The Association Between Pro-Inflammatory Cytokines and Antiretroviral Therapy Resistance-Related Mutations in HIVPositive Patients
Objective: Human-immunodeficiency virus (HIV) infection initiates the dysregulated production of pro-inflammatory cytokines. Moreover, unsuccessful treatment for HIV might be due to the drug resistance mutations (DRM) against anti-retroviral therapy (ART). This study aimed to compare pro-inflammator...
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| Format: | Article |
| Language: | English |
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Galenos Publishing House
2024-08-01
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| Series: | Turkish Journal of Immunology |
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| Online Access: | https://jag.journalagent.com/z4/download_fulltext.asp?pdir=tji&un=TJI-64935 |
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| _version_ | 1841525592417107968 |
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| author | Dwitya Elvira Rizka Nadia Raveinal Raveinal |
| author_facet | Dwitya Elvira Rizka Nadia Raveinal Raveinal |
| author_sort | Dwitya Elvira |
| collection | DOAJ |
| description | Objective: Human-immunodeficiency virus (HIV) infection initiates the dysregulated production of pro-inflammatory cytokines. Moreover, unsuccessful treatment for HIV might be due to the drug resistance mutations (DRM) against anti-retroviral therapy (ART). This study aimed to compare pro-inflammatory cytokines levels of HIV patients, who received first line ART and patients, with mutations of M184V and K103N.
Materials and Methods: This was a case-control study which involved eighty patients with AIDS who received first-line anti-retroviral therapy for at least 6 months. The respondents were divided into two groups of patients with HIV with DRM and the ones with HIV without DRM. M184V and K103N drug resistance mutations to ART were analyzed using reverse transcription-polymerase chain reaction (RT-PCR) meanwhile tumour necrosis factor alpha (TNF-α) and interleukin (IL)-6 as pro-inflammatory cytokines were measured using enzyme linked immunosorbent assay.
Results: Most of the patients were men with an average age of 35.5 +- 9.2 years. The median levels for TNF-α and IL-6 were found 43.3 (4.3-96.1) pg/mL and 46.2 (15.5-158.1) pg/mL, respectively. The results showed that the DRM group got higher values compared to the non-DRM group, but there was no statistically significant difference found between both groups.
Conclusion: There were no differences in pro-inflammatory cytokines between the DRM AIDS patients group compared to the non-DRM who received first-line ART with mutations of M184V and K103N. |
| format | Article |
| id | doaj-art-03c802c4810a45eaa85d18bef5b95fa8 |
| institution | Kabale University |
| issn | 1301-109X 2147-8325 |
| language | English |
| publishDate | 2024-08-01 |
| publisher | Galenos Publishing House |
| record_format | Article |
| series | Turkish Journal of Immunology |
| spelling | doaj-art-03c802c4810a45eaa85d18bef5b95fa82025-01-17T08:19:14ZengGalenos Publishing HouseTurkish Journal of Immunology1301-109X2147-83252024-08-01122657010.4274/tji.galenos.2024.64935TJI-64935The Association Between Pro-Inflammatory Cytokines and Antiretroviral Therapy Resistance-Related Mutations in HIVPositive PatientsDwitya Elvira0Rizka Nadia1Raveinal Raveinal2Andalas University Medical Faculty, Dr. M. Djamil General Hospital, Clinic of Internal Medicine, Allergy and Immunology Subdivision, Padang, IndonesiaAndalas University Medical Faculty, Dr. M. Djamil General Hospital, Clinic of Internal Medicine, Padang, IndonesiaAndalas University Medical Faculty, Dr. M. Djamil General Hospital, Clinic of Internal Medicine, Allergy and Immunology Subdivision, Padang, IndonesiaObjective: Human-immunodeficiency virus (HIV) infection initiates the dysregulated production of pro-inflammatory cytokines. Moreover, unsuccessful treatment for HIV might be due to the drug resistance mutations (DRM) against anti-retroviral therapy (ART). This study aimed to compare pro-inflammatory cytokines levels of HIV patients, who received first line ART and patients, with mutations of M184V and K103N. Materials and Methods: This was a case-control study which involved eighty patients with AIDS who received first-line anti-retroviral therapy for at least 6 months. The respondents were divided into two groups of patients with HIV with DRM and the ones with HIV without DRM. M184V and K103N drug resistance mutations to ART were analyzed using reverse transcription-polymerase chain reaction (RT-PCR) meanwhile tumour necrosis factor alpha (TNF-α) and interleukin (IL)-6 as pro-inflammatory cytokines were measured using enzyme linked immunosorbent assay. Results: Most of the patients were men with an average age of 35.5 +- 9.2 years. The median levels for TNF-α and IL-6 were found 43.3 (4.3-96.1) pg/mL and 46.2 (15.5-158.1) pg/mL, respectively. The results showed that the DRM group got higher values compared to the non-DRM group, but there was no statistically significant difference found between both groups. Conclusion: There were no differences in pro-inflammatory cytokines between the DRM AIDS patients group compared to the non-DRM who received first-line ART with mutations of M184V and K103N.https://jag.journalagent.com/z4/download_fulltext.asp?pdir=tji&un=TJI-64935cytokinedrug resistancehiv/aids patientil-6mutationtnf-α |
| spellingShingle | Dwitya Elvira Rizka Nadia Raveinal Raveinal The Association Between Pro-Inflammatory Cytokines and Antiretroviral Therapy Resistance-Related Mutations in HIVPositive Patients Turkish Journal of Immunology cytokine drug resistance hiv/aids patient il-6 mutation tnf-α |
| title | The Association Between Pro-Inflammatory Cytokines and Antiretroviral Therapy Resistance-Related Mutations in HIVPositive Patients |
| title_full | The Association Between Pro-Inflammatory Cytokines and Antiretroviral Therapy Resistance-Related Mutations in HIVPositive Patients |
| title_fullStr | The Association Between Pro-Inflammatory Cytokines and Antiretroviral Therapy Resistance-Related Mutations in HIVPositive Patients |
| title_full_unstemmed | The Association Between Pro-Inflammatory Cytokines and Antiretroviral Therapy Resistance-Related Mutations in HIVPositive Patients |
| title_short | The Association Between Pro-Inflammatory Cytokines and Antiretroviral Therapy Resistance-Related Mutations in HIVPositive Patients |
| title_sort | association between pro inflammatory cytokines and antiretroviral therapy resistance related mutations in hivpositive patients |
| topic | cytokine drug resistance hiv/aids patient il-6 mutation tnf-α |
| url | https://jag.journalagent.com/z4/download_fulltext.asp?pdir=tji&un=TJI-64935 |
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