From Waste to Biocatalyst: Cocoa Bean Shells as Immobilization Support and Substrate Source in Lipase-Catalyzed Hydrolysis by Luciana Lordelo Nascimento, Bruna Louise de Moura Pita, César de Almeida Rodrigues, Paulo Natan Alves dos Santos, Yslaine Andrade de Almeida, Larissa da Silveira Ferreira, Maira Lima de Oliveira, Lorena Santos de Almeida, Cleide Maria Faria Soares, Fabio de Souza Dias, Alini Tinoco Fricks

“…Among them, the covalently immobilized enzyme on CBS (ORG-CB) showed the most balanced performance, achieving a catalytic efficiency (Ke) of 0.063 mM⁻¹·min⁻¹ (18.6% of the free enzyme), broad pH–temperature tolerance, and over 50% activity retention after eight reuse cycles. …”
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Molecular Assays on Cutaneous Swabs as an Effective, Non-Invasive Diagnostic Technique for Cutaneous Leishmaniasis: Results from a Retrospective Study Conducted in Italy by Anna Barbiero, Andrea Aiello, Nunziata Ciccone, Simona Pollini, Francesca Malentacchi, Maria Grazia Colao, Gian Maria Rossolini, Costanza Fiorelli, Daniela Massi, Alberto Antonelli, Sara Cuffari, Trentina Di Muccio, Alessandro Bartoloni, Michele Spinicci, Lorenzo Zammarchi

“…Background: The case confirmation of CL relies on the direct demonstration of the parasite in clinical specimens from skin tissues. Despite most research efforts focusing on biopsy samples as the preferred diagnostic specimen for the detection of <i>Leishmania</i> spp., the use of non-invasive sampling, such as cutaneous swabs, combined with the use of molecular assays, has shown promising results. …”
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Features of the simultaneous course of pulmonary aspergillosis and tuberculosis on the background of type 1 diabetes mellitus (clinical case) by O. M. Raznatovska, Yu. V. Myronchuk, O. S. Shalmin, A. V. Fedorets, O. A. Svitlytska

“…That’s why, a correct treatment of type 1 diabetes mellitus and timely preventive radiological examination of the thoracic cavity organs are especially important, as the diabetes mellitus is the most common premorbid background for TB and aspergillosis. …”
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Erythritol as a Next-Generation Sweetener: properties, metabolism, cardiovascular risk and gut health implications – A literature review by Julia Skowrońska-Borsuk, Martyna Narożniak, Adrianna Ewa Pękacka, Bartłomiej Czerwiec, Adam Borsuk, Joanna Katarzyna Pergoł, Malwina Wojtas, Julia Borkowska, Zuzanna Krupa, Julia Sposób

“…Due to its numerous drawbacks there is increasing demand for low-calorie sugar substitutes in human nutrition. Among the most frequently selected sugar substitutes is erythritol, a four-carbon polyol that is industrially produced through the fermentation of glucose by yeast. …”
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Molecular cloning and functional identification of an apple flagellin receptor MdFLS2 gene by Chen-hui QI, Xian-yan ZHAO, Han JIANG, Hai-tao LIU, Yong-xu WANG, Da-gang HU, Yu-jin HAO

“…Quantitative real-time PCR analysis showed that MdFLS2 was widely expressed in the different tissues of the apple and most highly expressed in the leaves. Furthermore, MdFLS2 was significantly induced by the flagellin elicitor peptide flg22. …”
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A model workflow for microfluidic enrichment and genetic analysis of circulating melanoma cells by Cristina Poggiana, Alessandro Francesco Piazza, Cristina Catoni, Ilaria Gallingani, Luisa Piccin, Stefania Pellegrini, Vittorio Aneloni, Valentina Salizzato, Jacopo Pigozzo, Alessio Fabozzi, Antonella Facchinetti, Chiara Menin, Paolo del Fiore, Simone Mocellin, Vanna Chiarion-Sileni, Antonio Rosato, Maria Chiara Scaini

“…Moreover, double enrichment with both CellSearch and Parsortix succeeded in removing most of the leukocyte contamination, resulting in an almost pure CMC sample suitable for genetic analysis. …”
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The quality of life in anemic patients with chronic heart failure, depending on the presence or absence of chronic kidney disease by N. G. Ryndinа, P. G Kravchun, O. M. Shelest

“…Chronic heart failure is one of the most common and prognostically adverse disease of cardiovascular system. …”
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Mapping grey matter and network abnormalities in seizure-onset patterns via stereotactic EEG by Tao Feng, Yanfeng Yang, Yihe Wang, Xiaotong Fan, Tianren Wang, Sichang Chen, Penghu Wei, Yongzhi Shan, Guoguang Zhao

“…Background: Mesial temporal lobe epilepsy (MTLE) is the most common medically refractory focal epilepsy, classified into seizure-onset patterns (SOPs) such as hypersynchronous (HYP) and low-voltage fast (LVF) rhythms. …”
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Effects of community action on animal vaccination uptake, antimicrobial usage, and farmers’ wellbeing in Ghana: study protocol for a cluster-randomized controlled trial by Francis Sena Nuvey, Günther Fink, Jan Hattendorf, Daniel T. Haydon, Gilbert Fokou, Kennedy Kwasi Addo, Jakob Zinsstag, Clemence Esse-Dibby, Bassirou Bonfoh

“…Recent studies identified Contagious Bovine Pleuropneumonia (CBPP) and Peste des Petits Ruminants (PPR) as the diseases most negatively affecting ruminant livestock productivity and farmers’ wellbeing in Ghana. …”
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Toward a Smartphone-Based and Conversational Agent–Delivered Just-in-Time Adaptive Holistic Lifestyle Intervention for Older Adults Affected by Cognitive Decline: Two-Week Proof-of... by Esther Brill, Rasita Vinay, Marcia Nißen, Priyam Joshi, Stefan Klöppel, Tobias Kowatsch

“… Abstract BackgroundDementia is projected to impact 152 million people by 2050, making it one of the most pressing global health challenges. The neurodegenerative process initiates well before clinical symptoms manifest, advancing from subjective cognitive decline (SCD) to mild cognitive impairment (MCI) and ultimately to dementia. …”
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Comparative performance and health economic analysis of prenatal screening for down syndrome in Fujian province, China by Lin Zheng, Nuolan Yin, Meiying Wang, Hailong Huang, Shuyu Zhang, Linjuan Su, Liangpu Xu

“…Cost-effectiveness analysis of the four strategies indicated that strategy 2 was the most cost-effective, with a cost-effectiveness ratio (CER) of 1,252,400 CNY and an incremental cost-effectiveness ratio (ICER) of -3,626,700 CNY, achieving improved screening outcomes while reducing costs. …”
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AI-assisted 3D versus conventional 2D preoperative planning in total hip arthroplasty for Crowe type II–IV high hip dislocation: a two-year retrospective study by Zhenbao Lu, Cuihua Yuan, Qingshan Xu, Yuhua Feng, Qiujin Xia, Xiaolu Wang, Jianfu Zhu, Jinqing Wu, Tihui Wang, Jiliang Chen, Xu Wang, Qijin Wang

“…Considering the higher cost, radiation exposure, and workflow complexity, its broader application warrants further investigation, particularly in identifying patients who may benefit most.…”
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A Turn-On Fluorescence Sensor Based on Guest-Induced Luminescence Ru(bpy)₃²⁺@UiO-66 for the Detection of Organophosphorus Pesticides by Jun Li, Jianlan Deng, Qian Tao, Chenyu Yan, Yuxuan Liu, Jianxiao Yang, Zhong Cao

“…Luminescent metal–organic frameworks (MOFs) are used for the detection of organophosphorus pesticides (OPs) due to their large surface area and pore volume as well as their special optical properties. However, most self-luminescent MOFs are not only complex to synthesize and unstable in water but also feature a “turn-off” sensing system, which has highly restricted their practical applications in OP detection. …”
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The Microbiological Characteristics and Genomic Surveillance of Carbapenem-Resistant <i>Klebsiella pneumoniae</i> Isolated from Clinical Samples by Mehwish Rizvi, Noman Khan, Ambreen Fatima, Rabia Bushra, Ale Zehra, Farah Saeed, Khitab Gul

“…Urine (29.7%) and blood (28.3%) were the most common sources, with infections predominantly affecting males (64.7%) and individuals aged 50–70 years. …”
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Erosión en la bahía de Samborombón y cambios en la dirección de los vientos, provincia de Buenos Aires, Argentina Erosion at the Samborombón Bay and changes in wind direction, prov... by Jorge O Codignotto, Walter C Dragani, Paula B Martin, María I Campos, Guadalupe Alonso, Claudia G Simionato, Rubén A Medina

“…A mild wind wave height increase has been detected at the Río de la Plata and the adjacent continental shelf, from 1970 to the present, as a consequence of the aforementioned climate variability. The most noticeable increase was observed between the 80&#8217;and 90&#8217;decades, in good agreement with a general increment of erosion at Samborombón Bay and neighboring areas. …”
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Patient and Provider Perspectives of a Web-Based Intervention to Support Symptom Management After Radioactive Iodine Treatment for Differentiated Thyroid Cancer: Qualitative Study by Alaina L Carr, Angela M Jenkins, Jacqueline Jonklaas, Kate Gabriel, Kristen E Miller, Kristi D Graves

“…Focus group participants identified the most prevalent RAI symptoms that were difficult to manage as: dry mouth (11/22, 50%), salivary gland swelling (8/22, 36%), and changes in taste (12/22, 55%). …”
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Sodium houttuyfonate inhibits the cariogenic virulence of Streptococcus mutans through the downregulation of VicRK two components pathway by Yaqi Chi, Ye Wang, Di Fu, Lin Yao, Mingying Wei, Ge Zhou, Guang Yang, Ling Zou, Biao Ren

“…Background Caries is one of the most common diseases worldwide, and Streptococcus mutans is considered to be the primary cariogenic pathogen of dental caries. …”
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Gliosarcoma: A Very Rare Biphasic Histopathological Engima Associated with Very Poor Prognostic Parameters by Pal Ruchismita, CD Anand, J Sowmiya, TP Jayaselvan, Manasa Raj

“…These tumours generally present as well-defined masses and are most frequently observed in adults between the ages of 40 and 60 years, with a slight predominance in males. …”
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Depressed mood affects the process of biological aging, analyses from the NHANES dataset by Yuan Tian, Yuan Tian, Qiao Lu, Qiao Lu, Jing Li, Jing Li, Xiaobo Zhou, Xiaobo Zhou, Luyao Wang, Luyao Wang, Xuemei Zhong, Xuemei Zhong, Yiping Luo, Yiping Luo

“…Notably, overeating and low self-perception emerged as the most significant contributors to the scores on the Patient Health Questionnaire-9 (PHQ-9) scale.ConclusionDepressive symptoms are linked to accelerated biological aging. …”
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Effect of Foliar Application of Calcium and Salicylic Acid on Fruit Quality and Antioxidant Capacity of Sweet Pepper (<i>Capsicum annuum</i> L.) in Hydroponic Cultivation by Anna Sobczak-Samburska, Ewelina Pióro-Jabrucka, Jarosław L. Przybył, Leszek Sieczko, Stanisław Kalisz, Janina Gajc-Wolska, Katarzyna Kowalczyk

“…Fruits of both varieties showed high concentrations of health-promoting constituents, including potassium, phosphorus, vitamin C (over 47 mg 100 g⁻¹ of FW (fresh weight)), and carotenoids, with capsanthin being the most abundant (more than 1226 μg 100 g⁻¹ of FW). …”
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