Multi-omics investigation reveals unique markers in Klebsiella pneumoniae compared to closely related species by Lena-Sophie Swiatek, Kristin Surmann, Elias Eger, Justus U. Müller, Manuela Gesell Salazar, Stefan E. Heiden, Guido Werner, Nils-Olaf Hübner, Jürgen A. Bohnert, Karsten Becker, Uwe Völker, Michael Schwabe, Katharina Schaufler, Katharina Schaufler

“…Among these, KP is by far the most clinically significant, with the emergence of multidrug-resistant and hypervirulent strains leading to severe infections and limited treatment options, underscoring the need to understand the genomic features of KP.MethodsThis study compared globally disseminated KP lineages with less abundant KV strains in synthetic human urine (SHU) across multiple omics levels to identify characteristics differentiating these closely related species. …”
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Augmented expression of RUNX1 deregulates the global gene expression of U87 glioblastoma multiforme cells and inhibits tumor growth in mice by Yoel Bogoch, Gilgi Friedlander-Malik, Lior Lupu, Ekaterina Bondar, Nitzan Zohar, Sheila Langier, Zvi Ram, Ido Nachmany, Joseph M Klausner, Niv Pencovich

“…Glioblastoma multiforme is the most common and aggressive primary brain tumor in adults. …”
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Driplines Layout Designs Comparison of Moisture Distribution in Clayey Soils, Using Soil Analysis, Calibrated Time Domain Reflectometry Sensors, and Precision Agriculture Geostatis... by Agathos Filintas

“…The modeling results revealed that the best-fitted geostatistical model for soil apparent dielectric <i>Ka</i> was the Gaussian model, while spherical and exponential models were identified to be the most appropriate for kriging modelling, and spatial and temporal imaging was used for accurate profile SWC <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><msub><mrow><mi>θ</mi><mi>v</mi></mrow><mrow><mi>T</mi><mi>D</mi><mi>R</mi></mrow></msub><mo> </mo></mrow></semantics></math></inline-formula>(m³·m⁻³) M1 and M2 maps using TDR sensors. …”
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First metagenome- and metatranscriptome dataset of Thecaphora frezzii teliospores, assembly and annotation of a new bacterial genome by Renee S. Arias, John T. Dobbs, Valerie A. Orner, E. Cinthia Conforto, Alejandro M. Rago, Luis I. Cazon, Victor S. Sobolev, Imana L. Power, Marshall C. Lamb, Alicia N. Massa

“…De novo assembly of PacBio reads revealed the whole genome of a Luteibacter sp. with 88.56% or lower similarity to those in NCBI database, whereas most RNA sequences (> 10 million reads) mapped to the new Luteibacter sp. genome assembled here. …”
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Exosomal miR-93-3p targets EIF4EBP1 to regulate macrophage polarization and accelerate wound healing post-anal fistula surgery by Wei Zhang, Wenzhe Feng, Junhong Chen, Ruoxi Cao, Xi Chen

“…The direct interplay between miRNA and its target gene was verified through a dual-luciferase reporter (DLR) assay.ResultsMicroarray analysis and qPCR validation identified miR-93-3p as the most significantly DEMs. miR-93-3p overexpression markedly downregulated M1 macrophage marker CD86 and pro-inflammation cytokines IL-1β, IL-6, and TNF-α, while upregulating M2 markers Arg-1, CD206, and anti-inflammation cytokines IL-10 and TGF-β in functional assays. …”
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The Effects of Dual Antiplatelet Therapy on the Occurrence of Recurrent Cardiovascular Events After Myocardial Infarction in Obese Patients by Borovyk K., Rindina N., Kravchun P., Tabachenko O., Yermak O., Romanyuk M.

“…Consequently, in the group of patients whose treatment included ASA with ticagrelor in DAPT, re­occurrence of coronary events was observed fewer than in the group of patients receiving ASA with clopidogrel (p < 0.05). Conclusions. Most patients (69.30 %) with obesity after myocardial infarction were included in the high­risk group of six­month lethality in terms of the number of GRACE scale points. …”
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A spatial clustering-based approach to design monitoring networks of infectious diseases: a case study of hand, foot, and mouth disease by Shuting Li, Yuanhua Liu, Ke Li, Zengliang Wang, Michael P. Ward, Wei Tu, Jiayao Xu, Rui Yuan, Lele Zhang, Na Wang, Jidan Zhang, Yu Zhao, Henry S. Lynn, Zhaorui Chang, Zhijie Zhang

“…Among the four methods compared, SCSS exhibited the lowest and most centralized RMSE. Conclusions SCSS proved to be more accurate and stable than traditional methods, which overlook spatial information. …”
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Groundwater–CO₂ emissions relationship in Dutch peatlands derived by machine learning using airborne and ground-based eddy covariance data by L. M. van der Poel, L. V. Bataille, B. Kruijt, W. Franssen, W. Jans, J. Biermann, A. Rietman, A. J. V. Buzacott, Y. van der Velde, R. Boelens, R. W. A. Hutjes

“…It is generally accepted that mitigation measures should include raising the water level, and the exact influence of water table depth has been increasingly studied in recent years. Most studies do this by comparing annual eddy covariance (EC) site-specific CO<span class="inline-formula">₂</span> budgets to mean annual effective water table depths. …”
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Ammonia emissions from beef cattle feedyards: a review by Myeongseong Lee, Myeongseong Lee, Brent W. Auvermann, Brent W. Auvermann, Luis O. Tedeschi, Jacek A. Koziel, Carolina B. Brandani, Carolina B. Brandani, Vinícius N. Gouvêa, Vinícius N. Gouvêa, Jason K. Smith, Jason K. Smith, Kenneth D. Casey, Kenneth D. Casey

“…Of those, precision diet feeding to meet but not exceed protein requirements appeared to be the most practical way to reduce ammonia emissions over the animals’ feeding period; laboratory studies suggested that shorter-term reductions in emission flux may be possible with the other approaches, but they were far more speculative at this point as to both their efficacy and their cost of implementation.…”
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MAintAin Activity in Arthritis: A study protocol of the co-development and feasibility testing of a physical activity maintenance intervention [version 2; peer review: 2 approved] by David French, Deirdre Hurley Osing, Aisling Walsh, Suzanne McDonough, Aoife Stephenson, Kathleen Bennett, Noreena Coyle, Chloe Grimmett, Claire Kinneavy, Stacey Grealis, Helen French, Oliver Fitzgerald, Mick Thacker, Maria Stokes

“…Whilst various programmes support people with arthritis to become active, most individuals reduce their level of PA after completion of a structured exercise programme. …”
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Expression heterogeneity of ABC-transporter family genes and chemosensitivity genes in gastric tumor, carcinomatosis and lymph node metastases by M. M. Tsyganov, I. A. Tsydenova, V. A. Markovich, M. K. Ibragimova, E. O. Rodionov, S. A. Tuzikov, N. V. Litvyakov

“…For ABC transporter genes, ABCG1 (3.1 ± 1.1; max 32.0), ABCG2 (7.9 ± 2.3; max 54.1), ABCG2 (9.6 ± 3.8; max 101.0) were the most expressed genes in gastric tumor tissue, carcinomatosis and lymph node metastasis, respectively. …”
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Assessing ChatGPT’s Educational Potential in Lung Cancer Radiotherapy From Clinician and Patient Perspectives: Content Quality and Readability Analysis by Cedric Richlitzki, Sina Mansoorian, Lukas Käsmann, Mircea Gabriel Stoleriu, Julia Kovacs, Wulf Sienel, Diego Kauffmann-Guerrero, Thomas Duell, Nina Sophie Schmidt-Hegemann, Claus Belka, Stefanie Corradini, Chukwuka Eze

“…ResultsReadability analysis classified most responses as “very difficult to read” (university level) or “difficult to read” (upper secondary school), likely due to the use of medical language and long sentence structures. …”
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Prevalence and factors associated with the use of long-acting reversible and permanent contraceptive methods among women who desire no more children in Bangladesh by Swapna Akter, Md. Mehedi Hasan Khan, Abu Hasanat Mohammad Kishowar Hossain, M. Sheikh Giash Uddin, Md. Aminul Haque

“…Abstract Background Long-acting reversible and permanent contraceptive methods (LARC/PM) with high efficacy and continuity of use are highly effective pregnancy prevention methods. However, most sexually active women do not use it and end up with unintended pregnancies and unsafe abortions in Bangladesh. …”
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Malignant Bowel Obstruction in Ovarian Cancer Recurrence: The Role of Palliative Surgery in a Decision-Making Process by Eugenia De Crescenzo, Marianna Fontana, Giulia Dondi, Stefano Friso, Alessandro Bovicelli, Marco Di Stanislao, Marco Tesei, Alessandra De Palma, Pasquale Chieco, Matteo Rottoli, Paolo Bernante, Gloria Ravegnini, Anna Myriam Perrone, Pierandrea De Iaco

“…Of the 9 patients treated surgically, 2 (22%) were given surgery as a first choice. In most cases (78%), surgery was decided upon when medical treatment failed. …”
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Serum C‐Terminal Agrin Fragment With Acute and Long‐Term Exercise and Angiotensin II Type I Receptor Blockade by Casper Soendenbroe, Mette F. Heisterberg, Jesper L. Andersen, Michael Kjaer, Abigail L. Mackey

“…C‐terminal agrin fragment (CAF) is the most commonly used blood‐based biomarker of neuromuscular junction degradation in aging, inactivity and disease, but large unexplained interindividual variation exists, limiting its diagnostic and prognostic value. …”
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Interaction between the TCF7L2 gene and dietary intake on metabolic syndrome risk factors among Saudi Arabian adults by Maha S. Al-odinan, Najlaa M. Aljefree, Noha M. Almoraie, Marwan A. Bakarman, Hani A. Alhadrami, Hani A. Alhadrami, Hani A. Alhadrami, Israa M. Shatwan

“…IntroductionTranscription factor-7-like 2 (TCF7L2) is the most critical type 2 diabetes (T2D) gene identified to date. …”
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Pooling data for primary total knee implants across national registries: is the same implant used in multiple registries and for the same patient group? An observational study by Lotje Hoogervorst, Rob G H H Nelissen, Liza van Steenbergen, Alma B Pedersen, Eskild Bendix Kristiansen, Martin Lindberg-Larsen, Marina Torre, Enrico Ciminello, Riccardo Valentini, Alexander W Grimberg, Yinan Wu, Perla J Marang-van de Mheen

“…Statistically and clinically significant differences in age for the same TK implant across registries were observed for 29 TK implants (62%) and 3 TK implants (6%), respectively; for other characteristics these were for BMI 30 (64%) and 0 (0%) TK implants; for male proportion 23 (49%) and 17 (36%) TK implants; and for diagnosis of osteoarthritis 42 (89%) and 34 (72%) TK implants, respectively. Conclusion: Most specific TK implants and TK procedures were used across multiple registries, but they were often used in patients with different characteristics. …”
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Subretinal suspensions of hiPSC-derived retinal pigment epithelium cells form functional monolayers in NOD-SCID mice facilitating treatment of advanced retinal diseases by Xiaojing Song, Guanjie Gao, Ke Ye, Ping Xu, Yuan Wang, Suai Zhang, Dandan Zheng, Jian Ge, Xiufeng Zhong

“…Introduction: Transplantation of human induced pluripotent stem cells derived retinal pigment epithelium (hiPSC-RPE) is regarded as one of the most promising strategies for advanced retinal degenerative diseases leading to blindness, such as age-related macular degeneration. …”
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Does less invasive surfactant administration during high-flow nasal cannula oxygen treatment reduce the need for invasive ventilation in late preterm and early term born infants wi... by Anne Greenough, Theodore Dassios, Eleanor Jeffreys, Chris Harris, Allan Jenkinson, Ravindra Bhat, Ourania Kaltsogianni

“…Introduction Up to 40% of late preterm and early term infants require neonatal admission with respiratory disease being the most common cause. Less invasive surfactant administration (LISA) has previously been used with good success in the very preterm population, but it is unclear whether late preterm infants suffering from respiratory distress syndrome and early term infants suffering from secondary surfactant deficiency might also benefit from LISA. …”
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Survey on the awareness, use of and attitude of trying heated tobacco products in middle school and college students in Guangzhou city: a cross-sectional survey by Kun XIAO, Cuiling ZHANG, Cuichan ZHONG, Yueling JIN, Lanfang LIANG, Yongqi WANG, Zhenyu LIANG, Pusheng XU

“…Regarding attitudes toward trying HTPs, 3.41% were "very interested" and 4.72% were "somewhat interested." The most common reasons for trying HTPs were "less harmful to health" (24.67%), "simply like it, no particular reason" (24.23%), "curiosity" (13.36%), and "can reduce smoking" (10.28%). …”
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