Virulence factor discovery identifies associations between the Fic gene family and Fap2+ fusobacteria in colorectal cancer microbiomes by Geicho Nakatsu, Duhyun Ko, Monia Michaud, Eric A. Franzosa, Xochitl C. Morgan, Curtis Huttenhower, Wendy S. Garrett

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Gut dysbiosis narrative in psoriasis: matched-pair approach identifies only subtle shifts correlated with elevated fecal calprotectin by Bayazit Yunusbayev, Anna Bogdanova, Nadezhda Nadyrchenko, Lavrentii Danilov, Viktor Bogdanov, Grigory Sergeev, Radick Altinbaev, Fanil Bilalov, Milyausha Yunusbaeva

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Development of SacB-based counterselection for efficient allelic exchange in Fusobacterium nucleatum by Peng Zhou, Bibek G C, Bo Hu, Chenggang Wu

“…Previously, the galk-based allelic exchange method, although effective, faced an inherent limitation—the need for a modified host. This study aims to surmount this limitation by substituting galK with sacB for gene modification in F. nucleatum. …”
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The Campylobacter jejuni BumS sensor phosphatase detects the branched short-chain fatty acids isobutyrate and isovalerate as direct cues for signal transduction by Nestor Ruiz, Jiawei Xing, Igor B. Zhulin, Chad A. Brautigam, David R. Hendrixson

“…Here, we expanded the repertoire of cues sensed by BumS to the branched-short chain fatty acids isobutyrate and isovalerate that are also abundant in the lower intestines. Both isobutyrate and isovalerate are potent, direct cues for BumS, whereas butyrate is an indirect cue. …”
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Improvement of a MALDI-TOF database for the reliable identification of Candidozyma auris (formally Candida auris) and related species by Hui-Hui Zhu, Miao-Miao Liu, Teun Boekhout, Qi-Ming Wang

“…This method will play a crucial role in accurately diagnosing infectious agents of the genus Candidozyma in clinical practice.IMPORTANCEImportance Candidozyma auris, also known as Candida auris, has quickly spread across the world, and prompt identification of C. auris from infected individuals is critical. …”
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Advancing dental biofilm models: the integral role of pH in predicting S. mutans colonization by Jay S. Sangha, Valentina Gogulancea, Thomas P. Curtis, Nicholas S. Jakubovics, Paul Barrett, Aline Metris, Irina D. Ofiţeru

“…We have simulated all the conditions studied experimentally, considering different initial relative abundance of the five species, and also different initial clustering in the biofilm. …”
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Progression of ampC amplification during de novo amoxicillin resistance development in E. coli by Luyuan Nong, Martijs Jonker, Wim de Leeuw, Meike T. Wortel, Benno ter Kuile

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CDK12 antagonizes a viral suppressor of RNAi to modulate antiviral RNAi in Drosophila by Liqin Zhang, Yu Liang, Jiayu Qin, Chen Liu, Mengwei Shang, Xiaoming Sun

“…Furthermore, the knockdown of CDK12 inhibited the production of vsiRNAs in FHV-infected Drosophila cells. This study revealed that CDK12 mediated a counter-counter-defense strategy against VSR, thereby enhancing antiviral RNAi immunity in Drosophila.IMPORTANCEThe arms race between virus and host immunity is never-ending. …”
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Palmitoleic acid sensitizes vancomycin-resistant Staphylococcus aureus to vancomycin by outpacing the expression of resistance genes by Zajeba Tabashsum, Michelle Angeles-Solano, Ashelyn E. Sidders, Joshua B. Parsons, Sarah E. Rowe

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A bacterial membrane-disrupting protein stimulates animal metamorphosis by Kyle E. Malter, Tiffany L. Dunbar, Carl Westin, Emily Darin, Josefa Rivera Alfaro, Nicholas J. Shikuma

“…ABSTRACT Diverse marine animals undergo a metamorphic larval-to-juvenile transition in response to surface-bound bacteria. …”
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Development of ptxD/Phi as a new dominant selection system for genetic manipulation in Cryptococcus neoformans by Muthita Khongthongdam, Tanaporn Phetruen, Sittinan Chanarat

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The probability of detecting host-specific microbial source tracking markers in surface waters was strongly associated with method and season by Claire M. Murphy, Daniel L. Weller, Tanzy M. T. Love, Michelle D. Danyluk, Laura K. Strawn

“…While many methods for understanding fecal contamination cannot differentiate between human and animal sources, microbial source tracking (MST) marker methods are used to identify fecal sources. …”
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Association of intratumoral microbiome diversity with hepatocellular carcinoma prognosis by Fengle Jiang, Yuan Dang, Zheting Zhang, Yanan Yan, Yingchao Wang, Yi Chen, Lihong Chen, Jialiang Zhang, Jingfeng Liu, Jianmin Wang

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Mono-allelic epigenetic regulation of polycistronic transcription initiation by RNA polymerase II in Trypanosoma brucei by Rudo Kieft, Laura Cliffe, Haidong Yan, Robert J. Schmitz, Stephen L. Hajduk, Robert Sabatini

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Trends in Mycoplasma pneumoniae infections in pediatric patient preceding, during, and following the COVID-19 pandemic: a comprehensive longitudinal analysis by Jianwei Zhang, Ruoya Wu, Liyan Mo, Jinlong Ding, Ke Huang

“…ABSTRACT This study investigated the impact of the coronavirus disease 2019 (COVID-19) pandemic and its associated restrictive measures on Mycoplasma pneumoniae infections in children with acute respiratory tract infection. The study aimed to elucidate the epidemiological characteristics of M. pneumoniae infections before and during the pandemic and following the easing of restrictive measures. …”
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Combined mutations in nonstructural protein 14, envelope, and membrane proteins mitigate the neuropathogenicity of SARS-CoV-2 Omicron BA.1 in K18-hACE2 mice by Kotou Sangare, Shufeng Liu, Prabhuanand Selvaraj, Charles B. Stauft, Matthew F. Starost, Tony T. Wang

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DDO-adjuvanted influenza A virus nucleoprotein mRNA vaccine induces robust humoral and cellular type 1 immune responses and protects mice from challenge by Victoria Gnazzo, Hanaa Saleh, Ítalo A. Castro, Adrianus C. M. Boon, Amelia K. Pinto, James D. Brien, Carolina B. López

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The role of morphological adaptability in Vibrio cholerae’s motility by Jun Xu, Keigo Abe, Toshio Kodama, Marzia Sultana, Denise Chac, Susan M. Markiewicz, Hideyuki Matsunami, Erika Kuba, Shiyu Tsunoda, Munirul Alam, Ana A. Weil, Shuichi Nakamura, Tetsu Yamashiro

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The Vibrio type VI secretion system induces intestinal macrophage redistribution and enhanced intestinal motility by Julia S. Ngo, Piyush Amitabh, Jonah G. Sokoloff, Calvin Trinh, Travis J. Wiles, Karen Guillemin, Raghuveer Parthasarathy

“…Inoculation with Vibrio gives rise to strong, ACD-dependent, gut contractions whose magnitude resembles those resulting from genetic depletion of macrophages. Vibrio also induces tissue damage and macrophage activation, both ACD-dependent, recruiting macrophages to the site of tissue damage and away from their unperturbed positions near enteric neurons that line the midgut and regulate intestinal motility. …”
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Development of a metabolome-based respiratory infection prognostic during COVID-19 arrival by John I. Robinson, Laura R. Marks, Andrew L. Hinton, Jane A. O'Halloran, Charles W. Goss, Peter J. Mucha, Jeffrey P. Henderson

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