Dual-sided centripetal microgrooved poly (D,L-lactide-co-caprolactone) disk encased in immune-regulating hydrogels for enhanced bone regeneration
Well-designed artificial scaffolds are urgently needed due to the limited self-repair capacity of bone, which hampers effective regeneration in critical defects. Optimal scaffolds must provide physical guidance to recruit cells and immune regulation to improve the regenerative microenvironment. This...
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Elsevier
2025-02-01
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| Series: | Materials Today Bio |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590006424004976 |
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| author | You Wu Xiaokun Yue Ying Zhang Ning Yu Chengyan Ge Rui Liu Zhongying Duan Lilong Gao Xinlong Zang Xin Sun Deteng Zhang |
| author_facet | You Wu Xiaokun Yue Ying Zhang Ning Yu Chengyan Ge Rui Liu Zhongying Duan Lilong Gao Xinlong Zang Xin Sun Deteng Zhang |
| author_sort | You Wu |
| collection | DOAJ |
| description | Well-designed artificial scaffolds are urgently needed due to the limited self-repair capacity of bone, which hampers effective regeneration in critical defects. Optimal scaffolds must provide physical guidance to recruit cells and immune regulation to improve the regenerative microenvironment. This study presents a novel scaffold composed of dual-sided centripetal microgrooved poly(D,L-lactide-co-caprolactone) (PLCL) film combined with a dynamic hydrogel containing prednisolone (PLS)-loaded Prussian blue nanoparticles (PB@PLS). The microgrooves on the surface of the PLCL film were imprinted using a micropatterned polydimethylsiloxane (PDMS) template. Following aminolysis, the PLCL film was covalently grafted with the EM-7 peptide via glutaraldehyde. Functional group analysis, surface morphology and hydrophilicity were evaluated using X-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM), and an optical contact angle measuring instrument, respectively. Bone regeneration-related cells (e.g., bone marrow mesenchymal stem cells, macrophages, Schwann cells, and endothelial cells) cultured on PLCL films tended to align along the stripes and migrate from the periphery toward the center region in vitro. Subsequently, the PLCL film was encapsulated in an immune-regulating hydrogel synthesized from thiol-modified gelatin and Cu2+ in the presence of PB@PLS nanoparticles, which demonstrated excellent antioxidant properties. This scaffold significantly accelerated critical-sized bone regeneration, as evidenced by an increase in the volume of newly formed bone and histological images in vivo. This innovative approach holds substantial promise for clinical applications in bone regeneration and broader tissue repair. |
| format | Article |
| id | doaj-art-06ce1e36a7474de9a3a4865c8f850184 |
| institution | Kabale University |
| issn | 2590-0064 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials Today Bio |
| spelling | doaj-art-06ce1e36a7474de9a3a4865c8f8501842025-01-17T04:52:13ZengElsevierMaterials Today Bio2590-00642025-02-0130101436Dual-sided centripetal microgrooved poly (D,L-lactide-co-caprolactone) disk encased in immune-regulating hydrogels for enhanced bone regenerationYou Wu0Xiaokun Yue1Ying Zhang2Ning Yu3Chengyan Ge4Rui Liu5Zhongying Duan6Lilong Gao7Xinlong Zang8Xin Sun9Deteng Zhang10China Uruguay Bio-Nano Pharmaceutical Joint Laboratory, Institute of Neuroregeneration and Neurorehabilitation, Qingdao University, 308 Ningxia Road, Qingdao, 266071, Shandong, China; Qingdao Medical College, Qingdao University, Qingdao, 266071, ChinaShanghai Key Laboratory of Orthopaedic Implants, Department of Orthopaedic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, No. 639 Zhizaoju Road, Shanghai, 200011, ChinaChina Uruguay Bio-Nano Pharmaceutical Joint Laboratory, Institute of Neuroregeneration and Neurorehabilitation, Qingdao University, 308 Ningxia Road, Qingdao, 266071, Shandong, China; Qingdao Medical College, Qingdao University, Qingdao, 266071, ChinaDepartment of Anesthesiology, Affiliated Hospital of Qingdao University, No. 16 Jiangsu Road, Qingdao, 266000, Shandong, ChinaDepartment of Neurosurgery, Affiliated Hospital of Qingdao University, No. 16 Jiangsu Road, Qingdao, 266000, Shandong, ChinaChina Uruguay Bio-Nano Pharmaceutical Joint Laboratory, Institute of Neuroregeneration and Neurorehabilitation, Qingdao University, 308 Ningxia Road, Qingdao, 266071, Shandong, China; Qingdao Medical College, Qingdao University, Qingdao, 266071, ChinaChina Uruguay Bio-Nano Pharmaceutical Joint Laboratory, Institute of Neuroregeneration and Neurorehabilitation, Qingdao University, 308 Ningxia Road, Qingdao, 266071, Shandong, China; Qingdao Medical College, Qingdao University, Qingdao, 266071, ChinaSchool of Materials Science and Engineering, Qingdao University, Qingdao, 266071, ChinaQingdao Medical College, Qingdao University, Qingdao, 266071, ChinaDepartment of Orthopaedics, Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, No. 600 Yishan Road, Shanghai, 200233, China; Corresponding author.China Uruguay Bio-Nano Pharmaceutical Joint Laboratory, Institute of Neuroregeneration and Neurorehabilitation, Qingdao University, 308 Ningxia Road, Qingdao, 266071, Shandong, China; Qingdao Medical College, Qingdao University, Qingdao, 266071, China; Corresponding author. China Uruguay Bio-Nano Pharmaceutical Joint Laboratory, Institute of Neuroregeneration and Neurorehabilitation, Qingdao University, 308 Ningxia Road, Qingdao, 266071, Shandong, China.Well-designed artificial scaffolds are urgently needed due to the limited self-repair capacity of bone, which hampers effective regeneration in critical defects. Optimal scaffolds must provide physical guidance to recruit cells and immune regulation to improve the regenerative microenvironment. This study presents a novel scaffold composed of dual-sided centripetal microgrooved poly(D,L-lactide-co-caprolactone) (PLCL) film combined with a dynamic hydrogel containing prednisolone (PLS)-loaded Prussian blue nanoparticles (PB@PLS). The microgrooves on the surface of the PLCL film were imprinted using a micropatterned polydimethylsiloxane (PDMS) template. Following aminolysis, the PLCL film was covalently grafted with the EM-7 peptide via glutaraldehyde. Functional group analysis, surface morphology and hydrophilicity were evaluated using X-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM), and an optical contact angle measuring instrument, respectively. Bone regeneration-related cells (e.g., bone marrow mesenchymal stem cells, macrophages, Schwann cells, and endothelial cells) cultured on PLCL films tended to align along the stripes and migrate from the periphery toward the center region in vitro. Subsequently, the PLCL film was encapsulated in an immune-regulating hydrogel synthesized from thiol-modified gelatin and Cu2+ in the presence of PB@PLS nanoparticles, which demonstrated excellent antioxidant properties. This scaffold significantly accelerated critical-sized bone regeneration, as evidenced by an increase in the volume of newly formed bone and histological images in vivo. This innovative approach holds substantial promise for clinical applications in bone regeneration and broader tissue repair.http://www.sciencedirect.com/science/article/pii/S2590006424004976Centripetal microgroovesImmune regulationHydrogelsPrussian blue nanoparticlesBone regeneration |
| spellingShingle | You Wu Xiaokun Yue Ying Zhang Ning Yu Chengyan Ge Rui Liu Zhongying Duan Lilong Gao Xinlong Zang Xin Sun Deteng Zhang Dual-sided centripetal microgrooved poly (D,L-lactide-co-caprolactone) disk encased in immune-regulating hydrogels for enhanced bone regeneration Materials Today Bio Centripetal microgrooves Immune regulation Hydrogels Prussian blue nanoparticles Bone regeneration |
| title | Dual-sided centripetal microgrooved poly (D,L-lactide-co-caprolactone) disk encased in immune-regulating hydrogels for enhanced bone regeneration |
| title_full | Dual-sided centripetal microgrooved poly (D,L-lactide-co-caprolactone) disk encased in immune-regulating hydrogels for enhanced bone regeneration |
| title_fullStr | Dual-sided centripetal microgrooved poly (D,L-lactide-co-caprolactone) disk encased in immune-regulating hydrogels for enhanced bone regeneration |
| title_full_unstemmed | Dual-sided centripetal microgrooved poly (D,L-lactide-co-caprolactone) disk encased in immune-regulating hydrogels for enhanced bone regeneration |
| title_short | Dual-sided centripetal microgrooved poly (D,L-lactide-co-caprolactone) disk encased in immune-regulating hydrogels for enhanced bone regeneration |
| title_sort | dual sided centripetal microgrooved poly d l lactide co caprolactone disk encased in immune regulating hydrogels for enhanced bone regeneration |
| topic | Centripetal microgrooves Immune regulation Hydrogels Prussian blue nanoparticles Bone regeneration |
| url | http://www.sciencedirect.com/science/article/pii/S2590006424004976 |
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