Understanding the future of dengue in Malaysia: Assessing knowledge, attitude, and homeowner practices in mitigating climate-driven risks [version 1; peer review: 2 approved] by Sapna Shridhar Patil, Izzah Athirah Rosli, Fatin Norhasny Leman, Nur Hasnah Ma’amor, Nor Asiah Muhamad, Kulankara Balan Venugopalan, Prabal Bhargava, Mahalingam Dinesh, Weng Keong Yau, Chai Hong Yeong, Eng Hwa Wong, Arasarethinam Mugilarasi, Ameya Ashok Hasamnis, Imam Shaik, Chung Yeng Looi, Pei Pei Chong, Priya Madhavan, Lei Hum Wee, Anitha Ponnupillai, Jo Ann Andoy-Galvan, Yin How Wong

“…This is a cross-sectional study conducted among adult residents of low-cost housing apartments in an urban poor community in Kuala Lumpur, Malaysia involving approximately 16,000 residents. …”
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Reverse-sequence endoscopic versus conventional open nipple-sparing mastectomy with implant-based breast reconstruction: a single-center prospective cohort study (RECO study) by Tianyuan Li, Qing Zhang, Yanyan Xie, Huanzuo Yang, Kawun Chung, Hui Dai, Xiaoman Cao, Zhenggui Du

“…However, traditional minimal-access NSM is time-consuming, costly, and has a steep learning curve, hindering its widespread adoption. …”
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On the Upper Mid-Band 8 × 8 and 8 × 4 Device MIMO Performance Based on Applying Compact Backcover Four-Antenna Modules for 6G Smartphones With Increased Data Rates by Kin-Lu Wong, Wan-Ting Li, Chin-Lung Wang, Chun-Chi Wen, Wei-Yu Li

“…However, it is worth noting that the <inline-formula> <tex-math notation="LaTeX">$8\times 8$ </tex-math></inline-formula> MIMO requires twice the transmitted power of current fifth-generation (5G) <inline-formula> <tex-math notation="LaTeX">$4\times 4$ </tex-math></inline-formula> MIMO and has a much more complex system architecture. Thus, whether it is cost effective to apply the <inline-formula> <tex-math notation="LaTeX">$8\times 8$ </tex-math></inline-formula> MIMO for increased data rates is not clear. …”
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HIV self-testing alone or with additional interventions, including financial incentives, and linkage to care or prevention among male partners of antenatal care clinic attendees in... by Augustine T Choko, Elizabeth L Corbett, Nigel Stallard, Hendramoorthy Maheswaran, Aurelia Lepine, Cheryl C Johnson, Doreen Sakala, Thokozani Kalua, Moses Kumwenda, Richard Hayes, Katherine Fielding

“…No serious adverse events were reported. Costs per male partner who attended the clinic with a confirmed HIV test result were $23.73 and $28.08 for the HIVST + $3 and HIVST + $10 arms, respectively. …”
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Patient-derived colorectal microtumors predict response to anti-PD-1 therapy by Duy T. Nguyen, Matthew A. Schaller, Krista P. Terracina, Xia Xu, Diego I. Pedro, Alfonso Pepe, Juan M. Urueña, Zadia Dupee, Nickolas Diodati, Ryan A. Smolchek, Jack E. Famiglietti, Nhi Tran Yen Nguyen, Gerik W. Tushoski-Alemán, Kuoyuan Cheng, Lan Chen, Doug Linn, Vania Vidimar, Aquila Fatima, Soon Woo Kwon, Dongyu Sun, Hongmin Chen, Haiyan Xu, Brian Long, Lily Y. Moy, Bonnie J. Howell, George H. Addona, W. Gregory Sawyer

“…To improve efficiency, cost-effectiveness, and clinical outcomes, there is an essential need for a testing platform capable of promptly identifying evidence of anti-PD-1 response to inform treatment strategies. …”
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Decentralized Immunization Monitoring: Lessons Learnt from a Pilot Implementation in Kumbotso LGA, Kano State, Nigeria by Adam Attahiru, Yahaya Mohammed, Fiyidi Mikailu, Hyelshilni Waziri, Ndadilnasiya Endie Waziri, Mustapha Tukur, Bashir Sunusi, Mohammed Nasir Mahmoud, Nancy Vollmer, William Vargas, Yusuf Yusufari, Gustavo Corrêa, Heidi W. Reynolds, Teemar Fisseha, Talatu Buba Bello, Moreen Kamateeka, Adefisoye Oluwaseun Adewole, Musa Bello, Imam Wada Bello, Sulaiman Etamesor, Joseph J. Valadez, Patrick Nguku

“…Negative vaccine perceptions (50% delayed, 53.6% ZD) and distrust in health workers (46.4% delayed, 48.2% ZD) were significant barriers, though the caregiver intent to vaccinate was protective (OR: 0.27, <i>p</i> < 0.001). The cost of accessing immunization services appeared to have a minor effect on coverage, as the majority of caregivers of delayed and ZD children reported spending less than 200 Naira (equivalent to USD 0.15) on transport. …”
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Donor-derived CARCIK-CD19 cells engineered with Sleeping Beauty transposon in acute lymphoblastic leukemia relapsed after allogeneic transplantation by Federico Lussana, Chiara F. Magnani, Stefania Galimberti, Giuseppe Gritti, Giuseppe Gaipa, Daniela Belotti, Benedetta Cabiati, Sara Napolitano, Silvia Ferrari, Alex Moretti, Chiara Buracchi, Gian Maria Borleri, Benedetta Rambaldi, Giuliana Rizzuto, Anna Grassi, Muriel Paganessi, Cristian Meli, Sarah Tettamanti, Giulia Risca, Giulia Pais, Giulio Spinozzi, Fabrizio Benedicenti, Giovanni Cazzaniga, Chiara Capelli, Elisa Gotti, Martino Introna, Josée Golay, Eugenio Montini, Adriana Balduzzi, Maria Grazia Valsecchi, Giuseppe Dastoli, Alessandro Rambaldi, Andrea Biondi

“…Abstract Non-viral engineering can ease CAR-T cell production and reduce regulatory and cost requirements. We utilized Sleeping Beauty transposon to engineer donor-derived anti-CD19.CD28.OX40.CD3zeta T cells differentiated in cytokine-induced killer (CARCIK-CD19) for B-cell precursor acute lymphoblastic leukemia (BCP-ALL) patients relapsed after allogeneic hematopoietic stem cell transplantation (alloHSCT). …”
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A genomic duplication spanning multiple P450s contributes to insecticide resistance in the dengue mosquito Aedes aegypti by Bacot, Tiphaine, Haberkorn, Chloe, Guilliet, Joseph, Cattel, Julien, Kefi, Mary, Nadalin, Louis, Filee, Jonathan, Boyer, Frederic, Gaude, Thierry, Laporte, Frederic, Tutagata, Jordan, Vontas, John, Dusfour, Isabelle, Bonneville, Jean-Marc, David, Jean-Philippe

“…Experimental evolution suggested that this P450 duplication is associated with a significant fitness cost, potentially affecting its adaptive value in presence of other resistance alleles. …”
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Clinical evaluation of two pathogen enrichment approaches for next-generation sequencing in the diagnosis of lower respiratory tract infections by Xiao Lei, Xiumei Xu, Chao Liu, Lipeng Zhong, Shupeng Yin, Biaoxian Li, Ling Cao, Zhiting Xie, Jing Li, Xuan Zhang, Yaping Guo, Liang Zhang, Haiyan Lin, Sufeng Zhang, Chengsheng Zhang, Tian Gong

“…Next-generation sequencing (NGS) has emerged as a promising platform for the diagnosis of infectious diseases, albeit with high costs and challenges in result interpretation. Here we evaluated two NGS-based pathogen detection assays for the etiological diagnosis of pneumonia in a prospective cohort of 257 patients. …”
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Indigenous Malolactic Starter Cultures as Innovative Tools to Modify the Sensory Profile of a Wine: An Oenological Challenge by Elizabeth Naiquen Flores, Gabriel Alejandro Rivas, Andrea Cecilia Guillade, Natalia Soledad Brizuela, Marina Edith Navarro, Emma Elizabeth Tymczyszyn, Lucrecia Delfederico, Carolina Fabiana Perez, Liliana Carmen Semorile, Danay Valdes La Hens, Barbara Mercedes Bravo-Ferrada

“…This study evaluated the oenological potential of three innovative native malolactic fermentation starters (MLFS) on a pilot scale, aiming to offer local wineries cost-effective MLFS with native LAB strains. Alcoholic fermentation of Malbec grapes was carried out using a commercial yeast starter, followed by a sequential inoculation of native malolactic starters formulated with (i) mesophilic <i>Lactiplantibacillus plantarum</i> UNQLp11 and <i>Oenococcus oeni</i> UNQOe73.2, both from the Province of Río Negro; (ii) psychrotrophic <i>Lentilactibacillus hilgardii</i> UNQLh1.1 and <i>Oenococcus oeni</i> UNQOe19, both from the Province of Río Negro; (iii) mesophilic <i>Lactiplantibacillus plantarum</i> UNQLp1001, from the Province of Buenos Aires; and (iv) a commercial malolactic started, which served as a control. …”
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Enteric-coated microencapsulation improves the therapeutic effect of cinnamon oil on artificially infected chicken with Escherichia coli by Huanling Xing, Fang Zhou, Jianhao Lin, Junfeng Ban, Junyi Huang, Xinghai Li, Hanjun He, Yanqun Li, Mei Bai, Xiangxiu Liang, Hong Wu

“…These findings suggest enteric microcapsule system for CEO significantly enhances CEO's therapeutic potential, offering a promising strategy for developing more efficient and cost-effective natural antibacterial treatments for colibacillosis.…”
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Bioinoculant production composed by Pseudomonas sp., Serratia sp., and Kosakonia sp., preliminary effect on Allium cepa L., growth at plot scale by Andrea Blanco Vargas, Lina Rodríguez Gacha, Natalia Sánchez Castro, Laura Herrera Carlosama, Raúl Poutou Piñales, Lucía Díaz Ariza, Viviana Gutiérrez Romero, Claudia Rivera Hoyos, Leydi Ardila Leal, Aura Pedroza Rodriguez

“…To meet P requirements in agricultural settings, a low-cost culture medium (MT11B) was designed in which a bioinoculant was produced consisting of three bacterial isolates capable of solubilizing P from phosphoric rock (PR). …”
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Semantic Reasoning Using Standard Attention-Based Models: An Application to Chronic Disease Literature by Yalbi Itzel Balderas-Martínez, José Armando Sánchez-Rojas, Arturo Téllez-Velázquez, Flavio Juárez Martínez, Raúl Cruz-Barbosa, Enrique Guzmán-Ramírez, Iván García-Pacheco, Ignacio Arroyo-Fernández

“…Large-language-model (LLM) APIs demonstrate impressive reasoning capabilities, but their size, cost, and closed weights limit the deployment of knowledge-aware AI within biomedical research groups. …”
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Efficiently constructing complete genomes with CycloneSEQ to fill gaps in bacterial draft assemblies by Hewei Liang, Yuanqiang Zou, Mengmeng Wang, Tongyuan Hu, Haoyu Wang, Wenxin He, Yanmei Ju, Ruijin Guo, Junyi Chen, Fei Guo, Tao Zeng, Yuliang Dong, Yuning Zhang, Bo Wang, Chuanyu Liu, Xin Jin, Wenwei Zhang, Xun Xu, Liang Xiao

“… Current microbial sequencing relies on short-read platforms like Illumina and DNBSEQ, which are cost-effective and accurate but often produce fragmented draft genomes. …”
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Liquid biopsy in gynecological cancers: a translational framework from molecular insights to precision oncology and clinical practice by Canio Martinelli, Alfredo Ercoli, Giuseppe Vizzielli, Sharon Raffaella Burk, Maria Cuomo, Vrunda Satasiya, Housem Kacem, Simone Braccia, Giulio Mazzarotti, Irene Miriello, Manuela Nana Tchamou, Stefano Restaino, Martina Arcieri, Alice Poli, Veronica Tius, Silvana Parisi, Stefano Pergolizzi, Giuseppe Iatì, Chiara Conti Nibali, Cristina Pizzimenti, Ludovica Pepe, Antonio Ieni, Salvatore Cortellino, Antonio Giordano

“…Although several FDA-approved assays and promising commercial tests illustrate the field’s rapid evolution, many translational hurdles remain, including the need for harmonized protocols, larger prospective clinical trials, and cost-effectiveness analyses. Crucially, our synthesis clarifies the pivotal role of interdisciplinary collaboration. …”
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Investigating the long-term public health and co-benefit impacts of an urban greenway intervention in the UK: a natural experiment evaluation – study protocol by Frank Kee, Ruoyu Wang, Ruth F Hunter, Mike Clarke, Sophie Jones, John Barry, Ciaran O’Neill, Leandro Garcia, Christopher R Cardwell, Hüseyin Küçükali, Claire Cleland, Aideen Maguire, Niamh O’Kane, Selin Akaraci, Christopher Tate, Shay Mullineaux, Geraint Ellis, Brendan Murtagh, Anna Jurek-Loughrey, Dominic Bryan, Jeremy Hilton, Mehdi Hafezi, Natalie Clewley

“…A range of economic evaluation methods, including a cost-effectiveness analysis and social return on investment (SROI), will be employed. …”
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Enhancing fermentation quality and fiber decomposition of Phragmites australis silage by introducing Bacillus subtilis and lactic acid bacteria consortia by Yong Liu, Songbai Zhang, Junrui Liao, Nazir Ahmad Khan, Shaoxun Tang, Chuanshe Zhou, Zhiliang Tan, Asmaa Elnagar, Ibrahim F. Rehan, Ibrahim F. Rehan, Ibrahim F. Rehan, František Zigo, Abdelfattah Z. M. Salem, Abdelfattah Z. M. Salem

“…IntroductionAs a low-cost, high-fibre biomass resource, Phragmites australis (reed) has significant potential for feed applications, particularly as a partial replacement for conventional roughage in ruminant diets.MethodsThis study investigated the effects of integrating Bacillus subtilis (B. subtilis BNCC109047) with homofermentative/ heterofermentative lactic acid bacteria (LAB) consortia on the fermentation and nutritional quality of Phragmites australis (reed) silage. …”
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Penyisihan Material Organik dan Nitrogen dengan Proses Aerasi Menggunakan Microbubble Generator (MBG) pada Instalasi Pengolahan Air Limbah (IPAL) Asrama by Hafasatya Maharani Putri, Sri Puji Saraswati, Johan Syafri Mahathir

“…The total energy consumption with intermittent aeration process with an average discharge of 537.84 l/day is 14.12 kWh/m3 and a cost of Rp. 20,414/m3 with the largest energy use being phosphate removal at 5.10 kWh/gPO4-P, then ammonia removal at 1.79 kWh/gNH3-N, TN removal at 1.95 kWh/gTN and COD removal at 0.45 kWh/gCOD.…”
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Global Protein Interactome Mapping in Rice Using Barcode‐Indexed PCR Coupled with HiFi Long‐Read Sequencing by Xixi Liu, Dandan Xia, Jinjin Luo, Mengyuan Li, Lijuan Chen, Yiting Chen, Jie Huang, Yanan Li, Huayu Xu, Yang Yuan, Yu Cheng, Zhiyong Li, Guanghao Li, Shiyi Wang, Xinyong Liu, Wanning Liu, Fengyong Zhang, Zhichao Liu, Xiaohong Tong, Yuxuan Hou, Yifeng Wang, Jiezheng Ying, Abdullaev Mirtemir Baxodir ugli, Mukhammadjon Arabboevich Ergashev, Sanqiang Zhang, Wenya Yuan, Dawei Xue, Jianwei Zhang, Jian Zhang

“…However, the rice interactome has barely been studied due to the lack of massive, reliable, and cost‐effective methodologies. Here, the development of a barcode‐indexed PCR coupled with HiFi long‐read sequencing pipeline (BIP‐seq) is reported for high throughput Protein Protein Interaction (PPI)identification. …”
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Selective neoadjuvant therapy of rectal cancer patients (SELREC): study protocol for a European randomised controlled, open, multicentre non-inferiority trial by Markus W Büchler, Lukas Baumann, Peter Schirmacher, Christoph W Michalski, Markus K Diener, Dirk Jäger, Pascal Probst, Mohammed Al-Saeedi, Martin Schneider, Alexis Ulrich, Constantin Schwab, Anja Sander, Christina Klose, Christoph Kahlert, Hans-Ulrich Kauczor, Jürgen Debus, Rosa Klotz, Solveig Tenckhoff, Cosima Engerer, Kristin Uzun-Lang, Caroline Wild, Bruno C Köhler

“…That treatment approach may show fewer adverse effects and be more cost-effective.Methods and analysis Selective neoadjuvant therapy of rectal cancer patients (SELREC) is a randomised controlled, parallel-group, open, multicentre, non-inferiority trial. …”
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