An Injectable IPN Nanocomposite Hydrogel Embedding Nano Silica for Tissue Engineering Application
Abstract Gelatin methacrylate (GM) and sodium alginate (SA) are two biomaterials that have been widely employed in tissue engineering, particularly in 3D bioprinting. However, they have some drawbacks including undesirable physico‐mechanical properties and printability, hindering their application....
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| Format: | Article |
| Language: | English |
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Wiley-VCH
2025-01-01
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| Series: | Macromolecular Materials and Engineering |
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| Online Access: | https://doi.org/10.1002/mame.202400242 |
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| author | Ali Kakapour Saied Nouri Khorasani Shahla Khalili Mahshid Hafezi Mehdi Sattari‐Najafabadi Mahsa Najarzadegan Samin Saleki Shadab Bagheri‐Khoulenjani |
| author_facet | Ali Kakapour Saied Nouri Khorasani Shahla Khalili Mahshid Hafezi Mehdi Sattari‐Najafabadi Mahsa Najarzadegan Samin Saleki Shadab Bagheri‐Khoulenjani |
| author_sort | Ali Kakapour |
| collection | DOAJ |
| description | Abstract Gelatin methacrylate (GM) and sodium alginate (SA) are two biomaterials that have been widely employed in tissue engineering, particularly in 3D bioprinting. However, they have some drawbacks including undesirable physico‐mechanical properties and printability, hindering their application. This work developed an interpenetrating polymeric network (IPN) of GM and SA reinforced with silica nanoparticles (SNPs) to deal with hydrogels’ drawbacks. Besides, for cross‐linking, visible light is used as an alternative to UV light to prevent disruptions in cellular metabolism and immune system reactions. Four GM/SA/SNP hydrogels different in SNPs concentration (0, 0.5, 1, and 2 w/w%) are studied. The performance of the hydrogels is evaluated in terms of physico‐mechanical properties (viscoelasticity, compressive modulus, degradation, and swelling), rheological properties, and biological properties (fibroblast cell growth and adhesion, and MTT assay). The results demonstrated that the GM/SA/SNP hydrogel with 1% SNPs provided desirable physical (645% swelling and 59.3% degradation), mechanical strength (270 kPa), rheological (tan δ of almost 0.14), and biological performances (≈98% viability after 3 days) while maintaining appropriate printability. The findings suggest that the GM/SA/SNP hydrogel holds great potential for applications in soft tissue regeneration. |
| format | Article |
| id | doaj-art-062ad0efabcc4cdb8b46fad5bd873fcd |
| institution | Kabale University |
| issn | 1438-7492 1439-2054 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Wiley-VCH |
| record_format | Article |
| series | Macromolecular Materials and Engineering |
| spelling | doaj-art-062ad0efabcc4cdb8b46fad5bd873fcd2025-01-13T15:24:25ZengWiley-VCHMacromolecular Materials and Engineering1438-74921439-20542025-01-013101n/an/a10.1002/mame.202400242An Injectable IPN Nanocomposite Hydrogel Embedding Nano Silica for Tissue Engineering ApplicationAli Kakapour0Saied Nouri Khorasani1Shahla Khalili2Mahshid Hafezi3Mehdi Sattari‐Najafabadi4Mahsa Najarzadegan5Samin Saleki6Shadab Bagheri‐Khoulenjani7Department of Chemical Engineering Isfahan University of Technology Isfahan 84156‐83111 IranDepartment of Chemical Engineering Isfahan University of Technology Isfahan 84156‐83111 IranDepartment of Chemical Engineering Isfahan University of Technology Isfahan 84156‐83111 IranDepartment of Chemical Engineering Isfahan University of Technology Isfahan 84156‐83111 IranDepartment of Chemical Engineering Isfahan University of Technology Isfahan 84156‐83111 IranDepartment of Chemical Engineering Isfahan University of Technology Isfahan 84156‐83111 IranDepartment of Chemical Engineering Isfahan University of Technology Isfahan 84156‐83111 IranPolymer and Color Engineering Department Amirkabir University of Technology Tehran 15875‐4413 IranAbstract Gelatin methacrylate (GM) and sodium alginate (SA) are two biomaterials that have been widely employed in tissue engineering, particularly in 3D bioprinting. However, they have some drawbacks including undesirable physico‐mechanical properties and printability, hindering their application. This work developed an interpenetrating polymeric network (IPN) of GM and SA reinforced with silica nanoparticles (SNPs) to deal with hydrogels’ drawbacks. Besides, for cross‐linking, visible light is used as an alternative to UV light to prevent disruptions in cellular metabolism and immune system reactions. Four GM/SA/SNP hydrogels different in SNPs concentration (0, 0.5, 1, and 2 w/w%) are studied. The performance of the hydrogels is evaluated in terms of physico‐mechanical properties (viscoelasticity, compressive modulus, degradation, and swelling), rheological properties, and biological properties (fibroblast cell growth and adhesion, and MTT assay). The results demonstrated that the GM/SA/SNP hydrogel with 1% SNPs provided desirable physical (645% swelling and 59.3% degradation), mechanical strength (270 kPa), rheological (tan δ of almost 0.14), and biological performances (≈98% viability after 3 days) while maintaining appropriate printability. The findings suggest that the GM/SA/SNP hydrogel holds great potential for applications in soft tissue regeneration.https://doi.org/10.1002/mame.202400242gelatin methacrylateinterpenetrated polymer networks (IPNs)printable hydrogelsilica nanoparticlesodium alginate |
| spellingShingle | Ali Kakapour Saied Nouri Khorasani Shahla Khalili Mahshid Hafezi Mehdi Sattari‐Najafabadi Mahsa Najarzadegan Samin Saleki Shadab Bagheri‐Khoulenjani An Injectable IPN Nanocomposite Hydrogel Embedding Nano Silica for Tissue Engineering Application Macromolecular Materials and Engineering gelatin methacrylate interpenetrated polymer networks (IPNs) printable hydrogel silica nanoparticle sodium alginate |
| title | An Injectable IPN Nanocomposite Hydrogel Embedding Nano Silica for Tissue Engineering Application |
| title_full | An Injectable IPN Nanocomposite Hydrogel Embedding Nano Silica for Tissue Engineering Application |
| title_fullStr | An Injectable IPN Nanocomposite Hydrogel Embedding Nano Silica for Tissue Engineering Application |
| title_full_unstemmed | An Injectable IPN Nanocomposite Hydrogel Embedding Nano Silica for Tissue Engineering Application |
| title_short | An Injectable IPN Nanocomposite Hydrogel Embedding Nano Silica for Tissue Engineering Application |
| title_sort | injectable ipn nanocomposite hydrogel embedding nano silica for tissue engineering application |
| topic | gelatin methacrylate interpenetrated polymer networks (IPNs) printable hydrogel silica nanoparticle sodium alginate |
| url | https://doi.org/10.1002/mame.202400242 |
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