Protocol for high-quality RNA sequencing, cell surface protein analysis, and genotyping in single cells using TARGET-seq+

Protocol for high-quality RNA sequencing, cell surface protein analysis, and genotyping in single cells using TARGET-seq+

Summary: Studying the consequences of somatic mutations in pre-malignant and cancerous tissues is challenging due to noise in single-cell transcriptome data and difficulty in identifying the clonal identity of single cells. We optimized TARGET-seq to develop TARGET-seq+, which combines RNA sequencin...

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Bibliographic Details
Main Authors: N. Asger Jakobsen, Sven Turkalj, Paresh Vyas
Format: Article
Language:English
Published: Elsevier 2025-06-01
Series:STAR Protocols
Subjects:
Flow Cytometry
Gene Expression
Genomics
Molecular Biology
RNA-seq
Sequencing
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166725002382
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http://www.sciencedirect.com/science/article/pii/S2666166725002382

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