Droplet-based high-throughput single microbe RNA sequencing by smRandom-seq by Ziye Xu, Yuting Wang, Kuanwei Sheng, Raoul Rosenthal, Nan Liu, Xiaoting Hua, Tianyu Zhang, Jiaye Chen, Mengdi Song, Yuexiao Lv, Shunji Zhang, Yingjuan Huang, Zhaolun Wang, Ting Cao, Yifei Shen, Yan Jiang, Yunsong Yu, Yu Chen, Guoji Guo, Peng Yin, David A. Weitz, Yongcheng Wang

“…Here, we report a droplet-based high-throughput single-microbe RNA-seq assay (smRandom-seq), using random primers for in situ cDNA generation, droplets for single-microbe barcoding, and CRISPR-based rRNA depletion for mRNA enrichment. smRandom-seq showed a high species specificity (99%), a minor doublet rate (1.6%), a reduced rRNA percentage (32%), and a sensitive gene detection (a median of ~1000 genes per single E. coli). …”
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Effect of storage temperature on freshness of silver carp (Hypophthalmichthys molitrix) by YAO Yan-jia, ZHANG Jin-jie, GU Wei-gang, JI Rong, HU Ya-qin

“…Moreover, the results show that frozen storage is an effective method to slow down the rate of freshness decrease of silver carps.…”
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Internet of things in healthcare monitoring to enhance acquisition performance of respiratory disorder sensors by Leonardo Juan Ramírez López, Arturo Rodriguez Garcia, Gabriel Puerta Aponte

“…The proposed model is validated on the measurement and monitoring of oxygen saturation, heart rate, and body temperature in patients with respiratory disorders. …”
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Exploring heterogeneity of perimenopause with application of multivariable analysis approaches by Zhongyu Huang, Huazhi You, Lijuan Li, Shuang Wang, Zipan Lyu, Xiaoqin Zeng, Changyan Zhu, Minqing Li, Han Yan, Yaojuan He

“…The assessing framework was developed over the initial item pool as integration of 4 scales including Insomnia severity index, Modified Kupperman index, Self-rating anxiety scale, and Self-rating depression scale. …”
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An interpretable machine learning approach for predicting and grading hip osteoarthritis using gait analysis by Qing Yang, Xinyu Ji, Yuyan Zhang, Shaoyi Du, Bing Ji, Wei Zeng

“…Purpose The purpose of this study is to evaluate the efficacy of lower extremity kinematic gait data for detecting and rating the severity of unilateral hip OA using machine learning algorithms. …”
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Source apportionment of potentially toxic elements in soils from an urbanising region: Insights from multivariate analysis in Singida, Tanzania by Raymond Webrah Kazapoe, Benatus Norbert Mvile, John Desderius Kalimenze, Daniel Kwayisi, Samuel Dzidefo Sagoe, Kwabina Ibrahim, Obed Fiifi Fynn

“…Cluster 2 is shown to be more prominent in the Northern part of the area such as Sekenke, Shelui, Lambi, Mtinko and New Kiomboi. Due to the growing rate of urbanisation, these areas have become relatively populous and have a high level of anthropogenic activities, such as gold mining, sunflower oil milling and agricultural activities which have been shown in the study to influence the spatial patterns of PTEs in the area. …”
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Spatial analysis and prediction of psittacosis in Zhejiang Province, China, 2019–2024 by Zheyuan Ding, Haocheng Wu, Chen Wu, Kui Liu, Qinbao Lu, Xinyi Wang, Tianying Fu, Junjie Li, Ke Yang, Queping Song, Junfen Lin

“…Spatial epidemiological methods, including spatiotemporal distribution mapping, spatial autocorrelation analysis, and Kriging interpolation, were employed to identify disease hotspots and predict risk areas.ResultsDuring the study period, 315 psittacosis cases were reported, with an annual average incidence rate of 0.0914 per 100,000 population, showing a significant increasing trend. …”
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Nettle (Urtica Dioica L.) Modulates Angiogenesis by Targeting the PI3K/AKT/eNOS Pathway in Prostate Cancer by Abduladheem AL-Attabi, Mohammed Sabah Mohammed, Noor Ali Yazi AL-Khazali, Sami Awad Alkubaisy, Muna S. Merza, Mohanad Ali Abdulhadi, Yasser Fakri Mustafa

“…Moreover, the apoptosis rate of DU-145 cells was enhanced compared with the control group.Conclusion: The antitumoral activity of UDE was prominent in its persuasive anti-angiogenic potential, as UDE contributed to a striking diminish in PI3K/AKT/eNOS pathway in PCa and diminished the VEGF expression.…”
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Beyond Bleeding: An Analysis of Presenting Symptoms Among Black Patients with Endometrial Cancer by Maya E. Gross, Mindy Pike, Julianna Alson, Patrice Williams, Mollie E. Wood, Erica Marsh, Erin Carey, Til Stürmer, Ronit Katz, Whitney R. Robinson, Kemi M. Doll

“…Objectives: Black patients have the highest mortality rate from endometrial cancer (EC), and yet remain underrepresented in EC research. …”
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Lymphatic system is the mainstream for breast cancer dissemination and metastasis revealed by single-cell lineage tracing by Kai Miao, Aiping Zhang, Xiaodan Yang, Yipeng Zhang, Anqi Lin, Lijian Wang, Xin Zhang, Heng Sun, Jun Xu, Jingyao Zhang, Yuzhao Feng, Fangyuan Shao, Sen Guo, Zhihui Weng, Peng Luo, Dong Wang, Shuai Gao, Xiao-Yang Zhao, Xiaoling Xu, Chu-Xia Deng

“…In this study, we applied a barcoding system based single-cell lineage tracing approach to study the metastasis rate and route of breast cancer cells and their interactions with the tumor microenvironment (TME) during metastasis. …”
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Study on main controlling factors of CO2 huff-n-puff for enhanced oil recovery and storage in shale oil reservoirs by CHEN Jun, WANG Haimei, CHEN Xi, TANG Yong, TANG Liangrui, SI Rong, WANG Huijun, HUANG Xianzhu, LENG Bing

“…Considering both oil recovery and storage, the optimal injection strategy for a single well included: initiating when daily oil production declined to just above 8 m3, injecting 15 000-24 000 tons of CO₂ at a rate of 500-900 t/d, shut-in duration of 30-50 days, and conducting 2-3 huff-n-puff cycles. …”
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Physics-based modeling and mechanism of polycrystalline diamond tool wear in milling of 70 vol% Si/Al composite by Lianjia Xin, Guolong Zhao, Zhiwen Nian, Haotian Yang, Liang Li, Ning He

“…The optimal machining parameters of 120 m·min ^–1 cutting speed ( v _c ) and 0.04 mm·z ^–1 feed rate ( f _z ), result in tool life of 33 min and surface roughness ( S _a ) of 2.2 μm. …”
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Developing and Validating Species Distribution Models for Wetland Plants Across Europe by Ojaswi Sumbh, Marjon Hellegers, Valerio Barbarossa, Renata Ćušterevska, Borja Jiménez‐Alfaro, Łukasz Kozub, Francesca Napoleone, Zvjezdana Stančić, Aafke M. Schipper

“…We concluded that our SDMs are suitable for predicting broad‐scale patterns of wetland plant species distributions as governed by climatic conditions. …”
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Effect of IAA on coumarins synthesis in Angelica dahurica under soil compactness stress by Xinglong Zhu, Xiaoyan Chen, Chengfeng Huang, Yongfeng Zhou, Tao Zhou, Qinghua Wu, Jin Pei

“…It was found that both too low and too high soil compactness resulted in low biomass accumulation of A. dahurica, but the latter showed a higher rate of coumarins accumulation. IAA was positively correlated (p < 0.1) with various morphological parameters and total coumarins content, suggesting that IAA may be associated with coumarins synthesis. …”
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Reinforcement Learning With Deep Features: A Dynamic Approach for Intrusion Detection in IoT Networks by Mohamad Khayat, Ezedin Barka, Mohamed Adel Serhani, Farag Sallabi, Khaled Shuaib, Heba M. Khater

“…Then, domain-specific features related to network traffic patterns, including packet size distribution, packet count, and packet rate, were extracted. …”
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Bibliometrics Analysis and Knowledge Mapping of Fragment-Based Drug Design Research: Trends from 2015 to 2024 by He C, Zhang J, Zhang R, Liu L, Luo J, Zhang W, Fang B

“…Bibliometric analysis and knowledge mapping were conducted using the RStudio’s bibliometrix-biblioshiny package, CiteSpace, and VOSviewer software, assessing multiple dimensions such as journal co-occurrence, keyword density, institutional collaboration, and citation patterns.Results: The research output in FBDD revealed fluctuating growth, with an average annual growth rate of 1.42%. …”
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Vacation and back-to-school effect on influenza transmission among school-age children in Guangzhou, China: an ecological study from 2010 to 2023 by Di Wu, Mengmeng Ma, Yanhui Liu, Zhitao Chen, Meiqi Zhang, Dedong Wang, Pengzhe Qin, Lei Luo

“…Objective To examine whether influenza transmission risk decreased during summer and winter vacations and increased after vacations and statutory holiday.Design An ecological study.Setting Guangzhou, Guangdong Province of China.Participants A total of 458 343 influenza cases in school-aged children were included, including 160 067 (35.05%) in kindergarten, 223 817 (48.83%) in primary school, 50 053 (10.92%) in middle school and 23 801 (5.19%) in high school.Data and methods Data on influenza cases were obtained from the Notifiable Infectious Disease Surveillance System operated by Guangzhou Centre for Disease Control and Prevention. Influenza incidence rate ratio (IRR) by negative binomial regression model was used to analyse the influenza risk of school-age children during/after vacation to before/during breaks.Results In all four groups, there was significantly lower incidence of influenza during winter and summer vacations compared with the 4 weeks preceding vacations. …”
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Association of Korean Medicine and polypharmacy with fall risk and mortality in older adults with stroke by Ye-Seul Lee, Bo-Hyoung Jang, Jin Pyeong Jeon, Han-Gyul Lee, Seungwon Kwon, Woo-Sang Jung

“…KM users with polypharmacy had a higher rate of falls but lower all-cause mortality than non-users. …”
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A Systematic Review and Meta-analysis of Most Endoparasites and Ectoparasites during Past Decade in Iraq by Karwan S. Najm Al-Marjan, Hawri H. Mohammed Jabbary, Liza J. Yousif, Fouad H. Kamil, Najat J. Ahmed

“…During the reviewed period, high prevalence of infection was recorded in several Iraqi provenances including, E. histolytica from Kirkuk (79.49%), T. gondii from in AL-Amaara (18.07%), G. lamblia and T. vaginalis from AL-Basrah (11.05%, 55.4%, respectively), and the head lice, P. humanus capiotis from Koya District at Erbil provenance (41.14%). Variation in the rate of infection/infestation tells us that there is some instability among study area including, instability of the security and military situation, economic instability, population density instability. …”
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Reducing Plasmodium falciparum malaria transmission in Africa: a model-based evaluation of intervention strategies. by Jamie T Griffin, T Deirdre Hollingsworth, Lucy C Okell, Thomas S Churcher, Michael White, Wes Hinsley, Teun Bousema, Chris J Drakeley, Neil M Ferguson, María-Gloria Basáñez, Azra C Ghani

“…We then explored the impact on transmission of continued roll-out of LLINs, additional rounds of indoor residual spraying (IRS), mass screening and treatment (MSAT), and a future RTS,S/AS01 vaccine in six representative settings with varying transmission intensity (as summarized by the annual entomological inoculation rate, EIR: 1 setting with low, 3 with moderate, and 2 with high EIRs), vector-species combinations, and patterns of seasonality. …”
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