Exploring the Interplay Between Senescent Osteocytes and Bone Remodeling in Young Rodents
This study identifies senescent osteocytes in the femur and tibia of young rodents and explores their role in bone remodeling. The proximity of osteoclasts to senescent osteocytes was observed, which is a new finding. Cultured osteocytes, sorted using a podoplanin antibody in FACS, exhibited osteocy...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2024-01-01
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| Series: | Journal of Aging Research |
| Online Access: | http://dx.doi.org/10.1155/2024/4213141 |
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| _version_ | 1846159077002969088 |
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| author | Insun Song Pil-Jong Kim Yong Jun Choi Yoon-Sok Chung Soonchul Lee Jeong-Hwa Baek Kyung Mi Woo |
| author_facet | Insun Song Pil-Jong Kim Yong Jun Choi Yoon-Sok Chung Soonchul Lee Jeong-Hwa Baek Kyung Mi Woo |
| author_sort | Insun Song |
| collection | DOAJ |
| description | This study identifies senescent osteocytes in the femur and tibia of young rodents and explores their role in bone remodeling. The proximity of osteoclasts to senescent osteocytes was observed, which is a new finding. Cultured osteocytes, sorted using a podoplanin antibody in FACS, exhibited osteocytic characteristics and increased senescence-related genes. Senescent osteocytes secreted cytokines associated with senescence, remodeling, and inflammation. Notably, IGF1 and MMP2 were elevated in podoplanin-positive (pdpn+) osteocytes. Migration assays demonstrated significant osteoclast precursor migration towards senescent osteocytes, further confirmed by co-culture experiments leading to osteoclast differentiation. These findings suggest that senescent osteocytes have a pivotal role in initiating bone resorption, with recruitment of osteoclast precursors during early bone remodeling stages. In conclusion, our research enhances our understanding of complicated bone remodeling mechanisms and bone homeostasis. |
| format | Article |
| id | doaj-art-0896cf565c9a4663898fc0b85ed66cc7 |
| institution | Kabale University |
| issn | 2090-2212 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Aging Research |
| spelling | doaj-art-0896cf565c9a4663898fc0b85ed66cc72024-11-24T00:00:04ZengWileyJournal of Aging Research2090-22122024-01-01202410.1155/2024/4213141Exploring the Interplay Between Senescent Osteocytes and Bone Remodeling in Young RodentsInsun Song0Pil-Jong Kim1Yong Jun Choi2Yoon-Sok Chung3Soonchul Lee4Jeong-Hwa Baek5Kyung Mi Woo6Dental Research InstituteSchool of Dentistry and Dental Research InstituteDepartment of Endocrinology and MetabolismDepartment of Endocrinology and MetabolismDepartment of Orthopaedic SurgeryDental Research InstituteDental Research InstituteThis study identifies senescent osteocytes in the femur and tibia of young rodents and explores their role in bone remodeling. The proximity of osteoclasts to senescent osteocytes was observed, which is a new finding. Cultured osteocytes, sorted using a podoplanin antibody in FACS, exhibited osteocytic characteristics and increased senescence-related genes. Senescent osteocytes secreted cytokines associated with senescence, remodeling, and inflammation. Notably, IGF1 and MMP2 were elevated in podoplanin-positive (pdpn+) osteocytes. Migration assays demonstrated significant osteoclast precursor migration towards senescent osteocytes, further confirmed by co-culture experiments leading to osteoclast differentiation. These findings suggest that senescent osteocytes have a pivotal role in initiating bone resorption, with recruitment of osteoclast precursors during early bone remodeling stages. In conclusion, our research enhances our understanding of complicated bone remodeling mechanisms and bone homeostasis.http://dx.doi.org/10.1155/2024/4213141 |
| spellingShingle | Insun Song Pil-Jong Kim Yong Jun Choi Yoon-Sok Chung Soonchul Lee Jeong-Hwa Baek Kyung Mi Woo Exploring the Interplay Between Senescent Osteocytes and Bone Remodeling in Young Rodents Journal of Aging Research |
| title | Exploring the Interplay Between Senescent Osteocytes and Bone Remodeling in Young Rodents |
| title_full | Exploring the Interplay Between Senescent Osteocytes and Bone Remodeling in Young Rodents |
| title_fullStr | Exploring the Interplay Between Senescent Osteocytes and Bone Remodeling in Young Rodents |
| title_full_unstemmed | Exploring the Interplay Between Senescent Osteocytes and Bone Remodeling in Young Rodents |
| title_short | Exploring the Interplay Between Senescent Osteocytes and Bone Remodeling in Young Rodents |
| title_sort | exploring the interplay between senescent osteocytes and bone remodeling in young rodents |
| url | http://dx.doi.org/10.1155/2024/4213141 |
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