Singleplex real-time PCR and duplex PCR platform for the rapid detection of hypervirulent Klebsiella pneumoniae by Jiaqi Wang, Jiaqi Wang, Jiaqi Wang, Yishuai Wu, Yishuai Wu, Yishuai Wu, Liying Zhao, Liying Zhao, Liying Zhao, Liying Zhao, Mengran Xing, Mengran Xing, Mengran Xing, Mengran Xing, Yangyang Huang, Yangyang Huang, Yangyang Huang, Shengjie Peng, Shengjie Peng, Shengjie Peng, Chunyan Xu, Chunyan Xu, Chunyan Xu, Hong Yao, Hong Yao, Hong Yao, Chenglong Li, Chenglong Li, Chenglong Li, Xiang-Dang Du, Xiang-Dang Du, Xiang-Dang Du

“…To improve the efficiency and reduce the cost of diagnostic testing, SYBR Green I-based duplex PCR melting curve assays were developed with average melting temperatures of 85.0, 87.5, 76.0 and 78.5°C for iroB, iucA, peg-344 and plasmid-borne rmpA, respectively. …”
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Efficacy and safety of varenicline and bupropion, in combination and alone, for alcohol use disorder: a randomized, double-blind, placebo-controlled multicentre trialResearch in co... by Bo Söderpalm, Helga Lidö, Johan Franck, Anders Håkansson, Daniel Lindqvist, Markus Heilig, Joar Guterstam, Markus Samuelson, Barbro Askerup, Cecilia Wallmark-Nilsson, Andrea de Bejczy

“…Summary: Background: Alcohol use disorder (AUD) is associated with an enormous burden of disease and cost to society. The dopamine deficiency hypothesis posits that negative reinforcement generated by a low brain dopamine state drives ethanol intake. …”
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The optimal strategy for seasonal influenza vaccination to prevent high-intensity level of influenza epidemics in Zhejiang, China: an integrated transmission-dynamic and health-eco... by Mengsha Chen, Mengya Yang, Rui Yan, Zhengwei Liu, Can Chen, Rongrong Qu, Wenkai Zhou, Jiaxing Qi, Kexin Cao, Jiani Miao, Xiaoyue Wu, Jiaxin Chen, Qianqian Feng, HuiHui Zhang, Anqi Dai, Yi Yang, Jingtong Zhou, Qin Chen, Jimin Sun, Shigui Yang

“…Targeting the 0–14 years and 60 + years age groups proves to be the most cost-effective strategy under constrained coverage conditions. …”
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The Journey to Advanced Clinical Pharmacy Practice: Global Collaboration Will Accelerate the Pace by Paul W. Bush

“…PAI 2030 themes for practice change focus on optimizing care via pharmacist-provided comprehensive medication management, integrating the pharmacy enterprise for convenient and cost-effective care, advancing pharmacy technician roles, and adopting personalized, targeted therapies. [5] Globally, pharmacy practice is evolving to patient focused inter professional team-based care. …”
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Research on Thermodynamic Properties of the CO₂ Medium During Phase Transition Excited by Energetic Agent by SHEN Zhiqiang, XIA Jun, ZHANG Fanzhen, YANG Lijun

“…In order to find the best balance position between the acceptable computational cost and prediction accuracy of the zero-dimensional theoretical model, the following additional assumption were introduced. …”
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Investigating the Relationship between Financialization and Long-Term Use of Short-Term Debt by Seyed Reza Seyed Nezhad Fahim

“…Firms with a financial cost-to-total debt ratio exceeding the median are classified as facing resource acquisition restrictions and assigned a value of one; otherwise, they are assigned a value of zero. …”
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Regulatory effects of laser irradiation on greenhouse-grown strawberry productivity by LEI Kangqi, YANG Han, CHENG Feng, LEI Yumeng, LI Jianwei, QI Yetong, LI Zhitao, QI Xingjiang

“…Unlike traditional fill lights, semiconductor lasers can induce and promote plant growth, development, and yield increase in a low-intensity mode, providing a low-cost and high-efficiency “light fertilizer” for facility agriculture.…”
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International expert consensus on surgery for type 2 diabetes mellitus by Mohammad Kermansaravi, Islam Omar, Nicholas Finer, Carel Le Roux, Miguel A. Carbajo, David Sarwer, Luca Busetto, Jaime Ponce, Jennifer Logue, Helen M. Parretti, Mary O’Kane, Shahab Shahabi, Kamlesh Khunti, Alexandra I. Blakemore, Erik Stenberg, Sally Abbott, Aayed Alqahtani, Ali Aminian, Bassem Amr, Jose M. Balibrea, Rachel L. Batterham, Estuardo Behrens, Deepak L. Bhatt, Paul Chesworth, Pradeep Chowbey, Ken Clare, Manoel Galvao Neto, Yitka Graham, Ramen Goel, Wasim Hanif, Miguel F. Herrera, Kazunori Kasama, Radwan Kassir, Filip K. Knop, Shanu N. Kothari, Jon A. Kristinsson, Barbara McGowan, Andrew McKechnie, Karl Miller, Alex D. Miras, John Morton, Jane Ogden, Ralph Peterli, Jonathan H. Pinkney, Dimitri Pournaras, Sjaak Pouwels, Gerhard Prager, Paulina Salminen, Mireille J. Serlie, Asim Shabbir, Rishi Singhal, Shahrad Taheri, Abd A. Tahrani, Rudolf Weiner, Scott A. Shikora, Kamal Mahawar

“…Additionally, they agreed that metabolic surgery for T2DM is cost-effective, and MBS procedures for treating T2DM vary in their safety and efficacy. …”
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Ultrasound Phenotype-Based Approach to Treatment Choice in Osteoarthritis by Rositsa Karalilova, Velichka Popova, Konstantin Batalov, Dimitar Kolev, Lyatif Kodzhaahmed, Dimitrina Petrova-Stoyankova, Nikola Tepeliev, Tsvetelina Kostova, Lili Mekenyan, Zguro Batalov

“…It is one of the most common chronic, socially significant diseases, associated with a decline in the quality of life of patients and constantly increasing the cost of treatment. Clinical trial outcomes are largely inconclusive, and OA remains one of the few musculoskeletal diseases without an established disease-modifying therapy. …”
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Accuracy of tracer-based methane flux quantification: underlying impact of calibrating acetylene measurements by A. Shah, O. Laurent, P. Kumar, G. Broquet, L. Loigerot, T. Depelchin, M. Lozano, C. Yver Kwok, C. Philippon, C. Romand, E. Allegrini, M. Trombetti, P. Ciais

“…Acetylene is a commonly used methane tracer due to its availability, low cost and low atmospheric background. Acetylene mole fraction can be measured using infrared gas analysers such as the cavity ring-down spectroscopy Picarro G2203. …”
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Thermodielectric Properties of Polyurethane Composites with Aluminium Nitride and Wurtzite Boron Nitride Microfillers: Analysis Below and near Percolation Threshold by Alexey Gunya, Jozef Kúdelčík, Štefan Hardoň, Marián Janek

“…Thermal measurements were conducted using a newly designed, cost-effective thermal measurement setup. The setup utilised a transient heat pulse methodology with a heater and NTC thermistors, with a precision better than <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><mo>±</mo><mn>0.01</mn></mrow></semantics></math></inline-formula> W·<inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><msup><mi mathvariant="normal">m</mi><mrow><mo>−</mo><mn>1</mn></mrow></msup><mo>·</mo></mrow></semantics></math></inline-formula><inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><msup><mi mathvariant="normal">K</mi><mrow><mo>−</mo><mn>1</mn></mrow></msup></semantics></math></inline-formula>. …”
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Exploration of the Main Models and Optimization Paths for the Market-Oriented Operation of Urban Parks by Yanting LI, Qiaowan YANG

“…With cultural and digital assets as the core to activate low-cost and high-efficiency sustainable operations, diversified financing channels are explored. …”
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Effect of mixed fermentation with Lacticaseibacillus paracasei SMN-LBK and Limosilactobacillus reuteri LBK on the flavor precursor and flavor formation of Kazak cheese by Yuechenfei Shen, Jie Shen, Ning Kang, Yu Gao, Zhexin Fan, Zhifeng Fang, Jiancheng Wang, Baokun Li, Bo Yang

“…Previous research has demonstrated that Lacticaseibacillus paracasei notably enhances flavor stability with the cost of diminishing the inherent milk flavor in cheese, which may be rescued by the incorporation of Limosilactobacillus reuteri. …”
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An Explainable Multimodal Artificial Intelligence Model Integrating Histopathological Microenvironment and EHR Phenotypes for Germline Genetic Testing in Breast Cancer by Zijian Yang, Changyuan Guo, Jiayi Li, Yalun Li, Lei Zhong, Pengming Pu, Tongxuan Shang, Lin Cong, Yongxin Zhou, Guangdong Qiao, Ziqi Jia, Hengyi Xu, Heng Cao, Yansong Huang, Tianyi Liu, Jian Liang, Jiang Wu, Dongxu Ma, Yuchen Liu, Ruijie Zhou, Xiang Wang, Jianming Ying, Meng Zhou, Jiaqi Liu

“…By bridging digital pathology with clinical phenotypes, MAIGGT establishes a new paradigm for cost‐effective, scalable, and biologically interpretable prescreening of hereditary breast cancer, with the potential to significantly improve the accessibility of genetic testing in routine clinical practice.…”
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Progress in silicon-based reconfigurable and programmable all-optical signal processing chips by Jing Xu, Wenchan Dong, Qingzhong Huang, Yujia Zhang, Yuchen Yin, Zhenyu Zhao, Desheng Zeng, Xiaoyan Gao, Wentao Gu, Zihao Yang, Hanghang Li, Xinjie Han, Yong Geng, Kunpeng Zhai, Bei Chen, Xin Fu, Lei Lei, Xiaojun Wu, Jianji Dong, Yikai Su, Ming Li, Jianguo Liu, Ninghua Zhu, Xuhan Guo, Heng Zhou, Huashun Wen, Kun Qiu, Xinliang Zhang

“…In this paper, we review the recent progress in the project granted to develop silicon-based reconfigurable AOSP chips, which aims to combine the merits of AOSP and silicon photonics to solve the unsustainable cost and energy challenges in future communication and big data applications. …”
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When “Aging” meets “Intelligence”: smart health cognition and intentions of older adults in rural Western China by Xiao-Yu Li, Xiao-Yu Li, Xiao-Yu Li, Jun Li, Ning-Li Zhu, Ning-Li Zhu, Ning-Li Zhu, Lei Luo, Lei Luo, Lei Luo, Si-Yu Zhang, Si-Yu Zhang, Si-Yu Zhang, Wen-Wen Cheng, Wen-Wen Cheng, Wen-Wen Cheng, Jia-Xue Li, Jia-Xue Li, Jia-Xue Li, Chen Yu, Chen Yu, Chen Yu, Song-He Lu, Liang Zhu, Liang Zhu, Liang Zhu

“…Moreover, concerted efforts by governments, enterprises, and all sectors of society are essential to deliver more accessible and cost-effective health management and services to older adults. …”
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Challenges and Obstacles in the Development of Winter Sports Tourism in the Ski Slopes of Tehran by Siavash Imeni, Leyla Asadpour Shirazi

“…Ski resorts are relatively inaccessible for women in terms of time and cost, emphasizing physical challenge and danger over health and fitness, leading many women to feel that skiing does not align with their interests and lifestyle. …”
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A phase II randomized trial of individualized neoantigen peptide vaccine combined with unusual radiotherapy (iNATURE) in advanced solid tumors-GCOG0028 by Yan Zhang, Yan Zhang, Yan Zhang, Ye-Fan Hu, Ye-Fan Hu, Lingyu Ma, Lingyu Ma, Yifei Wu, Yifei Wu, Dandan Chao, Dandan Chao, Xian Chen, Xian Chen, Zhiyuan Xu, Zhiyuan Xu, Xiaoping Su, Xiaoping Su, Wei Dai, Wei Dai, Wei Dai, Jiandong Huang, Jiandong Huang, Jiandong Huang, Jiandong Huang, Pingfu Fu, Feng-Ming (Spring) Kong, Feng-Ming (Spring) Kong, Feng-Ming (Spring) Kong, Feng-Ming (Spring) Kong

“…This approach may reduce preparation time and cost, facilitating broader implementation of neoantigen-based immunotherapies. …”
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Insights from the trial innovation network’s initial consultation process by Paul A. Harris, Nan Kennedy, Consuelo H. Wilkins, Karen Lane, Gordon R. Bernard, Jonathan D. Casey, Daniel E. Ford, Salina P. Waddy, Ken L. Wiley, Terri L. Edwards, Nichol McBee, Dixie D. Thompson, Mary Stroud, Emily S. Serdoz, Sarah J. Nelson, Michelle Jones, Lindsay M. Eyzaguirre, Leslie R. Boone, Jessica Baird, Colleen E. Lawrence, Elizabeth Holthouse, Sarah K. Cook, Maeve Tischbein, Natalya Amrine, Tiffany Chen, Jodie Cohen, LaShondra Deyampert, Natalie A. Dilts, Delicia Burts, Amna Baig, Joseph G. Christodoulou, Mariela Rodriguez, Edgar R. Miller, James F. Casella, W. Andrew Mould, J. Michael Dean, Daniel K. Benjamin, Harry P. Selker, Marisha E. Palm, Lori Poole, Jeri S. Burr, Sara Hassani, Angeline Nanni, Meghan Hildreth, Daniel F. Hanley

“…The TIN offers a scientific consultation process, providing access to clinical trial and disease experts who provide input and recommendations throughout the trial’s duration, at no cost to investigators. This approach aims to improve trial design, accelerate implementation, foster interdisciplinary teamwork, and spur innovations that enhance multicenter trial quality and efficiency. …”
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[18F]FET PET-Guided management of pseudoprogression in glioblastoma (FET POPPING): the study protocol for a diagnostic randomized clinical trial by V.J. Ruijters, T.J. Snijders, J.A.J. van der Pol, E.M. van de Giessen, J.M. Niers, M.P.G. Broen, M.M. Anten, S.E.M. Veldhuijzen van Zanten, M. Geurts, A.I.J. Arens, D.J.H.A. Henssen, J.M. Gijtenbeek, M. van der Meulen, A.E.J. Sijben, E. Ghariq, M.J. Vos, J. Tim, I.B. Bosma, G.N. Stormezand, G.W.J. Frederix, J.W. Dankbaar, P.A. Robe, J.J.C. Verhoeff, F.Y.F.L. de Vos, M.G.E.H. Lam, R.M.T. ten Ham, N. Tolboom

“…Still, this diagnostic tool is not used frequently in The Netherlands due to costs, logistics and uncertainty about clinical benefit. …”
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