Showing 81 - 100 results of 1,911 for search 'LMP3~', query time: 2.34s Refine Results
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    重组腺病毒载体Ad-LMP-1的构建及其转染 MSC后成骨活性

    Published 2010-01-01
    “…【目的】 快速构建大鼠LIM矿化蛋白-1基因重组腺病毒载体,转染MSC,探讨LMP-1基因对MSC成骨分化和成骨活性的影响65377; 【方法】 从大鼠成骨细胞内提取总RNA,并设计LMP-1特异性引物进行RT-PCR,获取LMP-1基因后利用Invitrogen公司的TOPO定向克隆技术创建入门克隆pENTR/D-LMP-165377;转化TOP10细菌后,挑取阳性克隆摇菌提取质粒,入门克隆再与表达载体pAD/CMV/V5-DEST进行同源重组反应得到病毒载体pAD/CMV/V5-LMP-1,转化细菌后挑选阳性克隆摇菌提取重组病毒质粒,用PacI内切酶线性化后用转染293A细胞后得到重组腺病毒载体65377;以腺病毒为载体,将大鼠LMP-1基因体外转染于3代大鼠MSC细胞内,检测LMP-1基因在MSC细胞的表达,分别观察转染后实验组与对照组I型胶原65380;碱性磷酸酶65380;骨钙素表达变化以及骨钙结节的形成情况,评价LMP-1基因的成骨能力65377;【结果】 成功获取大鼠LMP-1基因,入门质粒与病毒表达质粒经过酶切鉴定以及测序验证65377;LMP-1基因能在MSC中高效表达,转然后MSC的I型胶原,碱性磷酸酶65380;骨钙素的表达明显增强,骨钙结节形成增多65377; 【结论】 利用Gateway 技术构建LMP-1重组腺病毒载体相对简单,可快捷获得的pAd-LMP-165377;pAd-LMP-1转染MSC后,LMP-1基因能促进MSC向成骨细胞分化,增强其成骨作用65377;…”
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  8. 88

    Oscillatory autophagy induction is enabled by an updated AMPK-ULK1 regulatory wiring. by Orsolya Kapuy, Marianna Holczer, Luca Csabai, Tamás Korcsmáros

    Published 2024-01-01
    “…In this study, we demonstrate that oscillatory behaviour in the regulation of the non-selective, stress-induced macroautophagy arises from a series of interlinked negative and positive feedback loops within the mTORC1-AMPK-ULK1 regulatory triangle. While many of these interactions have been known for decades, recent discoveries have revealed how mTORC1, AMPK, and ULK1 are truly interconnected. …”
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  9. 89

    On the Performance of Rate-Compatible LDPC Codes under All-Integer Quantization Decoding by Patinya Muangkammuen, Wongpram Vongjampa, Puripong Suthisopapan

    Published 2022-01-01
    “…Moreover, this simple quantization scheme can also be applied to the hardware and cost-efficient rate-compatible LDPC codes.…”
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    Synthesis and In Vitro AMPK Activation of Cycloalkyl/Alkarylbiguanides with Robust In Vivo Antihyperglycemic Action by Erika Gutierrez-Lara, Carlos Martínez-Conde, Edgar Rosales-Ortega, Juan José Ramírez-Espinosa, Julio C. Rivera-Leyva, David Centurión, Karla Carvajal, Daniel Ortega-Cuellar, Samuel Estrada-Soto, Gabriel Navarrete-Vázquez

    Published 2017-01-01
    “…Compounds were in vitro evaluated as activators of AMP-Activated Protein Kinase (AMPK). The results indicated that compounds 4, 5, and 6 showed similar or even better effect compared to metformin. …”
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    Ekstraksi Benda Asing Lampu Led di Bronkus dengan Bronkoskop Kaku by Fachzi Fitri, Tuti Nelvia

    Published 2014-09-01
    “…</p><p>Keywords: foreign body aspiration, LED lamp, rigid bronchoscope</p>…”
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    Grouping Formation and Obstacle Avoidance Control of UAV Swarm Based on Synchronous DMPC by Yunfeng He, Xianjun Shi, Jianhua Lu, Chaolun Zhao, Guorong Zhao

    Published 2024-01-01
    “…By combining the velocity obstacle method with synchronous DMPC and providing corresponding terminal components, a leader layer control law is designed. …”
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    Reduction of the Impact of Fertilization and Irrigation On Processes in the Nitrogen Cycle in Vegetable Fields with BMPs by Eve-Marie Cockx, Eric H. Simonne

    Published 2003-12-01
    “…HS948/HS201: Reduction of the Impact of Fertilization and Irrigation On Processes in the Nitrogen Cycle in Vegetable Fields with BMPs (ufl.edu) …”
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