Testicular biometrics of Murrah buffaloes in different age groups by Erly L. Carrascal-Triana, Jorge Mejía Lúquez, Divier Agudelo Gómez, Edison Julián Ramírez Toro

“…The measurements analyzed were scrotal circumference (SC), spermatic cord circumference (CC), Testicular volume (TV), Testicular length (TL), and width (TW). …”
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GPR87 promotes invasion and migration through the RHO/ROCK pathway in non-small cell lung cancer by LIU Chenxi, HAN Lin, YANG Yi, ZHOU Han, LIU Yayun, SHENG Deqiao

“…ELISA was used to detect the secretion of MMP7 in the culture supernatant. RT-qPCR was used to detect the mRNA expression levels of GPR87, MMP2, MMP7, MMP9, E-cadherin, N-cadherin, vimentin, snail, twist, RHOA, RHOC, and ROCK1. …”
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Warm and humid environment induces gut microbiota dysbiosis and bacterial translocation leading to inflammatory state and promotes proliferation and biofilm formation of certain ba... by Yinrui Guo, Jianlang He, Shaojie Li, Shiqi Zou, Haiting Zhang, Xin Yang, Jian Wang

“…C. albicans co-cultured with RAW264.7 cells increased TNF-α secretion, and the supernatant promoted pathogenic bacterial proliferation and biofilm formation. …”
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Speciation of Potentially Carcinogenic Trace Nickel(II) Ion Levels in Human Saliva: A Sequential Metabolomics-Facilitated High-Field ¹H NMR Investigation by Kayleigh Hunwin, Georgina Page, Mark Edgar, Mohammed Bhogadia, Martin Grootveld

“…<b>Methods:</b> Unstimulated whole-mouth human saliva samples were obtained from n = 12 pre-fasted (≥8 h) healthy participants, and clear whole-mouth salivary supernatants (WMSSs) were obtained from these via centrifugation. …”
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Effect of integrin α5 on NLRP3 expression in periodontal ligament fibroblasts within an inflammatory microenvironment by DAI Jingyi, CAI Hongxuan, SI Weixing, ZHANG Zan, WANG Zhurui, LI Mengsen, TIAN Ya guang

“…After rat PDLFs were treated with LPS (0.5, 5, and 50 &#x00B5;g/mL) for 24 h, the primary medium was discarded and replaced with serum-free culture medium. After 24 h, the supernatant was collected and mixed with DMEM medium containing 10% exosome-free serum at a volume ratio of 1:1 to obtain conditioned medium (CM). …”
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Pien Tze Huang Attenuates Neuro-inflammatory Injury in BV2 Microglial Cells Induced by Lipopolysaccharide via Inhibition of TLR4/MAPK Signaling Pathway by Zhenwei HUANG, Qing ZHANG, Lili HUANG, Xiaoqin ZHANG, Mingqing HUANG

“…The inflammatory response in BV2 microglial cells was induced by LPS(100 ng/mL), while the cells were treated with different concentrations of PZH(0.05, 0.10 and 0.15 mg/mL) for 12 h. Then supernatant was collected and the concentration of IL-6 was detected by enzyme-linked immunosorbent assay (ELISA) kit. …”
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Effects of Density Stress on Growth and Physiology of Epinephelus fuscoguttatus ♀×Epinephelus lanceolatus ♂ by Xianhong ZHANG, Wenyang LI, Baoliang LIU, Fan FEI, Xiaoqiang GAO, Ran GUO, Shuquan CAO, Zhiwen ZHU

“…Anesthesia was administered (MS-222, 80 mg/L), tail vein blood was drawn with a 2 mL disposable syringe, and centrifuged (4 000 r/min, 4 ℃, 10 min) to obtain a supernatant that was stored at –20 ℃ for testing. Before testing the corresponding indicators, the brain, liver, intestine, stomach, and kidney were frozen in liquid nitrogen. …”
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