A co-registration method to validate in vivo optical coherence tomography in the breast surgical cavity by Rowan W. Sanderson, Renate Zilkens, Peijun Gong, Imogen Boman, Ken Y. Foo, Skandha Shanthakumar, James Stephenson, Wei Ling Ooi, José Cid Fernandez, Synn Lynn Chin, Lee Jackson, Mireille Hardie, Benjamin F. Dessauvagie, Anmol Rijhumal, Saud Hamza, Christobel M. Saunders, Brendan F. Kennedy

“…In this approach, we use ex vivo OCT scans as an intermediary, surgical sutures as fiducial markers, and extend the in vivo field-of-view to 15 × 15 mm2 by acquiring partially overlapping scans. …”
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A stretchable human lung‐on‐chip model of alveolar inflammation for evaluating anti‐inflammatory drug response by Clémentine Richter, Lorenz Latta, Daria Harig, Patrick Carius, Janick D. Stucki, Nina Hobi, Andreas Hugi, Paul Schumacher, Tobias Krebs, Alexander Gamrekeli, Felix Stöckle, Klaus Urbschat, Galia Montalvo, Franziska Lautenschläger, Brigitta Loretz, Alberto Hidalgo, Nicole Schneider‐Daum, Claus‐Michael Lehr

“…For validating the in vitro model, three different protocols have been developed to demonstrate the clinically established anti‐inflammatory effect of glucocorticoids to reduce certain inflammatory markers after different pro‐inflammatory stimuli: (1) an inflammation caused by bacterial LPS (lipopolysaccharides) to simulate an LPS‐induced acute lung injury measured best with cytokine IL‐6 release; (2) an inflammation caused by LPS at ALI (air‐liquid interface) to investigate aerosolized anti‐inflammatory treatment, measured with chemokine IL‐8 release; and (3) an inflammation with a combination of human inflammatory cytokines TNFα and IFNγ to simulate a critical cytokine storm leading to epithelial barrier disruption, where the eventual weakening or protection of the epithelial barrier can be measured. …”
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Clarification of factors associated with post-artesunate delayed hemolysis (PADH): Analysis of 327 patients with severe imported Plasmodium falciparum malaria in France by Pierre-Louis Conan, Marc Thellier, Eric Kendjo, Sandrine Houzé, Rémonie Seng, Stéphane Jauréguiberry

“…Background: Post-Artesunate delayed hemolysis (PADH) occurs in approximately 15 % of treated patients 2–3 weeks after artesunate administration. Identifying risk markers for PADH would help predict which patients are at higher risk. …”
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Epidemiology of chronic and acute pancreatitis in India (EPICAP-India): protocol for a multicentre study by Sanjeev Kumar, Saransh Jain, Vineet Ahuja, Rakesh Kumar, Anand Krishnan, Pankaj Bhardwaj, Shalimar, Nitya Wadhwa, Ashish Agarwal, Deepak Gunjan, Sudipta Dhar Chowdhury, Anoop Saraya, Saurabh Kedia, Govind K Makharia, Pramod Kumar Garg, Ritvik Amarchand, Deepti Dabar, Rajib Sarkar, Gopal Krishna Dhali, Anmol Gupta, Shivendra Singh, Divya Pillai, Vineeta Baloni, Subhra Samujjwal Basu, Bikash Choudhury, Soumi Das, Pradeep Deshmukh, Krishnadas Devadas, Nitika Monga, Sumit Rungta, Brij Sharma, Chintha Sujatha

“…For each case, four controls will be selected and data on risk factors for CP (tobacco, alcohol, family history, metabolic causes) and blood for genetic markers will be collected. Information on the cost of treatment and quality of life will be collected from patients with CP. …”
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Development of Collagen-Based 3D Matrix for Gastrointestinal Tract-Derived Organoid Culture by Joo Hyun Jee, Dong Hyeon Lee, Jisu Ko, Soojung Hahn, Sang Yun Jeong, Han Kyung Kim, Enoch Park, Seon Young Choi, Sukin Jeong, Joong Woon Lee, Han-Jun Cho, Min-Soo Kwon, Jongman Yoo

“…Then, characteristics of mouse colon organoids were analyzed by morphology and quantitative messenger RNA (mRNA) expression, revealing that the mouse colon organoids grown in the collagen-based matrix and in Matrigel had quite similar morphology, specific markers, and proliferative rates. Mouse small intestine–derived organoids, stomach-derived organoids, and human colon–derived organoids were also cultured, all of which were successfully grown in the collagen-based matrix and had similar properties compared to those cultured in Matrigel. …”
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Characterization of terpenoids and norisoprenoids from base and retail Qingke Baijiu by GC × GC-TOFMS and multivariate statistical analysis by Xinlei Wang, Lin Zhu, Xuebo Song, Si Jing, Fuping Zheng, Mingquan Huang, Shengbao Feng, Luzhong La

“…The terpenoids and norisoprenoids, such as (E)-β-ionone, isoborneol and β-cyclocitral, could be potential markers indicating the ageing process of Qingke Baijiu.…”
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Antidepressant Effects of Probucol on Early-Symptomatic YAC128 Transgenic Mice for Huntington’s Disease by Cristine de Paula Nascimento-Castro, Ana Claudia Wink, Victor Silva da Fônseca, Claudia Daniele Bianco, Elisa C. Winkelmann-Duarte, Marcelo Farina, Ana Lúcia S. Rodrigues, Joana Gil-Mohapel, Andreza Fabro de Bem, Patricia S. Brocardo

“…The motor performance and affective symptoms were monitored using well-validated behavioral tests in YAC128 mice and age-matched wild-type littermates at 2, 4, and 6 months of age, after 1, 3, or 5 months of treatment with probucol (30 mg/kg/day via water supplementation, starting on postnatal day 30). Endogenous markers were used to assess the effect of probucol on cell proliferation (Ki-67 and proliferation cell nuclear antigen (PCNA)) and neuronal differentiation (doublecortin (DCX)) in the hippocampal dentate gyrus (DG). …”
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Molecular evidence for the efficacy of PBM therapy in the treatment of oral lichen planus by Maria Zaharieva Mutafchieva, Milena Nenkova Draganova, Plamen Ivanov Zagorchev, Reem Hanna, Georgi Tomchev Tomov

“…Tissue sections from 10 healthy volunteers were used as controls for the levels of these markers in normal oral mucosa. Results: PBM significantly reduced patients’ pain intesity and improved the clinical signs of the lesions. …”
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Resveratrol enhances post-injury muscle regeneration by regulating antioxidant and mitochondrial biogenesis by Xiaoli Qin, Wenjing Niu, Kai Zhao, Yawen Luo, Wenfang Wang, Yang He, Fuyu Yang, Binghai Cao, Min Du, Huawei Su

“…Notably, gene expression analyses demonstrated that RES-treated mice exhibited elevated levels of myogenic markers, such as paired box 7 (Pax7), myogenic factor 5 (Myf5), myoblast determination protein (MyoD), and Myogenin (MyoG). …”
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Quaternary ammoniums activate human dendritic cells and induce a specific T-cell response in vitro by Marine Peyneau, Mathilde Zeller, Virginie Paulet, Benoît Noël, Marie-Hélène Damiens, Natacha Szely, Andreas Natsch, Marc Pallardy, Sylvie Chollet-Martin, Luc de Chaisemartin, Saadia Kerdine-Römer

“…Results: Amongst the seven molecules tested, four could modulate activation markers on DCs, and thus can be classified as chemical sensitizers (polyquaterniums-7 and -10, ethylhexadecyldimethylammonium and benzethonium). …”
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Sun-Drying and Melatonin Treatment Effects on Apricot Color, Phytochemical, and Antioxidant Properties by Rukiye Zengin, Yılmaz Uğur, Yasemin Levent, Selim Erdoğan, Harlene Hatterman-Valenti, Ozkan Kaya

“…Specifically, the treatment resulted in improved color retention (increased L*, a*, and b* values), reduced oxidative stress markers (MDA and H₂O₂), and optimized sugar composition (glucose: 18.99 g/100 g, fructose: 12.58 g/100 g, sucrose: 15.52 g/100 g). …”
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Phenotypic and genotypic characterization of Mycobacterium tuberculosis pyrazinamide resistance—India, 2018–2020 by Sembulingam Tamilzhalagan, Evanslin Santus Justin, Ashok Selvaraj, Karthick Venkateswaran, Arun Kumar Sivakumar, Suganthi Chittibabu, Heather P. McLaughlin, Patrick K. Moonan, Jonathan P. Smith, Sakthi Suba, Mukesh Kumar Sathya Narayanan, Christine S. Ho, Nishant Kumar, Srikanth P. Tripathy, Srikanth P. Tripathy, Siva K. Shanmugam, Patricia J. Hall-Eidson, Uma Devi Ranganathan

“…PZA resistance was predominately observed in Lineage 2 and eight lineage-specific resistance markers were identified. Mutations distributed across pncA correlate to 94% of PZA resistance and were the predominant drivers of phenotypic resistance; evidence generated herein substantiates sequencing the entire gene and promoter for comprehensive genotypic-based prediction of PZA resistance. …”
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The Effects of Krill Oil on mTOR Signaling and Resistance Exercise: A Pilot Study by John Georges, Matthew H. Sharp, Ryan P. Lowery, Jacob M. Wilson, Martin Purpura, Troy A. Hornberger, Flint Harding, James H. Johnson, David M. Peele, Ralf Jäger

“…Krill oil significantly stimulated mTOR signaling in comparison to S-PC and control. No differences for markers on the CMP, CBC, or UA were observed. Krill oil significantly increased lean body mass from baseline (p=0.021, 1.4 kg, +2.1%); however, there were no statistically significant differences between groups for any measures taken. …”
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Protective role of vitamin D receptor against mitochondrial calcium overload from PM2.5-Induced injury in renal tubular cells by Mengqiu Lu, Zishun Zhan, Dan Li, Hengbing Chen, Aimei Li, Jing Hu, Zhijun Huang, Bin Yi

“…Mitochondrial function, calcium concentration, and oxidative stress markers were assessed. VDR activation, achieved through genetic overexpression and paricalcitol, was induced to examine its effect on mitochondrial calcium uniporter (MCU) expression and mitochondrial calcium regulation. …”
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Comparative plastome analyses and evolutionary relationships of 25 East Asian species within the medicinal plant genus Scrophularia (Scrophulariaceae) by Xia Wang, Lei Guo, Lulu Ding, Leopoldo Medina, Ruihong Wang, Pan Li

“…Through genome alignment of these 25 species, 38-53 repeated sequences and 7 shared SSRs were identified, along with regions with high nucleotide polymorphism (Pi), which could potentially serve as molecular markers for species identification. The genome structure, gene content, and arrangement showed conservation, while variations were observed in the IR boundary regions and IGS. …”
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The Characteristics of Sex Differentiation Based on Morphological Traits During the Early Development Stage of the Swimming Crab <i>Portunus trituberculatus</i> and Sex Prediction... by Yanqing Peng, Junkai Lu, Ronghua Li, Chendong Xu, Shangbiao Zheng, Zhiming Ren, Chuan He, Changkao Mu, Weiwei Song, Chunlin Wang

“…To investigate the morphological traits of different sexes during the early development stage of the swimming crab <i>Portunus trituberculatus</i>, the genetic sex of 405 crablets from stages C I–V was determined using sex-linked molecular markers. The external appearance of each crab was observed, and the morphological traits, including carapace length (CL), full carapace width (FCW), abdomen width (AW), and abdomen length (AL), were measured to compare the differences between the sexes and to develop non-intrusive methods for sex identification. …”
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Clinicopathological relationship between PM2.5 exposure and acute exacerbations of chronic obstructive pulmonary disease by Anfeng Cui, Yingying Shao, Yang Wang, Haodong Wang, Jiayi Song, Caiji Liu, Ningning Shen, Zhiqing Yang, Wangliang Zhang, Tiane Luo, Chen Wang

“…Among the patients, 76.5 % were smokers and 68.4 % were over 60 years old. Inflammatory markers (leukocyte and neutrophil counts, eosinophil percentages, C-reactive protein), D-dimer levels, PaCO2, and pulmonary fibrosis indicators showed positive correlations with PM2.5 levels, whereas Pulmonary Function measures (FEV1/ FVC, FEV1 %) and PaO2 showed inverse correlations. …”
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Pluripotency of Dental Pulp Cells and Periodontal Ligament Cells Was Enhanced through Cell-Cell Communication via STAT3/Oct-4/Sox2 Signaling by Zhengjun Peng, Lu Liu, Wenyu Zhang, Xi Wei

“…Alternation in culture environment due to cell-cell communications can rejuvenate the biological activity of aged/differentiated cells and stimulate the expression of pluripotency markers. Dental pulp cells (DPCs) and periodontal ligament cells (PDLCs) are promising candidates in dental tissue regeneration. …”
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Health implications of racial differences in serum growth differentiation factor levels among men with obesity by Siresha Bathina, Virginia Fuenmayor Lopez, Mia Prado, Elliot Ballato, Georgia Colleluori, Maryam Tetlay, Dennis T Villareal, Sanjay Mediwala, Rui Chen, Clifford Qualls, Reina Armamento‐Villareal

“…The objective of this study was to investigate the racial/ethnic differences on the relationship between GDF15 and markers of glucometabolic status, hormonal profile, body composition and bone mineral density (BMD) in obese men. …”
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Invasive mosquito species Aedes albopictus and Aedes aegypti on the Black Sea coast of the Caucasus: genetics (COI, ITS2), Wolbachia and Dirofilaria infections by E. V. Shaikevich, I. V. Patraman, A. S. Bogacheva, V. М. Rakova, O. Р. Zelya, L. A. Ganushkina

“…Another objective was obtaining the genetic characteristic of laboratory strain Ae. aegypti IMPITM. We investigated two markers of nuclear and mitochondrial DNA from Ae. albopictus and Ae. aegypti and compared them to DNA from Ae. cretinus and Ae. koreiсus sympatrically inhabiting the territory, as well as to one of Ae. aegypti from a laboratory line. …”
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