Construction and Characterization of Adenovirus Vectors Encoding Aspartate-β-Hydroxylase to Preliminary Application in Immunotherapy of Hepatocellular Carcinoma by Yujiao Zhou, Feifei Liu, Chengmin Li, Guo Shi, Xiaolei Xu, Xue Luo, Yuanling Zhang, Jingjie Fu, Aizhong Zeng, Limin Chen

“…A dramatically cell-killing effect of T lymphocytes coculturing with AAH-DCs on HepG2 HCC cell line was demonstrated by CCK-8 and FCM assays in vitro. …”
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Anti-IL-22 Antibody Attenuates Acute Graft-versus-Host Disease via Increasing Foxp3+ T Cell through Modulation of CD11b+ Cell Function by Jianbo Wu, Jian Gu, Shun Zhou, Hao Lu, Yunjie Lu, Ling Lu, Xuehao Wang

“…In vitro Treg induction was more efficient when CD4+CD25− T cells differentiated in the presence of CD11b+ cells obtained from IL-22Ab-treated GVHD mice, compared with cocultured untreated control cells. Finally, IL-22Ab modulated the expression of cytokines and costimulatory molecules in CD11b+ cells in aGVHD mice. …”
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Islet Transplantation Reverses Podocyte Injury in Diabetic Nephropathy or Induced by High Glucose via Inhibiting RhoA/ROCK/NF-κB Signaling Pathway by Chongchu Huang, Yi Zhou, Hongjian Huang, Yushu Zheng, Lijun Kong, Hewei Zhang, Yan Zhang, Hongwei Wang, Mei Yang, Xiaona Xu, Bicheng Chen

“…Islet cells, CCG-1423 (RhoA Inhibitor), and fasudil (ROCK inhibitor) were then cocultured with podocytes in vitro to assess their protective effects on podocyte injury induced by high glucose (HG). …”
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Comparison of global transcriptomes for nontyphoidal Salmonella clinical isolates from pediatric patients with and without bacteremia after their interaction with human intestinal... by Buyandelger Batsaikhan, Pei-Chun Lin, Katsumi Shigemura, Yu-Wei Wu, Reo Onishi, Pei-Ru Chang, Hung-Yen Cheng, Shiuh-Bin Fang

“…The bacterial RNA samples were isolated after coculturing with Caco-2 cells for RNA sequencing and subsequent analyses. …”
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Exosomes derived from syncytia induced by SARS-2-S promote the proliferation and metastasis of hepatocellular carcinoma cells by Huilong Li, Huilong Li, Haotian Lin, Haotian Lin, Tinghui Fan, Linfei Huang, Li Zhou, Xiaoyu Tian, Ruzhou Zhao, Yanhong Zhang, Xiaopan Yang, Luming Wan, Hui Zhong, Nan Jiang, Congwen Wei, Wei Chen, Wei Chen, Lihua Hou, Lihua Hou

“…However, the molecular mechanism for the increased mortality in cancer patients infected with COVID-19 is unclear.MethodsColony formation and wound healing assays were performed on Huh-7 cells cocultured with syncytia. Exosomes were purified from the cell supernatant and verified by nanoparticle tracking analysis (NTA), Western blot (WB) analysis and scanning electron microscopy (SEM). …”
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TLR4/AP-1-Targeted Anti-Inflammatory Intervention Attenuates Insulin Sensitivity and Liver Steatosis by Xiang Hu, Jing Zhou, Sha-sha Song, Wen Kong, Yan-Chuan Shi, Lu-Lu Chen, Tian-Shu Zeng

“…Additionally, TLR4/AP-1 siRNA transfection mitigated palmitic acid- (PA-) induced inflammation in RAW264.7 cells and metabolic abnormalities in cocultured AML hepatocytes. Herein, we propose that TLR4-AP1 signaling pathway activation plays a crucial role in high fat- or PA-induced metabolic inflammation and insulin resistance in hepatocytes. …”
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Mesenchymal Stromal Cells Accelerate Epithelial Tight Junction Assembly via the AMP-Activated Protein Kinase Pathway, Independently of Liver Kinase B1 by P. Rowart, P. Erpicum, J.-M. Krzesinski, M. Sebbagh, F. Jouret

“…The in vitro Ca2+ switch from 5 μM to 1.8 mM was performed using epithelial Madin-Darby canine kidney (MDCK) cells cultured alone or cocultured with rat bone marrow-derived MSC or preexposed to MSC-conditioned medium. …”
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Ethanol Extracts of Solanum lyratum Thunb Regulate Ovarian Cancer Cell Proliferation, Apoptosis, and Epithelial-to-Mesenchymal Transition (EMT) via the ROS-Mediated p53 Pathway by Chen Zhang, Zheming Li, Jie Wang, Xuelu Jiang, Mengting Xia, Jianfen Wang, Shenyi Lu, Shouye Li, Hanmei Wang

“…Furthermore, A2780 and SKOV3 cells cocultured with M0 macrophages showed that SLT activated the polarization of M0 macrophages to M1 macrophages and inhibited the polarization to M2 macrophages, with the increased percentage of CD86+ cells and decreased percentage of CD206+ cells were detected. …”
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The Possible Role of Neutrophils in the Induction of Osteoclastogenesis by Carolyn G. J. Moonen, Teun J. de Vries, Patrick Rijkschroeff, Patrice E. Poubelle, Elena A. Nicu, Bruno G. Loos

“…These limited levels of expression did not induce osteoclastogenesis when cocultured with preosteoclasts for 10 days. We report that, under the aforementioned experimental conditions, neither cPMNs nor oPMNs directly induced osteoclastogenesis. …”
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SDF-1α/OPF/BP Composites Enhance the Migrating and Osteogenic Abilities of Mesenchymal Stem Cells by Linli Li, Xifeng Liu, Bipin Gaihre, Sungjo Park, Yong Li, Andre Terzic, Lichun Lu

“…SDF-1α/OPF/BP composites were loaded with escalating SDF-1α concentrations, namely, 0 ng/ml, 50 ng/ml, 100 ng/ml, and 200 ng/ml, and were cocultured with MSC. Scratching assay, Transwell assay, and three-dimensional migration model were utilized to assess the migration response of MSCs. …”
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Caveolin-1 Knockdown Decreases SMMC7721 Human Hepatocellular Carcinoma Cell Invasiveness by Inhibiting Vascular Endothelial Growth Factor-Induced Angiogenesis by Zhi-Bo Zhang, Zheng Shi, Lan-Fang Yang, Hong-Bin Gao

“…Phospho-eNOS levels in human umbilical vein endothelial cells (HUVECs) cocultured with SMMC7721 cell supernatants were analyzed by Western blot. …”
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The Effects of Immunosuppressive Factors on Primary Dendritic Cells from C57BL/6 and CBA Mice by Vasiliy V. Kurilin, Julia N. Khantakova, Valeriy P. Tereschenko, Julia A. Lopatnikova, Irina A. Obleukhova, Sergey V. Sennikov

“…DCs were then treated with different types of immunosuppressive molecules (rmIL-10, rmTGF-β, and BAY 11-7082) and cocultured with syngeneic splenocytes. The amount of CD4+CD25hiFoxP3+ Tregs, IL-10 expression, and proliferation were evaluated. …”
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Tasquinimod suppresses tumor cell growth and bone resorption by targeting immunosuppressive myeloid cells and inhibiting c-MYC expression in multiple myeloma by Marie Törngren, Rong Fan, Eline Menu, Helena Eriksson, Karin Vanderkerken, Andrew Chantry, Hatice Satilmis, Niels Vandewalle, Emma Verheye, Philip Vlummens, Anke Maes, Catharina Muylaert, Elke De Bruyne, Holly Evans, Nathan De Beule, Dirk Hose, Ken Maes, Karine Breckpot, Kim De Veirman

“…Myeloid cells and T cells were cocultured at different ratios to assess tasquinimod-mediated immunomodulatory effects. …”
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Infection of nonclassic monocytes by respiratory syncytial virus induces an imbalance in the CD4+ T-cell subset response by Lianlian Han, Danyang Li, Conghui Wang, Lili Ren, Li Guo, Jianwei Wang

“…We found that RSV efficiently infects CD16+ monocytes, but not CD16- monocytes, via cocultures of CD4+ T-cells with RSV-infected CD16+ monocytes, inhibits Treg cell proliferation and increases Th2 cell frequency, suggesting that RSV causes an imbalance in the CD4+ T-cell subset by primarily infecting CD16+ monocytes. …”
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Human Umbilical Cord Mesenchymal Stem Cells Attenuate Ocular Hypertension-Induced Retinal Neuroinflammation via Toll-Like Receptor 4 Pathway by Shangli Ji, Jie Xiao, Jian Liu, Shibo Tang

“…In addition, via in vitro experiments, we found that the increased expression of the TLR4 signaling pathway induced by lipopolysaccharide (LPS) was markedly decreased after hUC-MSCs were cocultured with rMC-1 and BV2 cells. These findings indicate that hUC-MSC transplantation attenuates OHT-induced retinal neuroinflammation via the TLR4 pathway.…”
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Exosomes from LPS-Stimulated hDPSCs Activated the Angiogenic Potential of HUVECs In Vitro by Xiangyu Huang, Wei Qiu, Yuhua Pan, Jianjia Li, Zhao Chen, Kaiying Zhang, Yifei Luo, Buling Wu, Wenan Xu

“…Exosomes were isolated from hDPSCs with or without LPS stimulation (N-EXO and LPS-EXO) and cocultured with human umbilical vein endothelial cells (HUVECs). …”
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Pharmacological Preconditioning Improves the Viability and Proangiogenic Paracrine Function of Hydrogel-Encapsulated Mesenchymal Stromal Cells by Francesco K. Touani, Melanie Borie, Feryel Azzi, Dominique Trudel, Nicolas Noiseux, Shant Der Sarkissian, Sophie Lerouge

“…Paracrine function was studied first by quantifying the proangiogenic growth factors released, followed by assessing scratched HUVEC culture wound closure velocity and proliferation of HUVEC when cocultured with encapsulated hMSC. In vivo, the proangiogenic activity was studied by evaluating the neovessel density around the subcutaneously injected hydrogel after one week in rats. …”
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Effect of TNF-α-Induced Sclerostin on Osteocytes during Orthodontic Tooth Movement by Fumitoshi Ohori, Hideki Kitaura, Aseel Marahleh, Akiko Kishikawa, Saika Ogawa, Jiawei Qi, Wei-Ren Shen, Takahiro Noguchi, Yasuhiko Nara, Itaru Mizoguchi

“…Moreover, the number of TRAP-positive cells was increased in osteocytes and osteoclast precursors cocultured with sclerostin. In vivo analysis of mouse calvariae that had been subcutaneously injected with phosphate-buffered saline (PBS) or TNF-α revealed that the number of TRAP-positive cells and the percentage of sclerostin-positive osteocytes were higher in the TNF-α group than in the PBS group. …”
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Hypoxia-Elicited Mesenchymal Stem Cell-Derived Small Extracellular Vesicles Alleviate Myocardial Infarction by Promoting Angiogenesis through the miR-214/Sufu Pathway by Lianbo Shao, Yihuan Chen, Jingjing Li, Jingfan Chao, Ziying Yang, Yinglong Ding, Han Shen, Yueqiu Chen, Zhenya Shen

“…Scratch assays and tube formation assays were performed in sEVs cocultured with HUVECs to assess the proangiogenic capability of MSC-sEVs and MSChyp-sEVs. …”
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Screening and characterization of the scFv for chimeric antigen receptor T cells targeting CEA-positive carcinoma by Chengcheng Zhang, Linling Wang, Qianzhen Zhang, Junjie Shen, Xia Huang, Meiling Wang, Yi Huang, Jun Chen, Yanmin Xu, Wenxu Zhao, Yanan Qi, Yunyan Li, Yanjiao Ou, Zhi Yang, Cheng Qian

“…M5A, hMN-14, and BW431/26 CAR-T cells exhibited effective tumor cell lysis and IFN-γ release when cocultured with CEA-positive tumor cells in vitro, correlating with the abundance of CEA expression in target cells. …”
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