Significance of Combining Bronchoalveolar Lavage Fluid With Targeted Next‐Generation Sequencing in the Pathogen Detection‐Based Diagnosis of Pulmonary Infections by Jiangbo Liu, Bo Yang, Yu Wu, Guihong Yang, Xiaojiu Zha, Wei Jiang

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A next-generation Fab library platform directly yielding drug-like antibodies with high affinity, diversity, and developability by Fortunato Ferrara, Adeline Fanni, Andre A. R. Teixeira, Esteban Molina, Camila Leal-Lopes, Ashley DeAguero, Sara D’Angelo, M. Frank Erasmus, Laura Spector, Luis Antonio Rodriguez Carnero, Jianquan Li, Thomas J. Pohl, Nikolai Suslov, Klervi Desrumeaux, Conor McMahon, Sagar Kathuria, Andrew R. M. Bradbury

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Enabling next-generation engineered TCR-T therapies based on high-throughput TCR discovery from diagnostic tumor biopsies by Thomas Kuilman, Deborah S. Schrikkema, Jules Gadiot, Raquel Gomez-Eerland, Laura Bies, Julia Walker, Robbert M. Spaapen, Hanna Kok, Demi Houg, Milena Viyacheva, Yvonne B. Claassen, Manuel Saornil, Oscar Krijgsman, Bas Stringer, Huiwen Ding, Anou Geleijnse, Anne C. Meinema, Bianca Weissbrich, Melissa Lancee, Carmen G. Engele, Marianna Sabatino, Pei-Ling Chen, Kenneth Y. Tsai, James J. Mulé, Vernon K. Sondak, Jitske van den Bulk, Noel F. de Miranda, Inge Jedema, John G. Haanen, Jeroen W. J. van Heijst, Ton N. Schumacher, Carsten Linnemann, Gavin M. Bendle

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Embryo Mosaicism Rate in National Referral Hospital of Indonesia Detected Using Next-Generation Sequencing: A Retrospective Study by Achmad Kemal Harzif, Muhammad Ikhsan, Pritta Ameilia Iffanolida, Kresna Mutia, Budi Wiweko, R Muharam, Kanadi Sumapraja, Gita Pratama, Mila Maidarti, Vita Silvana, Amalia Shadrina, Irene Sinta Febriana, Nafi'atul Ummah, Aisyah Retno Puspawardani, Andon Hestiantoro

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Clinical characteristics of pathogens in children with community-acquired pneumonia were analyzed via targeted next-generation sequencing detection by Junhua Zhao, Mingfeng Xu, Zheng Tian, Yu Wang

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Next-generation sequencing and immuno-informatics for designing a multi-epitope vaccine against HSV-1-induced uveitis by He Cao, Zhi Cao, Yue Han, Jing Shan

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Could the Next Platform be the Transformational Platform and Tourism? A Platform Based Expansion Model in the Context of Global Sustainable Tourism by Zeki Akıncı, Gülseren Yurcu, Ayşe Himmetoğlu, Dilara Bahtiyar Sarı

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El nexo migración-desplazamiento-asilo en el orden fronterizo de las cosas. Una propuesta analítica by Gloria Naranjo Giraldo

“…Asumir el reconocimiento de los nexos entre tipos de migración y de los diferentes posicionamientos de las personas, plantea retos de conceptualización en torno a las continuidades entre procesos migratorios, en la perspectiva de superar dualismos teóricos y metodológicos que analizan estas tipologías de manera separada. …”
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Melanoma Mimicking Malignant Peripheral Nerve Sheath Tumor with Spread to the Cerebellopontine Angle: Utility of Next-Generation Sequencing in Diagnosis by Katie Fox Hanson, Paul Birinyi, Ronald Walker, Constantine Raptis, Rebecca Chernock, Jeroen Coppens, Katherine E. Schwetye

“…We present an unusually challenging patient who presented with clinical symptoms involving cranial nerves VII and VIII and a parotid-region mass, which was S100-positive while lacking in melanocytic pigment and markers. …”
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We cannot repeat history again: a call to action to centre indigenous leadership as we prepare for the next pandemic by Kristy Crooks, Fatima Ahmed, Eric N. Liberda, Peter D. Massey, Kylie Taylor, Elizabeth Harwood, Celine Sutherland, Gisele Kataquapit, Katrina Clark, Nicholas D. Spence, Robert J. Moriarity, Hannah Briggs, Leonard J.S. Tsuji, Nadia A. Charania

“…As the global community prepares for the next pandemic, it is critical to prioritise Indigenous leadership and governance within public health responses. …”
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Investigating the Pulmonary Host Response of <i>Acinetobacter baumannii</i> Infection-Associated Pneumonia by Metagenomic Next-Generation Sequencing by Mu-Jung Chou, Chih-Hung Cheng, Hui-Ching Wang, Ming-Ju Tsai, Chau-Chyun Sheu, Wei-An Chang

“…<b>Background:</b> For investigating the host response in <i>Acinetobacter baumannii</i> associated pneumonia, we analyzed the host genetic sequences obtained from metagenomic next-generation sequencing (mNGS). <b>Methods:</b> The samples for mNGS were bronchoalveolar lavage fluid (BALF) collected from the lungs of patients infected with <i>A. baumannii</i> and from patients without bacterial infections. …”
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Methylation Markers for Urine-Based Detection of Bladder Cancer: The Next Generation of Urinary Markers for Diagnosis and Surveillance of Bladder Cancer by Thomas Reinert

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Leveraging hybrid model of ConvNextBase and LightGBM for early ASD detection via eye-gaze analysis by Ranjeet Bidwe, Sashikala Mishra, Simi Bajaj, Ketan Kotecha

“…This proposed work offers • A novel method of ConvNextBase and LightGBM leveraging eye position as a feature for early detection of autistic traits. • A new ConvNextBase architecture proposed with few unfreezed layers and extra dense layers with units of 512 and 128, respectively, and dropout layers with a rate of 0.5 that extract rich, high-level, and more complex features from the images to improve generalization and mitigate overfitting. • A LightGBM model performed classification using 3-fold cross-validation and found the best parameters for bagging_function, feature_fraction, max_depth, Number_of_leaves and learning_rate with values of 0.8, 0.8, −1, 31 and 0.1 respectively, to improve the model's robustness on unseen data.This proposed method is trained and tested on the publicly available Kaggle dataset, and results are benchmarked with other state-of-the-art methods. …”
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In-silico formulation of a next-generation polyvalent vaccine against multiple strains of monkeypox virus and other related poxviruses. by Abu Tayab Moin, Nurul Amin Rani, Rajesh B Patil, Tanjin Barketullah Robin, Md Asad Ullah, Zahidur Rahim, Md Foyzur Rahman, Talha Zubair, Mohabbat Hossain, A K M Moniruzzaman Mollah, Nurul Absar, Mahboob Hossain, Mohammed Abul Manchur, Nazneen Naher Islam

“…Mpox (formerly known as monkeypox) virus and some related poxviruses including smallpox virus pose a significant threat to public health, and effective prevention and treatment strategies are needed. This study utilized a reverse vaccinology approach to retrieve conserved epitopes for monkeypox virus and construct a vaccine that could provide cross-protection against related viruses with similar antigenic properties. …”
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A deep multiple instance learning framework improves microsatellite instability detection from tumor next generation sequencing by John Ziegler, Jaclyn F. Hechtman, Satshil Rana, Ryan N. Ptashkin, Gowtham Jayakumaran, Sumit Middha, Shweta S. Chavan, Chad Vanderbilt, Deborah DeLair, Jacklyn Casanova, Jinru Shia, Nicole DeGroat, Ryma Benayed, Marc Ladanyi, Michael F. Berger, Thomas J. Fuchs, A. Rose Brannon, Ahmet Zehir

“…Previous work has demonstrated that next-generation sequencing data can be used to identify samples with MSI-high phenotype. …”
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Precise Detection of Gene Mutations in Fine-Needle Aspiration Specimens of the Papillary Thyroid Microcarcinoma Using Next-Generation Sequencing by Feng-xia Yu, Min-xia Hu, Han-xue Zhao, Li-juan Niu, Xue-yu Rong, Wei-hua Li, Qiang Zhu, Jian-ming Ying, Ning Lyu

“…To assess the feasibility of next-generation sequencing (NGS) to detect mutations in BRAF, RAS, TERT promoter, and TP53 genes in ultrasound-guided fine-needle aspiration (FNA) biopsy samples of the papillary thyroid microcarcinoma (PTMC). …”
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Targeted Next-Generation Resequencing of Gene Identifies Novel Multiple Variants Pattern in Severe Hereditary Factor V Deficiency by Piotr K. Janicki, Sonia Vaida, Hamid A. B. AL-Mondhiry

“…The present study investigated the genetic defects underlying severe Factor V deficiency in a 26-year-old Columbian (South America) female and her immediate family (both parents and newborn child) by next generation sequencing (NGS) of the entire gene locus. …”
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Cross‐Sectional Imaging Modalities in Correlation to the Thrombolysis in Cerebral Infarction Score: The Next Frontier in Adjunctive Endovascular Stroke Therapy by Johannes Kaesmacher, Kilian M. Treurniet, Manon Kappelhof, Tomas Dobrocky, Johanna Ospel, Adnan Mujanovic, Jens Fiehler, Bernard Yan, Mayank Goyal, Albert J. Yoo, Bruce Campbell, Osama O. Zaidat, Jeffrey L. Saver, Nerses Sanossian, Radoslav Raychev, Yvo B. W. E. M. Roos, Urs Fischer, Charles B. L. M. Majoie, Jan Gralla, David S. Liebeskind

“…Last, we discuss how this definition of the TDT more reliably measures preinterventional reperfusion and establishes homogenous definitions of embolization and infarctions in new territories.…”
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New insights into the phylogeny and infrageneric taxonomy of Saussurea based on hybrid capture phylogenomics (Hyb-Seq) by Liansheng Xu, Zhuqiu Song, Tian Li, Zichao Jin, Buyun Zhang, Siyi Du, Shuyuan Liao, Xingjie Zhong, Yousheng Chen

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Reflections on data network technology by Lintao JIANG

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