Metformin Facilitates Osteoblastic Differentiation and M2 Macrophage Polarization by PI3K/AKT/mTOR Pathway in Human Umbilical Cord Mesenchymal Stem Cells by Min Shen, Huihui Yu, Yunfeng Jin, Jiahang Mo, Jingni Sui, Xiaohan Qian, Tong Chen

“…Mechanically, we found that metformin-treated M2 macrophages possessed more potent osteoinductive capacity in our coculture system. Molecularly, these metformin-stimulated M2 macrophages facilitated osteogenesis via activating the PI3K/AKT/mTOR pathway. …”
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Kupffer Cells Promote the Differentiation of Adult Liver Hematopoietic Stem and Progenitor Cells into Lymphocytes via ICAM-1 and LFA-1 Interaction by Deping Meng, Yuhong Qin, Nan Lu, Keke Fang, Yuan Hu, Zhigang Tian, Cai Zhang

“…A blockade of intercellular cell adhesion molecule-1 (ICAM-1) in a liver HSPC and kupffer cell coculture system impaired the adhesion, expansion, and differentiation of HSPCs. …”
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Hair Follicle Dermal Cells Support Expansion of Murine and Human Embryonic and Induced Pluripotent Stem Cells and Promote Haematopoiesis in Mouse Cultures by Jun Liu, Claire A. Higgins, Jenna C. Whitehouse, Susan J. Harris, Heather Crawford, Angela M. Christiano, Majlinda Lako, Nicholas Hole, Colin A. B. Jahoda

“…Finally, we show that cultured mouse follicle dermal cells provide good stromal support for haematopoiesis in an established coculture model. Human follicular dermal cells represent an accessible and readily propagated source of feeder cells for pluripotent and haematopoietic cells and have potential for use in clinical applications.…”
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Enhancement of Anti-Inflammatory and Osteogenic Abilities of Mesenchymal Stem Cells via Cell-to-Cell Adhesion to Periodontal Ligament-Derived Fibroblasts by Keita Suzuki, Naoyuki Chosa, Shunsuke Sawada, Naoki Takizawa, Takashi Yaegashi, Akira Ishisaki

“…Expression levels of anti-inflammatory and inflammatory cytokines were increased and decreased, respectively, by direct-contact coculture between MSCs and PDL-Fs. In addition, the direct-contact coculture enhanced the expression of MSC markers that play important roles in the self-renewal and maintenance of multipotency of MSCs, which in turn induced the osteogenic ability of the cells. …”
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Mouse Mesenchymal Progenitor Cells Expressing Adipogenic and Osteogenic Transcription Factors Suppress the Macrophage Inflammatory Response by Natalie Fernandez, Heather Renna, Lauren McHugh, Katie Mazolkova, William Crugnola, Jodi F. Evans

“…Their interaction and regulation of the MΦ cell response to the inflammatory mediator, lipopolysaccharide (LPS), was examined by coculture. As expected, D1 cells suppressed NO, TNF-α, and IL-12p70 production but MΦ phagocytic activity remained unchanged. …”
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Curcumin Improves Epithelial Barrier Integrity of Caco-2 Monolayers by Inhibiting Endoplasmic Reticulum Stress and Subsequent Apoptosis by Xinxin Zhou, Mengting Ren, Jinpu Yang, Hanghai Pan, Mosang Yu, Feng Ji

“…Although some studies have reported the therapeutic effects of curcumin on ulcerative colitis (UC), the specific mechanism remains unclear. An in vitro coculture model of Caco-2 and differentiated THP-1 cells was established. …”
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Exposure of Mesenchymal Stem Cells to an Alzheimer’s Disease Environment Enhances Therapeutic Effects by Sang Eon Park, Hyeong Seop Kim, Soo Jin Kwon, Min-Jeong Kim, Suk-joo Choi, Soo-young Oh, Gyu Ha Ryu, Hong Bae Jeon, Duk L. Na, Jong Wook Chang

“…Previous studies suggested that the coculture of human MSCs with AD in an in vitro model reduced the expression of amyloid-beta 42 (Aβ42) in the medium as well as the overexpression of amyloid-beta- (Aβ-) degrading enzymes such as neprilysin (NEP). …”
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Amoeba plate test with Acanthamoeba castellanii as an innovative tool for Nocardia recovery from sputum samples: a proof-of-concept study by Coralie Espinasse, Ghislaine Descours, Marine Ibranosyan, Laetitia Beraud, Christophe Ginevra, Joelle Chastang, Oana Dumitrescu, Frédéric Laurent, Verónica Rodriguez Nava, Sophie Jarraud, Camille Allam

“…This study aimed to compare the amoeba plate test (APT), an amoebic coculture with Acanthamoeba castellanii, with the axenic culture to recover Nocardia from pulmonary samples. …”
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Beneficial Effects of Human Mesenchymal Stromal Cells on Porcine Hepatocyte Viability and Albumin Secretion by Elisa Montanari, Joel Pimenta, Luca Szabó, François Noverraz, Solène Passemard, Raphael P. H. Meier, Jeremy Meyer, Jonathan Sidibe, Aurelien Thomas, Henk-Jan Schuurman, Sandrine Gerber-Lemaire, Carmen Gonelle-Gispert, Leo H. Buhler

“…We further investigated whether coculture and coencapsulation of hepatocytes with human multipotent mesenchymal stromal cells (MSC) are beneficial on hepatocyte function. …”
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miR663 Prevents Epo Inhibition Caused by TNF-Alpha in Normoxia and Hypoxia by Mete Ozkurt, Thomas Hellwig-Bürgel, Reinhard Depping, Selda Kadabere, Rumeysa Ozyurt, Abdullah Karadag, Nilüfer Erkasap

“…In our coculture study, transfecting HepG2 cells with miR663 mimics decreased the apoptosis of UT-7 cells.…”
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Alteration in the Cytokine Secretion of Bone Marrow Stromal Cells from Patients with Chronic Myelomonocytic Leukemia Contribute to Impaired Hematopoietic Supportive Activity by Hui Shi, Yingshao Wang, Rong Li, Wen Xing, Feng-Chun Yang, Jie Bai, Yuan Zhou

“…We showed that noncontacted coculture with CMML-BMSCs preferentially promoted the differentiation of CB CD34+ cells toward myeloid lineage. …”
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Mesenchymal Stromal Cells from Osteoarthritic Synovium Are a Distinct Population Compared to Their Bone-Marrow Counterparts regarding Surface Marker Distribution and Immunomodulati... by Sebastien Hagmann, Claudia Rimmele, Florin Bucur, Thomas Dreher, Felix Zeifang, Babak Moradi, Tobias Gotterbarm

“…Based on previous studies in an allogeneic T-cell coculture model, we aimed at further determining the role of synovial MSCs in OA pathogenesis. …”
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El género, la fábrica y la vida urbana en la frontera by Marlene Solís Pérez

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Method for determining of cytotoxicity based on the release of fluorescent proteins by Dmitry Lifanov, Dulamsuren Zorigt, Evgenya Shabalina, Abdullah Khalil, Konstantin Gorbunov, Elena Petersen

“…We included liver cells (Huh7) in a coculture model and determined changes in the cytotoxicity levels of capecitabine against NCI-H1299 cells. …”
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Binding of Host Factors Influences Internalization and Intracellular Trafficking of Streptococcus uberis in Bovine Mammary Epithelial Cells by Raul A. Almeida, John R. Dunlap, Stephen P. Oliver

“…Thus, experiments involving coculture of collagen-, fibronectin-, and LF-pretreated S. uberis with bovine mammary epithelial cells treated with RME and CME inhibitors were conducted. …”
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Differential vasoproliferative traits of Bartonella henselae strains associated with autotransporter BafA variants by Yuka Kondo, Masahiro Suzuki, Shingo Sato, Soichi Maruyama, Akiko Sei, Xingyan Ma, Kota Nakano, Yohei Doi, Kentaro Tsukamoto

“…However, BafA variant 2 strains showed cytotoxicity at a high bacterial inoculum in a direct coculture with HUVECs, which was attenuated in an indirect coculture. …”
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Monitoring Steady Flow Effects on Cell Distribution in Engineered Valve Tissues by Magnetic Resonance Imaging by Catalina Martinez, Angela Henao, Jose E. Rodriguez, Kyle R. Padgett, Sharan Ramaswamy

“…When physiologically representative shear stresses (5–6 dynes/cm 2 ) were applied to SMC-EC coculture–seeded scaffolds, hypointense regions seemed to have decreased after 2 weeks in some locations, whereas others revealed sustained levels of T 2 * contrast; similar observations were seen in the case of BMSC-seeded scaffolds. …”
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Adhesion-independent synergy of monocytes and endothelial cells in cytokine production: regulation of IL-6 and GM–CSF production by PAF by C. Lacasse, S. Turcotte, D. Gingras, M. Rola-Pleszczynski

“…In contrast, GM–CSF production was not enhanced by coculture of monocytes with activated EC. When either cell population was fixed with paraformaldehyde or killed by freeze-thawing before addition to the co-culture, cytokine levels reverted to those produced by the unaffected population alone. …”
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Stable Agrobacterium-Mediated Transformation of Maritime Pine Based on Kanamycin Selection by José M. Alvarez, Ricardo J. Ordás

“…Different factors, such as embryogenic line, bacterial strain, bacterial concentration, and coculture duration, were examined and optimized. For selection of transformants, 15 mgL−1 kanamycin was used. …”
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The Regulation between CD4+CXCR5+ Follicular Helper T (Tfh) Cells and CD19+CD24hiCD38hi Regulatory B (Breg) Cells in Gastric Cancer by Ying Zhang, Jun Wu, Haibo Zhang, Chen Wu

“…The in vitro Tfh-B cell coculture experiments were performed for six days, and then, Tfh%, Breg%, and cytokines were valued by flow cytometry and ELISA, respectively. …”
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