A single‐cell RNA labeling strategy for measuring stress response upon tissue dissociation

A single‐cell RNA labeling strategy for measuring stress response upon tissue dissociation

Abstract Tissue dissociation, a crucial step in single‐cell sample preparation, can alter the transcriptional state of a sample through the intrinsic cellular stress response. Here we demonstrate a general approach for measuring transcriptional response during sample preparation. In our method, tran...

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Bibliographic Details
Main Authors: Anika Neuschulz, Olga Bakina, Victor Badillo‐Lisakowski, Pedro Olivares‐Chauvet, Thomas Conrad, Michael Gotthardt, Helmut Kettenmann, Jan Philipp Junker
Format: Article
Language:English
Published: Springer Nature 2022-12-01
Series:Molecular Systems Biology
Subjects:
cellular stress response
RNA labeling
single‐cell transcriptomics
SLAM‐seq
tissue dissociation
Online Access:https://doi.org/10.15252/msb.202211147
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https://doi.org/10.15252/msb.202211147

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