Cas9-expressing cattle using the PiggyBac transposon all-in-one system by Dong-Hyeok Kwon, Gyeong-Min Gim, Soo-Young Yum, Kyeong-Hyeon Eom, Song-Jeon Lee, Sang-Eun Han, Hee-Soo Kim, Hyeong-Jong Kim, Woo-Sung Lee, Woo-Jae Choi, Ji-Hyun Lee, Do-Yoon Kim, Dae-Jin Jung, Dae-Hyun Kim, Jun-Koo Yi, Byeong-Ho Moon, Won-You Lee, Goo Jang

“…However, genetic engineering in cattle has lagged due to long gestation periods, single embryo pregnancies, and high rearing costs. Additionally, the slow validation of germline transmission and the absence of germline-competent embryonic stem cells hinder progress. …”
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The effect of a systematic multi-dimensional assessment in severe uncontrolled asthma: a literature review and protocol for an investigator-initiated, open-label, randomized-contro... by L. Bult, G. J. Braunstahl, J. G. J. V. Aerts, D. Bänffer, J. S. J. A. van Campen, M. S. van Daalen, Y. van Dooren, U. Flanders, E. S. Geurts, P. P. Hekking, R. Heller - Baan, M. J. A. Jans, J. H. Kappen, R. C. A. Mies, B. Oppedijk, M. de la Roij - Hartmans, S. Van der Sar - Van der Brugge, Y. Türk, E. Vis, R. Wolters, E. C. Vasbinder, J. C. C. M. in ‘t Veen

“…These patients are eligible for treatment with biologicals, which provide significant benefits but are costly and need precise indication. However, identifying all individual treatable traits before diagnosing severe asthma is challenging. …”
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Chlorhexidine-alcohol versus Povidone-iodine plus Alcohol as Preoperative Antiseptic for Prevention of Surgical Site Infection in Caesarean Section: A Randomised Controlled Trial by Shravanthi Swamy, Aruna M Biradar, Ekta Chhabra, Shailaja Rajendra Bidri, Neelamma Patil, Shreedevi Kori, Shilpa Lakshmi, Preethi Malapure

“…Surgical Site Infection (SSI) remains a significant complication, impacting maternal recovery and healthcare costs. Aim: To evaluate the efficacy of chlorhexidine-alcohol versus Povidone-Iodine (PI) plus alcohol in preventing SSI in patients undergoing CS. …”
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Virtual Intervention for Vertebral frActures (VIVA): protocol for a feasibility study of a multicentre randomized controlled trial by Ada Sevinc, Alexandra Papaioannou, Suzanne N. Morin, Jennifer A. Watt, Raheem B. Kherani, Sheila Brien, Larry Funnell, Lehana Thabane, Lauren A. Beaupre, Jenna C. Gibbs, Heather Keller, Caitlin McArthur, Matteo Ponzano, Sonia Singh, Sharon Straus, Jenny Thain, Zachary J. Weston, Timothy H. Wideman, Christopher D. Witiw, Lora Giangregorio

“…The secondary outcomes include costs relative to benefit, effects on health-related outcomes (e.g. 30-s chair stand, Brief Pain Inventory, Quality of Life Questionnaire of the European Foundation for Osteoporosis-41, SCREEN-14), and outcomes related to implementation (e.g. participant and provider experience using semi-structured interviews, fidelity, number of screened and enrolled participants by referral source, fracture verification process). …”
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Improving the accuracy of Respiratory Syncytial Virus (RSV) incidence estimates among hospitalised adults in Bristol, UK by Katie Lihou, Robert Challen, Anastasia Chatzilena, George Qian, Glenda Oben, Jade King, Serena McGuinness, Begonia Morales-Aza, Kaltun Duale, Ainhoa Rodriguez Pereira, William Healy, Jennifer Oliver, Nick Maskell, Adam Finn, Leon Danon, Catherine Hyams, Avon C. A. P. Research Group

“…The implementation of effective RSV vaccines could reduce morbidity, mortality and associated costs of disease. Adult RSV burden accuracy is improved by adjustment for test characteristics due to the impact of imperfect specificity when positivity-rates are low, and this is particularly important for out-of-season estimates. …”
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Comparison of treatment patterns, efficacy and safety between generic and branded atorvastatin users in China: a multicenter, retrospective propensity score-matched cohort study by Xiaoxuan Xing, Zhizhou Wang, Ke Wang, Yiming Hua, Xiaoxi Li, Kejia Le, Wenbing Ma, Yingyun Guan, Aiping Deng, Xiong Yun, Hongfu Cai, Yongning Lyu, Guoying Xiong, Min Yang, Siyang Wang, Chaojun Xue, Jing Zhang, Qiushi Guo, Song Hu, Jing Li, Xianzhe Dong, Lan Zhang

“…Conclusion Generic atorvastatin had higher adherence and persistence and lower costs vs the branded drug, with no significant differences in efficacy and safety. …”
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Protocol of a randomized, single-blind, controlled trial of an 18-month, home-based, high-intensity, exercise intervention in older, cognitively unimpaired APOE ε4 carriers by Stephen M. Rao, Alexandria L. Chorba, M. J. Helppi, Alina Tuladhar, Amanda L. Penko, Sarah Holley, Brandon Nehls, Sabrina Paulino, Matthew C. Streicher, Audrey Zhu, Kellie Bruening, Sally Durgerian, Katherine Koenig, Mark Lowe, Wanyong Shin, Stephen E. Jones, Rachel Galioto, Jagan A. Pillai, Lynn M. Bekris, James B. Leverenz, Anson B. Rosenfeldt, Tamanna Singh, Gerald J. Beck, J. Carson Smith, Jay Alberts, Jay Alberts

“…Secondary outcomes include comprehensive neurocognitive and physical function test batteries, MRI scans including structural and functional connectivity measures, and blood-based biomarkers.HypothesesOver an 18-month interval, physically inactive ε4 carriers who engage in high-intensity aerobic exercise will demonstrate less cognitive decline and hippocampal atrophy than physically inactive ε4 carriers who did not participate in a formal exercise program.ConclusionSuccessful demonstration of a scalable, home-based, high-intensity aerobic exercise intervention in altering the trajectory of AD pathophysiology and its effects on cognitive functioning will transform AD treatment, improve patient outcomes and quality of life, and reduce healthcare costs.…”
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T cell-specific non-viral DNA delivery and in vivo CAR-T generation using targeted lipid nanoparticles by Jacek Lubelski, Eline van Diest, Ator Ashoti, Christina Zotou, Hanneke Rijssemus, Raymond Schiffelers, Zhiyong Lei, Maurits Geerlings, Jaime Fernández Bimbo, Daniel E Murphy, Martijn J W Evers, Suneel A Narayanavari, Diana Pereira Vaz, Nadine Saber, Peter Mayrhofer

“…However, treatment accessibility is hindered by high costs, long manufacturing times, and the need for specialized centers and inpatient care. …”
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Assessment of bone mineral density in epileptic patients with long-term antiepileptic therapy: pilot data by N. A. Sivakova, I. V. Abramova, V. P. Rybasova, O. D. Bolshunova, E. D. Kasyanov, G. V. Rukavishnikov, M. A. Khobeysh, M. Yu. Sorokin, L. V. Lukina, N. I. Ananyeva, R. F. Nasyrova, V. A. Mikhailov, G. E. Mazo

“…The problem of decreased bone mineral density and frequent fractures in patients with epilepsy is an important and understudied issue that significantly reduces quality of life and involves significant economic costs for the treatment and rehabilitation of epileptic patients. …”
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Delivering a national de-adoption programme: a documentary analysis of local commissioning policy compliance with England's Evidence-based Interventions programme (EBI) by Carmel Conefrey, Nicola Farrar, Maeve Coyle, Mike Bell, Jane Blazeby, Christopher Burton, Jenny Donovan, Andy Gibson, Joel Glynn, Tim Jones, Angus McNair, Josie Morley, Amanda Owen-Smith, Ellen Rule, Gail Thornton, Victoria Tucker, Iestyn Williams, William Hollingworth, Leila Rooshenas

“…Abstract Background In 2019 the English National Health Service (NHS) launched a national de-adoption programme to stop or limit access to surgical procedures considered to have little, or uncertain, evidence of benefit to justify their risks and/or costs: the Evidence-Based Interventions (EBI) programme. …”
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Understanding the interests of academics from diverse disciplines to identify the prospective focus for a UK-based transdisciplinary network involving farm-to-fork stakeholders on... by K. Marie McIntyre, Maha Khan, Martha Betson, Lucy Brunton, Hernan Botero Degiovanni, Andrew P. Desbois, Mahmoud Eltholth, Paul Hurley, Lisa Morgans, John E. Pearl, Ruben Sakrabani, Orla Shortall, Katharina Watson, Jennifer Cole

“…This co-creation platform for network-wide systems-based activities would reduce inefficiencies in AMR-related activities around agrifood, providing a cross-cutting, cohesive community to deliver transformational guidance on relevant, practical agrifood solutions that add value by reducing AMR, antimicrobial usage and associated costs, and decreasing resultant environmental contamination by prioritising challenges, sharing knowledge and best practice, and co-creating practical solutions with key stakeholders. …”
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Knowledge and Practice Regarding Pressure Injury Prevention among Nurses at King Abdul-Aziz Specialist Hospital by Afnan Sahrah, Wafaa Althobaiti, Sahar Albalawi, Ensherah Althobiti, Turki Alfattah, Abdulellah Almalki, Ahlam Alotaibi, Maha Alkhaldi, Talal Alqurashi, Faten Sahrah, Munirah Alfifi, Rami Alnemari, Alaa Alzahrani

“…These skin lesions cause discomfort and elevate the risk of severe infections, leading to increased healthcare costs and a high mortality rate due to sepsis. Thus, prevention requires an interdisciplinary approach to care. …”
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Cucurbitacin B inhibits HIF-1α and attenuates non-small cell lung cancer via ZFP91 by Lei Song, Lei Song, Jing Han, Run Wang, Shen Cao, Yi Tai, Xinyu Wang, Yanjin Zheng, Shufeng Jin, Yiqian Shen, Cai Qin Liu, Yue Xing, Hong Xiang Zuo, Ming Yue Li, Juan Ma, Xuejun Jin

“…Although some progress has been made in the treatment of NSCLC, drug resistance, high costs, and significant individual variability continue to be major obstacles to effective treatment. …”
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Clinical predictors of flare and drug-free remission in rheumatoid arthritis: preliminary results from the prospective BIO-FLARE experimental medicine study by Iain B McInnes, Stefan Siebert, Arthur Pratt, Andrew Filer, Karim Raza, Wan-Fai Ng, Christopher D Buckley, Dawn Teare, Andrew Melville, Amy Anderson, Andrew McGucken, Bernard Dyke, Catharien M U Hilkens, John Isaacs, Kenneth F Baker, Mohadeseh Shojaei Shahrokhabadi, Shaun Hiu, Theophile Bigirumurame, Carl Goodyear, Fiona Rayner, Sean Kerrigan, Jonathan Prichard

“…However, long-term treatments can carry side effects and associated financial costs. In addition, some patients still experience painful and debilitating disease flares, the mechanisms of which are poorly understood. …”
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Barriers and enablers of HPV vaccination among caregivers of school-age girls in resource-limited regions of Western Sichuan, China: a mixed-methods study by Qiwen Zhang, Lu Zhang, Zubo Huang, Yue Zeng, Yueshan Wang, Jieru Peng, Yan Kou, Wei Huang, Gang Zhu, Wencheng Long, Yao Dong, Chunxia Yang

“…Ensuring vaccine supply, reducing costs, improving caregivers’ knowledge, and increasing vaccination accessibility could significantly enhance vaccination rates in resource-limited regions of western China.…”
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Intensive and critical care nurses’ compliance, barriers, and challenges with ventilator-associated pneumonia prevention: a cross-sectional study by Hazel Novela Villagracia, Tahani Nasser Alhejaili, Bushra Alshammari, Salwa Abd El Gawad Sallam, Awatif Mansour Alrasheeday, Lea L. Dando, Odeta A. Nacubuan, Habib Alrashedi, Shaimaa Mohamed Nageeb, Larry Terence O. Cornejo, Rico William A. Villagracia, Soha Kamel Mosbah Mahmoud, Ingrid Jacinto-Caspillo, Rizal Angelo N. Grande, Daniel Joseph E. Berdida, Bahia Galal Abd Elrazik Siam

“…Abstract Background Ventilator-associated pneumonia (VAP) poses a significant challenge in critical care settings, impacting patient outcomes and healthcare costs. In Saudi Arabia, several studies have reported concerns regarding VAP, including variable VAP rates, inconsistent compliance with and knowledge of VAP prevention practices, and inadequate national data. …”
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Decentralized biobanking platform for organoid research networks by Marielle Gross, Marielle Gross, Ananya Dewan, Mario Macis, Mario Macis, Eve Budd, M. Eifler, Olayinka Odeniran, Jeff Kahn, Jeff Kahn, Jeff Kahn, Robert Clell Miller, Robert Clell Miller, William Sanchez

“…Implementing de-bi with ERC-1155 showed potential to minimize gas costs of on-chain activity vs ERC-721, though complementary layer-2 solutions will be essential for economic viability.ConclusionDecentralized biobanking has the potential to enhance efficiency, increase translational impact and drive research discovery through implementation of NFT digital twins for organoid research networks. …”
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A patient self-report disability identifier within health service electronic medical records: evaluation of patient, carer and clinician acceptability by Joanne Rowe, Alexandra Devine, Nicole Merrick, Marie Huska, Kristen Morris, Kath Feely, Ahuva Segal, Louise Mogg, Jenni Medland, Rebecca Jamwal, Alison Smith, Maricar Gleich, Jeremiah Munakabayo, Christine Imms

“…Data analysis explored perspectives and experiences across eight constructs: general acceptability, affective attitude, burden, ethicality, intervention coherence, opportunity costs, self-efficacy, and perceived effectiveness. …”
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Targeted next-generation sequencing: a promising approach for Mycobacterium tuberculosis detection and drug resistance when applied in paucibacillary clinical samples by Wenting Jin, Meixia Wang, Yang Wang, Beidi Zhu, Qingqing Wang, Chunmei Zhou, Pei Li, Chaohui Hu, Jun Liu, Jue Pan, Jiachang Chen, Bijie Hu

“…The tNGS assay used in this study costs USD 96 and has a 12 hour turnaround time. Nonetheless, tNGS has a great deal of promise for enhancing TB detection while also addressing DR strains.…”
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Surgeon Sex and Postoperative Resource Utilization: A Population-Based Cohort Study by Kiyan Heybati, BHSc, Raj Satkunasivam, MD, MS, Khatereh Aminoltejari, MD, MSc, FRCSC, Hannah S. Thomas, MBChB, MS, Arghavan Salles, MD, PhD, Natalie Coburn, MD, MPH, Frances C. Wright, MD, Med, FRCSC, Lesley Gotlib Conn, PhD, Amy N. Luckenbaugh, MD, Sanjana Ranganathan, MD, Carlos Riveros, MD, Jason Sutherland, PhD, Colin McCartney, MBChB, PhD, FRCA, FFARCSI, FRCPC, Kathleen Armstrong, MD, MSc, FRCSC, Barbara Bass, MD, Allan S. Detsky, MD, PhD, CM, Angela Jerath, MD, MSc, Christopher J. D. Wallis, MD, PhD

“…Prior studies have shown that patients of female physicians experience better outcomes and have lower healthcare costs compared with patients of male physicians. …”
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