Bacterial gastroenteritis in the world of culture-independent diagnostic testing: a study to evaluate the kinetics of bacterial shedding by culture and CIDT by Nathan K. McCartney, Surangi H. Thilakarathna, Tara Hluchy, Dylan R. Pillai, Linda Chui, Byron M. Berenger

“…Stool samples were submitted at weeks 2, 3, 4, 5, and 6 post-diagnosis. Stools were tested using the commercial nucleic acid-based BD MAX Enteric Bacterial Panel (CIDT method) and routine culture methods. …”
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Gliogenesis from the subventricular zone modulates the extracellular matrix at the glial scar after brain ischemia by Maria Ardaya, Marie-Catherine Tiveron, Harold Cremer, Esther Rubio-López, Abraham Martín, Benjamin Dehay, Fernando Pérez-Cerdá, Carlos Matute, Federico N Soria, Fabio Cavaliere

“…However, the activation of gliogenesis and the role of newly formed astrocytes in the post-ischemic scenario remain subjects of debate. …”
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The β-triketone, nitisinone, kills insecticide-resistant mosquitoes through cuticular uptake by Zachary Thomas Stavrou-Dowd, George Parsons, Clair Rose, Faye Brown, Rosemary Susan Lees, Álvaro Acosta-Serrano, Lee Rafuse Haines

“…Mortality was recorded at 30 min and 24, 48, and 72 h post-exposure under controlled conditions. A total of 3 biological replicates of 30 mosquitoes per treatment were used. …”
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Unraveling molecular interconnections and identifying potential therapeutic targets of significance in obesity-cancer link by Alanoud Abdulla, Hana Q. Sadida, Jayakumar Jerobin, Imadeldin Elfaki, Rashid Mir, Sameer Mirza, Mayank Singh, Muzafar A. Macha, Shahab Uddin, Khalid Fakhro, Ajaz A. Bhat, Ammira S. Al-Shabeeb Akil

“…It also increases the risk of various cancers, including melanoma, endometrial, prostate, pancreatic, esophageal adenocarcinoma, colorectal carcinoma, renal adenocarcinoma, and pre-and post-menopausal breast cancer. Obesity-induced cellular changes, such as impaired CD8+ T cell function, dyslipidemia, hypercholesterolemia, insulin resistance, mild hyperglycemia, and fluctuating levels of leptin, resistin, adiponectin, and IL-6, contribute to cancer development by promoting inflammation and creating a tumor-promoting microenvironment rich in adipocytes. …”
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Effects of acute temperature exposure duration on plasma metabolites and gene expression levels in liver and brain of juvenile olive flounder (Paralichthys olivaceus) by Sulhye Park, Jeongwhui Hong, Haham Kim, Hyuncheol Jeon, Deni Aulia, Dain Lee, Seunghyung Lee

“…Following exposure, samples of whole blood, liv er, and brain were collected at 0, 2, 4, 6, 12, 24, and 48 h post-exposure, after recovery at 20°C. Plasma hematocrit, glutamate oxaloacetate transaminase (GOT), glutamate pyruvic transaminase (GPT), glucose, total protein, triglycerides, and total cholesterol levels were measured. …”
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Spatiotemporal Dynamics of Macrobenthic Communities and Environmental Factors in the Aquatic Vegetation Restoration Zone of Baimao Bay by Weiwei Wei, Ning Hu, Chunhua Li, Chun Ye, Kexin Miao, Yang Wang, Xian Xiao, Yuan Zhao, Youde Yang, Liangkui Lai

“…Multivariate analysis revealed a fundamental ecological driver shift where post-restoration pH and transparency replaced nutrients as dominant factors, reducing total nitrogen/total phosphorus influence by 40–60%, while filter-feeding species (predominantly bivalves and gastropods) became the dominant macrobenthic biomass group (72.4%) with pollution-tolerant oligochaetes decreasing by 69.1% in abundance, alongside distinct spatial heterogeneity showing pH-regulated lakeshore communities (8.37 to 8.45), transparency-governed shallow-water communities (<i>H</i>′ = 1.35), and a residual nutrient-influenced deep-water area, with a shallow-water area (<2.5 m) unexpectedly exhibiting 3.2 times higher biomass (222.51 g/m²) than deep waters, highlighting vegetation-mediated habitat optimization. …”
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Effectiveness of wellness program interventions to improve physician wellness: a systematic review by Nikola Kolobaric, Benjamin Milone, Maria Salman, Amos Buh, Nandini Biyani, Gamal Wafy, Stephen G Fung, Mekaylah Scott, Rohan Kiska, Rachel Kang, Kathryn Lee, Sarah Syed, Maya Gibb, Shan Dhaliwal, Daniel Myran, Courtney Maskerine, Pierre Antoine Brown, Ayub Akbari, Gregory L. Hundemer, Peter Tanuseputro, Deena Fremont, Edward Spilg, Manish M. Sood

“…The efficacy of interventions varied, with sixteen studies (44.4%) demonstrating some measurable degree of effectiveness, with statistically significant changes (p < 0.05) post-intervention. Few studies reported improvements by physician sex, age groups, or comparisons across specialities. …”
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Mechanistic divergence between SOS response activation and antibiotic-induced plasmid conjugation in Escherichia coli by Ruoxuan Zhao, Arkadiusz Nawrocki, Jakob Møller-Jensen, Gang Liu, John Elmerdahl Olsen, Line Elnif Thomsen

“…In contrast, the SOS hyper-induced strain displayed limited upregulation of plasmid-encoded proteins, suggesting post-transcriptional regulation. Additionally, putative LexA binding sites on the IncI1 plasmid revealed potential SOS-mediated regulation of plasmid genes, highlighting the interaction between the SOS response and plasmid, although it did not significantly affect conjugation.IMPORTANCEPlasmids play a critical role in the dissemination of antibiotic resistance through conjugation. …”
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Neonatal-Inspired Reprogramming of Microglial Pan-Programmed Cell Death Enhances Regeneration in Adult Spinal Cord Injury by Beibei Yu, Yongfeng Zhang, Yujie Yang, Shijie Yang, Haining Wu, Xue Gao, Yiming Hao, Shengyou Li, Bing Xia, Jintao Liu, Lingli Guo, Borui Xue, Mingze Qin, Huangtao Chen, Jianzhong Li, Shouping Gong, Teng Ma, Jinghui Huang

“…Here, we identified neonatal mice with balanced PCD post-SCI as an ideal model for adult SCI. Accordingly, we developed “Thanatoset”, an SCI-specific gene panel to map tissue and cellular pan-PCD dynamics across neonatal and adult mice. …”
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The use of drone-delivered Automated External Defibrillators in the emergency response for out-of-hospital cardiac arrest. A simulation study by Christopher M. Smith, Carl Powell, Celia J. Bernstein, Harry Howe, Mark Holt, Mary O’Sullivan, Keith Couper, Nigel Rees

“…We used video-recording, emergency-call audio and post-simulation interviews to gather additional information about participants’ experiences. …”
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Systematic Review and Meta-Analysis of the Y-Balance Test Lower Quarter: Reliability, Discriminant Validity, and Predictive Validity by Phillip Plisky, Katherine Schwartkopf-Phifer, Bethany Huebner, Mary Beth Garner, Garrett Bullock

“…# Background Deficits in dynamic neuromuscular control have been associated with post-injury sequelae and increased injury risk. The Y-Balance Test Lower Quarter (YBT-LQ) has emerged as a tool to identify these deficits…”
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Investigation of overlap effects in abrasive water jet strengthening of carburized 18CrNiMo7–6 alloy steel by Lan-rong Liu, Bao-cheng Sun, Guang-tao Xu, Yong-tao Ma, Ming-hao Zhao, Zhen-ye Zhao

“…Furthermore, the discrepancies between theoretical and experimental peak and trough values post-multipass overlap are found to be in good concordance. …”
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Detection of Cathelicidin-1 and Cathelicidin-2 Biomolecules in the Milk of Goats and Their Use as Biomarkers for the Diagnosis of Mastitis by Maria V. Bourganou, Dimitra V. Liagka, Konstantinos Vougas, Daphne T. Lianou, Natalia G. C. Vasileiou, Konstantina S. Dimoveli, Antonis P. Politis, Nikos G. Kordalis, Efthymia Petinaki, Vasia S. Mavrogianni, George Th. Tsangaris, George C. Fthenakis, Angeliki I. Katsafadou

“…In an experimental study, after inoculation of <i>Staphylococcus simulans</i> into a mammary gland of goats, bacteriological and cytological examinations of milk samples, as well as proteomics examinations [two-dimensional gel electrophoresis analysis (2-DE) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometer (MALDI-TOF MS) analysis] were performed sequentially, from 4 to 48 h post-challenge. Cathelicidin-1 and cathelicidin-2 were detected consistently in milk samples obtained throughout the study, and spot optical densities obtained from PDQuest v.8.0 were recorded. …”
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Approach to Design and Evaluate Digital Tools to Enhance Young Adult Participation in Clinical Trials: Co-Design and Controlled Intercept Study by Tim Mackey, Raphael E Cuomo, Qing Xu, Tiana J McMann, Zhuoran Li, Mingxiang Cai, Christine Wenzel, Joshua S Yang

“…ResultsA significantly higher change in the likelihood of participating in a clinical trial post exposure was observed among study participants when exposed to prototypes of a mobile app (Δ=0.74 on a 10-point scale, P<.01) and website (Δ=0.93, P<.01) compared to those exposed to a Facebook ad (Δ=0.21) but not a digital flyer (Δ=0.58). …”
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Transcriptome analysis reveals the sex-switching mechanism of juvenile hermaphroditism in silver pomfret (Pampus argenteus) by Yaya Li, Jiabao Hu, Chongyang Wang, Man Zhang, Youyi Zhang, Yuanbo Li, Mengke Tang, Chunlai Qin, Zukang Feng, Shanliang Xu, Xiaojun Yan, Xubo Wang, Haimin Chen, Yajun Wang

“…Abstract Background Our previous study on silver pomfret (Pampus argenteus) demonstrated that all gonads initially develop into ovaries by 60 days post-hatch (dph). Between 80 and 120 dph, some oocytes undergo apoptosis, resulting in the development of testes and a transient hermaphroditic stage. …”
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Three-dimensional retrospective analysis of root resorption and incisive canal changes following maxillary incisor retraction in class I and class II extraction cases: a comparison... by Remsh Khaled Al-Rokhami, Karim Ahmed Sakran, Naseem Ali Al-Worafi, Maher Al-balaa, Ahmed Algahefi, Zhihua Li

“…Patients received either clear aligners (n = 33) or fixed appliances (n = 33) following first premolar extraction. Pre- and post-treatment CBCT measurements were recorded at three vertical reference planes (H1, H2, H3: 2 mm, 4 mm, and 6 mm above the labial cementoenamel junction), including IC width, cortical bone width (CBW), U1–IC distance, IC height, and U1 root length/width. …”
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Dynamics of humoral immunity during natural SARS-CoV-2 infection and/or after vaccination with the ‘Sputnik V’ vaccine by A. V. Ivanov, M. A. Uvarova, K. B. Frolov, E. V. Semenova

“…However, both maximal values of anti-SARS-CoV-2 IgA and IgG and the rates of post-vaccination humoral immune response differed significantly between the persons who have previously had COVID-19 and those who have not previously been infected with SARS-CoV-2. …”
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Advancing wastewater reuse: AI-driven insights into ozone-based organic pollutant reduction by Syed Muzzamil Hussain Shah, Sani I. Abba, Mohamed A. Yassin, Ebrahim Al-Qadami, Dahiru U. Lawal, Imtiaz Afzal Khan, Jamilu Usman, Haris U. Qureshi, Isam H. Aljundi

“…The ORP levels, initially 155–165 mV in untreated samples, rose significantly post-ozonation to 208 and 218.30 mV for 15 and 25 min of treatment, respectively while reducing the elevated BOD levels. …”
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Isolation and Identification of Tomato Bacterial Wilt Pathogen (Ralstonia solanacearum) and Screening of Disease-Resistant Germplasm by Mingzhu GUAN, Jialong LI, Xiaojiang SUN, Guang LI, Ziqi LIU, Yueqin ZHANG, Rongchao YANG

“…Both strains exhibited strong pathogenicity, with h4 demonstrating higher virulence. At 4 days and 6 days post-inoculation, disease incidence rates in 'ZS-5' and 'GZ-1' inoculated with h4 reached 67.7% and 4.4% (4 d), 92.2% and 13.3% (6 d), respectively, consistently higher than those of h2. …”
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The influence of altered lower-limb muscle strength on dynamic plantar pressure distribution in participants who underwent anterior cruciate ligament reconstruction by Karolína Matov, Michal Bozděch, Marta Gimunová, Martin Komzák, Marek Dostál, Miloslav Maršálek, Tomáš Vespalec, Milan Mojžíš, Tomáš Vodička

“…Non-parametric Friedman tests with Kendall's W assessed temporal differences, followed by Conover post hoc tests with Bonferroni correction. Spearman's rank correlation examined associations between muscle strength and plantar pressure.ResultsSignificant deficits in extensor strength were found at both postoperative time points compared to preoperative levels (both p &lt; .001), with improvement at six months vs. three (p &lt; .001). …”
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