Development a glycosylated extracellular vesicle-derived miRNA Signature for early detection of esophageal squamous cell carcinoma by Jianlin Chen, Yue Zheng, Zhen Wang, Qi Gao, Kun Hao, Xiongfeng Chen, Nantian Ke, Xiang Lv, Jiamiao Weng, Yuhong Zhong, Zhixin Huang, Miao Fu, Lilan Zhao, Fan Lin, Hui Mi, Haijun Tang, Chundong Yu, Yi Huang

“…Fucosylated (Fuc-) and sialylated (Sia-) EVs were isolated utilizing Lentil lectin (LCA) and wheat germ lectin (WGA)-coated magnetic beads, respectively. The glycosylated EVs-derived miRNAs-based signature (RiskscoreFuc−&Sia−) was established through logistic regression in a training cohort and subsequently validated in an internal and an external multicentre cohort. …”
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Thematic Curriculum and Academic Performance of Pupils in Upper Primary Classes: A Case of Selected Primary Schools in Kamwezi Sub-County Rukiga District. by Natukunda, Millia

“…Finally, the following recommendations were made; there is a need for the government to recruit more thematic teachers to handle large thematic classes. thematic teachers should use child-centered methods of teaching like demonstration, group discussion, and guided discovery where all pupils have a practical approach to learning and can arouse pupils' interest bead teachers should ensure that there is a regular supervisory system in their schools to improve teaching methods and social background of pupils.…”
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Association between Polyunsaturated Fatty Acid Profile and Bronchial Inflammation in Bronchiolitis Obliterans by Silvija P. Jerkic, Laura Bächle, Ruth Pia Duecker, Lucia Gronau, Andreas G. Chiocchetti, Stefan Zielen, Ralf Schubert

“…Induced sputum was analyzed for cytology and cytokine levels (IL-1ß, IL-6, IL-8, TNF-α) using cytometric bead array (CBA). The PUFA profile was determined in the serum and induced sputum by gas chromatography. …”
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lncRNA FR215775 Regulates Th2 Differentiation in Murine Allergic Rhinitis by Yue Ma, Le Shi, Keqing Zhao, Chunquan Zheng

“…After knocking down the expression of lncRNA FR215775 via Sh-FR215775-Ads, Cell Counting Kit-8, cytometric bead array, and fluorescence-activated cell sorting were performed to determine its functions in vitro. …”
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Identification of novel autoantibodies in Sjögren’s disease by Fiona Engelke, Petra Budde, Salvatore De Vita, Thomas Dörner, Diana Ernst, Jan Gras, Harald Heidecke, Annika Loredana Kilian, Katja Kniesch, Ann-Sophie Lindemann, Luca Quartuccio, Jacob Ritter, Kai Schulze-Forster, Benjamin Seeliger, Hans-Dieter Zucht, Torsten Witte

“…We aimed to identify novel autoantibodies in SjD that may facilitate the diagnostic procedure for Ro/SSA negative SjD.MethodsIgG and IgA autoantibody reactivity of 94 potential candidate autoantigens for SjD, selected from a discovery screen of 1,629 human antigens coupled to Luminex beads and prior knowledge about potential biological relevance, were examined in serum of SjD patients (n=347) using Luminex and ELISA technology. …”
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Cost-effectiveness of vector control for supplementing mass drug administration for eliminating lymphatic filariasis in India. by Donald S Shepard, Aung K Lwin, Sunish I Pulikkottil, Mariapillai Kalimuthu, Natarajan Arunachalam, Brij K Tyagi, Graham B White

“…The arms were: MDA alone (the standard of care); MDA plus VCS (expanded polystyrene beads covering the water surface in wells and cesspits to suppress the filariasis vector mosquito Culex quinquefasciatus); and MDA plus VCI (VCS plus insecticidal pyrethroid-impregnated curtains [over windows, doors, and eaves). …”
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Evaluation of de novo donor specific antibodies after kidney transplantation in the era of donor-derived cell-free DNA by Yuan Tian, Lukas Frischknecht, Anna Mallone, Fabian Rössler, Thomas Schachtner, Jakob Nilsson

“…While de novo donor-specific antibodies (dnDSA) are crucial in ABMR, the relationship between dd-cfDNA levels and dnDSA features, such as DSA category, MFI and HLA target loci, remains unclear.MethodsWe analyzed dd-cfDNA levels in 75 kidney transplant recipients who developed dnDSA post-transplant. dnDSA were categorized as “true”, “possible”, or “false” based on bead reactivity patterns and HLA typing. dd-cfDNA was assessed alongside dnDSA detection and sequential follow-up samples in a subgroup.Results“True” dnDSA showed significantly higher dd-cfDNA levels compared to “possible” and “false” groups. …”
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Leveraging the fundamentals of heat transfer and fluid mechanics in microscale geometries for automated next-generation sequencing library preparation by Olivia Ott, Sabrina Tolppi, Jennifer Figueroa-Cruz, Khaliun Myagmar, Khulan Unurbuyan, Anubhav Tripathi

“…Automatic reagent movement, mixing, and magnetic bead-based washing with capillary-based thermal cycling (capillary-PCR) are completely integrated into a single platform. …”
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Th9 Cells in Peripheral Blood Increased in Patients with Immune-Related Pancytopenia by Qing Shao, Yangyang Wang, Zhaoyun Liu, Hui Liu, Yihao Wang, Yang Zhao, Lijuan Li, Rong Fu

“…CD4+ lymphocytes were sorted by magnetic beads, and the mRNA expression levels of Th9 cells related transcription factors PU.1 and BATF were detected by RT-PCR. …”
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Efficient Therapeutic Function and Mechanisms of Human Polyclonal CD8+CD103+Foxp3+ Regulatory T Cells on Collagen-Induced Arthritis in Mice by Juan Sun, Yiming Yang, Xiaona Huo, Beibei Zhu, Zhenhua Li, Xueyu Jiang, Rufeng Xie, Li Gao, Ying Sun, Huahua Fan, Yongming Zhu, Jie Yang

“…Human CD8+T cells were isolated from human peripheral blood mononuclear cells and induced/expanded with TGF-β1 and RAPA along with anti-CD3/28 beads and IL-2 in vitro and harvested as hCD8+Tregs. …”
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The Intracellular Growth of M. tuberculosis Is More Associated with High Glucose Levels Than with Impaired Responses of Monocytes from T2D Patients by Martha Torres, María Teresa Herrera, Guadalupe Fabián-San-Miguel, Yolanda Gonzalez

“…To evaluate whether M. tuberculosis infection susceptibility is associated with an intrinsic factor in monocytes from type 2 diabetes (T2D) patients or it is associated with hyperglycemia per se, we analyzed TLR-2 and TLR-4 expression by flow cytometry and the cytokines IL-1β, IL-6, IL-8, IL-10, and TNF-α by cytometric bead array assays, either stimulated with TLR-2 and TLR-4 ligands or infected with M. tuberculosis in the whole blood from T2D patients (n=43) and healthy subjects (n=26) or in CD14+ monocytes from healthy subjects cultured in high glucose (HG) (30 mM). …”
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Le réseau hydraulique à Pompéi (Italie) de l’époque des Samnites à Auguste (fin ive-fin ier s. av. J.-C.) by Federico Giletti

“…Here the archaeological data has made it possible to trace the presence, below the later domus, of buildings from the 2nd century BC, built behind the city walls and basing part of their southernmost rooms on the sequence of cisterns with multiple vaulted chambers. …”
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Le flirt à Rabat au Maroc : Engagements des corps et arrangements socio-spatiaux by Chadia Arab, Christophe Guibert

“…The cornices and beaches are also places where young couples can meet, away from prying eyes, as shown in the photographs that illustrate the article. The couples behave like “teams”, as Erving Goffman (1973) formulates it, operating jointly a strict control of their gestures, representations, body distances and finally of the glances coming from outside. …”
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HEALING LANDSCAPES: THE EFFECTS OF ITS PARAMETERS ON DIFFERENT GENDER (CASE STUDY: ERAM GARDEN & AZADI PARK OF SHIRAZ-IRAN) by Mina Razmara, Hajar Asadpour, Malihe Taghipour

“…Considering the Canter Place model, the parameters of landscape healing presented in the form of three dimensions: functional, perceptual-semantic, and physical-visual. Since men and women behave differently in dealing with their surroundings, this research evaluates how Persian gardens and urban parks effect (with two different structural patterns) among men and women. …”
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The use of different 17β-estradiol hormone doses and water temperatures to control cannibalism in catfish Clarias gariepinus seed by Hylda Khairah Putri, Muhammad Zairin Jr., Odang Carman, Iis Diatin

“…A high level of testosterone is considered to cause catfish seeds to behave aggressively and subsequently encourage cannibalism. …”
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La mise en œuvre d’un aqueduc : l’exemple de la Brévenne à Lyon by Catherine Coquidé, Antoine Valois

“…But this kind of analysis makes it possible to understand the marks left behind, breaks in different stages of the assembly or the manual practices of the builders. …”
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Hubungan Keteraturan Penggunaan Kortikosteroid Inhalasi dengan Tingkat Kontrol Asma Pasien Berdasarkan ACT di Poliklinik Paru RSUP Dr. M. Djamil Padang by Wulan Prisilla Prisilla, Irvan Medison, Selfi Renita Rusjdi

“…Insiden terbanyak asma berada pada kelompok usia lanjut (60,4%). Sebagian besar pasien tidak teratur menggunakan kortikosteroid inhalasi (63,5%) dan pasien dengan asma tidak terkontrol memiliki proporsi tertinggi (59,4%) . …”
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Persistent Gender and Racial Hierarchies: Marriage Migration and Mixedness in Morocco from the French Protectorate to the Present by Catherine Therrien, Catherine Phipps

“…Finally, we examine the emotional motivations behind choosing mixed marriages, often overlooked by studies of migration, arguing that mixed marriage in Morocco has always shown a “desire for elsewhereness” and offered a space of freedom, a way to embrace new possibilities and turn away from certain social norms. …”
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Efisiensi Penggunaan QRIS Dengan Merchant Presented Mode Dalam Transaksi Pembayaran Non-Tunai by Aryo Pinandito, Raihan Fikri Brilliansyach

“…Seba-nyak 99% merchant yang berada di dalam daftar pengguna QRIS termasuk ke dalam usaha mikro, kecil, dan mene-ngah. …”
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Gambaran Status Gizi Pasien Tuberkulosis Anak di RSUP Dr. M. Djamil Padang by Chika Aulia Husna, Finny Fitry Yani, Machdawaty Masri Masri

“…Djamil Padang periode 1 Januari 2013 – 31 Desember 2013 dengan menggunakan metode total sampling, sehingga diperoleh 187 sampel.Hasil penelitian yang didapatkan yaitu tuberkulosis pada anak mayoritas berada di poliklinik anak 64% dengan perbandingan 1,8 : 1. …”
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