Exercise-induced anxiety impairs local and systemic inflammatory response and glucose metabolism in C57BL/6J mice by I. Gálvez, M.C. Navarro, S. Torres-Piles, L. Martín-Cordero, M.D. Hinchado, E. Ortega

“…Phagocytic/microbicide activity, the expression of M1/M2 phenotype markers (CD11c, iNOS, CD206, ARG-1) and cytokines of the inflammatory response (MCP-1, IL-8, IL-6, TNF-α, TGF-β, IL-10) of peritoneal macrophages were determined via flow cytometry. Adipose tissue macrophage infiltration was studied through fluorescence immunohistochemistry. …”
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DRG2 levels in prostate cancer cell lines predict response to PARP inhibitor during docetaxel treatment by Jeong Min Lee, Won Hyeok Lee, Seung Hyeon Cho, Jeong Woo Park, Hyuk Nam Kwon, Ji Hye Kim, Sang Hun Lee, Ji Hyung Yoon, Sungchan Park, Seong Cheol Kim

“…Cells were transfected with DRG2 siRNA, and pcDNA6/V5-DRG2 was used to overexpress DRG2. Flow cytometry was applied for cell cycle assay and apoptosis analysis using the Annexing V cell death assay. …”
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Навчання моделей згорткових нейронних мереж виявленню об’єктів, сцен і контекстів на зображеннях... by Роман Орлов, Сергій Таборанський

“…У дослідженні використовуються Python, TensorFlow та Keras для створення та навчання CNN на наборі даних CIFAR-10. …”
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Identification of the Transcriptional Networks and the Involvement in Angiotensin II-Induced Injury after CRISPR/Cas9-Mediated Knockdown of Cyr61 in HEK293T Cells by Junjie Wang, Dongdong Fu, Soulixay Senouthai, Yan Jiang, Rentong Hu, Yanwu You

“…CCK8, western blotting, and flow cytometry analysis were carried out to dissect cellular function. …”
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The “guild” concept in riparian ecology: Use, limitations and prospects for improving the reproducibility and transferability of results by Philippe Janssen

“…This was formalized by Merritt et al. 2010 with the concept of “riparian vegetation-flow response guilds”, with the aims to develop general frameworks and transfer patterns from one system to another. …”
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Preventing water pollution using importance-performance analysis and terrain analysis by L. Sulistyowati, N. Andareswari, F. Afrianto, A. Rais, M.F. Hafa, D. Darwiyati, A.L. Ginting

“…The terrain analysis results demonstrated that business and industrial activities were distributed in locations with high flow accumulation values, indicating that the water pollution in Batu City was triggered by the presence of business and industrial activities in the watershed accumulation areas. …”
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Development of a Robust UPLC Method for Simultaneous Determination of a Novel Combination of Sofosbuvir and Daclatasvir in Human Plasma: Clinical Application to Therapeutic Drug Mo... by Naser F. Al-Tannak, Ahmed Hemdan, Maya S. Eissa

“…The adopted chromatographic separation was achieved on Waters® Acquity UPLC BEH C18 column (2.1 × 50 mm, 1.7 µm) as a stationary phase using isocratic elution using a mobile phase system of ammonium formate (pH 3.5; 5 mM) and acetonitrile (60:40 v/v) pumped at a flow rate of 0.2 mL.min−1. The UV detection was carried out at 261 nm for SF and 318 nm for DC and IS. …”
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CD276 regulates the immune escape of esophageal squamous cell carcinoma through CXCL1–CXCR2 induced NETs by Yu Liang, Shuang Chen, Zhi Chen, Fang Peng, Qianwen Liu, Gan Xiong, Caihua Zhang, Maosheng Cheng, Rongsong Ling, Demeng Chen, Qimin Zhou

“…Furthermore, we used the 4NQO-induced mouse model to evaluate the effects of anti-CXCL1 antibodies, anti-Ly6G antibodies, anti-NK1.1 antibodies, and GSK484 inhibitors on tumor growth. Moreover, IHC, flow cytometry, and immunofluorescence techniques were employed to measure immune cell proportions in ESCC. …”
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Can electronic monitoring with a digital smart spacer support personalised medication adherence and inhaler technique education in patients with asthma?: Protocol of the randomised... by Job F. M. van Boven, Boudewijn H.J. Dierick, Maria Achterbosch, Sandra Been-Buck, Titia Klemmeier, Susanne J. van de Hei, Paul Hagedoorn, Huib A.M. Kerstjens, Janwillem W.H. Kocks

“…The rechargeable CE-marked smart spacer (Aerochamber Plus with Flow Vu) includes a sensor that monitors adherence and inhalation technique to prescribed dosing regimen of both maintenance and reliever inhalers. …”
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Formulation and Development of a Water-in-Oil Emulsion-Based Luliconazole Cream: In Vitro Characterization and Analytical Method Validation by RP-HPLC by Vijay Kumar Panthi, Utsav Nepal

“…In this study, an analytical C18 (4.6 mm × 25 cm), 5 μm column was used for chromatographic separation with a mixture of acetonitrile and water in the proportion of 50 : 50 v/v as the mobile phase at a flow rate of 1.0 mL/min. The calibration curve was obtained linear at 296 nm in the concentration range of 0.08–0.12 mg/mL. …”
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Molecular mechanism underlying cardioprotective effect of dehydroepiandrosterone on endoplasmic reticulum stress induced apoptosis in human vascular smooth muscle cells and human u... by Ye Zhu, Junxiu Wei, Xin Yang, Wei Zhu

“…DHEA was utilized to inhibit the apoptotic effects of DTT, while estrogen receptor (ER) antagonists ICI 182,780 and G15, the androgen receptor (AR) antagonist flutamide and the aromatase inhibitor letrozole were used to identify the receptors activated during DHEA treatment in HVSMCs and HUVECs. Flow cytometry assessed the apoptotic rate, and Western blotting analysis evaluated the expression levels of ERS-related proteins GRP78 and PERK, and the apoptotic protein marker CHOP. …”
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Evaluating multiannual sedimentary nutrient retention in agricultural two-stage channels by Kaisa Västilä, Tom Jilbert

“…The pilot TSC floodplain significantly retained SS and nutrients while the low-flow channel did not suffer from siltation, supporting the hypothesized enhanced self-cleansing capacity of TSCs compared to trapezoidal cross-sections. …”
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circHUWE1 Exerts an Oncogenic Role in Inducing DDP-Resistant NSCLC Progression Depending on the Regulation of miR-34a-5p/TNFAIP8 by Xueliang Yang, Quan Sun, Yongming Song, Wenli Li

“…Cell apoptosis and cell cycle distribution were determined by flow cytometry. Migrated and invaded cell numbers were examined by transwell assay. …”
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Evaluating factors that impact scoring an open response situational judgment test: a mixed methods approach by Muhammad Zafar Iqbal, Rodica Ivan, Colleen Robb, Jillian Derby

“…Mixed results were found with respect to construct irrelevant factors, such as the flow, cohesion, and kinds of phrases used in the response.ConclusionThis mixed methods study contributes to the construct validity of SJTs by investigating construct relevant and irrelevant factors that may impact assessors’ evaluation of open responses. …”
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A model for predicting acute heart failure in patients with acute myocardial infarction by taking into account energy and adipokine metabolism indicators by M.Yu. Koteliukh

“…The model consisted of fixed effects, namely, two one-factor indicators (HOMA-IR and systolic blood pressure (BP)), one two-factor indicator (systolic and diastolic BP) and one three-factor indicator (adropin, irisin and CTRP3) and random effects such as four one-factor indicators (FABP4, TIMI Grade Flow, platelets, total cholesterol). The accuracy of predicting the absence of Killip class I AHF was 100%, Killip class II AHF – 0%, Killip class III AHF – 11%, Killip class IV AHF – 82%. …”
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Synthesized 18-Lead Electrocardiogram in Diagnosing Posterior Stemi-Equivalent Acute Coronary Syndrome in Patients with NSTEMI by Tomoki Horie, Rikuta Hamaya, Tomoyo Sugiyama, Hidenori Hirano, Masahiro Hoshino, Yoshihisa Kanaji, Tetsumin Lee, Taishi Yonetsu, Tetsuo Sasano, Tsunekazu Kakuta

“…LCx-culprit ACS was defined as thrombolysis in myocardial infarction (TIMI) 0–1 flow. The association between sV7-9 STE and myocardial damage was also assessed. …”
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Differentiation Potential of Early- and Late-Passage Adipose-Derived Mesenchymal Stem Cells Cultured under Hypoxia and Normoxia by Ashley G. Zhao, Kiran Shah, Julien Freitag, Brett Cromer, Huseyin Sumer

“…Furthermore, expression of these genes reduced with passage number, with the exception of PPARG, an adipose differentiation marker, possibly due to the adipose origin of the MSCs. We confirmed by flow cytometry the presence of cell surface markers CD105, CD73, and CD90 and lack of expression of CD45, CD34, CD14, and CD19 across all conditions. …”
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Thermal enhancement using variable characteristics and tripartite diffusion features of solar aircraft wings in context of Reiner-Philippoff hybrid nanofluid passing through a para... by Esraa N. Thabet, A.M. Abd-Alla, S.M.M. El-Kabeir

“…For this investigation, the non-Newtonian Reiner-Philippoff model; a renowned and cutting-edge type of thermally efficient fluid as well as the stability triple diffusive boundary layer natural convective flow contained in a Darcy-Forchheimer porous medium have been taken into consideration. …”
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ITGA4 as a potential prognostic and immunotherapeutic biomarker in human cancer and its clinical significance in gastric cancer: an integrated analysis and validation by Jiaxing Zhang, Jiaxing Zhang, Gang Wang, Jie Liu, Futian Tang, Song Wang, Yumin Li, Yumin Li

“…However, its role across different cancer types is not well understood.MethodsUtilizing multi-omics data, we comprehensively evaluated ITGA4’s expression, clinical relevance, diagnostic and prognostic value, functions, mutations, and methylation status, along with its impact on immunity, mismatch repair (MMR), heterogeneity, stemness, immunotherapy responsiveness, and drug resistance in pan-cancer, with partial validation in gastric cancer (GC) using transcriptomic analysis, single-cell data, western blot (WB), wound-healing assay, flow cytometry and immunohistochemistry (IHC). We further investigated its correlation with clinicopathology and serological markers on tissues from 80 GC patients.ResultsITGA4 expression was generally low in normal tissues but varied significantly across tumor types, with higher levels in advanced stages and grades. …”
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How effective is the addition of specific exercise therapy for patients after anterior cruciate ligament surgery? A systematic review and meta-analysis by Hao Zhou, Jia Qian, Yu-Mei Xing, Long Cui, Yi-Feng Bu

“…Study quality was assessed using the Cochrane Risk-of-Bias tool.Study selectionA total of 11 randomized controlled trials (whole-body vibration training = 4, core-stability training = 2, strength training = 3, blood flow restriction training = 1, and aquatic training = 1) involving 552 anterior cruciate ligament surgery patients were included.Data extractionTwo researchers individually screened the key information for each eligible study and evaluated the quality of the studies. …”
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