Mechanism study of KCMF1 promoting proliferation and NF-κB signaling transduction in colorectal cancer cells by WU Zhibai, XU Guiqin, ZHANG Li, YANG Zhaojuan, LIU Yun, JIAO Kun, CHEN Zehong, XU Chen, ZUO You, ZHENG Ningqian, YE Zhiqian, LIU Yongzhong

“…KCMF1 expression level was negatively correlated with the survival time of patients with CRC (P<0.01), and was positively associated with CRC clinical stage (P<0.05). …”
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Supplementation of Nodulisporium sp. KT29 induced by Vibrio harveyi as an immunostimulant for controlling vibriosis in vannamei white shrimp under marine culture system by Dinamella Wahjuningrum, Teuku Reza Efianda, Kustiariyah Tarman, Munti Yuhana, Irzal Effendi, Fazril Saputra

“…KT29 and infected with V. harveyi), KN (negative controls without Nodulisporium sp. KT29 and infected with physiological solution), NT (treatment of supplementation Nodulisporium sp KT29 20 mL/kg and infected with V. harveyi), NM (treatment of supplementation test Nodulisporium sp. …”
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Development of a real-time duplex PCR assay for simultaneously diagnosis of KHV and CEV in common carp and koi by Khumaira Puspasari, Adityawati Fajar Rini, Noviana Dewi, Ratih Ismayasari

“…For clinical validation, 18 KHV and CEV positive samples each, as well as 12 negative samples were tested with three different test methods, i.e., real-time duplex PCR, real-time simplex PCR, and conventional PCR. …”
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Im Niemandsland zwischen strengem Satz und Historismus. Zur Krise der Kontrapunktlehre im mittleren 19. Jahrhundert by Florian Edler

“…This essay explores to what extent these negative connotations originated in the problems and inner contradictions in contemporary counterpoint teaching. …”
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The Contribution of the Hotel Industry on Youth Empowerment in Mbarara City: A Case Study of Hotel Triangle Mbarara City. by Owomuhangi, Emmanuel

“…From the field, 90% agree that Low pay negatively impacts job satisfaction and motivation. …”
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Social Confrontation with Menstruation: A Feminist Ethnography of Menstrual Experience among Female Instagram Users by Rana Mohammad Taghinejad Esfahani, Majid Movahed, Halimeh Enayat, Aliyar Ahmadi

“…These efforts aim to shift negative attitudes and enhance public awareness on the subject. …”
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Insights from a Pilot Study of Naloxone Education

“…Conclusion: Introducing a Naloxone component to first aid education targeting potential witnesses of an opioid overdose situation does not seem to negatively impact on learners’ confidence or willingness to act, though the methodological challenges of this study limits the conclusiveness of this statement. …”
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Investigating the mediating role of academic self-sufficiency and basic psychological needs in the relationship of social skills and social intelligence with academic achievement by Fatimeh Sabih Mahdi, Hasan ghalavandi

“…Academic decline is the negative aspect of it; one of the problems of the educational system, which manifests itself in various ways such as the failure of students to achieve the goals of their respective educational levels, failure and repetition of educational levels, early school leaving, unemployment and indecision (Khazaei, 2016). …”
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Regulatory role of lnc-MAP3K13-3:1 on miR-6894-3p and SHROOM2 in modulating cellular dynamics in hepatocellular carcinoma by Kuai Chen, Manqin Zhu, Qinghua Hu, Hui Huang, Ka Chen, Xia Shuai, Jinshi Huang, Qiang Tao, Zhibin Guo

“…Stable cell lines overexpressing lnc-MAP3K13-3:1 were developed, and cells were transfected with miR-6894-3p mimics or a mimic negative control (NC). After 24 h, qPCR was utilized to quantify the relative expression of lnc-MAP3K13-3:1, miR-6894-3p, SHROOM2, and Caspase9 mRNA in each group. …”
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Mechanism of Inhibition of Inflammation in Osteoarthritis by Tougu Xiaotong Capsule via Regulating Ras-Raf-MEK1/2-ERK1/2 Signaling Pathway by Munan LIN, Xiang SHAO, Limei XU, Xin ZHANG, Zaishi ZHU, Yidan HAN, Xinwei DUAN, Jianmei CHEN, song GAO, Xihai LI

“…Isolated chondrocytes of passage two were divided into blank group, model group(treatment with 10 ng/mL of LPS for 8 h), inhibitor group(treatment with 10 ng/mL of U0126 for 30 min followed by 10 ng/mL of LPS for additional 8 h)and TXC group(treatment with 10 ng/mL of LPS plus 300 μg/mL of TXC for 8 h).Western blot was carried out to detect the expression of Ras, Raf, p-ERK1/2, MEK1/2, MMP-3 and MMP-13.qPCR was used to detect the mRNAs expression of MEK1/2, ERK1/2, MMP-3 and MMP-13, as well as the expression of miR-27b, miR-34a and miR-146a.Results:1)The cytoplasm of isolated cells(P2)from the cartilage of knee joint of 4-week-old SD male rats was stained brown by typeⅡcollagen immunohistochemical staining as compared with the negative control cells, which indicated that the extracted cells were chondrocytes.2)The expression of Ras, Raf, MEK1/2, MMP-3, MMP-13 in the model group was significantly higher than that in the blank group, respectively(<italic>P</italic>&lt;0.01, <italic>P</italic>&lt;0.05), but the expression of p-ERK1/2 had no significant difference(<italic>P</italic>&gt;0.05);the expression of Ras, Raf, p-ERK1/2, MEK1/2, MMP-3, MMP-13 in both the inhibitor group and the TXC group was lower than that in the model group, respectively(<italic>P</italic>&lt;0.01, <italic>P</italic>&lt;0.05), but the expression of MEK1/2 in the TXC group had no significant difference compared to that in the model group(<italic>P</italic>&gt;0.05).3)The mRNA expression of MEK1/2, ERK1/2, MMP-3 and MMP-13 in the model group was significantly higher than that in the blank group, respectively(<italic>P</italic>&lt;0.01);their expression in both the inhibitor group and the TXC group was lower than that in the model group, respectively(<italic>P</italic>&lt;0.01, <italic>P</italic>&lt;0.05).4)The expression of miR-27b in the model group was significantly lower than that in the blank group(<italic>P</italic>&lt;0.01), but its expre-ssion in both the inhibitor group and the TXC group was higher than that in the model group(<italic>P</italic>&lt;0.01);the expression of both miR-34a and miR-146a in the model group was significantly higher than that in the blank group, respectively(<italic>P</italic>&lt;0.01, <italic>P</italic>&lt;0.05);however, their expression in both the inhibitor group and the TXC group was lower than that in the model group, respectively(<italic>P</italic>&lt;0.01, <italic>P</italic>&lt;0.05).Conclusion:Tougu Xiaotong capsule regulates the expression of key factors in Ras-Raf-MEK1/2-ERK1/2 signaling pathway and miR-27b, miR-34a and miR-146a to regulate the inflammatory of chondrocytes, thereby inhibiting osteoarthritis inflammation and protecting articular cartilage.…”
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Potential of long-term satellite observations and reanalysis products for characterising soil drying: trends and drought events by M. Hirschi, P. Stradiotti, B. Crezee, B. Crezee, W. Dorigo, S. I. Seneviratne

“…The differences between reanalysis products are related to a positive mean bias in the precipitation trends and regionally negative biases in surface air temperature trends in MERRA-2 compared with ground observational products, suggesting that this reanalysis underestimates drying trends. …”
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A qualitative study of symptoms and psychological experience of patients with delayed resolution after microvascular decompression for hemifacial spasm (面肌痉挛行微血管减压术后延迟治愈患者症状体验和心理感受... by JING Dan (井丹), LYU Yan (吕燕), XU Zai (徐再)

“…Results There are four main themes: Treatment of disease and cognition; Psychological issues such as excessive negative expectation, expectation bias, autosuggestion of surgery failure and positive coping style; Physical issuers such as headache, nausea and vomiting dizziness; incomprehension and support of social relations. …”
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Pengaruh Pemberian Aspartam terhadap Kadar Glukosa Darah Tikus Diabetes Melitus Diinduksi Aloksan by Ega Purnamasari R.D, Eti Yerizel, Efrida .

“…Twenty male Wistar rats were divided into 4 groups: negative control (KN), positive control (KP), treatment 1 (P1), treatment 2 (P2). …”
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Dual inhibition of LAG-3 and PD-1 with IBI110 and sintilimab in advanced solid tumors: the first-in-human phase Ia/Ib study by Chenyu Mao, Anwen Xiong, Jiong Qian, Wenxiang Wang, Ying Liu, Tao Zhang, Zhihai Wu, Haiqing Ni, Jia Lu, Sixiang Long, Li Zhao, Yuling Chen, Caicun Zhou, Nong Xu

“…Additionally, phase Ib combination dose expansion of IBI110 plus sintilimab and chemotherapy was conducted in previously untreated, advanced squamous non-small cell lung cancer (sqNSCLC) and HER-2 negative gastric cancer (GC). In phase Ia dose escalation, patients received IBI110 monotherapy at 0.01/0.1/0.3/1/3/10/20 mg/kg Q3W. …”
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Attitudes Toward Psychotherapeutic Treatment and Health Literacy in a Large Sample of the General Population in Germany: Cross-Sectional Study by Rebekka Schröder, Tim Hamer, Ralf Suhr, Lars König

“…An important factor preventing individuals from seeking professional help is negative attitudes toward psychotherapeutic treatment. …”
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Kappa-carrageenan as immunostimulant to control infectious myonecrosis (IMN) disease in white shrimp Litopenaeus vannamei by Dian Febriani, . Sukenda, Sri Nuryati

“…Both stages had positive and negative control and performed in complete randomized design. …”
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Optimasi Convolutional Neural Network Untuk Deteksi Covid-19 pada X-ray Thorax Berbasis Dropout by I Gede Totok Suryawan, I Putu Agus Eka Darma Udayana

“…It proved that when this system was tested with COVID-19 thorax x-ray public database data, the classifier that used this architecture could achieve an accuracy rate of 95.20% followed by precision and recall value reaching 94.80% and 94.80%. respectively and last but not least F-score of 95.18% and Negative Predictive value of 95.60%  It could also save training time up to 62% compared to other deep learning architectures. …”
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A retrospective study of pembrolizumab combined with XELOX regimen in the treatment of advanced gastric cancer by FENG Huizhi, LIU Jingmei, BU Xiaoqian

“…Inclusion criteria: patients with HER2-negative, untreated advanced gastric cancer or adenocarcinoma of the esophagogastric junction, confirmed by clinical histopathological examination and meeting relevant diagnostic criteria; TNM stage Ⅳ; age ≥20 years; expected survival ≥6 months; no severe organ damage before treatment; Karnofsky score &gt;60; patients with complete clinical data. …”
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Prognostic value of intraductal carcinoma subtypes and postoperative radiotherapy for localized prostate cancer by Fang Cao, Qing Li, Tianyu Xiong, Yingjie Zheng, Tian Zhang, Mulan Jin, Liming Song, Nianzeng Xing, Yinong Niu

“…While postoperative radiotherapy can alleviate the negative prognostic impact from IDC-P. It is implied that IDC-P can also be an indicator to be considered in PORT decision making to some extent.…”
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Whole-Genome Methylation Sequencing Analysis and Functional Verification of LIM-Homeobox Family Genes in Cervical Cancer by Yu R, Yu Q, Shi J, Meng X, Deng Z, Suo J, Yang H

“…LHX5 and LHX9 have a negative effect on cervical cancer. The migration ability of LHX9 silenced cells was significantly enhanced after irradiation.Keywords: cervical cancer, radiation therapy, whole-genome bisulfite sequencing, WGBS, LHX2, LHX5, LHX9…”
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