Recent Progress in Nanomaterial-Based Fluorescence Assays for the Detection of Food-Borne Pathogens by Shiyu Song, Lu Han, Min Chen, Leiqing Pan, Kang Tu

Subjects: Get full text

Correction: Fluorescent reporter plasmids for single-cell and bulk-level composition assays in E. faecalis. by Kelsey M Hallinen, Keanu A Guardiola-Flores, Kevin B Wood

Get full text

Validation and application of a visual LAMP assay for Mpox diagnosis — Insights from clinical samples in Thailand by Wansadaj Jaroenram, Yada Ongchanchai, Ravee Nitiyanontakij, Pawita Suwanwatthana, Ratchana Ponang, Wansika Kiatpathomchai

“…Despite numerous recent advances in infectious disease diagnostic technologies including quantitative (q) polymerase chain reaction (PCR) and antigen test kit (ATK), there remains a need to develop more efficient point-of-care assays for large-scale operations. In an effort towards such a screening test for low-resource settings, we have established a colorimetric loop-mediated isothermal amplification assay and detected the amplified DNA via naked-eye observation to maximize assay simplicity using monkeypox (Mpox) as a model. …”
Get full text

Novel (d)PCR assays for influenza A(H5Nx) viruses clade 2.3.4.4b surveillance by Antonio Marchini

“…RESULTS The JRC-MP assay detects influenza A viruses of different subtypes and origins, while the JRC-HA assay specifically detects HPAI A(H5Nx) 2.3.4.4b strains. …”
Get full text

Mercaptophenylboronic Acid‐Mediated Nanozyme Immunochromatographic Assay for Simultaneous Detection of Respiratory Bacteria and Virus by Qing Yu, Jiaxuan Li, Shuai Zheng, Yajin Hu, Benshun Tian, Meirou Lu, Bing Gu, Chongwen Wang

Subjects: Get full text

Turning up the heat: optimizing Bethesda assays for efanesoctocog alfa monitoring by Agathe Herb, Jordan Wimmer, Dominique Desprez, Olivier Feugeas, Laurent Mauvieux, Laurent Sattler

Get full text

Methodology for Wetland Seedbank Assays by Carrie Reinhardt Adams, Nancy M. Steigerwalt

“…ENH1090/EP354: Methodology for Wetland Seedbank Assays (ufl.edu) …”
Get full text

Validation of a liquid biopsy assay with increased sensitivity for clinical comprehensive genomic profiling by Xavier S. Bower, Jan Wignall, Matthew G. Varga, Joyce Zhu, Michael O'Sullivan, Naomi E. Searle, Lenny K. Hong, Turgut Dogruluk, Zeqian Li, Tiffany E. Farmer, Emilio Rosas-Linhard, Jason Luong, Esther Lin, Marie Erica Simon, David S. Tsao, John R. ten Bosch, Gary Palmer, Ajeet Gajra, Chanh Huynh, Wen Zhou

“…This study included analytical and clinical validation studies of Northstar Select, a plasma-based, tumor-naive CGP assay covering 84 genes. The assay detects SNV/Indels, CNVs (gain and loss), fusions, and microsatellite instability (MSI-H). …”
Get full text

Development and validation of a multiplex PCR assay with melt curve analysis for detecting simian Plasmodium in wild Macaca fascicularis by Thitiluck Swangsri, Rucksak Rucksaken, Wanat Sricharern, Wang Nguitragool, Naowarat Saralamba

“…We developed and validated a SYBR Green-based real-time PCR assay targeting the msp1 gene to detect and differentiate P. knowlesi, P. cynomolgi, and P. inui. …”
Get full text

Development and validation of the Normalized Organoid Growth Rate (NOGR) metric in brightfield imaging-based assays by Christophe Deben, Edgar Cardenas De La Hoz, Felicia Rodrigues Fortes, Maxim Le Compte, Sofie Seghers, Steve Vanlanduit, Hendrik Vercammen, Bert Van Den Bogert, Nelson Dusetti, Abraham Lin, Geert Roeyen, Marc Peeters, Hans Prenen, Filip Lardon, Evelien Smits

“…Here, we introduce the Normalized Organoid Growth Rate (NOGR) metric, specifically developed for brightfield imaging-based assays. A label-free image analysis model was applied to segment organoids precisely, track their growth rates over time, and classify viable and dead organoids. …”
Get full text

Simvastatin inhibits 20-Hydroxyeicosatetraenoic acid formation: docking and in vitro assay by Yazun Jarrar, Mansour Almansour, Mansour Al-Sayed Ahmad, Belal O. Al-Najjar, Amin A. Al-Doaiss, Sireen Abdul Rahim Shilbayeh, Su-Jun Lee

“…The binding affinity of simvastatin was evaluated based on lowest energy of binding (LEB) values, and interactions with the heme group were analyzed. Results In vitro assay showed that simvastatin, but not other tested statins, inhibited the formation of 20-HETE in the human liver microsome with IC50 value of 10 µM and in a competitive mechanism as shown in the kinetics of the Lineweaver-Burk plot. …”
Get full text

CoreTIA: a modular, statistically robust transduction inhibition assay for AAV neutralization by Beatrix Kovács, Beatrix Kovács, Beatrix Kovács, Fanni Somogyi, Fanni Somogyi, Viktória Szabó, Zoltán Zsolt Nagy, István Hernádi, Ferenc Mátyás, Wim Vanduffel, Zsuzsanna Szemlaky, Balázs Rózsa, Balázs Rózsa, István Ulbert, István Ulbert, István Ulbert, István Ulbert, Dániel Hillier, Dániel Hillier, Dániel Hillier

“…Evaluation and refinement of key assay parameters support consistent performance across AAV serotypes. …”
Get full text

Division of the large and multifunctional glycoside hydrolase family 2: high functional specificity and biochemical assays in the uncharacterized subfamilies by Annie Lebreton, Marie-Line Garron, Marlene Vuillemin, Bo Pilgaard, Bastian V. H. Hornung, Elodie Drula, Vincent Lombard, William Helbert, Bernard Henrissat, Nicolas Terrapon

Get full text

The Development of a Selective Colorimetric Sensor for Cu²⁺ and Zn²⁺ in Mineral Supplement with Application of a Smartphone Paper-Based Assay of Cu²⁺ in Water Samples by Mahmoud El-Maghrabey, Shōta Seino, Naoya Kishikawa, Naotaka Kuroda

Subjects: Get full text

Design and fabrication of smartphone based single paper lateral flow assay for early detection of PCOS biomarker by Aniket Nandi, Yash Kumar Gaur, Divyanshi Thakur, Ekta Daweshar, Kalicharan Sharma

Subjects: Get full text

Lupus anticoagulant associated with low grade B-cell lymphoma and IgM paraproteinaemia with lupus cofactor phenomenon on DRVVT and SCT assays - a possible novel association by Ting Hon Stanford Li, Pui Lun Yip, Hui Yiu Chan, Ho Kin Stephen Chan, Wai Shan Wong

“…The DRVVT assay in the first patient and SCT assay in second patient were falsely negative in the neat sample or less diluted sample, and the lupus anticoagulant activities were only revealed on dilution of samples with normal pooled plasma. …”
Get full text

Detection of hypervirulent Klebsiella pneumoniae (hvKp) strains directly from spiked blood cultures using a commercial Loop-Mediated isothermal amplification (LAMP) assay by Domingo Fernández Vecilla, Jorge Rodríguez Grande, Nuria Fraile Valcárcel, Zaira Moure García, Sergio García Fernández, María Siller Ruiz, María Pía Roiz Mesones, José Luis Díaz de Tuesta del Arco, Mikel Joseba Urrutikoetxea Gutiérrez, María Catalina Lomoro, María Carmen Fariñas, Alain Ocampo-Sosa

“…The Eazyplex® hvKp LAMP assay was tested on spiked blood cultures inoculated with hvKp isolates. …”
Get full text

Exploring and evaluating the in vitro antifungal, antibiofilm and antitoxin efficacy of Artabotrys brachypetalus Benth. against Candida spp. and its toxicity in vivo assays by Camila de Paula Siqueira, Ralciane de Paula Menezes, Meliza Arantes Souza Bessa, Nágela Bernadelli Sousa Silva, Domingos Augusto João, Tiara da Costa Silva, Diego Godina Prado, Sérgio Antônio Lemos de Morais, Alberto de Oliveira, Francisco José Torres de Aquino, Luís Carlos Scalon Cunha, Carlos Henrique Gomes Martins

“…For the expression of introducer exoenzymes (½MIC), the ethanolic root extract showed a significant hemolytic activity of 42.7% against C. albicans; in assays on C. elegans, it demonstrated toxicity above a concentration of 1,000 µg/mL. …”
Get full text

One-hour extraction-free loop-mediated isothermal amplification HPV DNA assay for point-of-care testing in Maputo, Mozambique by Maria J. Barra, Alexis F. Wilkinson, Ariel E. Ma, Karthik Goli, Hira Atif, Nafissa M.R.B. Osman, Cesaltina Lorenzoni, Guilhermina Tivir, Eva H. Lathrop, Philip E. Castle, Ming Guo, Jane R. Montealegre, Ellen S. Baker, Mila P. Salcedo, Kathleen M. Schmeler, Rebecca R. Richards-Kortum

“…We analytically evaluate our assay with clinical samples in Houston, Texas (n = 38) and Maputo, Mozambique (n = 191). …”
Get full text

A high-resolution, nanopore-based artificial intelligence assay for DNA replication stress in human cancer cells by Mathew J. K. Jones, Subash Kumar Rai, Pauline L. Pfuderer, Alexis Bonfim-Melo, Julia K. Pagan, Paul R. Clarke, Francis Isidore Garcia Totañes, Catherine J. Merrick, Sarah E. McClelland, Michael A. Boemo

“…Abstract DNA replication stress is a hallmark of cancer that is exploited by chemotherapies. Current assays for replication stress have low throughput and poor resolution whilst being unable to map the movement of replication forks genome-wide. …”
Get full text