Generation and evaluation of a novel PI3K-targeting gene therapy in the failing mouse heart and healthy sheep heart by Sebastian Bass-Stringer, Daniel G. Donner, Clive N. May, Aya Matsumoto, Emma I. Masterman, Aascha A. D'Elia, Yi Ching Chen, Helen Kiriazis, Jieting Luo, Roger Chooi, Clara Liu Chung Ming, Paul Gregorevic, Colleen J. Thomas, Bianca C. Bernardo, Kate L. Weeks, Julie R. McMullen

“…This AAV (1 × 1012, 2 × 1012 vg) was administered to mice with myocardial ischemia/reperfusion injury (I/R: 1 h ischemia with reperfusion; AAV delivered 24 h post-I/R). Direct cardiac injections of PI3K-based AAVs were also performed in healthy adult female sheep. …”
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Mesocorticolimbic dopaminergic innervation modulates Levodopa-related improvements in gait in older slow walking Adults: A [11C]PE2I PET study by N. Paalanen, S. Roytman, M. van Emde Boas, A. Biddix, A. Luker, F. Michalakis, G. Carli, P. Kanel, C. Pongmala, R. Koeppe, L.M. Chahine, C. Rosano, N.I. Bohnen

“…Spatiotemporal gait parameters were evaluated at pre-intervention, following carbidopa pre-treatment, and post-intervention. Results: Levodopa treatment resulted in improved gait speed (β = 0.69 [0.07, 1.30], p = 0.031), double-support time (β = -0.26 [-0.51, −0.01], p = 0.041), and cadence (β = 0.54 [0.18, 0.89], p = 0.005), but not in turn duration (β = 0.41 [-0.33, 1.14], p = 0.264). …”
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Community burden of acute respiratory infections in Shanghai, a longitudinal cohort study in respiratory pathogens, China, 2024–2027 by Ting Zhang, Yan Luo, Li Zhang, Xiao Wang, Yifeng Shen, Yu Li, Zhongjie Li, Bing Zhao, Benjamin J Cowling, Chuchu Ye, Jianxing Yu, Lance Rodewald, Weizhong Yang, Xinmei Zhou, Ling Xin, Xuya Yu, Yanxin Xie, Yilin Jia, Linghui Zhao, Yaoyao Wang, Hualei Xin, Lipeng Hao, Lili Ren

“…Illness courses and clinical recoveries of ARI cases are assessed through weekly contact with ARI cases for 28 days post ascertainment.Findings to date Between 14 October 2024 and 22 November 2024, we enrolled 5387 community residents into the cohort, including 233 children aged from 6 months to 2 years, 278 preschool children aged 3–6 years, 575 school-age children aged 7–18 years, 2150 adults aged 19–64 years and 2151 older adults aged 65+years. …”
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Optimising cardiac surgery outcomes in people with diabetes: the OCTOPuS pilot feasibility study by Richard IG Holt, Katharine Barnard-Kelly, Mayank Patel, Philip Newland-Jones, Suvitesh Luthra, Jo Picot, Helen Partridge, Andrew Cook

“…Two important uncertainties in the management of people with diabetes undergoing major surgery exist: (1) how to improve diabetes management prior to an elective procedure and (2) whether that improved management leads to better post-operative outcomes. Objective The Optimising Cardiac Surgery ouTcOmes in People with diabeteS project aimed to assess whether a pre-operative outpatient intervention delivered by a multidisciplinary specialist diabetes team could improve diabetes management and cardiac surgical outcomes for people with diabetes. …”
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Multicentre pragmatic embedded stepped wedge cluster randomised trial comparing glucose 5% with sodium chloride 0.9% as the default drug diluent in the ICU: the sweet-water trial p... by Alexander Zarbock, Kai-Uwe Eckardt, Claudia Spies, Felix Balzer, Martin Witzenrath, Philipp Enghard, Björn Tampe, Oliver Hunsicker, Steffen Weber-Carstens, Bjoern Weiss, Jens Nee, Christian Strauss, Heiko Schenk, Jan-Hendrik Bernhard Hardenberg, Ricarda Merle Hinz, Mareen Pigorsch, Alexander Uhrig, Holger Müller-Redetzky, Tim Schroeder, Ulrike Trost, Daniel Zickler, Rainer Borgstedt, Mariam Abu-Tair, Uta Hildebrand, Dario von Wedel

“…Hypernatraemia adversely affects various physiological functions and is associated with delirium, prolonged length of stay and increased ICU and post-discharge mortality. The sodium load from intravenous drug diluents significantly contributes to ICU-acquired hypernatraemia, with drug infusions comprising about 30% of the daily fluid volume of an average ICU patient. …”
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Reduced morbidity and mortality of cGVHD in patients who received treatment with mesenchymal stromal cells for steroid-resistant aGVHD: long-term follow-up of a randomized phase 3... by Ke Zhao, Ren Lin, Zhiping Fan, Zhen Li, Xiaoyong Chen, Li Xuan, Fen Huang, Na Xu, Xiuli Wu, Shaohua Chen, Jing Sun, Xi Zhang, Jianyu Weng, Yonghua Li, Yuhua Li, Dongjun Lin, Danian Nie, Shunqing Wang, Xiaojun Xu, Xiaohui Zhang, Yangqiu Li, AP Xiang, Yu Wang, Qifa Liu

“…Abstract Background Our open-label, multicenter, randomized, phase 3 trial showed that the incidence and severity of chronic graft-versus-host disease (cGVHD) reduced in steroid-resistant acute graft-versus-host disease (aGVHD) patients who underwent mesenchymal stromal cells (MSCs) treatments, but survival benefit was not received. Here, we present a post-hoc analysis of the 5-year follow-up to explore long-term survival and its underlying mechanism. …”
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Target Trial Emulation of the Modified Vaccinia Ankara-Bavarian Nordic Vaccine for Pre-Exposure Mpox Prevention in At-Risk Populations by Clara Suñer, Roser Escrig-Sarreta, Cristina Galván-Casas, Eduardo Matos, Amanda Gabster, Marcelo Wolff, Dan Ouchi, Andrea Alemany, Hugo Sánchez, Sandra Huaman, Dixennia Bejarano, Lourdes Carrés-Esteve, Cristina Santiago-Fernández, Javier Corral-Rubio, Adrià Mendoza, Àngel Rivero, Vicente Descalzo, Eva Orviz, Héctor Martínez-Riveros, Leonardo Méndez-Boo, Carmen Cabezas, Araceli Arce-Arnáez, Michael Marks, Oriol Mitjà, REMAIN Study Group

“…The primary endpoint was symptomatic mpox occurrence ≥14 days post-vaccination. <b>Results</b>: The primary analysis included 1028 individuals (514 vaccinated, 514 unvaccinated) with a median follow-up time of 9.3 months (IQR 4.7–13.7). …”
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Evaluation of Treatment Response in Chronic Hepatitis C Patients Receiving Sofosbuvir/Velpatasvir/Voxilaprevir: A Multicenter Real-World Experience from Türkiye by Umut Devrim Binay, Faruk Karakeçili, Bilgehan Aygen, Ayşin Kılınç Toker, İlhami Çelik, Neşe Demirtürk, Tuğçe Şimşek Bozok, Leyla Dursun, Fethiye Akgül, Güle Çınar, Özgür Günal, Ali Asan, Eyüp Arslan, Fatma Yılmaz Karadağ, Orçun Barkay, İrem Akdemir, Funda Şimşek, Emine Türkoğlu Yılmaz, Zeynep Ravza Eğilmez, Süda Tekin, The Viral Hepatitis Study Group of the Turkish Society of Clinical Microbiology and Infectious Diseases (KLİMİK)

“…Among the 80 patients for whom week-12 post-treatment data were available, 97.5% sustained virologic response at week 12 (SVR12). …”
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Impact of the COVID-19 pandemic on antidepressant prescribing with a focus on people with learning disability and autism: an interrupted time series analysis in England using OpenS... by Ben Goldacre, David Evans, Orla Macdonald, Richard Croker, Rebecca Smith, Christopher Wood, Andrea Schaffer, Andrew Brown, Brian MacKenna, William Hulme, Chris Bates, Amir Mehrkar, Peter Inglesby, John Parry, Amelia Green, Helen Curtis, Alex Walker, Rose Higgins, Sebastian Bacon, Simon Davy, Tom Ward, Louis Fisher, Iain Dillingham, Milan Wiedemann, Lucy Bridges, Ben Butler-Cole, Colm Andrews, Christine Cunningham, Victoria Speed, Liam C Hart, Thomas O’Dwyer, Steve Maude

“…We did not find evidence that the pandemic was associated with a change in antidepressant prescribing trend in the general population (relative risk (RR) 1.00 (95% CI 0.97 to 1.02)), in those with autism (RR 0.99 (95% CI 0.97 to 1.01)) or in those with learning disability (RR 0.98 (95% CI 0.96 to 1.00)).New prescribing post restrictions was 13% and 12% below expected had COVID-19 not happened in both the general population and those with autism (RR 0.87 (95% CI 0.83 to 0.93), RR 0.88 (95% CI 0.83 to 0.92)), but not learning disability (RR 0.96 (95% CI 0.87 to 1.05)).Conclusions and implications In this England study, we did not see an impact of COVID-19 on overall antidepressant prescribing, although unique trends were noted, such as trends in new antidepressant prescriptions which increased in care homes over the pandemic and decreased in the general population and those with autism since recovery.…”
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Impacto del alargamiento de miembros inferiores con clavos telescópicos en la funcionalidad y calidad de vida de pacientes con acondroplasia by M. Galán-Olleros, J. Alonso-Hernández, C. Miranda-Gorozarri, J. García-Fernández, R.M. Egea-Gámez, Á. Palazón-Quevedo

“…Functionality (Lower Extremity Functional Scale, LEFS), self-esteem (Rosenberg Self-Esteem Scale), and HRQoL (Short Form-12, SF-12, and EuroQol VAS) were assessed preoperatively and at least two years post-surgery. Complications (Clavien-Dindo-Sink classification) and patient satisfaction were also recorded. …”
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Long-term alteration of heart rate variability following childhood maltreatment: Results of a general population study by Marian Stürmer, Johanna Klinger-König, Marcus Vollmer, Antoine Weihs, Stefan Frenzel, Marcus Dörr, Lars Kaderali, Stephan B. Felix, Beate Stubbe, Ralf Ewert, Henry Völzke, Hans J. Grabe, Elischa Krause

“…HRV was derived from 10-second electrocardiograms and 5-minute pre-sleep polysomnographic recordings. Post hoc analyses examined abuse and neglect. Results CM was associated with reduced HRV (logRMSSD: β = −0.20 [95%-CI: −0.28, −0.12], p = 1.2e−06), driven by neglect (β = −0.27 [−0.35, −0.18], p = 1.9e−09) rather than abuse (β = 0.01 [−0.12, 0.14], p = 1). …”
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Late anastomotic complication after laparoscopic surgery for clinical stage I low rectal cancers located within 5 cm of the anal verge: Sub‐analysis of the ultimate trial by Manabu Shimomura, Masanori Yoshimitsu, Yuichiro Tsukada, Hideki Ohdan, Jun Watanabe, Yosuke Fukunaga, Yasumitsu Hirano, Kazuhiro Sakamoto, Hiroki Hamamoto, Hisanaga Horie, Nobuhisa Matsuhashi, Yoshiaki Kuriu, Shuntaro Nagai, Madoka Hamada, Shinichi Yoshioka, Shinobu Ohnuma, Tamuro Hayama, Koki Otsuka, Yusuke Inoue, Kazuki Ueda, Yuji Toiyama, Satoshi Maruyama, Shigeki Yamaguchi, Keitaro Tanaka, Motoko Suzuki, Toshihiro Misumi, Takeshi Naitoh, Masahiko Watanabe, Masaaki Ito, Ultimate Trial Group

“…Abstract Background Although extensive research has been conducted on early anastomotic leakage (AL) after sphincter‐sparing surgery, the status of late anastomotic complications (post‐30 days) has received limited attention. These late complications significantly affect a patient's quality of life and often lead to permanent stoma creation. …”
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Urban greening for climate resilient and sustainable cities: grand challenges and opportunities by Prashant Kumar, Prashant Kumar, Prashant Kumar, Jeetendra Sahani, Karina Corada Perez, Ajit Ahlawat, Ajit Ahlawat, Maria de Fatima Andrade, Maria Athanassiadou, Shi-Jie Cao, Shi-Jie Cao, Lisa Collins, Sagnik Dey, Sagnik Dey, Silvana Di Sabatino, Christos H. Halios, Felicity Harris, Colin Horton, Luis Inostroza, Luis Inostroza, Laurence Jones, Laurence Jones, Thomas Rodding Kjeldsen, Ben McCallan, Ben McCallan, Aonghus McNabola, Aonghus McNabola, Rajeev Kumar Mishra, Sumit Kumar Mishra, Sumit Kumar Mishra, Sumit Kumar Mishra, Erick G. Sperandio Nascimento, Erick G. Sperandio Nascimento, Erick G. Sperandio Nascimento, Gwilym Owen, Khaiwal Ravindra, Neyval C. Reis, Veronica Soebarto, Jannis Wenk, Jannis Wenk, Hannah Sloan Wood, Runming Yao, Runming Yao, Runming Yao

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Antibodies against integrin αvβ6 have high diagnostic accuracy for ulcerative colitis by Patrick Bez, Martina Scapolan, Davide Giuseppe Ribaldone, Gian Paolo Caviglia, Silvia Zago, Cristina Trucco, Simone Frara, Antonino Caruso, Marta Ascolani, Michele Campigotto, Stefano Benvenuti, Stefano Martelossi, Lara De Lucchi, Marta Favero, Francesco Cinetto, Francesco Cinetto, Elisabetta Faggin, Laura Ventura, Marcello Rattazzi, Marcello Rattazzi, Tilde Manetta, Giulio Mengozzi, Antonio Antico, Carla Felice, Carla Felice

“…The UC patients presented higher values of anti-integrin αvβ6 IgG compared to CD, IBS, and controls (Kruskal–Wallis test and post-hoc Holm’s correction: p &lt; 0.001). The ROC of anti-integrin αvβ6 IgG performed optimally with an area under the curve of 0.93. …”
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Hyperspectral abdominal laparoscopy with real-time quantitative tissue oxygenation imaging: a live porcine study by Oscar MacCormac, Oscar MacCormac, Conor C. Horgan, Conor C. Horgan, Dale Waterhouse, Dale Waterhouse, Philip Noonan, Philip Noonan, Mirek Janatka, Mirek Janatka, Richard Miles, Richard Miles, Jaco Jacobs, Cameron Dockerill, Cameron Dockerill, Théo Trotouin, Théo Trotouin, Alexis Schizas, Barbara Seeliger, Barbara Seeliger, Barbara Seeliger, Sebastien Ourselin, Sebastien Ourselin, Michael Ebner, Michael Ebner, Tom Vercauteren, Tom Vercauteren, Jonathan Shapey, Jonathan Shapey, Jonathan Shapey

“…While intra-operative overt ischaemia can be perceived with the naked eye, timely recognition of borderline perfusion can prevent post-operative ischaemic complications, which is particularly relevant for colorectal anastomoses. …”
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Restoration of NOX4 signalling reverses endothelial colony-forming cell angiogenic dysfunction associated with experimental and clinical diabetes by Karla M. O’Neill, Kevin S. Edgar, Shun Hay Pun, David C. Campbell, Tinrui Toh, Xin N. Wong, Bianca Botezatu, Jyoti Kandel, Una McCoy, Jennifer Nicell, Catherine McClintock, Kiran J. McLoughlin, Yuxin Wu, Vinuthna Vani Madishetti, Arya Moez, Mohammed Alsaggaf, Eleanor K. Gill, Rawan A. Abudalo, Christina L. O’Neill, Edoardo Pedrini, Jasenka Guduric-Fuchs, Coy Brunssen, Henning Morawietz, Philip D. Dunne, Chris J. Watson, Reinhold J. Medina, David J. Grieve

“…Statistical significance was determined using one-way ANOVA with Bonferroni post-host testing or paired/unpaired Student’s t-test, as appropriate. …”
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Études comportementales pour la conservation du singe laineux de Poeppig (Lagothrix l. poeppigii) by Justine Philippon

“…Le but sera ici de savoir si le traumatisme lié à la capture et à la séparation subite du groupe natal produit des effets comparables à ceux du choc post-traumatique observés chez l’homme et les chimpanzés. …”
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Soft Tissue Augmentation by Electrospun Membranes embedded With Nano-Hydroxyapatite: Histologic and Volumetric Analyses by Yu Zhu, Zuohui Xiao, Yingxin Gu, Qiang Zhi, Zhuoli Huang, Yijie Zhang, Chunan Zhang, Shichong Qiao

“…The animals were sacrificed at 12 weeks post-surgery and received volumetric analyses. Histological examinations at the meantime were carried out at five different depths below the crest (0, 1.5, 2.5, 3.5 and 5.5 mm). …”
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Assessment and Enhancement of Coastal Community Resilience Strategies: A Case Study of Wuyuan Bay in Xiamen by Xu Mengjie, Liu Xingzhao, Xie Huili, Zhang Yang, Dai Hongxia, Zhou Yanhai, Huang Faming

“…Compared with traditional disaster prevention models, the synergistic mechanism between resilience theory and community risk management not only provides a theoretical framework for urban complex risk prevention, but also demonstrates dynamic adaptive advantages in pre-disaster prevention, disaster response, and post-disaster recovery. Accordingly, this study integrated the resilience community theory with the sponge city concept, selecting 25 storm surge-prone bay communities in the Xiamen Wuyuan Bay Area as samples to establish a community resilience evaluation framework encompassing exposure, vulnerability, adaptability, and spatial connectivity. …”
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Short-Term Hormonal Changes Following Microdissection Testicular Sperm Extraction among Men with Non-Obstructive Azoospermia: Findings from a Large Longitudinal Prospective Multice... by Fausto Negri, Edoardo Pozzi, Christian Corsini, Massimiliano Raffo, Federico Belladelli, Alessandro Bertini, Francesco Cattafi, Eugenio Ventimiglia, Rayan Matloob, Antonino Saccà, Luca Boeri, Alessia d’Arma, Francesco Montorsi, Andrea Salonia

“…Preoperative serum hormone levels (total testosterone [tT], luteinizing hormone [LH], follicle-stimulating hormone [FSH], and 17β-estradiol) were collected, with repeat measurements at 3-month post-surgery. We divided the cohort into two groups using a tT cut-off value of 3 ng/mL: 1) men who kept eugonadal status; and, 2) men who were initially eugonadal but became testosterone deficient (TD) after surgery. …”