3D-Printed Anthropomorphic Phantom Framework for Spectral Photon-Counting CT: A Breast Phantom Application by Kyriaki Katsikari, Nadine Francis, Nabil Maalej, Charalampos Pitsalidis, Aamir Younis Raja

“…The breast phantom was created by segmenting an MRI image from the Duke Breast Cancer MRI dataset into two models: one for skin, fibroglandular, and carcinoma tissues, 3D-printed with e-PLA resin, and another for adipose tissue, replicated using paraffin wax. Multi-energy images of the phantom were then evaluated using both narrow and wide energy bins for validation. …”
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Changes in urinary concentrations of contemporary and emerging chemicals in commerce during the COVID-19 pandemic: Insights from the Environmental influences on Child Health Outcom... by Megan E Romano, Jessie P Buckley, Xiuhong Li, Julie B Herbstman, Kurunthachalam Kannan, Sunmi Lee, Susan L Schantz, Leo Trasande, Margaret R Karagas, Frederica Perera, ECHO Cohort Consortium

“…We measured urinary concentrations of analytes across several chemical groups, including polycyclic aromatic hydrocarbons (PAHs), phthalates/alternative plasticizers, synthetic phenols (parabens, bisphenols, triclosan, benzophenones), organophosphate esters (OPEs), insecticides and fungicides. …”
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CLARITY and Light-Sheet microscopy sample preparation in application to human cerebral organoids by T. A. Shnaider, I. E. Pristyazhnyuk

“…One of the key stages of this method is the production of cryo- or paraffin serial sections of samples, which is a very laborious and time-consuming process. …”
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Callus Formation in Fractured Femur of Rats Treated with Injection of Human Umbilical Cord Mesenchymal Stem Cell-Conditioned Medium by Marla Anggita, Widagdo Sri Nugroho, Yuda Heru Fibrianto, Setyo Budhi, Teguh Budipitojo

“…Histologic preparations were done by the paraffin method, by cutting the tissue blocks into 5 μm thickness and then staining with Mallory aniline blue staining. …”
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Expression of Leptin Receptor and Effects of Leptin on Papillary Thyroid Carcinoma Cells by Marilena Celano, Valentina Maggisano, Saverio Massimo Lepore, Marialuisa Sponziello, Valeria Pecce, Antonella Verrienti, Cosimo Durante, Marianna Maranghi, Piernatale Lucia, Stefania Bulotta, Giuseppe Damante, Diego Russo

“…OB-R transcript (in fresh tissues) and proteins (in formalin-fixed and paraffin-embedded specimens) were expressed in all PTC tissues examined, with no significant differences between BRAF-mutated and BRAF-wild-type tumors. …”
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Diagnostic utility of special stains in defining the spectrum of maxillofacial pathologies by Akshay Gupta, Ankita Tandon, Saurabh Juneja, Nikita Gulati, Devi Charan Shetty

“…The cytopathological diagnosis was compared with histopathological diagnosis based on H and E stained sections obtained from paraffin-embedded formalin-fixed biopsy specimen of benign and malignant neoplasms. …”
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Diagnosis of invasive encapsulated follicular variant papillary thyroid carcinoma by protein-based machine learning by Truong Phan-Xuan Nguyen, Minh-Khang Le, Sittiruk Roytrakul, Shanop Shuangshoti, Nakarin Kitkumthorn, Somboon Keelawat

“…We investigated the potential value of a protein biomarker panel defined by machine learning for identifying the invasive encapsulated follicular variant of papillary thyroid carcinoma, initially using formalin-fixed paraffin-embedded samples. Methods We developed a supervised machine-learning model and tested its performance using proteomics data from 46 thyroid tissue samples. …”
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LCZ696 Therapy Reduces Ventricular Tachyarrhythmia Inducibility in a Myocardial Infarction-Induced Heart Failure Rat Model by Po-Cheng Chang, Shien-Fong Lin, Yen Chu, Hung-Ta Wo, Hui-Ling Lee, Yu-Chang Huang, Ming-Shien Wen, Chung-Chuan Chou

“…Although a clinical trial (PARADISE-MI Trial) has been ongoing to examine the effects of LCZ696 in myocardial infarction (MI) patients, the effects of LCZ696 on remodeling of cardiac electrophysiology in animal models remain largely unclear. …”
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Research progress and perspectives on the application of tyramide signal amplification-based multiplex immunohistochemistry/immunofluorescence: a bibliometrics analysis by Xiaotong Yu, Xiaotong Yu, Chen Huang, Chen Huang, Yan Song, Yan Song, Chun Zhang, Chun Zhang, Debo You, Debo You, XuRan Dong, XuRan Dong, DeFu Wu, DeFu Wu, Alan Keith Meeker, Hao Feng, Yuqing Wang, Yuqing Wang

“…Background and aimsMultiplex immunohistochemistry/immunofluorescence (mIHC/IF), which uses the tyramide signal amplification (TSA) technique, enables sequential staining of multiple targets in formalin-fixed paraffin-embedded (FFPE) samples without worrying about cross-reactivity. …”
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Bi-Hormonal Endocrine Cell Presence Within the Islets of Langerhans of the Human Pancreas Throughout Life by Jiwon Hahm, Dawn Kumar, Juan Andres Fernandez Andrade, Edith Arany, David J. Hill

“…Immunofluorescence microscopy was performed for insulin, glucagon, and somatostatin presence on paraffin-embedded sections of pancreata from 20 donors without diabetes aged between 11 days and 79 years of age. …”
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Black Chokeberry (<i>Aronia melanocarpa</i>) Juice Supplementation Affects Age-Related Myocardial Remodeling in Rats by Elena Daskalova, Mina Pencheva, Slavi Delchev, Lyudmila Vladimirova-Kitova, Spas Kitov, Stoyan Markov, David Baruh, Petko Denev

“…After this period, the hearts of the animals were fixed, embedded in paraffin, and immunohistochemical and morphometric analyses were performed. …”
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Loss of p53 Expression in Gastric Epithelial Cells of Helicobacter pylori-Infected Jordanian Patients by Mohammad A. Abu-Lubad, Ghada F. Helaly, Weliam J. Haddadin, Dua’a A. K. Jarajreh, Amin A. Aqel, Munir A. Al-Zeer

“…In this retrospective case-control study, the epithelium of gastric glands in subjects chronically infected with H. pylori was examined for the expression of p53. Paraffin-embedded gastric biopsy samples from the archives for 50 Jordanian patients diagnosed with chronic H. pylori infection and 25 samples free of H. pylori infection and any other gastric abnormalities were selected. …”
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Effects of non-contact electric fields on the kidneys and livers of tumour-bearing rats [version 6; peer review: 1 approved, 2 approved with reservations, 1 not approved] by Luthfi Nurhidayat, Ahmad Ghitha Fadhlurrahman, Firman Alamsyah, Warsito Purwo Taruno, Nisrina Firdausi, Subekti Evi Dwi Nugraheni, Rarastoeti Pratiwi

“…Histopathological cross-sections of the kidneys and livers were made using the paraffin method and Hematoxylin-Eosin staining. Histological scoring used the post-examination masking method with 100 visual fields per group. …”
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PARACETAMOLIO ATPALAIDAVIMO KINETIKA IŠ EKSTEMPORALIAI PAGAMINTŲ REKTALINIŲ SUPOZITORIJŲ by Asta Marija Inkėnienė, Kristina Ramanauskienė, Aurimas Galkontas

“…Jie parodo veiksnių įtaką vaistinės medžiagos atpalaidavimui iš farmacinės formos ir suteikia informacijos apie vaistinės medžiagos atpalaidavimą vaisto veikimo vietoje. …”
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Effect of floral therapy in mothers of premature newborns: A randomized controlled trial by Tatiana Carneiro de Resende, Karla Oliveira Marcacine, Erika de Sá Vieira Abuchaim, Marcia Barbieri, Maria Cristina Gabrielloni, Ana Cristina Freitas de Vilhena Abrão

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Genetic Signatures: CD44 Single-Nucleotide Polymorphisms Affect Cell Surface Expression and Elevate Risk in Head and Neck Squamous Cell Carcinoma by Muhammad Kashif, Shah Jahan, Sadia Minhas, Ali Amar, Romeeza Tahir, Haseeb Nisar, Faheem Shehzad, Abdul Hanan Nagi, Nadeem Afzal

“…DNA from formalin-fixed paraffin-embedded tissue sections was isolated and amplified using targeted primers. …”
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Metagenomic analysis of DNA viruses from posttransplant lymphoproliferative disorders by Vikas R. Dharnidharka, Marianna B. Ruzinova, Chun‐Cheng Chen, Priyanka Parameswaran, Harry O'Gorman, Charles W. Goss, Hongjie Gu, Gregory A. Storch, Kristine Wylie

“…We extracted DNA from stored paraffin‐embedded PTLD tissues at our center, identified viral sequences by metagenomic shotgun sequencing (MSS), and analyzed the data in relation to clinical outcomes. …”
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The Immunohistochemical Prognostic Value of Nuclear and Cytoplasmic Silent Information Regulator 1 Protein Expression in Saudi Patients with Breast Cancer by Bayan Alharbi, Alia Aldahlawi, Mourad Assidi, Fatemah Basingab, Kawther Zaher, Jehan Alrahimi, Sara Mokhtar, Jaudah Al-Maghrabi, Abdelbaset Buhmeida, Kaltoom Al-Sakkaf

“…Methods: A total of 407 BC formalin-fixed paraffin-embedded (FFPE) samples were collected from King Abdulaziz University Hospital, Saudi Arabia. …”
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The effect of replacing red algae on histomorphology and enzymes of digestive tract of Nile tilapia, Oreochromis niloticus by Sanaz Kord, Rahim Abdi, Isaac Zamani, Rhima Peyghan

“…After going through the routine steps of tissue preparation, including dehydration with alcohol, clarification with xylol, blocking with paraffin, cutting with a thickness of 4-6 microns with a microtome, and hematoxylin-eosin staining, finally, the evaluation was done with a light microscope. …”
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A Study Comparing Immunohistochemistry, Fluorescence in situ hybridization, GeneXpert® Breast Cancer STRAT4 Assay for Determining Hormone Receptor, Human Epidermal Growth Factor Re... by Rajaa El Aje, Abdellah Naya, Ayoub Khoaja, Younes Zaid, Mounia Oudghiri, Jodi Weidler, Mehdi Karkouri

“…The Xpert® Breast Cancer STRAT4, an RT-qPCR based system, can be used to classify invasive breast carcinomas based on the assessment of these four biomarkers; Methods: We evaluated ESR1, PGR, ERBB2, and MKi67 mRNA expression by Xpert Breast Cancer STRAT4 and ER, PR, HER2 and Ki67 by IHC (FISH for HER2 IHC 2+) in 200 formalin-fixed paraffin-embedded (FFPE) tissue blocks with invasive breast cancer, collected from the Pathology Department of Casablanca Ibn Rochd University Hospital; Results: Concordance between Xpert ® Breast Cancer STRAT4 and IHC was 93.5% for ER, 83.51% for PR, 95% for HER2 (92% for IHC+FISH), and 81.20% for Ki67 (excluding intermediate IHC Staining 10 ≤ %IHC <20). …”
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