Conversion of placental hemogenic endothelial cells to hematopoietic stem and progenitor cells by Guixian Liang, Shicheng Liu, Chunyu Zhou, Mengyao Liu, Yifan Zhang, Dongyuan Ma, Lu Wang, Jing-Dong J. Han, Feng Liu

“…Mechanistically, we found that VN-ECs can promote the generation of iHSPCs via secretion of CX3CL1 and IL1A. Next, through VN-EC co-culture, we showed that placental HE (pHE) cells, a type of extra-embryonic HE cells, were successfully converted into iHSPCs (pHE-iHSPCs), which have multilineage differentiation capacity, but exhibit limited self-renewal ability. …”
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Probing aspects of extracellular vesicle associated AAV allows increased vector yield and insight into its transduction and immune-evasive properties by Ming Cheng, Demitri de la Cruz, Adam V. Crain, Paula Espinoza, Carrie Ng, Zachary C. Elmore, Aravind Asokan, Casey A. Maguire

“…Despite these promising data, better characterization of EV-AAV is needed. We used density gradient ultracentrifugation to separate EV-AAV from free AAV to determine the yields and functional activity of EV-AAV. …”
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Microstructure, mechanical properties, thermal decomposition and oxidation sequences of crystalline AlB2 thin films by Chun Hu, Stanislav Mráz, Peter J. Pöllmann, T. Wojcik, M. Podsednik, B. Hajas, A. Limbeck, Nikola Koutná, Jochen M. Schneider, Paul H. Mayrhofer

“…Detailed transmission electron microscopy shows that the highly (0001)-oriented AlB2.27 thin film exhibits small platelet-like amorphous B regions next to the large columnar α-structured AlB2 crystals. …”
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Schnittstellen zwischen performance und Analyse von Popmusik. Performative Produktionsprozesse in Pink Floyds Album Wish You Were Here und Jordan Rudessʼ Coverversion von Genesisʼ... by Hubertus Dreyer, Pascal Horn

“…Beide Beispiele gehören zur Progrock-Tradition und weisen ähnliche musikalische Komplexität auf; die Art und Weise dieser Komplexität aber unterscheidet sich und reflektiert unterschiedliche Produktionsstile: Bei Pink Floyd steht ein gleichsam vegetativer, großen psychischen Belastungen entspringender Wachstumsprozess im Vordergrund, der zu einem dichten Netz musikalischer Bezüge innerhalb des Albums führt, bei Jordan Rudess ein rational geplantes, ökonomisches Verfahren, dessen Resultat seine Wirkung nicht zuletzt aus der Virtuosität der Musiker bezieht. // The separation between a ‘work’ and its ‘interpretation’, which has been questioned in recent musical performance studies, is hardly applicable in pop music; performance (especially when it predominantly takes place in the studio) can be understood as an integral aspect of a song or album. …”
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Comparing acute versus AIDS ART initiation on HIV-1 integration sites and clonal expansion by Jun Wang, Nan Xiao, Zhengnong Zhu, Haiyan Qiao, Fang Zhao, Lukun Zhang, Jizhou Gou, Mengji Lu, Yun He, Hongzhou Lu, Qian Li

“…In this study, we used Linear Target Amplification-PCR (LTA-PCR) and Next Generation Sequencing to compare unique integration site (UIS) clonal counts between individuals who initiated ART during acute HIV infection stage (Acute-ART group) and those in the AIDS stage (AIDS-ART group). …”
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Pendekatan Bayes-HDSS dalam Menentukan Status Pantauan Gizi Balita by Riki Hisbullah, Muhammad Siddik Hasibuan

“…The greatest value of the output system provides certainty that children need focus in overcoming the problem of malnutrition. …”
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Design of Conformal Cooling of an Additively Printed Aluminium Die-Casting Mold Component by J. Sviželová, L. Socha, A. Mohamed, M. Pinta, K. Koza, T. Sellner, K. Gryc, M. Dvořák, M. Roh

“…The component will be manufactured and tested in operational conditions in the next research phase.…”
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Structure- and machine learning-guided engineering demonstrate that a non-canonical disulfide in an anti-PD-1 rabbit antibody does not impede antibody developability by Wei-Ching Liang, Hongkang Xi, Dawei Sun, Luigi D’Ascenzo, Jonathan Zarzar, Nicole Stephens, Ryan Cook, Yinyin Li, Zhengmao Ye, Marissa Matsumoto, Jian Payandeh, Matthieu Masureel, Yan Wu

“…Side-by-side developability assessment of both h1340.CC and h1340.SA.LV indicate that they possess similar, favorable properties. Next, and prompted by recent developments in machine learning (ML)-guided protein engineering, we used an unbiased ML- and structure-guided approach to rapidly and efficiently generate a different variant with recovered affinity. …”
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Exome Sequencing Identified a Recessive RDH12 Mutation in a Family with Severe Early-Onset Retinitis Pigmentosa by Bo Gong, Bo Wei, Lulin Huang, Jilong Hao, Xiulan Li, Yin Yang, Yu Zhou, Fang Hao, Zhihua Cui, Dingding Zhang, Le Wang, Houbin Zhang

“…This study is to identify gene mutations responsible for autosomal recessive retinitis pigmentosa (arRP) in a Chinese family using next-generation sequencing technology. A Chinese family with 7 members including two individuals affected with severe early-onset RP was studied. …”
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Evidence-informed language: interpretation and impact on intentions to treat – results of an online survey of medical students and specialists in German-speaking countries by Sven Benson, Reinhard Griebenow, Justine Schmidt, Henrik Herrmann

“…Responses from physicians and medical students were largely comparable.Conclusion This study demonstrates limitations in the currently used linguistic expressions of strength of evidence and clinical practice recommendations and supports the need to prospectively test effects of language on intentions to treat prior to implementation of a certain wording. …”
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In vivo efficacy and safety of systemically administered serinol nucleic acid-modified antisense oligonucleotides in mouse kidney by Toshiki Tsuboi, Keita Hattori, Takuji Ishimoto, Kentaro Imai, Tomohito Doke, Junichiro Hagita, Jumpei Ariyoshi, Kazuhiro Furuhashi, Noritoshi Kato, Yasuhiko Ito, Yukiko Kamiya, Hiroyuki Asanuma, Shoichi Maruyama

“…Nucleic acid medicine encompassing antisense oligonucleotides (ASOs) has garnered interest as a potential avenue for next-generation therapeutics. However, their therapeutic application has been constrained by challenges such as instability, off-target effects, delivery issues, and immunogenic responses. …”
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Empresarios, capitales e industrias en la región Caribe colombiana bajo el despegue del modelo agroexportador a comienzos del siglo XX by SEGIO PAOLO SOLANO

“…Therefore what we show is that there was no continuity line with the business of the nineteenth century and the beginning of the next century, which ended up affecting the scope of maintenance and expansion of industries.…”
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Development of [F]FPy-WL12 as a PD-L1 Specific PET Imaging Peptide by Wojciech G. Lesniak PhD, Ronnie C. Mease PhD, Samit Chatterjee PhD, Dhiraj Kumar PhD, Ala Lisok MA, Bryan Wharram MS, Venkateswara Rao Kalagadda PhD, Leisha A. Emens MD, PhD, Martin G. Pomper MD, PhD, Sridhar Nimmagadda PhD

“…The uptake of the radiotracer reflected the PD-L1 expression assessed by flow cytometry. Next, we performed the in vivo evaluation of [ 18 F]FPy-WL12 in mice bearing cancer xenografts by PET imaging, ex vivo biodistribution, and blocking studies. …”
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The Identification of Molecular Ploidy Status of Abnormal Pronuclear Zygotes Reveals a Significant Number of Euploid Blastocysts Available for Conception by Blair R. McCallie, Mary E. Haywood, Lauren N. Henry, Rachel M. Lee, William B. Schoolcraft, Mandy G. Katz-Jaffe

“…Biopsied trophectoderm cells were then lysed, and the isolated DNA was whole-genome amplified followed by library preparation. Next-generation sequencing was performed for PGT-A, and excess whole-genome amplified DNA was utilized for single nucleotide polymorphism beadchip array analysis. …”
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Targeting unique ligand binding domain structural features downregulates DKK1 in Y537S ESR1 mutant breast cancer cells by K. S. Young, G. R. Hancock, E. C. Fink, A. Zigrossi, B. Flowers, D. A. Cooper, V. T. Nguyen, M. C. Martinez, K. S. Mon, M. Bosland, D. R. Zak, A. P. Runde, M. N. Sharifi, I. Kastrati, D. D. L. Minh, S. Kregel, Sean W. Fanning

“…Importantly, we find that DKK1 is significantly enriched in ER + breast cancer plasma compared to healthy controls. This study shows how new SERMs and SERDs can identify new therapeutic pathways in endocrine-resistant ER + breast cancers.…”
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Measure-and-remeasure as an economically feasible approach to crediting soil organic carbon at scale by Eric Potash, Mark A Bradford, Emily E Oldfield, Kaiyu Guan

“…Moreover, measure-and-remeasure projects provide valuable data for independent validation on commercial farms of the accrual rates estimated by biogeochemical models using field trials. We provide next steps and software for researchers, credit registries, and project developers to move forward with measure-and-remeasure SOC projects.…”
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A novel platform for engineered AAV-based vaccines by Sabrina Babutzka, Miranda Gehrke, Anastasia Papadopoulou, Maria Diedrichs-Möhring, Maria Giannaki, Lena Hennis, Bastian Föhr, Cale Kooyman, Andreas Osterman, Evangelia Yannaki, Gerhild Wildner, Hermann Ammer, Stylianos Michalakis

“…In conclusion, this novel AAV-based next-generation vaccine platform allows for the presentation of large antigenic sequences to elicit strong and specific immune responses. …”
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Analisis Interaksi Aktivitas Pembelajaran Daring Berdasarkan Data Log Aktivitas pada Learning Managemefnt System (LMS) Menggunakan Educational Process Mining by Novriani Dewi Anwar, Nanang Yudi Setiawan, Welly Purnomo

“…We extract event logs from ELING, an LMS platform provided by our faculty. Next, we conducted Process Mining using the Heuristic Miner method from three different Data Warehouse course class lecturers. …”
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Development of a Recombinant Omicron BA.1 Subunit Vaccine Candidate in Pichia pastoris by Sibel Kalyoncu, Dogu Sayili, Ayca Zeybek Kuyucu, Hakan Soyturk, Seyda Gullu, Busra Ersayan, Ibrahim Oguzhan Tarman, Mehmet Ender Avci, Olcay Mert, Umut Haskok, Ege Tekin, Huseyin Akinturk, Ridvan Orkut, Aysegul Demirtas, Idil Tilmensagir, Ceren Ulker, Bilgi Gungor, Mehmet Inan

“…ABSTRACT Low‐cost and safe vaccines are needed to fill the vaccine inequity gap for future pandemics. …”
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Value Propositions for Digital Shared Medication Plans to Boost Patient–Health Care Professional Partnerships: Co-Design Study by Benjamin Bugnon, Francesca Bosisio, Alain Kaufmann, Pascal Bonnabry, Antoine Geissbuhler, Christian von Plessen

“…The 4 value propositions offer strategic guidance, while highlighting the need for further research in different health care settings.…”
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