Random Mutagenesis of the Aspergillus oryzae Genome Results in Fungal Antibacterial Activity by Cory A. Leonard, Stacy D. Brown, J. Russell Hayman

“…Over 3000 EMS-treated A. oryzae cultures were tested in the screen, and one isolate, CAL220, exhibited altered morphology and antibacterial activity. Culture supernatant from this isolate showed antibacterial activity against Methicillin-sensitive Staphylococcus aureus, MRSA, and Pseudomonas aeruginosa, but not Klebsiella pneumonia or Proteus vulgaris. …”
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Vaccination with Enzymatically Cleaved GPI-Anchored Proteins from Schistosoma mansoni Induces Protection against Challenge Infection by Vicente P. Martins, Carina S. Pinheiro, Barbara C. P. Figueiredo, Natan R. G. Assis, Suellen B. Morais, Marcelo V. Caliari, Vasco Azevedo, William Castro-Borges, R. Alan Wilson, Sergio C. Oliveira

“…Immunization of mice with GPI-anchored proteins induced a mixed Th1/Th2 type of immune response with production of IFN-γ and TNF-α, and low levels of IL-5 into the supernatant of splenocyte cultures. The protection engendered by this vaccination protocol was confirmed by 42% reduction in worm burden, 45% reduction in eggs per gram of hepatic tissue, 29% reduction in the number of granulomas per area, and 53% reduction in the granuloma fibrosis. …”
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Decreased Expression of CD14 in MSU-Mediated Inflammation May Be Associated with Spontaneous Remission of Acute Gout by Lihua Duan, Jiao Luo, Qiang Fu, Ke Shang, Yingying Wei, Youlian Wang, Yan Li, Jie Chen

“…The results showed that CD14 expression on macrophage and sCD14 levels in the culture supernatants were significantly decreased after MSU treatment. …”
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Protective and immunomodulatory effects of the novel probiotic yeast Pichia kudriavzevii isolated from a home-made kefir during infection in human colon epithelial cells: An explor... by Angela Maione, Marianna Imparato, Annalisa Buonanno, Marilena Galdiero, Elisabetta de Alteriis, Marco Guida, Emilia Galdiero

“…In this study we aimed to explore the protective effects of a probiotic yeast Pichia kudriavzevii (Y1) and its cell-free supernatant (CFS-Y1) during infection simulated in vitro using Caco-2 cells. …”
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Moisture Susceptibility of Warm Mix Asphalt (WMA) with an Organic Wax Additive Based on X-Ray Computed Tomography (CT) Technology by Jie Ji, Hui Yao, Zhikai Yuan, Zhi Suo, Ying Xu, Pengfei Li, Zhanping You

“…The warm mix asphalt was fabricated with different moisture contents (0%, 1%, 2%, and 3%) of limestone aggregates using the Superpave gyratory compactor. The moisture susceptibility of asphalt mixtures with an organic wax additive RH was studied. …”
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Effect of In Vitro Exposure of Corticosteroid Drugs, Conventionally Used in AMD Treatment, on Mesenchymal Stem Cells by Raffaele Nuzzi, Monica Gunetti, Deborah Rustichelli, Barbara Roagna, Francesca Fronticelli Bardelli, Franca Fagioli, Ivana Ferrero

“…Morphology, viability, growth kinetics, and immunophenotype were evaluated on healthy donors’ MSCs, treated with triamcinolone acetonide, alcohol-free triamcinolone acetonide, micronized intravitreal triamcinolone and dexamethasone at different concentrations, and in a human retinal pigment epithelial cell line supernatant (ARPE-19). The morphological analysis of MSCs in their standard medium showed a negative correlation with drug concentrations, due to the numerous crystals. …”
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A screening setup to streamline in vitro engineered living material cultures with the host by Krupansh Desai, Shrikrishnan Sankaran, Aránzazu del Campo, Sara Trujillo

“…ELM cytotoxicity on murine fibroblasts and human monocytes was studied by adding ELM supernatants and measuring cell membrane integrity and live/dead staining, respectively, proving ELM cytocompatibility. …”
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Effect of Gamma-Irradiated Recycled Low-Density Polyethylene on the High- and Low-Temperature Properties of Bitumen by Perviz Ahmedzade, Alexander Fainleib, Taylan Günay, Olga Starostenko, Tetyana Kovalinska

“…Superior performing asphalt pavements (Superpave) specifications were used to analyze mechanical test results as well as to determine the performance grades (PG) of the binders. …”
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Berberine Protects against Palmitate-Induced Endothelial Dysfunction: Involvements of Upregulation of AMPK and eNOS and Downregulation of NOX4 by Ming Zhang, Chun-Mei Wang, Jing Li, Zhao-Jie Meng, Sheng-Nan Wei, Ji Li, Richard Bucala, Yu-Lin Li, Li Chen

“…Nitric oxide (NO) level and production of reactive oxygen species (ROS) were determined in supernatants or in the cultured HUVECs. The mRNA level of endothelial nitric oxide synthase (eNOS) was measured by RT-PCR, and the protein levels of eNOS, p-eNOS, Akt, p-Akt, AMPK, p-AMPK, and NADPH oxidase (NOX4) were analyzed. …”
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Exploring the antimicrobial potential of two haloarchaeal strains belonging to the genera Halopiger and Natrialba isolated from the Algerian Sahara by Inès Quadri, Imene Ikram Hassani, Hocine Hacène

“…Producing extracellular compounds associated with halocin, in the cell-free supernatant (CFS). These compounds remained stable at different temperatures (4°C, 60°C, 80°C, and 100°C) and different pH ranges (4-10 and 5-11), with antimicrobial profiles changed in response to UV light. …”
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Biocontrol of gray spot disease on Polygonatum cyrtonema Hua by Bacillus velezensis GX1 by L. Sun, G.L. Deng, H.L. Wu, Q. He, S.H. Hu, J.Y. Li, X.Y. Li, S. Meng, A.N. He, Y.Q. Tian, X. Chen, S.H. Li

“…Notably, a 10 % (v/v) concentration of cell-free supernatant (CFS) of GX1 fully inhibited mycelial growth. …”
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Multiwavelength Variability Analysis of the Blazar PKS 0727-11: An ~ 168 day Quasiperiodic Oscillation in the γ-Ray by Yuncai Shen, Tingfeng Yi, Vinit Dhiman, Lisheng Mao, Liang Dong

“…We demonstrate that QPO arising from the non-ballistic helical jet motion driven by the orbital motion in a supermassive binary black hole is a plausible physical explanation. …”
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IL-21 Modulates Release of Proinflammatory Cytokines in LPS-Stimulated Macrophages through Distinct Signaling Pathways by Su-nan Li, Wei Wang, Shou-peng Fu, Jian-fa Wang, Hong-mei Liu, Shan-shan Xie, Bing-run Liu, Yang Li, Qing-kang Lv, Zhi-qiang Li, Wen-jing Xue, Bing-xu Huang, Wei Chen, Ju-xiong Liu

“…ELISA analysis showed that IL-21 also suppressed LPS-induced production of TNF-α and IL-6 in culture supernatants. Western blot analysis showed that IL-21 clearly inhibited ERK and IκBα phosphorylation and NF-κB translocation in LPS-stimulated macrophages, but it increased STAT3 phosphorylation. …”
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NupR Is Involved in the Control of PlcR: A Pleiotropic Regulator of Extracellular Virulence Factors by Jiaxin Qin, Ziqi Wang, Cheng Qian, Guohui Ji, Yizhuo Zhang, Zhanglei Cao, Bing Yan, Jun Cai

“…In addition, NupR regulates the expression of the PlcR regulon, including hemolysin, phospholipase C, <i>papR</i>, and oligopeptide permease, which could result in the culture supernatant of BMB171 being less virulent to sf9 cells compared to the <i>nupR</i> knockout strain. …”
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Ne pereant fragmenta: l’intelligenza artificiale e la complessità della Corptech governance by Riccardo Michele Colangelo

“…Lungi dalle logiche di una mera opera ricognitiva, si intende sottolineare l’esigenza di superare l’approccio formalistico talora emergente in sede di compliance normativa, valorizzando il ruolo degli assetti digitali ed aprendo la via ad una non procrastinabile attenzione ai profili di Corporate Digital Responsibility.…”
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Comparison of characteristics and immune responses between paired human nasal and bronchial epithelial organoids by Lu Zhu, Wenhao Yang, Jiaxin Luo, Danli Lu, Yanan Hu, Rui Zhang, Yan Li, Li Qiu, Zelian Chen, Lina Chen, Hanmin Liu

“…RSV infection revealed more productive replication in NO, with a downregulated immune pathway identified by RNA sequencing analysis and upregulated levels of pro-inflammatory cytokines in culture supernatants in NO compared to BO. Conclusions NO and BO serve as robust in vitro models, faithfully recapitulating the biological characteristics of upper respiratory epithelial cells. …”
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Effect of Antifungal-Treated Host Macrophages on Candida glabrata by Hong-Bin Li, Na Li, Shu-Ran Wen, Ming-Yue Qiang, Zheng-Hui Yang, Tian-Xiang Dong, Yu-Ye Li, Yi-Qun Kuang

“…Cytokines from infected macrophage supernatants and the levels of superoxide dismutase (SOD) in macrophages were measured at different time points after phagocytosis. …”
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Lactobacillus fermentum and Lactobacillus crispatus Do Not Have Cytotoxic Effects on HN5 Oral Squamous Cell Carcinoma Cell Line by Sepideh Mokhtari, Saede Atarbashi-Moghadam, Elahe Motevaseli, Soudeh Ghafouri-Fard, Ardeshir Hesampour

“…The effects of the supernatant of two lactobacilli, namely, fermentum and crispatus were studied on HN5-cancer cells. …”
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Primordial Germ Cells Quality of Chicken Embryo from Post Cryopreservation Blood Samples by Nurul Azizah, Tatan Kostaman, Yuli Arif Tribudi, Diana Andrianita Kusumaningrum, Suyatno Suyatno, Zultinur Muttaqin, Eko Koswara, Muhammad Gunawan

“…Blood samples that had been filled with 500 µL of phosphate-buffered saline without Ca2+ and Mg2+ (PBS) underwent centrifugation at 1200 rpm for six minutes. The supernatant was discarded and the pellet was mixed with 500 µL 10% FBS-PBS and was then subdivided for cryopreservation and PGCs purification. …”
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Evaluation of the Ability of Miltefosine Associated with Topical GM-CSF in Modulating the Immune Response of Patients with Cutaneous Leishmaniasis by Fábio Peixoto, Maurício T. Nascimento, Rúbia Costa, Juliana Silva, Gaby Renard, Luiz Henrique Guimarães, Gerson Penna, Manoel Barral-Netto, Lucas P. Carvalho, Paulo R. L. Machado, Edgar M. Carvalho

“…Mononuclear cells were stimulated with soluble Leishmania antigen (SLA) on day 0 and day 15 of therapy, and cytokine levels were determined in supernatants by ELISA. The lymphocyte proliferation and oxidative burst were evaluated by flow cytometry, and the degree of infection and Leishmania killing by optical microscopy. …”
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