Implementation of the quality-by-design concept for an adeno-associated viral vector-based gene therapy by D. S. Kopein, G. N. Poroshin, R. A. Khamitov

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In vivo production of CAR T cell: Opportunities and challenges by Zhiqiang Song, Yi Zhou, Binbin Wang, Yuke Geng, Gusheng Tang, Yang Wang, Jianmin Yang

“…Chimeric antigen receptor T (CAR T) cell therapy has achieved remarkable efficacy for patients with hematological malignancies. However, in vitro viral vector-mediated production of CAR T cells is time-consuming and expensive and impairs T cell function. …”
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serologic markers of Epstein-Barr virus reactivation in the conditions of viral encephalitis in young patients by O. V. Goleva, E. A. Murina, Z. A. Osipova

“…The algorithm of the study included serological methods on the basis of the enzymelinked immunosorbent assay manufactured by Joint-Stock Company «Vector-Best» (Novosibirsk) on the open-type apparatus «Lasurit» of «Dynex Technologies Inc.» company (USA) and blot-technologies with the use of Western-blotting manufactured by Euroimmun AG, Germany on the apparatus «AUTOBLOTT 3000» of «MedTec, Inc.» company (USA). …”
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Generalized Varicella Zoster infection in a patient with lymphogranulomatosis after bone marrow transplantation: clinical and laboratory characteristics and virus genotyping by N. V. Matsiyeuskaya, E. O. Samoilovich, G. V. Semeyko, O. V. Gvozdelyuk, A. S. Yushkevich

“…A description of a laboratory confirmed clinical case of generalized Varicella Zoster infection in a patient with lymphogranulomatosis after bone marrow transplantation is presented.Detection of the Varicella Zoster virus in biological material was carried out by real-time PCR using the RealBest DNA VZV test system manufactured by Vector-BEST, Russian Federation. Isolation of viral DNA from biological material was performed using the ArtRNA MiniSpin kit (ArtBioTech, Belarus) or QIAamp DNA mini kit (QIAGEN, Germany).The disease had an atypically severe course with a multiorgan lesion, represented not only by lesions  of  the  skin and mucous membranes, but also by the development of encephalitis, myocarditis, and pneumonia. …”
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