Molecular Mechanism for PACAP 38-Induced Neurite Outgrowth in PC12 Cells by Junko Shibato, Fumiko Takenoya, Takahiro Hirabayashi, Ai Kimura, Michio Yamashita, Ichiro Takasaki, Randeep Rakwal, Seiji Shioda

“…PC12 cells were treated with PACAP38 being added to the cell culture medium at concentrations of 10−7 M, 10−8 M, and 10−9 M. Post PACAP38 treatment, immunostaining was used to confirm protrusion elongation of the PC12 cells, while RT-PCR, two-dimensional gel electrophoresis in conjunction with Western blotting, and inhibition experiments were performed to confirm the expression of the PACAP gene, its receptors, and downstream signaling components. …”
Get full text

The effect of empagliflozin on peripheral microvascular dysfunction in patients with heart failure with preserved ejection fraction by Sanne G. J. Mourmans, Anouk Achten, Raquel Hermans, Marijne J. E. Scheepers, Elisa D’Alessandro, Geertje Swennen, Janneke Woudstra, Yolande Appelman, Harry van Goor, Casper Schalkwijk, Christian Knackstedt, Jerremy Weerts, Etto C. Eringa, Vanessa P. M. van Empel

“…Methods This is a pre-post intervention study in patients diagnosed with HFpEF who are eligible for treatment with empagliflozin. …”
Get full text

Clinical efficacy and regulatory mechanisms of Shi Pi Zeng Ye formula in treating functional constipation comorbid with depression: integrating clinical observation, mass spectrome... by Ling Yao, Xiaoqiang Jia, Yufei Li, Haixia Li, Zhuhui Zhang, Longfang Quan, Qiuling Liu, Jie Dai, Xuedi Lei, Huashang Li, Yonghai Li

“…Assessments were conducted at baseline, 4 weeks, and 12 weeks post-treatment. The study also explored the action mechanisms of SPZY through mass spectrometry, network pharmacology, and molecular docking to ascertain the binding affinities of SPZY’s active components to critical targets.ResultsThe study findings indicated significant improvements in WCS (p < 0.0001), HAMD-17 (p < 0.0001), PAC-QOL (p < 0.0001), and HAMA (p < 0.001) scores from baseline to 3 months. …”
Get full text

A pilot study investigating the effect of pembrolizumab on the tumoral immunoprofile of newly diagnosed mullerian cancers by Stéphanie L. Gaillard, Gloria Broadwater, Andrew Berchuck, William S. Andrews, Laura J. Havrilesky, Brittany A. Davidson, Rebecca A. Previs, Mark D. Starr, John S. Yi, Andrew B. Nixon, Jadee L. Neff, Jose Ramon Conejo-Garcia, Angeles Alvarez Secord

“…TIL levels changed in 4 of 11 paired sets, with 3 decreasing and 1 increasing post-treatment. PD-L1 modified proportion score increased in 7 cases, decreased in 2, and remained unchanged in 2. …”
Get full text

Concurrent training and intermittent fasting reduce transverse cross-sectional area of cardiomyocytes and body mass in Wistar rats by Henrique Izaias Marcelo, Henrique Izaias Marcelo, Marina Politi Okoshi, Letícia Estevam Engel, Wanderson da Silva Rosa, Paulo Henrique Aleixo, Paulo Henrique Aleixo, Guilherme Akio Tamura Ozaki, Everton Alex Carvalho Zanuto, Luiz Carlos Marques Vanderlei, Francis Lopes Pacagnelli, Francis Lopes Pacagnelli, Robson Chacon Castoldi

“…After obtaining the data, the Shapiro-Wilk test was performed, followed by a two-way analysis of variance (ANOVA) with Tukey’s post-test for the variables measured at the end of the experiment.ResultsFor the variables body mass, feed consumption, and the anaerobic threshold, the FC group showed a greater decrease in the Lee index [(0.28 ± 0.00) p < 0.05)] in relation to the other groups. …”
Get full text

Ecological studies of different plant communities of Malasaid Hills, District Bajour, Khyber Pukhtoonkhwa, Pakistan by Shakir Ullah, Shahab Ali, Lubna Shakir, Sanam Asif, Ghani Subhan, Mohammad Sohail, Sajid Ali, Yaseen Khan

Get full text

The de novo design and synthesis of yeast chromosome XIII facilitates investigations on aging by Chun Zhou, Yun Wang, Yikun Huang, Yongpan An, Xian Fu, Daqian Yang, Yilin Wang, Jintao Zhang, Leslie A. Mitchell, Joel S. Bader, Yizhi Cai, Junbiao Dai, Jef D. Boeke, Zhiming Cai, Zhengwei Xie, Yue Shen, Weiren Huang

“…We establish causal links between genotypic change and the long-lived phenotype via reconstruction of some key structural variations observed in post-SCRaMbLE strains and further demonstrate combinatorial effects of multiple aging regulators on lifespan extension. …”
Get full text

Engineered small extracellular vesicles for targeted delivery of perlecan to stabilise the blood–spinal cord barrier after spinal cord injury by Wei Peng, Wentao Zhang, Wei Cui, Wenjin Chen, Yin Zhuang, Rupeng Chu, Jinghua Tan, Jingbo Xue, Yiguo Yan, Guoyong Yin, Shujun Zhang, Yong Xie

“…Finally, we identified M2‐sEV‐derived perlecan as a key player in regulating BSCB integrity and functional recovery post‐SCI. Conclusion Our results indicate that RGD‐M2‐sEVs promote BSCB restoration by transporting perlecan to neovascular endothelial cells, representing a potential strategy for SCI treatment. …”
Get full text

Blood Pressure Variability and Outcome Predictors for Traumatic Brain Injury Patients with Diffuse Axonal Injury: A Retrospective Cohort Study by Christine E. Ren, Anastasia Ternovskaia, Fatima Mikdashi, Hassan Syed, Isha Vashee, Vainavi Gambhir, Natalie Chao, Jessica V. Downing, David Dreizin, Quincy K. Tran

“…We investigated whether 24-hour post-injury BPV and other clinical factors were linked to patient outcomes. …”
Get full text

Multimodal CRISPR screens uncover DDX39B as a global repressor of A-to-I RNA editing by Tianzi Wei, Jiaxuan Li, Xiang Lei, Risheng Lin, Qingyan Wu, Zhenfeng Zhang, Shimin Shuai, Ruilin Tian

“…Summary: Adenosine-to-inosine (A-to-I) RNA editing is a critical post-transcriptional modification that diversifies the transcriptome and influences various cellular processes, yet its regulatory mechanisms remain largely unknown. …”
Get full text

Aromatic Amino Acids: Phenylalanine and Tyrosine in Patients with Hypertension and Coronary Artery Disease by A. V. Krivova, M. V. Kozhevnikova, E. O. Korobkova, V. Yu. Zektser, E. A. Zheleznykh, А. A. Ageev, N. E. Moskaleva, A. V. Kukharenko, S. A. Appolonova, Yu. N. Belenkov

“…A statistically significant difference in the level of concentration of Phe and Tyr was revealed (p=0,002 and p=0,024, respectively), comparing the three groups. Post-hoc analysis showed differences in the circulating level of both amino acids in patients with CAD vs the control group (Phe p=0,008 and Tyr p=0,020). …”
Get full text

The Heartline Experience by Janeta Nikolovski, PhD, Ann Marie Navar, MD, Steven Steinhubl, MD, Katie Baca-Motes, MBA, Anne B. Curtis, MD, Eric D. Peterson, MD, Dhanunjaya Lakkireddy, MD, Michael Tarino, MBA, Stephanie Juan, MPH, Chandrasekhar Rao Venkata Damaraju, PhD, Dereck Wentworth, PharmD, Kevin Wildenhaus, PhD, Mithun Patel, MD, MSc, Cammie Tavakoli, BA, C. Michael Gibson, MD, John A. Spertus, MD, MPH

“…Participants interfaced with the study via a custom, app-based engagement program designed to facilitate participant enrollment, post-IRN follow-up, and electronic patient-reported outcome (ePRO) collection. …”
Get full text

Development of Smart pH-Sensitive Collagen-Hydroxyethylcellulose Films with Naproxen for Burn Wound Healing by Elena-Emilia Tudoroiu, Mădălina Georgiana Albu Kaya, Cristina Elena Dinu-Pîrvu, Lăcrămioara Popa, Valentina Anuța, Mădălina Ignat, Emilia Visileanu, Durmuș Alpaslan Kaya, Răzvan Mihai Prisada, Mihaela Violeta Ghica

“…Films presented a biphasic naproxen release profile with more than 75% of the drug released after 24 h, properly managing inflammation and pain on the first-day post-burn. The pH variation mimicking the stages of the healing process demonstrated the color transition from yellow (pH 5.5) to orange (pH 7.4) and finally to bright fuchsia (pH 8.5), enabling easy visual evaluation of the wound environment. …”
Get full text

PROTECTION OF PERSONAL DATA FROM CYBER THREATS by Elizaveta Zainutdinova

“…The study proposes both compliance and post-control measures aimed at preventing breaches and addressing their root causes. …”
Get full text

In-person vs mobile app facilitated life skills education to improve the mental health of internally displaced persons in Nigeria: protocol for the RESETTLE-IDPs cluster randomized... by Ejemai Eboreime, Chisom Obi-Jeff, Rita Orji, Tunde M Ojo, Ihoghosa Iyamu, Bala I Harri, Jidda M Said, Funmilayo Oguntimehin, Abdulrahman Ibrahim, Omolayo Anjorin, Andem Effiong Etim Duke, Umar Baba Musami, Linda Liebenberg, Raquel Crider, Lydia Wagami, Asmau MC Dahiru, Jesse C. Uneke, Sanni Yaya, Vincent IO Agyapong

“…Intervention effectiveness outcomes include the primary outcome of change in post-traumatic stress disorder (PTSD) symptoms assessed using the PCL-5 scale, and secondary outcomes of depression, anxiety, well-being, and life skills acquisition. …”
Get full text

Optimized Sampling Strategies for Isoniazid in East Asian Pediatric Populations Using Population Pharmacokinetics-Informed Approaches by Ju G, Liu X, Peng Y, Yang W, Xu N, He Q, Zhang C, Chen L, Yang N, Zhang G, Li C, Su P, Zhu X, Ouyang D

“…The optimized clinical sampling strategy involved collecting samples at 0.25 [0– 0.5], 1.5 [1– 2], 6 [3– 8], 12 [9– 14], and 24 [22– 24] hours post-dose. Simulation verification showed that re-estimated major PK parameters had acceptable relative biases and relative standard error (< 30%).Conclusion: Traditional adult sampling strategies are inadequate for East Asian pediatric populations. …”
Get full text

Occurrence and Reasons for On-Farm Emergency Slaughter (OFES) in Northern Italian Cattle by Francesca Fusi, Camilla Allegri, Alessandra Gregori, Claudio Monaci, Sara Gabriele, Tiziano Bernardo, Valentina Lorenzi, Claudia Romeo, Federico Scali, Lucia Scuri, Giorgio Bontempi, Maria Nobile, Luigi Bertocchi, Giovanni Loris Alborali, Adriana Ianieri, Sergio Ghidini

“…Locomotor disorders were the leading reason (70%), particularly trauma and fractures, followed by recumbency (13%) and calving-related issues (10%). Post-mortem findings showed limbs and joints as the most frequent condemnation sites (36%), often linked to trauma. …”
Get full text

Patient Perceptions of Social Media Use by Orthopaedic Surgeons: Comparing Responses Across the United States by Benjamin R. Wharton, M.D., Daniel J. Stokes, M.D., Alexander J. Johnson, M.D., Nicholas G. Girardi, B.S., Miranda G. Manfre, B.S., Carson Keeter, M.S., Kevin K. Shinsako, P.A.-C., Eric C. McCarty, M.D., Jonathan T. Bravman, M.D., Rachel M. Frank, M.D.

“…Purpose: To analyze patient perceptions of social media (SM) content posted by orthopaedic surgeons across the United States. …”
Get full text

Genome mining identifies a diversity of natural product biosynthetic capacity in human respiratory Corynebacterium strains by Ashley L. Cunningham, Ilya Y. Zhbannikov, Rachel Myers, Tommy H. Tran, Wei Gao, Katherine P. Lemon, Jhoanna N. Aquino, Jillian H. Hurst, Joon Won Yoon, Patrick C. Seed, Matthew S. Kelly

“…Among 161 genomes, we identified 672 BGCs, 495 of which were unique, including polyketide synthase, non-ribosomal peptide synthetase, ribosomally synthesized and post-translationally modified peptide, and siderophore families. …”
Get full text

Heightened Innate Immune and Inflammatory Gene Expression in the Skin of Severe COVID-19 Cases by Fernandes IG, Ferreira FM, De Sousa ESA, Pietrobon AJ, Teixeira FME, Ramos YÁL, Yendo TM, Pereira NZ, Sotto MN, Duarte-Neto AN, Orfali RL, Aoki V, Da Silva LFF, Duarte AJDS, Sato MN

“…In this study, we investigated the transcriptomic profile of skin samples from patients who succumbed to COVID-19.Patients and Methods: Skin autopsies from COVID-19 patients with post-mortem time of less than 20 hours were obtained from University of São Paulo Medical School Hospital and healthy skin samples, were submitted to RNA sequencing analysis. …”
Get full text