An amplicon-based approach for full-genome characterization of HPV16 by Isabelle Malet, Inès Draa, Valentin Leducq, Fanny Vuong, Pascale Bonnafous, Anne-Geneviève Marcelin, Vincent Calvez, Aude Jary

“…In addition, the specificity of the method was validated by the absence of amplification of other high-risk HPV genotypes compared with HPV16. …”
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Onboard Decision-making System of Unmanned Mine Truck by WANG Jia, HUANG Jiade, LIU Zhicong, HUANG Peng, LYU Liang

“…At present, the research on unmanned mine truck system on-board decision system in mine scenarios at home and abroad is still in the initial stage, and its research focus is mainly on global planning and scheduling to improve overall safety and efficiency of the system, while ignoring the research on the mine truck independent decision method based on the global planning and scheduling. …”
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Advancements in DNA methylation technologies and their application in cancer diagnosis by Yang Yang, Xiaosha Wen, Li Wang

“…In this review, we discuss various methods for detecting DNA methylation, focusing on bisulfite conversion-based techniques, methylation-sensitive restriction enzyme methods, enzyme conversion-based methods, third-generation sequencing approaches, and artificial intelligence. …”
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Investigation of DC-Biased Optical OFDM With Precoding Matrix for Visible Light Communications: Theory, Simulations, and Experiments by Tao Jiang, Ming Tang, Rui Lin, Zhenhua Feng, Xi Chen, Lei Deng, Songnian Fu, Xiang Li, Wu Liu, Deming Liu

“…To figure out the intrinsic laws of a PM method, we investigate the principles of PAPR reduction, clipping distortion optimization, and signal-to-noise ratio (SNR) distribution equalization. …”
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Constructing the Mandarin Phonological Network: Novel Syllable Inventory Used to Identify Schematic Segmentation by Karl D. Neergaard, Chu-Ren Huang

“…In both tasks, participants were found to manipulate lexical items at each possible syllable location, but preferring to maintain whole syllables while manipulating lexical tone in their search through the mental lexicon. …”
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Reflections and implementation strategies for constructing a standard system for deep coalbed methane exploration and exploitation by Xinjun CHEN, Zengqin LIU, Baojian SHEN, Shihu ZHAO, Jiaqi ZHANG, Jincheng YE

“…The medium-term objective is to establish a technical standard cluster covering the entire industry chain while promoting the whole life cycle risk management, with the purpose of achieving efficient resource utilization and maximizing investment returns. …”
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Phenotypic and Genotypic Characterization of ESBL-, AmpC-, and Carbapenemase-Producing <i>Klebsiella pneumoniae</i> and High-Risk <i>Escherichia coli</i> CC131, with the First Repo... by Hajer Ziadi, Fadela Chougrani, Abderrahim Cheriguene, Leticia Carballeira, Vanesa García, Azucena Mora

“…Whole genome sequencing (WGS) was performed on the ST1193 clone. …”
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Cost-effective promoter methylation analysis via target long-read bisulfite sequencing: a case study in severe preterm birth by Silvana Pereyra, Angela Sardina, Rita Neumann, Celia May, Rossana Sapiro, Bernardo Bertoni, Mónica Cappetta

“…In contrast, targeted sequencing provides an optimal, cost-effective solution when focusing on specific candidate regions while providing sufficient sequencing depth. …”
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Application of the Bionic Concept in Reducing the Complexity Noise and Drag of the Mega High-Speed Train Based on Computer Simulation Technologies by He-xuan Hu, Bo Tang, Ye Zhang

“…Regarding the continuous development of high-speed trains and the increase of running speeds, the aerodynamic design of high-speed trains has become significantly important, while reduction of drag and noise comprises a significant challenge in order to optimize aerodynamic design of high-speed trains. …”
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Fluorescent reporter plasmids for single-cell and bulk-level composition assays in E. faecalis. by Kelsey M Hallinen, Keanu A Guardiola-Flores, Kevin B Wood

“…This approach offers a simple and scalable method for selection and competition experiments in simple two-species populations under aerobic growth conditions. …”
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Digital Mapping of Soil Drainage Classes Using Multitemporal RADARSAT-1 and ASTER Images and Soil Survey Data by Mohamed Abou Niang, Michel Nolin, Monique Bernier, Isabelle Perron

“…The classification models were then applied to these subsets for each land use and merged to obtain a digital soil drainage map for the whole watershed. The DTC method provided better classification accuracies (29 to 92%) than the DAC method (33 to 79%) according to the land use type. …”
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Neuromorphic Digital-Twin-Based Controller for Indoor Multi-UAV Systems Deployment by Reza Ahmadvand, Sarah Safura Sharif, Yaser Mike Banad

“…Our framework addresses critical limitations of conventional methods, including the dependence on premodeled environments, computational intensity of traditional methods, and local minima issues in potential field approaches. …”
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Describing dietary patterns of high-intensity functional training athletes: a cross-sectional study by Linh Nguyen, Kworweinski Lafontant, Claudia Gonzalez, Susan Kampiyil, Michelle Da Silva Barbera, Jack Livingston, Sofea Smith, Jeffrey R. Stout, David H. Fukuda

“…Most HIFT athletes consume at least two daily servings of fruits, whole-grains, and dairy while avoiding ultra-processed foods. …”
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An immunoinformatics approach in designing high-coverage mRNA multi-epitope vaccine against multivariant SARS-CoV-2 by Ernawati Arifin Giri-Rachman, Al Mirahma Febri Kurnianti, Rizarullah, Aditya Hanung Setyadi, Anita Artarini, Marselina Irasonia Tan, Catur Riani, Dessy Natalia, Reza Aditama, Husna Nugrahapraja

“…In this study, utilizing immunoinformatics and reverse vaccinology, we aimed to develop a multi-epitope mRNA vaccine with high population coverage, targeting multiple variants of SARS-CoV-2. Methods: To design a multivariant vaccine, 20,567 sequences consisting of all SARS-CoV-2′s variants of concern whole genome were retrieved. …”
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Atrial fibrillation reduces CSF flow dynamics. A multimodal MRI study by Sabine Hofer, Marlena Schnieder, Leonie Polster, Peter Dechent, Mathias Bähr

“…T1-weighted imaging segmented brain tissue and ventricular size, while fast T1Flash examined tissue anatomy. EPI diffusion assessed fluid motion along the perivascular space, and artefact-free STEAM diffusion described whole-brain CSF dynamics. …”
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Evolution of strain diversity and virulence factor repertoire in pediatric Staphylococcus aureus isolates. by Margaret Free, Nicole Soper, James C Slaughter, Andries Feder, Colleen Bianco, Ahmed M Moustafa, Paul Planet, C Buddy Creech, Isaac Thomsen

“…With rising antimicrobial resistance, optimal prevention strategies and novel therapeutics are needed. …”
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Crystal growth behavior of nanocrystal anatase TiO2: A Rietveld refinement in WPPF analysis by Md. Tarikul Islam, Sahil Aman, Taiful Bayzid, Md. Ashikur Rahaman, Upoma Podder, Gazi. Md. Arifuzzaman Khan, Md. Ashraful Alam

“…Novel crystallographic investigation of sol-gel-derived anatase TiO2 nanocrystals synthesized using tetra isopropyl orthotitanate (TIOT) as a precursor at pH 9.0 for hydrolysis promoter. Unlike conventional methods integrated whole powder pattern fitting (WPPF) based Rietveld refinement to quantitatively analyze the crystal structure, phase purity and microstructural features of the synthesized nanoparticles (NPs). …”
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Lytic bacteriophages targeting multidrug-resistant Pseudomonas aeruginosa in Moschus berezovskii: isolation, characterization, and therapeutic efficacy against bacteremia by Qingxia Hu, Yanjie Zhang, Ni Lv, Yahao Kang, Saba Nasir, Ruiqing Wang, Jiahao Qu, Jiadeng Jiang, Xiao Li, Xinglong Wang

“…The safety and practicality of the two phages were analyzed through methods such as biological characteristic assessment, whole genome sequencing analysis, and animal experiments. …”
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Vineyard pruning-wood waste valorisation: sustainable extraction of bioactive compounds by Elisabetta Tumminelli, Valeria Cavalloro, Valeria Cavalloro, Chiara Ingrà, Alessandra Ferrandino, Alessio Porta, Giorgio Marrubini, Emanuela Martino, Emanuela Martino, Daniela Rossi, Simona Collina

“…Keeping in mind eco-sustainable criteria, the greenness of the UHPLC method was evaluated through the open source calculator AGREE: analytical GREennEss Calculator 0.5 beta, while the environmental impact of the whole procedure proposed for the extraction and the isolation of the secondary metabolites was determined using the environmental impact factor (EF), obtaining satisfactory results.…”
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Rapid development of homozygous lines through culture of isolated microspores in leafy crops of <i>Brassicaceae</i> Burnett by Elena A. Domblides, Olga A. Chichvarina, Anna I. Minejkina, Evgeniу L. Kurbakov, Viktor A. Kharchenko, Arthur S. Domblides, Alexey V. Soldatenko

“…RelevanceBiotechnological methods are generally used to speed up breeding programs and to enhance genetic diversity, so the culture of isolated microspore in vitro can be regarded as one of very suitable methods. …”
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