Examining the impact of probiotic Lactiplantibacillus pentosus 6MMI on inhibiting biofilm formation, adhesion, and virulence gene expression in Listeria monocytogenes ATCC 19115 by Behrooz Alizadeh Behbahani, Mostafa Rahmati-Joneidabad, Morteza Taki

“…Treatment with cell-free supernatant (CFS) from Lpb. pentosus significantly reduced biofilm formation by 91.25 % and 24.50 % and diminished mature biofilm formation by 83.82 % and 21.80 % on L. monocytogenes. …”
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Key bacterial vaginosis-associated bacteria influence each other's growth in biofilms in rich media and media simulating vaginal tract secretions by Lúcia G.V. Sousa, Christina A. Muzny, Nuno Cerca

“…Interestingly, we found that P. bivia growth in NYCIII was influenced by the cell-free supernatant (CFS) of F. vaginae and by the CFS of G. vaginalis in mGTS. …”
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Colonic cytokines for severe ulcerative colitis by Ye. A. Konovich, K. Ye. Shirokikh, I. L. Khalif, M. V. Shapina

“…Cytokine concentration was evaluated in supernatant fluid after centrifugal separation of 10 mg biopsy specimen homogenate by the protein analyzer «Bio-Plex» («Bio-Rad», USA). …”
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In vitro evaluation of the immunomodulatory and antibacterial activities of calcitriol (1,25-dihydroxy-vitamin D3) as a potential application for aerobic vaginitis treatment - prel... by Anna Tomusiak-Plebanek, Grażyna Więcek, Edyta Golińska, Magdalena Strus

“…After 24 h, 48 h, and 72 h post-infection, culture supernatants were collected, and levels of pro-inflammatory cytokines IL-6 and IL-1β were analyzed using enzyme-linked immunosorbent assays (ELISA). …”
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Effect of Stem Cell-Derived Extracellular Vesicles on Damaged Human Corneal Endothelial Cells by Raffaele Nuzzi, Lola Buono, Simona Scalabrin, Marco De Iuliis, Benedetta Bussolati

“…MSC-EVs were obtained from supernatants of MSCs. In order to establish a valid in vitro damage model to test the regenerative potential of EVs on HCECs, we evaluated the proliferation rate and the apoptosis after exposing the cells to serum-deprived medium at different concentrations for 24 hours. …”
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Protective Effects of Two Safflower Derived Compounds, Kaempferol and Hydroxysafflor Yellow A, on Hyperglycaemic Stress-Induced Podocyte Apoptosis via Modulating of Macrophage M1/M... by Yuanping Li, Dan Zheng, Dayue Shen, Xilan Zhang, Xiaoyun Zhao, Hui Liao

“…The expressions of inducible nitric oxide synthase (iNOS), tumor necrosis factor- (TNF-) α, mannose receptor (CD206), and arginase- (Arg-) 1 were quantified by Western blotting and real-time quantitative PCR. The collected supernatants from macrophage were named as (NG) MS, (HG) MS, (Ka) MS, and (HSYA) MS. (2) The podocyte survival rate was assessed by Bromodeoxyuridine assay, while TNF-α and interleukin- (IL-) 1β levels were evaluated by Elisa. …”
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Erbin Regulates Tissue Factors Through Ras/Raf Pathway in Coagulation Disorders in Sepsis by Yang C, Lei C, Jing G, Xia Y, Zhou H, Wu D, Zuo J, Gong H, Wang X, Dong Y, Aidebaike D, Wu X, Song X

“…However, the role of Erbin in coagulation disorders in sepsis and the relationship between Erbin and macrophage regulation of coagulation function are still unclear.Methods: At the cellular level, macrophages were treated with lipopolysaccharide (LPS) or MEK inhibitor (PD98059), protein expression levels were detected by Western blot, co-immunoprecipitation (Co-IP), and immunofluorescence, mRNA expression levels were detected by quantitative real-time polymerase chain reaction (qPCR), and the concentration of tissue factor (TF) in cell supernatant was detected by enzyme linked immunosorbent assay (ELISA). …”
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Cortina d’Ampezzo 1956 tra organizzazione sportiva e relazioni internazionali by Gherardo Bonini, Franco Cervellati, Marcello Marchioni

“…Avvalendosi di fonti archivistiche primarie fino a ora inesplorate, questo elaborato si propone di evidenziare come tale acclamato successo fu veritiero, ma l’organizzazione italiana, nel caso specifico la Segreteria generale dei Giochi che dirigeva in Cortina la preparazione e lo svolgimento dell’evento riuscì a superare difficoltà importanti durante la preparazione e lo svolgimento dei Giochi. …”
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Extracellular vesicles, RNA sequencing, and bioinformatic analyses: Challenges, solutions, and recommendations by Rebecca T. Miceli, Tzu‐Yi Chen, Yohei Nose, Swapnil Tichkule, Briana Brown, John F. Fullard, Marilyn D. Saulsbury, Simon O. Heyliger, Sacha Gnjatic, Natasha Kyprianou, Carlos Cordon‐Cardo, Susmita Sahoo, Emanuela Taioli, Panos Roussos, Gustavo Stolovitzky, Edgar Gonzalez‐Kozlova, Navneet Dogra

“…Here, we address these gaps by describing the following points by (1) focusing on the large canopy of the EVs and particles (EVPs), which includes, but not limited to – exosomes and other large and small EVs, lipoproteins, exomeres/supermeres, mitochondrial‐derived vesicles, RNA binding proteins, and cell‐free DNA/RNA/proteins; (2) examining the potential functional roles and biogenesis of EVPs; (3) discussing various transcriptomic methods and technologies used in uncovering the cargoes of EVPs; (4) presenting a comprehensive list of RNA subtypes reported in EVPs; (5) describing different EV‐RNA databases and resources specific to EV‐RNA species; (6) reviewing established bioinformatics pipelines and novel strategies for reproducible EV transcriptomics analyses; (7) emphasizing the significant need for a gold standard approach in identifying EV‐RNAs across studies; (8) and finally, we highlight current challenges, discuss possible solutions, and present recommendations for robust and reproducible analyses of EVP‐associated small RNAs. …”
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Ruxolitinib synergizes with regulatory T cells to improve inflammation but has no added benefits in decreasing albuminuria in SLE by Mi-Ae Lyu, Ximing Tang, Maria Gabriela Raso, Meixian Huang, Ke Zeng, Tara Sadeghi, Christopher R. Flowers, Simrit Parmar

“…We hypothesize that the addition of ruxolitinib to UCB-Tregs may improve SLE outcomes.MethodsIn vitro cell suppression, phenotype change, IL-10 secretion, and cytokine levels in coculture supernatants were determined to quantify the impact of adding ruxolitinib to UCB-Tregs. …”
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Establishment of the fingerprint of ganoderma lucidum spore powder and study on the anti-inflammatory activity of ganoderma lucidum spore oil by Yu Zhang, Lihui Hou, Le Wang, Yunshan Si, Hongjuan Wen, Ying Gao, Xiuhua Yu

“…[Methods] High Performance Liquid Chromatography and High Performance Thin-layer Chromatography techniques were used to analyze the chemical composition of Ganoderma lucidum spore powder from different origins; MTT method was used to determine the cell viability; ELISA was used to determine the RAW264.7 IL-1β, IL-6 and TNF-α levels in cell supernatant. [Results] The HPLC fingerprints were calibrated with a total of 12 peaks, of which peaks 1-5 were ganoderic acid C2, ganoderic acid G, ganoderic acid B, ganoderic acid A, ganoderic acid D, respectively; the current version of the Pharmacopoeia method of HPTLC showed that 10 batches of samples complied with the regulations, and the quality of raw materials of different origins was found to be similar to that of the HPLC fingerprints after 2 unfoldings by using trichloromethane-acetonitrile-methanol-formic acid (26∶4 ∶1∶1) as the unfolding agent. …”
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Determination of antibiotics in bean sprouts by UHPLC-MS/ MS-internal standard method by ZHANG Xiaoyi, ZHENG Sanyan, ZHANG Xiuyao, LI Ruifen, YANG Shuran

“…ObjectiveTo establish an ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS)-internal standard method for the simultaneous determination of 59 antibiotics, including quinolones, sulfonamides, tetracyclines, macrolides and nitroimidazole in bean sprouts.MethodsThe internal standard compounds and 5.0 mL of acetonitrile containing 0.1% formic acid were added into 5.0 g of the sample, and then extracted by ultrasonication. The extracted supernatant was filtered by 0.22 µm membrane and then injected into 1.0 µL of the sample directly. …”
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iPSC-derived exosomes as amphotericin B carriers: a promising approach to combat cryptococcal meningitis by Jingyu Zhao, Jingyu Zhao, Wei Fang, Yangjie Gao, Jiquan Chen, Guizhen Wang, Julin Gu

“…Amphotericin B (AmB) serves as the cornerstone treatment for CM; however, its clinical application is restricted due to limited penetration of the blood–brain barrier and associated nephrotoxicity.ObjectiveThis study investigates the use of exosomes derived from induced pluripotent stem cells (iPSC-Exos) as carriers for AmB in treating CM, aiming to enhance therapeutic efficacy and safety and reduce AmB toxicity.MethodsExosomes were extracted from iPSC culture supernatants using ultrafiltration and ultracentrifugation. …”
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Virulence factor discovery identifies associations between the Fic gene family and Fap2+ fusobacteria in colorectal cancer microbiomes by Geicho Nakatsu, Duhyun Ko, Monia Michaud, Eric A. Franzosa, Xochitl C. Morgan, Curtis Huttenhower, Wendy S. Garrett

“…Finally, we demonstrate that Fic proteins are synthesized by F. animalis as Fic peptides are detectable in F. animalis monoculture supernatants. Taken together, our study uncovers Fic genes as potential virulence factors in Fap2+ fusobacterial genomes.IMPORTANCEAccumulating data support that bacterial members of the intra-tumoral microbiota critically influence colorectal cancer progression. …”
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Detection Methods for Asparagine and Glutamine Contents in Fruits and Vegetables and Recommendations for Dietary Intake of Fruits and Vegetables in Patients with Urea Cycle Disorde... by YE Xinyu, LI Ziying, TANG Shujun, SUN Liping, ZHAO Yunxia, XIAO Weimin, GU Yaping, KUANG Huiying, CHEN Xin, YANG Guowu, HUANG Yali

“…After enzymatic hydrolysis with Pronase E, samples were treated with butylated hydroxyanisole as an antioxidant. The supernatant was collected for analysis by UPLC-MS/MS. …”
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Distinguishing Compact Objects in Extreme-Mass-Ratio Inspirals by Gravitational Waves by Lujia Xu, Shucheng Yang, Wenbiao Han, Xingyu Zhong, Rundong Tang, Yuanhao Zhang

“…Assuming the “true” GW signals originate from an extended body inspiraling into a supermassive black hole, we compute the signal-to-noise ratio (SNR) and Bayes factors between a test-particle waveform template and our model, which includes the spin and quadrupole of the compact object. …”
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Evaluation of the antibacterial and antibiofilm effect of mycosynthesized silver and selenium nanoparticles and their synergistic effect with antibiotics on nosocomial bacteria by Nahed Fathallah Fahmy, Marwa Mahmoud Abdel-Kareem, Heba A. Ahmed, Mena Zarif Helmy, Ekram Abdel-Rahman Mahmoud

“…Results In the current research, silver nanoparticles (AgNPs) and selenium nanoparticles (SeNPs) were produced extracellularly using A. carneus MAK 259 culture supernatants. Colour change, an initial evaluation of the production of AgNPs and SeNPs. …”
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The Mycoplasma hyopneumoniae protein Mhp274 elicits mucosal and systemic immune responses in mice by Mengqi Xie, Zhongshun Huang, Yun Zhang, Yujie Gan, Huiying Li, Dan Li, Honglei Ding

“…The levels of IFN-γ, IL-4, and IL-17, in the supernatants of splenocytes and in serum were measured, and the mRNA levels of three cytokines in splenocytes were analyzed. …”
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IL-33 Deficiency Attenuates Lung Inflammation by Inducing Th17 Response and Impacting the Th17/Treg Balance in LPS-Induced ARDS Mice via Dendritic Cells by Li Cheng, Yang Jiao, Wei Jiang, Xin Zhang, Liping Zhang, Gongwei Jia

“…The levels of IL-6 in the supernatant in a dendritic cell culture system were examined by ELISA. …”
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Th2-Immune Polarizing and Anti-Inflammatory Properties of Insulin Are Not Effective in Type 2 Diabetic Pregnancy by Adnette Fagninou, Magloire Pandoua Nekoua, Darius Sossou, Kabirou Moutairou, Nadine Fievet, Akadiri Yessoufou

“…The proliferative capacity of T cells was performed, and serum and cell culture supernatant cytokine levels were also quantified. Results. …”
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