Format: Article
Language:English
Published: KeAi Communications Co., Ltd. 2025-08-01
Series:Crop Journal
Subjects:
Three-line hybrid rice
CMS-WA
Rf gene
CEBT
Reproductive lines
Online Access:http://www.sciencedirect.com/science/article/pii/S2214514125001096
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Summary:The three-line hybrid rice system, which relies on cytoplasmic male sterility (CMS), faces challenges in breeding efficiency. To address these challenges, we developed CMS engineering breeding technology (CEBT). First, we constructed a complementation vector containing the fertility restoration module Double Rf4-Rf20, sorting marker gene DsRed2, and pollen lethal gene ZmAA1, then transformed this into a CMS line. We obtained reproductive lines without alterations to main agronomic traits, which can produce engineering sterile lines (ESL) and reproductive line seeds at a 1:1 ratio by self-crossing. CEBT converts the traditional three-line system into an efficient ‘new two-line’ hybrid framework: the reproductive line is improved while simultaneously improving the ESL, which significantly shortens the breeding process for three-line hybrid rice.
ISSN:2214-5141

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