Combined sequential hypothermic oxygenated and normothermic machine perfusion for liver transplant from an expanded criteria donor: first clinical application in Russia by S. V. Gautier, N. V. Grudinin, A. R. Monakhov, M. A. Boldyrev, V. K. Bogdanov, D. M. Bondarenko, S. I. Zubenko, N. P. Mozheiko, M. G. Minina, O. M. Tsirulnikova

“…Preservation times were: SCS – 260 minutes, D-HOPE – 124 minutes, NMP – 480 minutes, with a total preservation time of 884 minutes. Post-transplant peak levels of AST, ALT, and total bilirubin in the recipient were 922.5 U/L, 613 U/L, and 63.3 μmol/L, respectively. …”
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Goblet cell metaplasia and mucin alterations in biliary epithelial cells during Opisthorchis viverrini infection in rodent models: Insights into host susceptibility and defense mec... by Woro Danur Wendo, Theerayut Thongrin, Prasarn Tangkawattana, Peerapol Sukon, Sutas Suttiprapa, Prasert Saichua, Watcharapol Suyapoh, Sirikachorn Tangkawattana

“…Infected animals received 50 OV metacercariae through gastric intubation and were sacrificed on days 1, 2, 7, 14, 28, and 56 post-infection. Histological, histochemical (Alcian Blue, periodic Acid-Schiff, and high iron diamine), and immunohistochemical (Bromodeoxyuridine [BrdU]) analyses were performed to assess mucin production, GCM, and bile duct proliferation. …”
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Effects of dietary mangosteen peel powder and extract on the growth performance, meat quality and indicators for immunity, gut health and antioxidant activity in broiler chicks by Da-Hye Kim, Hyeon Mo Yang, Ju-Yong Song, Jina Park, Byung-Yeon Kwon, Anh Viet Vu, Dae Sung Lee, Kyung-Woo Lee

“…Data were analyzed using analysis of variance, and post hoc comparisons of treatments were performed using Tukey's Honestly Significant Difference test. …”
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Associations of gut microbiota with disease development, disease activity, and therapeutic effects in patients with systemic lupus erythematosus by Junko Nishio, Hiroshi Sato, Eri Watanabe, Hiroaki Masuoka, Kotaro Aoki, Mai Kawazoe, Risa Wakiya, Soichi Yamada, Sei Muraoka, Shotaro Masuoka, Tomoki Hayashi, Satoshi Mizutani, Zento Yamada, Keiko Koshiba, Izumi Irita, Miwa Kanaji, Karin Furukawa, Nobuyuki Yajima, Hiroaki Dobashi, Wataru Hirose, Yoshikazu Ishii, Wataru Suda, Toshihiro Nanki

“…Abstract Altered gut microbiota is linked to systemic lupus erythematosus (SLE), but its association with disease development, disease activity, and post-intervention changes remains unclear. We compared new-onset SLE (NOSLE, n = 25), SLE in remission (RemSLE, n = 30), and healthy controls (HC, n = 30) cross-sectionally and conducted the first longitudinal analysis of NOSLE patients (n = 22) from pre-intervention to remission over 12 months. …”
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Severe Acute Respiratory Syndrome Coronavirus 2 Variant Infection Dynamics and Pathogenesis in Transgenic K18-h<i>ACE2</i> and Inbred Immunocompetent C57BL/6J Mice by Hongwei Liu, Brianna M. Ramirez, Talia S. Wong, Christopher M. Weiss, Kevin C. K. Lloyd, Qizhi Gong, Lark L. Coffey

“…Following intranasal inoculation with B.1, alpha, beta, or delta variants, K18 mice experienced rapid weight loss and reached euthanasia criteria by 5–6 days post-inoculation (dpi). In contrast, K18 mice inoculated with both omicron variants recovered to their starting weight within 4–6 dpi. …”
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Sex-specific effects of gastrointestinal microbiome disruptions on Helicobacter pylori-induced gastric carcinogenesis in INS-GAS mice by Chao Peng, Xin Li, Yu Li, Xinbo Xu, Yaobin Ouyang, Nianshuang Li, Nonghua Lu, Yin Zhu, Cong He

“…Notably, we found gender disparities in the alterations of gastric and intestinal microbiota in mice post H. pylori infection. While the enrichment of Bifidobacterium and Lachnospiraceae was observed in female mice, Escherichia_Shigella and Akkermansia were more abundant in males. …”
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Physiological and clinical parameters under different protocols of total intravenous anesthesia in llama (Lama glama) undergoing unilateral ovariectomy by Jose Goicochea-Vargas, Marisol Warthon-Medina, Wilson Rondón-Jorge, Fidel Acosta-Pachorro, Marcelo Ratto-Fuster, Mauricio Silva-Jimenez, Ximena Valderrama-Linares, Iain Richards, Max Salvatierra-Alor

“…A drop in SpO2 was seen at minute 5 in all groups, because of respiratory depression post-anaesthetic induction, subsequently the values increased, with better saturation in G2. …”
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α-Synuclein orchestrates Th17 responses as antigen and adjuvant in Parkinson’s disease by Emi Furusawa-Nishii, Bataa Solongo, Kou Rai, Soichiro Yoshikawa, Asako Chiba, Ayami Okuzumi, Shin-ichi Ueno, Yasunobu Hoshino, Yoko Imamichi-Tatano, Haruka Kimura, Taku Hatano, Nobutaka Hattori, Sachiko Miyake

“…Although the existence of αS-specific T cells and the immunogenicity of the post-translationally modified αS fragment have been reported in PD and DLB, the key cellular subset associated with disease progression and its induction mechanism remain largely unknown. …”
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Antisense oligonucleotides targeting the SARS-CoV-2 nucleocapsid gene decrease viral titers in hamsters by Christopher Fitzpatrick, Nicolas Meunier, Sophie Lepoder, Sophie Dhorne-Pollet, Bruno Da Costa, Bernard Klonjkowki, Jean-François Eléouët, Verónica A. Burzio, Bernard Delmas, Eric Barrey

“…ASO-N1 induced a sustained reduction in viral RNA and inflammatory cytokine expression in the nasal mucosa and efficiently decreased infectious viral titers in nasal swabs at day 3 post infection. Further optimization of the ASO-N1 sequence with a combination of RNase-H1-dependent and steric blockage chemistries synergistically increased the antiviral efficacy in vitro. …”
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From inconsistency to sustainability: ICU night huddle compliance improvement through a structured QI approach by Prasobh PV Mukundan, Alisha Karki

“…Materials and methods: This QI project followed a plan–do–study–act (PDSA) cycle over two phases: (1) initial QIP (September 2023–March 2024): a structured Night Shift Briefing Checklist was implemented to ensure standardised communication and that compliance was monitored; (2) re-audit (September 2024–December 2024): targeted interventions were implemented to address compliance gaps:• Phase 1: Checklist placed in Doctors’ Hub (22 September 2024)• Phase 2: WhatsApp group reminders to doctors (1 October 2024)• Phase 3: Message of the Week in Morning Huddle (14 October 2024)• Phase 4: Nursing In-Charge Involvement (15 November 2024)Weekly compliance rates were recorded and analysed pre- and post-interventions. Results and discussion: Compliance improved from 32.8% (initial QIP) to 70.7% (re-audit), a 37.9% increase (Fig 1).The impact of each intervention was assessed as follows: • Phase 1: Checklist in Doctors’ Hub (22 September 2024): initial compliance was low, with inconsistent adherence. • Phase 2: WhatsApp reminders (1 October 2024): compliance increased to ∼57%, but some nights were still missed due to lack of structured accountability. • Phase 3: Message of the Week in Morning Huddle (14 October 2024): reinforcement through daily reminders resulted in a boosted compliance of ∼71%. • Phase 4: Nursing In-Charge Involvement (15 November 2024): sustained compliance of ∼75% was achieved by actively engaging nursing staff, ensuring a shared responsibility rather than relying solely on doctors.Challenges: night huddle non-compliance persisted due to: • Workload pressure – If ICU was busy, staff may have skipped the huddle; • Forgetting/skipping: some days were missed even with reminder; • No direct accountability: night huddle often relied on motivated individuals; • Staff rotation issues: new staff may not have been fully aware of the expectation; • Nursing–doctor coordination issues: some shifts lacked direct interaction.What can be done to sustain and improve compliance? …”
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Intimate partner violence among lesbian, bisexual, and queer women students on campuses in South Africa: a qualitative study exploring context, drivers, and impacts by Pinky Mahlangu, Carrie Brooke-Sumner, Yandisa Sikweyiya, Kristin Dunkle, Ntlotleng Mabena, Rachel Jewkes, Sinegugu Duma, Nelisiwe Khuzwayo, Elizabeth Dartnall, Managa Pillay, Mercilene Tanyaradzwa Machisa

“…Data were analyzed inductively using a thematic analysis approach. We drew from the post-structural feminist theory to understand the relationship dynamics in same sex relationships of LBQ women. …”
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Investigation of Lactobacillus Probiotics Derived from Traditional Dairy Products in Eliciting Anti-Tumor Responses in Mouse Colorectal Cancer Model by Shaghayegh Rezai, Elnaz Ghorbani, Seyedeh Elnaz Nazari, Farzad Rahmani, Seyed Mahdi Hassanian, Asma Afshari, Mohammad Bagher Habibi Najafi, Amir Avan, Mikhail Ryzhikov, Saman Soleimanpour, Majid Khazei

“…The tests employed included the Kolmogorov–Smirnov, ANOVA, Dunnett’s post hoc, and Kruskal–Wallis.Results: Lactobacillus strains effectively suppressed tumor growth in CRC by promoting cell death and inhibiting fibrosis and inflammation. …”
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Anti-BCL2 therapy eliminates giant congenital melanocytic nevus by senolytic and immune induction by Boxuan Wei, Qingxiong Yu, Jiamin Jin, Danli Zhu, Bohan Lai, Jieyu Gu, Ran Yang, Huailiang Huang, Hongzhan Lin, Liang Zhang, Tao Zan, Feng Xie, Kang Zhang, Qingfeng Li

“…Neutrophil depletion and immunosuppression reduce treatment efficacy, highlighting the crucial role of the immune response post-BCL2i treatment. Long-term follow-up showed no recurrence, with neutrophils and T cells residing in the dermis, indicating memory immune reactions. …”
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Study on the Relationship between Swallowing-Related Muscle Activity and Respiratory Muscle Function in Stroke Patients with Dysphagia by SU Panpan, HE Chuan, FENG Min, JIA Yuanyuan, WANG Tiantian, MENG Dianhuai

“…The root mean square and peak amplitude of the affected submental muscles, the healthy submental muscles and the affected infrahyoid muscles were positively correlated with MEP (<italic>r</italic>=0.534, <italic>r</italic>=0.595, <italic>r</italic>=0.332, <italic>r</italic>=0.455, <italic>r</italic>=0.323, <italic>r</italic>=0.375, <italic>P</italic>&lt;0.05).ConclusionPatients with post-stroke dysphagia who have respiratory muscle weakness exhibit a significant decrease in the strength of the submental muscle group, and there is a correlation between swallowing-related muscle group activity and respiratory muscle function in stroke patients with dysphagia.…”
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La transformation de l’ancien centre de détention secrète de Derb Moulay Cherif en musée des « années de plomb » (Casablanca, Maroc) by Anissa Habane

“…The first concerns the establishment of former secret detention centers in the post-independent Moroccan kingdom, enabling us to grasp this painful, shameful and dark period in the country’s history. …”
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Which novel teaching strategy is most recommended in medical education? A systematic review and network meta-analysis by Shuai-Long Zhang, Si-Jing Ren, Dong-Mei Zhu, Tian-Yao Liu, Lian Wang, Jing-Hui Zhao, Xiao-Tang Fan, Hong Gong

“…Therefore, this systematic review and network meta-analysis aims to evaluate and prioritize various teaching strategies in medical education, including simulation-based learning (SBL), flipped classrooms (FC), problem-based learning (PBL), team-based learning (TBL), case-based learning (CBL), and bridge-in, objective, pre-assessment, participatory learning, post-assessment, and summary (BOPPPS). Methods We conducted a comprehensive systematic search of PubMed, Embase, Web of Science, the Cochrane Library, and some key medical education journals up to November 31, 2023. …”
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A Culturally Tailored mHealth Intervention (MobileMen App) to Promote Physical Activity in African American Men: Protocol for a Comparative Effectiveness Trial by Kayla Nuss, Amanda Brice, Callie Hebert, Phillip Nauta, April J Stull, Damon L Swift, Derek M Griffith, David B Buller, Robert L Newton Jr

“…Participants will complete assessments at baseline and at 6 months post randomization. Assessments include objective measurements of daily steps and minutes of moderate to vigorous PA, quality of life, dietary measures, self-efficacy for fruit and vegetable consumption and PA, and autonomous motivation for PA. …”
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Transcriptome sequencing–based analysis of the molecular mechanism underlying the effect of lncRNA AC003090.1 on osteoporosis by Huafeng Zhuang, Yongjun Lin, Chengye Lin, Miao Zheng, Yizhong Li, Xuedong Yao, Youjia Xu

“…The expression levels of Runx2, Opn, and Ocn in spongy and cortical bone samples, as well as in post-infection cells, were assessed through RT-qPCR. …”
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Static stress triggering of Morocco M 6.9 earthquake on 9 September 2023 by WANG Runyan, WAN Yongkui, GUAN Zhaoxuan, HUANG Shaohua, GU Peiyuan, SONG Zeyao, ZHOU Mingyue

“…Therefore, an in-depth exploration of earthquake triggering is of great significance for strengthening post-earthquake scientific research and improving society's ability to respond to earthquakes.…”
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COVID-19 and common mental health symptoms in the early phase of the pandemic: An umbrella review of the evidence. by Anke B Witteveen, Susanne Y Young, Pim Cuijpers, José Luis Ayuso-Mateos, Corrado Barbui, Federico Bertolini, Maria Cabello, Camilla Cadorin, Naomi Downes, Daniele Franzoi, Michael Gasior, Brandon Gray, Maria Melchior, Mark van Ommeren, Christina Palantza, Marianna Purgato, Judith van der Waerden, Siyuan Wang, Marit Sijbrandij

“…<h4>Methods and findings</h4>A systematic search was carried out in 5 databases for peer-reviewed systematic reviews with meta-analyses of prevalence of depression, anxiety, and post-traumatic stress disorder (PTSD) symptoms during the pandemic published between December 31, 2019 until August 12, 2022. …”
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