ECM-mimicking hydrogel models of human adipose tissue identify deregulated lipid metabolism in the prostate cancer-adipocyte crosstalk under antiandrogen therapy by Agathe Bessot, Joan Röhl, Maria Emmerich, Anton Klotz, Akhilandeshwari Ravichandran, Christoph Meinert, David Waugh, Jacqui McGovern, Jenni Gunter, Nathalie Bock

“…Using extracellular matrix (ECM)-mimicking hydrogel coculture models of human adipocytes and prostate cancer cells, we show that adipocytes from subcutaneous or bone marrow fat have dissimilar responses under the antiandrogen Enzalutamide. …”
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Targeting SLITRK4 Restrains Proliferation and Liver Metastasis in Colorectal Cancer via Regulating PI3K/AKT/NFκB Pathway and Tumor‐Associated Macrophage by Xiaojiao Sun, Junling Zhang, Bingqi Dong, Qingqing Xiong, Xin Wang, Yanlun Gu, Zhiqi Wang, Huiyu Liu, Jixin Zhang, Xu He, Hongjin Liu, Yi Zhong, Chuxiao Yi, Xiaowei Chi, Zhenming Liu, Xiaocong Pang, Yimin Cui

“…Furthermore, the results from coculture models and single‐cell RNA sequencing analyses suggested SLITRK4 promoted tumor‐associated macrophages (TAMs) infiltration and polarization. …”
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Mesangial Cells Exhibit Features of Antigen-Presenting Cells and Activate CD4+ T Cell Responses by Hongyu Yu, Shaoyuan Cui, Yan Mei, Qinggang Li, Lingling Wu, Shuwei Duan, Guangyan Cai, Hanyu Zhu, Bo Fu, Li Zhang, Zhe Feng, Xiangmei Chen

“…Polarization of naïve CD4+ T cells (Th0 cells) towards the Th1 phenotype was induced by coculture with activated mesangial cells, and the resulting Th1 cells showed increased mRNA and protein expression of inflammation-associated genes. …”
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Antioxidant Properties of Tonsil-Derived Mesenchymal Stem Cells on Human Vocal Fold Fibroblast Exposed to Oxidative Stress by Sung-Chan Shin, Hyung-Sik Kim, Yoojin Seo, Cho Hee Kim, Ji Min Kim, Hyun-Keun Kwon, Jin-Choon Lee, Eui-Suk Sung, Byung-Joo Lee

“…TMSCs also downregulated tBHP-mediated production of proinflammatory cytokines in hVFFs. In addition, coculture with TMSC could restore the endogenous matrix metalloproteinase (MMP) activity of hVFFs upon tBHP treatment and, in turn, reduce the oxidative stress-induced ECM accumulation in hVFFs. …”
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Stem Cells from Human Exfoliated Deciduous Teeth Ameliorate Diabetic Nephropathy In Vivo and In Vitro by Inhibiting Advanced Glycation End Product-Activated Epithelial-Mesenchymal... by Nanquan Rao, Xiaotong Wang, Jing Xie, Jingzhi Li, Yue Zhai, Xiaoxia Li, Tengjiaozi Fang, Yuanyuan Wang, Yuming Zhao, Lihong Ge

“…GFP expression confirmed the engraftment of SHED in diabetic kidneys. In addition, cocultured SHED inhibited AGE-induced EMT in HK-2 cells. …”
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Intratumoural CD8+ CXCR5+ follicular cytotoxic T cells have prognostic value and are associated with CD19+ CD38+ B cells and tertiary lymphoid structures in colorectal cancer by Fangze Wei, Xiaotian Xu, Jing Wang, Shi Wen Mei, Fu Qiang Zhao, Fei Huang, Ti Xian Xiao, Guo Jing Wang, Baojun Wei, Shengkai Huang, Wei Cui

“…We also compared the biological functions of these cells between MSS and MSI-high CRC and used flow cytometry and coculture experiments to explore their potential regulatory functions. …”
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Macrophages facilitate tumor cell PD‐L1 expression via an IL‐1β‐centered loop to attenuate immune checkpoint blockade by Cheng Xu, Yu Xia, Bai‐Wei Zhang, Emmanuel Kwateng Drokow, Hua‐Yi Li, Sen Xu, Zhen Wang, Si‐Yuan Wang, Ping Jin, Tian Fang, Xiao‐Ming Xiong, Pu Huang, Ning Jin, Jia‐Hong Tan, Qing Zhong, Yu‐Xin Chen, Qi Zhang, Yong Fang, Fei Ye, Qing‐Lei Gao

“…Here, we revealed that interleukin (IL)‐1β was among the most abundant cytokines within the in vitro tumor‐macrophage coculture system, and enhanced IL‐1β expression was associated with impaired cytotoxicity of CD8+ T cells in human ovarian cancer, indicating the possibility that IL‐1β mediated immunosuppression during tumor‐TAMs crosstalk. …”
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Xixin Decoction’s novel mechanism for alleviating Alzheimer’s disease cognitive dysfunction by modulating amyloid-β transport across the blood–brain barrier to reduce neuroinflamma... by Chaokai Yang, Enlong Zhao, Hu Zhang, Liqi Duan, Xinyue Han, Hongli Ding, Yan Cheng, Dengkun Wang, Dengkun Wang, Dengkun Wang, Xiaojing Lei, Xiaojing Lei, Xiaojing Lei, Yongchang Diwu, Yongchang Diwu, Yongchang Diwu

“…This investigation sought to elucidate XXD’s impact on various aspects of AD pathology, including blood-brain barrier (BBB) impairment, neuroinflammatory processes, and amyloid-β (Aβ) deposition, as well as the molecular pathways involved in these effects.MethodsIn vitro experiments were conducted using hCMEC/D3 and HBVP cell coculture to establish an in vitro blood-brain barrier (BBB) model. …”
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MSCs Contribute to the Conversion of Ly6Chigh Monocytes into Ly6Clow Subsets under AMI by Wenbin Lu, Genshan Ma, Zulong Sheng, Qingjie Wang, Lijuan Chen, Junhua Qi, Ronghui Shi, Jingjing Ji, Zhenjun Ji, Qiming Dai

“…We first demonstrated that the percentage of Ly6Clow monocytes increased greatly after 3 days of coculture with MSCs (12.8%±3.77% vs. 3.69%±0.74%, p<0.001). …”
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Disrupting EDEM3‐induced M2‐like macrophage trafficking by glucose restriction overcomes resistance to PD‐1/PD‐L1 blockade by Shaoyong Peng, Minshan Wu, Qian Yan, Gaopo Xu, Yumo Xie, Guannan Tang, Jinxin Lin, Zixu Yuan, Xiaoxia Liang, Ze Yuan, Jingrong Weng, Liangliang Bai, Xiaolin Wang, Huichuan Yu, Meijin Huang, Yanxin Luo, Xiaoxia Liu

“…Methods Flow cytometric, Western blotting, proteomics analysis, analysis of public database data, genetically modified cell line models, T cell coculture, crystal violetstaining, ELISA, metabonomic and clinical tumour samples were conducted to assess the role of EDEM3 in immune escape and itsmolecular mechanisms. …”
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γδ T Cells’ Role in Donor-Specific Antibody Generation: Insights From Transplant Recipients and Experimental Models by Xavier Charmetant, Xavier Charmetant, Xavier Charmetant, Guillaume Rigault, Chien-Chia Chen, Hannah Kaminski, Hannah Kaminski, Jonathan Visentin, Jonathan Visentin, Benjamin Taton, Gabriel Marseres, Virginie Mathias, Alice Koenig, Alice Koenig, Alice Koenig, Thomas Barba, Thomas Barba, Thomas Barba, Pierre Merville, Pierre Merville, Stéphanie Graff-Dubois, Emmanuel Morelon, Emmanuel Morelon, Emmanuel Morelon, Julie Déchanet-Merville, Valérie Dubois, Jean-Paul Duong van Huyen, Lionel Couzi, Lionel Couzi, Olivier Thaunat, Olivier Thaunat, Olivier Thaunat

“…Analysis of a cohort of 331 kidney transplant recipients found no relation between the number of circulating γδ T cells and the risk to develop DSA. Coculture models demonstrated that activated γδ T cells were unable to promote the differentiation of B cells into plasma cells, ruling out that they can be “surrogate” TFH. …”
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Loop33 × 123 CAR-T targeting CD33 and CD123 against immune escape in acute myeloid leukemia by Haotian Ma, Zhifeng Yan, Runxia Gu, Yingxi Xu, Shaowei Qiu, Haiyan Xing, Kejing Tang, Zheng Tian, Qing Rao, Min Wang, Jianxiang Wang

“…Phenotype detection was evaluated on day 7 concerning activation, exhaustion, and subtype proportions. Coculture killing assays were conducted using various AML cell lines and primary AML cells. …”
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Deriving Human Intestinal Organoids with Functional Tissue-Resident Macrophages All From Pluripotent Stem CellsSummary by Kentaro Tominaga, Daniel O. Kechele, J. Guillermo Sanchez, Simon Vales, Ingrid Jurickova, Lizza Roman, Akihiro Asai, Jacob R. Enriquez, Heather A. McCauley, Keishi Kishimoto, Kentaro Iwasawa, Akaljot Singh, Yuko Horio, Jorge O. Múnera, Takanori Takebe, Aaron M. Zorn, Michael A. Helmrath, Lee A. Denson, James M. Wells

“…Following 2 weeks of coculture, the organoids were used for transcriptional profiling, functional analysis of macrophages, or transplanted into immunocompromised mice and matured in vivo for an additional 10–12 weeks. …”
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Targeting MYCN upregulates L1CAM tumor antigen in MYCN-dysregulated neuroblastoma to increase CAR T cell efficacy by Laura Grunewald, Lena Andersch, Konstantin Helmsauer, Silke Schwiebert, Anika Klaus, Anton G. Henssen, Teresa Straka, Marco Lodrini, Sebastian G. Wicha, Steffen Fuchs, Falk Hertwig, Frank Westermann, Alice Vitali, Carlotta Caramel, Gabriele Büchel, Martin Eilers, Kathy Astrahantseff, Angelika Eggert, Uta E. Höpken, Johannes H. Schulte, Thomas Blankenstein, Kathleen Anders, Annette Künkele

“…L1CAM-CAR T cell effector function was assessed (activation markers, cytokine release, tumor cytotoxicity) after coculture with the model or MYCN-amplified neuroblastoma cell lines. …”
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Effect of Prebiotics-Enhanced Probiotics on the Growth of Streptococcus mutans by Santichai Nunpan, Chatrudee Suwannachart, Kornchanok Wayakanon

“…The growth rate of the S. mutans significantly decreased when cocultured with L. acidophilus in the GOS-supplemented medium at 3%, 4%, and 5%. …”
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Effects of Placenta-Derived Mesenchymal Stem Cells on the Particulate Matter-Induced Damages in Human Middle Ear Epithelial Cells by So Young Kim, Seung Ha Oh, Jun Ho Lee, Myung-Whan Suh, Moo Kyun Park

“…The medium of PM-treated HMEECs cocultured with PL-MSCs exhibited increased PGE2 levels. …”
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Activated B Lymphocyte Inhibited the Osteoblastogenesis of Bone Mesenchymal Stem Cells by Notch Signaling by Mengxue Pan, Wei Hong, Ye Yao, Xiaoxue Gao, Yi Zhou, Guoxiang Fu, Yuanchuang Li, Qiang Guo, Xinxin Rao, Peiyuan Tang, Shengzhi Chen, Weifang Jin, Guoqiang Hua, Jianjun Gao, Xiaoya Xu

“…The osteogenesis of BMSCs cocultured with normal and LPS-pretreated B lymphocytes was detected by BCIP/NBT and Alizarin red S staining. …”
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Mesenchymal Stem Cells Pretreated with HGF and FGF4 Can Reduce Liver Fibrosis in Mice by Sulaiman Shams, Sadia Mohsin, Ghazanfar Ali Nasir, Mohsin Khan, Shaheen N. Khan

“…In vitro we gave CCl4 injury to mouse hepatocytes and cocultured it with untreated and cytokines pretreated MSCs. …”
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Understanding Cardiovascular Events With JAK Inhibitors: Tofacitinib Reduces Synovial and Vascular Inflammation but not the Prothrombotic Effects of Inflammatory Cytokines on Endot... by Aliki Zavoriti, Pierre Miossec

“…In cocultures with ECs, tofacitinib reduced further IL‐6 and IL‐8 production (both P < 0.001). …”
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Upregulated Expression of Indoleamine 2, 3-Dioxygenase in Primary Breast Cancer Correlates with Increase of Infiltrated Regulatory T Cells In Situ and Lymph Node Metastasis by Jinpu Yu, Jingyan Sun, Shizhen Emily Wang, Hui Li, Shui Cao, Yizi Cong, Juntian Liu, Xiubao Ren

“…We further demonstrated that CD4+CD25+CD127− Tregs could be amplified by coculturing CD3+ T cells with IDO/CHO cells in vitro which displayed increasing Foxp3 expression both at mRNA and protein levels. …”
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