Systematic comparison of quantity and quality of RNA recovered with commercial FFPE tissue extraction kits by Sukoluhle Dube, Sharefa Al-Mannai, Li Liu, Sara Tomei, Satanay Hubrack, Shimaa Sherif, Ayesha Jabeen, Eiman I. Ahmed, Apryl Sanchez, William Mifsud, Davide Bedognetti, Wouter Hendrickx, Christophe M. Raynaud

“…Abstract Background FFPE tissue samples are commonly used in biomedical research and are a valuable source for next-generation sequencing in oncology, however, extracting RNA from these samples can be difficult the quantity and quality achieved can impact the downstream analysis. …”
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An exploratory study on the differential diagnostic indicators between adult systemic EBV-positive T-cell lymphoproliferative disorders and angioimmunoblastic T-cell lymphoma with... by Xiaodan Zheng, Yuanyuan Zheng, Yanlin Zhang, Jianlan Xie, Xiaojing Teng, Kuo Bi, Lan Sun, Xiaowen Huang, Mulan Jin, Xiaoge Zhou

“…Differences in immunophenotype, type of EBV-infected cells, clonality and gene mutations between the two groups of cases were compared by immunohistochemical staining, double-label staining, TCR gene rearrangement and next-generation sequencing analysis. Results 7 cases of adult EBV+ T-LPD: all cases had no more than 1 T follicular helper (THF) marker was expressed, and there were significantly more EBER+/CD3 + cells than EBER+/CD20 + cells; 5 cases had mutation detection results, in which only 1 had the characteristic KMT2D mutation, 2 had TET2 mutations, and no common mutations such as DDX3X were detected.16 cases of AITL with multiple EBV infections: all cases were found to express at least 2 TFH markers, with 87% of them expressing at least 3 TFH markers., and had significantly more EBER+/CD20 + cells than EBER+/CD3 + cells; 4 cases had mutation test results, with mutated high-frequency genes being TET2 (100%, and all of them had 2 or more TET2 mutations) and RHOA G17V (100%), DNMT3A mutation occurred in 2 cases (50%), and IDH2 R172 mutation occurred in 1 case (25%). …”
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EFFECT OF GLOMUS MOSSEAE, AZOTOBACTER CHROOCOCCUM AND VERMICOMPOST TEA ON SOME RHIZOSPHERE CHARACTERS AND MYCORRHIZAL DEPENDENCY OF CUCUMBER PLANTS by Z. H. Al-Rawi, J. S. Alkobaisy

“…Then, the treatment of Microrizae with Azotobacter was supplemented with half the recommended fertilizer application (MA) with 10% vermicompost tea. Next, the Microrizae (M) and Azotobacter (A) treatments were applied individually. …”
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Clinical Outcome and Quality of Life after Modular Reverse Total Shoulder Arthroplasty in Comparison with Joint‐Preserving Locking Plate Osteosynthesis in Aged Patients: A Retrospe... by Carina Jaekel, Lisa Oezel, Franziska Leibnitz, Lena Marie Wilms, Joachim Windolf, Sebastian Viktor Gehrmann, Armin Olaf Scholz

“…While nondisplaced fractures achieve good results by conservative treatment, surgical procedures are discussed controversially. Next to open reduction and internal fixation (ORIF), the primary use of reverse shoulder arthroplasty (RSA) has become increasingly important. …”
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The influence of monosodium glutamate on the development of oxidative-nitrosative stress in the large cerebral hemispheres of rats under the combination of a change in the “light-d... by O. A. Volkova, O. Ye. Akimov, V. O. Kostenko

“…To simulate AD, a normal “light-dark” cycle (12 hours of light, 12 hours of darkness) was formed for 3 weeks, and the next 3 days the “light-dark” phases were shifted back by 6 hours. …”
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Refinement of a meaning-centered counseling program for Chinese patients with advanced cancer: integrating cultural adaptation and implementation science approaches by Florence Lui, Qingyi Zhang, George Chengxi Bao, Bharat Narang, Ruo Yan Chen, Yunshan Niu, Jennifer Leng, William Breitbart

“…Conclusion Recommendations for next steps include increasing the MCP-Ch protocol’s flexibility and adaptability to allow interventionists to flexibly tailor MCP-Ch material to meet patients’ individual needs, simplifying content to improve comprehension and acceptability, providing additional training to Chinese-serving providers to increase adoption and sustainability, and considering interpreter-assisted delivery to increase access. …”
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Differences in expression profiles in malingant melanoma patients according to immunotherapy response by E. N. Lukyanova, M. S. Fedorova, E. A. Pudova, T. V. Nasedkina, E. V. Stepanova, K. M. Nyushko, A. Y. Popov, N. V. Koroban, A. A. Dmitriev, M. V. Kiseleva, A. V. Lipatova, A. S. Zasedatelev, A. V. Kudryavtseva

“…Melanoma is one of the most immunogenic tumors and, as a result, has become a model object for research into and introduction of new approaches to immunotherapy. In this study, we compared two groups of patients with metastatic skin melanoma, with different responses to immunotherapy with blockers of immune control points, to identify new predictive expression biomarkers among microRNAs and mRNAs, and to identify the genes responsible for the occurrence of an objective response to therapy. …”
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Optical properties of repaired additively manufactured resin composites and zirconia and subtractively manufactured ceramics: a comparative study of composite resins by Esma Çağlayan, Rafat Sasany, Burcu Naz Bolat, Didar Hartavi, Oyun-Erdene Batgerel, Sultan Merve Ucar, Dilara Gülhan Umurca

“…Materials and methods Disk-shaped specimens (Ø10 × 2 mm; N = 120) were fabricated using six different material groups: additively manufactured resin composite (AM-RC) materials (Crowntec [C], NextDent [ND]), additively manufactured zirconia (AM-Z) materials (Lithoz [LI], INNI-Cera [IN]), and subtractively manufactured (SM) materials (CEREC Tessera [ALD], Vita Enamic [EN]). …”
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Enhancing newborn screening sensitivity and specificity for missed NICCD using selected amino acids and acylcarnitines by Peiyao Wang, Lingwei Hu, Yuhe Chen, Duo Zhou, Shasha Zhu, Ting Zhang, Ziyan Cen, Qimin He, Benqing Wu, Xinwen Huang

“…Metabolic profiles were analyzed using the non-derivatized MS/MS Kit, and genetic mutations were identified via next-generation sequencing and confirmed by Sanger sequencing. …”
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Image-based anomaly detection in low-light industrial environments with feature enhancement by Dinh-Cuong Hoang, Phan Xuan Tan, Anh-Nhat Nguyen, Son-Anh Bui, Ta Huu Anh Duong, Tuan-Minh Huynh, Duc-Manh Nguyen, Viet-Anh Trinh, Quang-Huy Ha, Nguyen Dinh Bao Long, Duc-Thanh Tran, Xuan-Tung Dinh, Van-Hiep Duong, Tran Thi Thuy Trang

“…Specifically, it achieves a mean image-level anomaly detection accuracy (I-AUROC) of 0.899 and a localization performance (AUPRO) of 0.862, surpassing the next-best method by 16.2% and 16.5%, respectively. …”
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Clinical and molecular characteristics and long-term outcomes of pediatric intracranial meningiomas: a comprehensive analysis from a single neurosurgical center by Leihao Ren, Jiaojiao Deng, Hiroaki Wakimoto, Qing Xie, Ye Gong, Lingyang Hua

“…Method We enrolled 74 patients with intracranial PMs and analyzed their clinicopathological characteristics. Targeted next generation sequencing was used to detect alterations in meningioma relevant genes. …”
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Survival and Its Predictors Among Patients Receiving Transarterial Chemoembolization for Hepatocellular Carcinoma in Ethiopia: A 6-Year Follow-Up Study by Abigia Ashenafi, Soliyana Demelash, Efrata Melaku, Haymanot Abe, Meron Yitna, Fisseha Tekle, Jiksa Dabessa, Biniam Araya, Yared Nigussie, Wondmagegn Demsiss, Ashenafi Zelalem, Song Jung Kim, Nebiyu Dereje

“…Data were extracted from patients' medical records, and vital status was ascertained from the patients' charts or by phone call to the next of kin. We used Kaplan-Meier estimator to determine survival functions and log-rank test to compare the survival functions in different groups. …”
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Mobile-based in-home telerehabilitation compared with in-hospital face-to-face rehabilitation for elderly patients after total hip arthroplasty in China's level 1 trauma center: a... by Yang Zhou, Yiming Lyu, Qiaojie Wang, Yanhong Ma, Lihua Huang, Xin Zhang, Xin Zhang

“…The PT group received a standard in-hospital rehabilitation intervention assisted by a physical therapist for one month and outpatient clinic for the next two months. Patients were evaluated at baseline, 4 weeks, and 12 weeks postoperatively employing functional tests (Timed Up & Go test and Berg balance test) and self-reported questionnaires (Hip disability and Osteoarthritis Outcome Score (HOOS) and Short Form 12 (SF-12)).ResultsThere was no significant difference between the two groups for the demographic and clinical characteristics. 61 participants were analyzed (PT group: n = 31, women: 48.4% of participants; TELE group: n = 30, 33.3% of participants) whose median age was 70 and 69 years, in PT group (IQR: 63–73) and TELE group (IQR: 66–72) respectively. …”
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Targeting HVEM-GPT2 axis: a novel approach to T cell activation and metabolic reprogramming in non-small cell lung cancer therapy by Yuanshan Yao, Chunji Chen, Bin Li, Wen Gao

“…Lung cancer cells co-cultured with Jurkat T cells were xenografted into nude mice to evaluate tumor growth and metastatic potential. Next, RNA-seq, COIP, Dual-luciferase reporter experiment, and CHIP-seq were used to explore the potential underlying mechanism. …”
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A User-Centered Design Approach for a Screening App for People With Cognitive Impairment (digiDEM-SCREEN): Development and Usability Study by Michael Zeiler, Nikolas Dietzel, Fabian Haug, Julian Haug, Klaus Kammerer, Rüdiger Pryss, Peter Heuschmann, Elmar Graessel, Peter L Kolominsky-Rabas, Hans-Ulrich Prokosch

“…This research highlights the significance of a user-centered design approach, allowing content, text, and design to be optimally tailored to the needs of the target audience. …”
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Effect of Acupuncture Exercise Therapy On Isokinetic Muscle Strength and Exercise Ability of Patients after Anterior Cruciate Ligament Reconstruction by Wen YAN, Jiaying MURONG, Shuyi CUI, Wanwen HE, Xun ZHUANG

“…After acupuncture, the next training was carried out. After the training for three items, the patients took a rest for two minutes, and each training was done for three cycles. …”
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Tp53 determines the spatial dynamics of M1/M2 tumor-associated macrophages and M1-driven tumoricidal effects by Yi-Jing Hsiao, Min-Shu Hsieh, Gee-Chen Chang, Yin-Chen Hsu, Chia-Yu Wang, Yan-Ming Chen, Yi-Ling Chen, Pan-Chyr Yang, Sung-Liang Yu

“…We characterized the mutation status and density of M1/M2 TAMs within tumor islets and stroma in 117 lung adenocarcinomas using next-generation sequencing and immunohistochemistry, respectively. …”
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Human-genome single nucleotide polymorphisms affecting transcription factor binding and their role in pathogenesis by E. V. Antontseva, A. O. Degtyareva, E. E. Korbolina, I. S. Damarov, T. I. Merkulova

“…The development of the NGS (next-generation sequencing) technology has contributed not only to the identification of a huge number of SNPs and to the search for their association (genome-wide association studies, GWASs) with certain diseases or phenotypic manifestations, but also to the development of more productive approaches to their functional annotation. …”
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Korean patients with hereditary cancer: a prospective multicentre cohort study protocol exploring psychosocial and health outcomes by Boyoung Park, Sun-young Kong, Dongju Won, Jong Eun Park, Jun-Kyu Kim, Mi-Ae Jang, Jung-Sook Ha, Kyoung-Bo Kim

“…This study evaluates changes in participant quality of life (QoL), unmet needs and mental health over time based on their genetic variant status.Methods and analysis This prospective cohort study will recruit 1435 patients with suspected hereditary cancer who have undergone BRCA1/2 or next-generation sequencing (NGS) testing. …”
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TPDTNet: Two-Phase Distillation Training for Visible-to-Infrared Unsupervised Domain Adaptive Object Detection by Siyu Wang, Xiaogang Yang, Ruitao Lu, Shuang Su, Bin Tang, Tao Zhang, Zhengjie Zhu

“…Enhanced domain data are fed into the teacher network to initialize the weights and produce pseudolabels. Next, to address small remote sensing target detection tasks, we construct a multidimensional progressive feature fusion detection framework, which initially fuses two adjacent low-level feature maps and then progressively incorporates high-level features to enhance the quality of fusing nonadjacent layer features. …”
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