激活Nrf2-ARE通路改善尿毒症患者血清诱导的内皮细胞功能障碍及其机制
【目的】探讨激活核因子E2相关因子2（Nrf2）-抗氧化反应元件（ARE）通路改善尿毒症患者血清诱导的内皮细胞功能障碍的作用及其机制。【方法】体外培养人主动脉内皮细胞，分别与10%正常人血清、10%非糖尿病尿毒症患者血清及10%糖尿病尿毒症患者血清共培养，同时用20 μmol/L叔丁基对苯二酚（tBHQ）预处理人主动脉内皮细胞4 h，实验分为6 组：正常人血清组、非糖尿病尿毒症患者血清组、糖尿病尿毒症患者血清组、正常人血清+tBHQ组、非糖尿病尿毒症患者血清+tBHQ组、糖尿病尿毒症患者血清+tBHQ组。流式细胞学技术检测细胞凋亡率、一氧化氮（NO）含量；Western blotting检测内...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | zho |
| Published: |
Editorial Office of Journal of Sun Yat-sen University
2017-01-01
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| Series: | Zhongshan Daxue xuebao. Yixue kexue ban |
| Subjects: | |
| Online Access: | http://xuebaoyx.sysu.edu.cn/zh/article/43568889/ |
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