A nucleoside-modified mRNA vaccine prevents enterovirus A71 infection in mouse model by Fengyu Chi, Fengyu Chi, Xu Zhang, Xu Zhang, Dong Zhang, Dong Zhang, Airu Zhu, Zhen Zhuang, Zhaoyong Zhang, Zhenjie Zhang, Chuansong Quan, Kaixiao Nie, Juan Li, Chunhong Yin, Jie Tong, Yuming Li, Yuming Li, Yuming Li

“…Immunogenicity and protective efficacy were evaluated in BALB/c and neonatal A129 mice, respectively. Immune responses were assessed by ELISA, micro-neutralization assays, ELISpot, and intracellular cytokine staining (ICS). …”
Get full text

食管癌细胞核仁抗原单克隆抗体的制备 及其特性的初步鉴定 by 刘乐和, 沈忠英

“…【方法】以食管癌组 织核基质为抗原,采用足垫法免疫 Balb/c 小鼠,运用杂交瘤技术建立分泌特异性单抗的杂交瘤细胞株, 制备食管癌细胞抗原 片和食管癌冰冻切片,采用免疫组化检测并采用蛋白印迹法(Western blotting)分析, 鉴定是否为特异性单克隆抗体。…”
Get full text

Genetic diversity leads to differential inflammatory responses to cigarette smoke in mice by Md Imam Faizan, Gagandeep Kaur, Sadiya Bi Shaikh, Felix Effah, Hoshang Unwalla, Irfan Rahman

“…The study involves a comprehensive approach, including BALF cytokine analysis, evaluation of lung tissue architecture, assessment of macrophages and its associated proteins (MMP9 & MMP12) abundance. …”
Get full text

A molecular toxicological study to explore potential health risks associated with ultrafine particle exposure in cold and humid indoor environments by Ziyu Shu, Shuo Qing, Xu Yang, Ping Ma, Yang Wu, Baizhan Li, Fangxin Fang, Runming Yao

Subjects: Get full text

Antioxidant, Antimicrobial, and Protein Kinase Inhibition Profiling of C. ambrosioides Seed Extracts along with RP-HPLC by Saira Bano, Muhammad Waleed Baig, Mohammad K. Okla, Syeda Saniya Zahra, Nosheen Akhtar, Wahidah H. Al-Qahtani, Hamada AbdElgawad, Ihsan-Ul Haq

“…A noteworthy protein kinase inhibitory potential was shown by ethanol extract with a 20±1.27 mm bald zone. Therapeutic potential of medicinal plants can be completely explored by using multiple solvent system. …”
Get full text

Macrofilaricidal Activity, Acute and Biochemical Effects of Three Lichen Species Found on Mount Cameroon by Thierry Roland Kang, Jerome Nyhalah Dinga, Ayuk Elizabeth Orock, Elvis Monya, Moses Njutain Ngemenya

“…Toxicity of highly active extracts was investigated on monkey kidney epithelial (LLCMK2) cells and in BALB/c mice (2000 mg/kg body weight) including effects on liver enzymes. …”
Get full text

胰岛培养后胸腺内移植的实验研究 by 王国栋, 陈规划

“…【目的】观察胰岛培养后胸腺内移植对移植物存活时间的影响。【方法】以C57BL/6 小鼠为受体, BALB/c 小鼠为 供体。胸腺内胰岛未培养组分为单纯移植和移植的同时加腹腔内1 次性注射兔抗小鼠胸腺细胞血清(ATS)两组。…”
Get full text

Otodectes cynotis (Acari: Psoroptidae) infestations in Southern pudus (Pudu puda): In situ and ex situ data of an unexpected host-parasite record by Caroline Wilhelm, Edwin Kniha, Pamela Muñoz, Ángelo Espinoza, Laura Platner, Saskia Dreyer, Lisa Grund, Zoë Tess Lara Lindhorst, Ulrich Gärtner, Julia Walochnik, Anja Taubert, Dominik Fischer, Stephan Hering-Hagenbeck, Carlos Hermosilla, David Ebmer

“…Otodectes cynotis (Acari: Psoroptidae) constitutes an obligate, non-burrowing ectoparasite and causes otodectic mange primarily in domestic and wild carnivores. …”
Get full text

Effect of Black Cumin on the Expression of Genes Related to Lipid Metabolism in Adipose Tissue: A Mouse Model Study by Roya Maleki, Ali Ahadi, Hoda Ayat

“…This study aimed to investigate the effect of black cumin on the expression of some important genes in the metabolism of lipids. For this purpose, 14 male Balb/c mice were divided into control and treatment groups. …”
Get full text

15-Deoxy-γ12,14-prostaglandin J2 Reduces Liver Impairment in a Model of ConA-Induced Acute Hepatic Inflammation by Activation of PPARγ and Reduction in NF-κB Activity by Kan Chen, Jingjing Li, Junshan Wang, Yujing Xia, Weiqi Dai, Fan Wang, Miao Shen, Ping Cheng, Yan Zhang, Chengfen Wang, Jing Yang, Rong Zhu, Huawei Zhang, Yuanyuan Zheng, Jie Lu, Zhuoyi Fan, Yingqun Zhou, Chuanyong Guo

“…The histological grade, proinflammatory cytokine levels, and NF-κB and PPARγ activity were determined 6, 12, and 24 h after the ConA injection. In vitro, LO2 cells and RAW264.7 cells were pretreated with 15d-PGJ2 (2 μM) 1 h before the stimulation with ConA (30 μg/mL). …”
Get full text

Genotype-Dependent Difference in 5-HT2C Receptor-Induced Hypolocomotion: Comparison with 5-HT2A Receptor Functional Activity by Darya V. Bazovkina, Elena M. Kondaurova, Vladimir S. Naumenko, Evgeni Ponimaskin

“…These experiments revealed significant positive correlation between 5-HT2C and 5-HT2A receptors functional responses for all investigated mouse strains. Moreover, we found that 5-HT2A receptor activation with DOI did not change locomotor activity in CBA/Lac mice. …”
Get full text

mRNA vaccines with RBD mutations have broad-spectrum activity against SARS-CoV-2 variants in mice by Xiaoming Liang, Yuxia Yuan, Junbin Wang, Cong Tang, Yun Yang, Yanan Zhou, Hao Yang, Qing Huang, Wenhai Yu, Haixuan Wang, Yuhuan Yan, Dongdong Lin, Yanwen Li, Xuena Du, Longhai Yuan, Wenqi Quan, Daoju Wu, Shuaiyao Lu

“…Both vaccines elicited a robust immune response in BALB/c and K18-hACE2 mice, generating high levels of specific binding antibodies against the BA.2 RBD. …”
Get full text

恶性疟原虫 FCC-1/HN 株 DNA 疫苗 在小鼠体内的免疫反应 by 吕芳丽, 余新炳, 郭　虹, 陈观今

“…【方法】将恶性疟原虫 FCC-1/HN 株重组质粒 pc DNA3-Pfs25 及 pcDNA3-EBA175/HRP Ⅱ经骨骼肌途径分别单独注射或两者混合注射免疫 BALB/c 小鼠, 观察免疫后不同时 间点血清中 IgG 抗体滴度、脾淋巴细胞增殖反应、CD4+/CD8+ T 细胞亚群比值和 NK 细胞杀伤活性的变化。…”
Get full text

A pan-immune panorama of bacterial pneumonia revealed by a large-scale single-cell transcriptome atlas by Kun Xiao, Yan Cao, Zhihai Han, Yuxiang Zhang, Laurence Don Wai Luu, Liang Chen, Peng Yan, Wei Chen, Jiaxing Wang, Ying Liang, Xin Shi, Xiuli Wang, Fan Wang, Ye Hu, Zhengjun Wen, Yong Chen, Yuwei Yang, Haotian Yu, Lixin Xie, Yi Wang

“…Here, we employed single-cell RNA sequencing of 444,146 bronchoalveolar lavage fluid cells (BALFs) from a large cohort of 74 individuals, including 58 patients with mild (n = 22) and severe (n = 36) diseases as well as 16 healthy donors. …”
Get full text

Dual Mechanisms of Action: Anti-Candida and Anti-Inflammatory Potential of <i>Lactobacillus</i> Fermentation Broth in Treating Vulvovaginal Candidiasis by Huann-Cheng Horng, Jin-Wei Xu, Yi-Shan Kuo, Yu-Sin Chen, Yu-Hsuan Chiu, Kuan-Hao Tsui, Yu-Tang Tung

“…Vulvovaginal candidiasis (VVC), a condition predominantly caused by <i>Candida albicans</i>, affects millions of women worldwide, prompting the need for alternative treatments due to the side effects and increasing resistance associated with conventional imidazole antifungals. …”
Get full text

NCAPD3‐mediated AKT activation regulates prostate cancer progression by Yi Zhang, Wanlin Xie, Xicui Zong, Yuanyuan Fang, Jia Ren, Zuolei Jing, Yong Wei, Shan Lu, Qingyi Zhu, Ping Liu

“…CCK8, transwell, and wound‐healing assays were applied to perform cell proliferation and migration. A subcutaneous tumor xenograft model was constructed by injecting DU145‐Lv‐NCAPD3 cells and control cells into male BALB/c nude mice to confirm the result derived from in vitro assay. …”
Get full text

Corynebacteria from the respiratory microbiota modulate inflammatory responses and associate with a reduced pneumococcal burden in the lungs by Caroline Bergenfelz, Phuong Do, Liv Larsson, Hanna Ivarsson, Kasper Malmborn, Anders P. Håkansson

“…The specific role of Corynebacteria during respiratory health and disease is, however, largely uncharacterized.MethodRespiratory tract epithelial cells NCI-H292 and BALB/cByJ mice were inoculated with Corynebacteria (C. propinquum 2018M3 and 2019M4, and C. pseudodiphtheriticum 2019M8 and 2020M12) alone or with subsequent challenge with Streptococcus pneumoniae (pneumococci). …”
Get full text

Exposure to Air Pollution Exacerbates Inflammation in Rats with Preexisting COPD by Jing Wang, Ya Li, Peng Zhao, Yange Tian, Xuefang Liu, Huihui He, Rui Jia, Brian G. Oliver, Jiansheng Li

“…PM2.5 also aggravated inflammation, increasing neutrophils and eosinophils in bronchoalveolar lavage fluid (BALF) and cytokines including Interleukin- (IL-) 1β, granulocyte-macrophage colony-stimulating factor (GM-CSF), and IL-4. …”
Get full text

Intranasal Immunization with DNA Vaccine HA-CCL19/Polyethylenimine/Chitosan Composite Provides Immune Protection Against H7N9 Infection by Yuqing Xiang, Hongbo Zhang, Youcai An, Ze Chen

Subjects: Get full text

Preservation of cfRNA in cytological supernatants for cfDNA & cfRNA double detection in non‐small cell lung cancer patients by Yidan Ma, Yifei Wang, Lei He, Jun Du, Lin Li, Zhixin Bie, Yuanming Li, Xiaomao Xu, Wei Zhou, Xiaonan Wu, Li Yang, Jing Di, Chenyang Li, Xiaoguang Li, Dongge Liu, Zheng Wang

Subjects: “…cell‐free RNA…”
Get full text