Evolutionary and functional divergence of Sfx, a plasmid-encoded H-NS homolog, underlies the regulation of IncX plasmid conjugation by Avril Wang, Martha Cordova, William Wiley Navarre

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Bacterial interactions underpin worsening lung function in cystic fibrosis-associated infections by Damian W. Rivett, Lauren R. Hatfield, Helen Gavillet, Michelle Hardman, Christopher van der Gast

“…The statistical ecology approach presented here enables clear hypotheses from observational data that can be ratified through subsequent manipulative experimental studies. Moreover, it can also be used to support the design and construction of clinically relevant in vitro models of polymicrobial infections.IMPORTANCEResearch studies have repeatedly demonstrated that chronic lung infection in cystic fibrosis is polymicrobial and consequently does not adhere to the single microbe-based Koch’s postulates. …”
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Multi-omics mechanical analysis of gut microbiota, carboxylic acids, and cardiac gene expression interaction triggering diabetic cardiomyopathy by Meixin Shi, Bingbing Zhao, Wenjie Cai, Hui Yuan, Xiao Liang, Zhitao Li, Xinyu Liu, Ye Jin, Xi Liu, Can Wei

“…According to metagenomics, the gut microbiota of mice in the db/db group were disrupted, and particularly Lachnospiraceae bacteria and Oscillospiraceae bacteria significantly decreased. Also, according to the Pearson correlation analysis, a significant positive correlation was found between CAs and PPAR signaling pathway-related DEGs, and a negative correlation was found between CAs and the abundance of the above-mentioned species. …”
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Overexpressing the ClpC AAA+ unfoldase accelerates developmental cycle progression in Chlamydia trachomatis by Aaron A. Jensen, Saba Firdous, Lei Lei, Derek J. Fisher, Scot P. Ouellette

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MultiTax-human: an extensive and high-resolution human-related full-length 16S rRNA reference database and taxonomy by Zhiwei Bao, Bin Zhang, Jianhua Yao, Ming D. Li

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RETRACTED: Melanin Produced by Bordetella parapertussis Confers a Survival Advantage to the Bacterium during Host Infection by Yukihiro Hiramatsu, Takashi Nishida, Dendi Krisna Nugraha, Fuminori Sugihara, Yasuhiko Horiguchi

“…Mouse infection experiments also showed that the hppD-deficient mutant was eliminated from the respiratory tract more rapidly than the wild-type strain, although the initial colonization levels were comparable between the two strains. …”
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Naturally occurring influenza reassortment in pigs facilitates the emergence of intrahost virus subpopulations with distinct genotypes and replicative fitness by Chong Li, Victoria Meliopoulos, Aaron Rendahl, Stacey Schultz-Cherry, Montserrat Torremorell

“…Understanding the extent of IAV reassortment occurring at the individual pig level in naturally infected pigs and how reassortment impacts virus diversity, persistence, and replicative fitness is essential for countering IAV threats to humans and animals. Here, 244 IAV plaques were isolated from 24 commercial pigs, resulting in 26 distinct genome segment constellations. …”
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The Mla pathway promotes Vibrio cholerae re-expansion from stationary phase by Deborah R. Leitner, Franz G. Zingl, Alexander A. Morano, Hailong Zhang, Matthew K. Waldor

“…The mutant defect in transitioning out of stationary phase into active growth (culturability) was also observed in monocultures at 48 h. However, by 96 h the culturability of the WT and mutant strains were equivalent. …”
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Antimicrobial resistance, virulence gene profiles, and molecular epidemiology of enterococcal isolates from patients with urinary tract infections in Shanghai, China by Weiyi Wu, Shuzhen Xiao, Lizhong Han, Qiong Wu

“…ABSTRACT Urinary tract infections (UTIs) are among the most prevalent infectious diseases, yet there is still limited understanding of the epidemiology of Enterococcal strains isolated from UTI patients in Shanghai. This study aims to elucidate the antimicrobial resistance profiles, virulence gene carriage, and molecular epidemiology of selected Enterococcal strains from UTI patients in Shanghai. …”
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A quantitative PCR to detect non-toxigenic Clostridioides difficile by Khurshida Begum, Hubert C. Chua, M. Jahangir Alam, Kevin W. Garey, Jinhee Jo

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Histological staining of tick bite skin biopsies for spirochetes and Powassan virus RNA by Jihane Oufattole, Anne Piantadosi, Sam R. Telford, Alvaro C. Laga, Isaac H. Solomon

“…To validate these assays, we also tested the same stains on histological sections from whole ticks infected with spirochetes or Powassan virus. …”
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Transendothelial migration of the Lyme disease spirochete involves spirochete internalization as an intermediate step through a transcellular pathway that involves Cdc42 and Rac1 by Daiana Alvarez-Olmedo, Claire Kamaliddin, Theodore B. Verhey, May Ho, Rebekah De Vinney, George Chaconas

“…Our new experimental system also provides a new avenue for further studies of this important process.…”
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Age but not vitamin D is related to sarcopenia in vitamin D sufficient male elderly in rural China by Weinuo Mi, Huifeng Zhang, Lina Zhang, Xingjia Li, Zhiguo Wang, Yu Sun, Tonggao Shen, Kuanlu Fan, Chao Liu, Shuhang Xu

“…Abstract This study aimed to identify the correlation of serum 25(OH)D level with sarcopenia and its components in Chinese elderly aged 65 years and above from rural areas. …”
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Association between body composition indices and vascular health: a systematic review and meta-analysis by Mohammad Nikoohemmat, Amirhossein Ramezani Ahmadi, Ali Valizadeh, Seyed Mohammadmisagh Moteshakereh, Reza Yari-Boroujeni, Zahra Seifi, Majid Valizadeh, Behnaz Abiri

“…Subgroup analyses revealed a non-significant association between appendicular skeletal muscle (ASM) and IMT (ES: 1.01, 95% CI 0.76–1.35). Conclusion This meta-analysis highlights the complex relationship between body composition and vascular health. …”
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Genome evolution following an ecological shift in nectar-dwelling Acinetobacter by Vivianna A. Sanchez, Tanya Renner, Lydia J. Baker, Tory A. Hendry

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Reverse transcriptase inhibitors diminish systemic proinflammatory responses to bacterial pathogens by Karthik Hullahalli, Katherine G. Dailey, Ryan Acbay, Masataka Suzuki, George I. Balazs, Matthew K. Waldor

“…Since liver necrosis is linked to heightened systemic inflammatory responses, we hypothesized that NRTIs diminish inflammation caused by E. coli infection and may also have broad impacts on the systemic immune response to bacterial pathogens. …”
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The multidisciplinary approach to the treatment of paediatric chronic pain: a qualitative analysis through focus groups by Aldana Paola Ercolano, Sabrina Galvan, Tessa Louise Marchesan, Alessia Gavioli, Gerardo Papa, Mariarita Todaro, Lidia Berforini D’Aquino, Alessia Zanatta, Andrea Vianello

“…The importance of preventing physical complications and improving the child’s quality of life also emerged. Discussion: The results confirm the importance of a holistic, multidisciplinary approach to managing pediatric chronic pain. …”
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Protocol for the DisCoVeRy trial: multicentre, adaptive, randomised trial of the safety and efficacy of treatments for COVID-19 in hospitalised adults by Florence Ader, Jean-François Timsit, Yazdan Yazdanpanah, François-Xavier Lescure, Laetitia Moinot, Christelle Delmas, Hélène Espérou, Laurent Abel, Benjamin Leveau, Vinca Icard, Toni Alfaiate, Linda Wittkop, Charles Burdet, France Mentré, Marion Schneider, Nathan Peiffer-Smadja, Julien Poissy, Lila Bouadma, Bruno Lina, Florence Morfin-Sherpa, Maude Bouscambert, Alexandre Gaymard, Gilles Peytavin, Jeremie Guedj, Claire Andrejak, Cedric Laouenan, Drifa Belhadi, Axelle Dupont, Basma Basli, Anissa Chair, Samira Laribi, Julie Level, Marie-Capucine Tellier, Aline Dechanet, Dominique Costagliola, Sandrine Couffin-Cadiergues, Juliette Saillard, Claire Fougerou, Carole Cagnot, Soizic Le Mestre, Delphine Lebrasseur-Longuet, Ventzislava Petrov-Sanchez, Alpha Diallo, NoéMie Mercier, Sarah Tubiana, Benjamin Hamze, Ambre Gelley, Marion Noret, Eric D’Ortenzio, Oriane Puechal, Caroline Semaille

“…A sample size of 3100 patients (620 patients per arm) is targeted. This trial has begun on 22 March 2020. …”
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Two rice cultivars recruit different rhizospheric bacteria to promote aboveground regrowth after mechanical defoliation by Changjin Jiang, Fei Wang, Jinling Tian, Wanyuan Zhang, Kabin Xie

“…The microbiome analysis also led us to find a Uliginosibacterium gangwonense strain that promoted rice cv. …”
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Whole-genome phylogenetic analysis of Mycobacterium avium complex from clinical respiratory samples by Chew Ka Lip, Joelle Go, Nur Aisyah Binte Abu Bakar, Sophie Octavia, Raymond Tzer Pin Lin, Jeanette W. P. Teo

“…ABSTRACT The Mycobacterium avium complex (MAC) is a common causative agent causing nontuberculous mycobacterial (NTM) pulmonary disease worldwide. …”
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