Droplet-based high-throughput single microbe RNA sequencing by smRandom-seq by Ziye Xu, Yuting Wang, Kuanwei Sheng, Raoul Rosenthal, Nan Liu, Xiaoting Hua, Tianyu Zhang, Jiaye Chen, Mengdi Song, Yuexiao Lv, Shunji Zhang, Yingjuan Huang, Zhaolun Wang, Ting Cao, Yifei Shen, Yan Jiang, Yunsong Yu, Yu Chen, Guoji Guo, Peng Yin, David A. Weitz, Yongcheng Wang

“…Here, we report a droplet-based high-throughput single-microbe RNA-seq assay (smRandom-seq), using random primers for in situ cDNA generation, droplets for single-microbe barcoding, and CRISPR-based rRNA depletion for mRNA enrichment. smRandom-seq showed a high species specificity (99%), a minor doublet rate (1.6%), a reduced rRNA percentage (32%), and a sensitive gene detection (a median of ~1000 genes per single E. coli). …”
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Ecological drivers of nesting behavior in a subtropical city: An observational study on spotted doves by Yao Sheng, Mengjie Lu, Junpeng Bai, Xiaobin Xie, Long Ma, Wanyou Li, Zhen Zhang, Fang Ming, Xueli Zhang, Ziwei Zhang, Zhifeng Xu, Yuqing Han, Bicai Guan, Luzhang Ruan

“…The results revealed that the nest reuse rate was 38.08% (n = 115). Nests closer to trunk, with lower nest position and higher large‐scale urbanization score tended to have higher reuse rate. …”
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Metabolomics Biomarkers in Prediction of Sudden Infant Death Syndrome: The Role of Short Chain Fatty Acids by Maria Aslam, Omer Riaz, Jawaria Aslam, Dost Muhammad Khan, Mustafa Hameed, Muhammad Suleman, Rizwan Shahid, Turke Althobaiti, Naeem Ramzan

“…Leveraging machine learning (ML) presents a promising avenue for unraveling the intricate profiles of SCFA and decoding patterns indicative of heightened SIDS risk. Ensuring interpretability in healthcare is essential for building trust and developing effective prevention strategies. …”
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Nailfold videocapillaroscopy in patients with deficiency of adenosine deaminase 2 (DADA2): a case-control study by Pietro Francesco Bica, Caterina Matucci-Cerinic, Elvis Hysa, Andrea Cere, Silvia Rosina, Stefano Volpi, Roberta Caorsi, Alberto Sulli, Sabrina Paolino, Vanessa Smith, Maurizio Cutolo, Marco Gattorno

“…The capillary density was normal and no scleroderma pattern was found. Between DADA2, RP patients and HC, no significant differences in the rate of each microvascular finding were detected (p values NS). …”
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Altered dynamic functional network connectivity in rheumatoid arthritis associated with peripheral inflammation and neuropsychiatric disorders by Lei Xie, Yanmin Zheng, Zhiduo Hou, Shuhua Ma, Zikai Huang, Jianhua Peng, Shuxin Huang, Ruiwei Guo, Jinzhuang Huang, Zhirong Lin, Zelin Zhuang, Jingjing Yin

“…The Spearman correlation was calculated between aberrant DFC alterations, Montreal Cognitive Assessment (MoCA), Hospital Anxiety and Depression Scale (HAD), C reactive protein (CRP) and erythrocyte sedimentation rate (ESR). Diagnostic efficacy of indicators was assessed using receiver operating characteristic analysis (ROC).Results Three dynamic functional states were identified. …”
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T-cell repertoire correlates with cytokine imbalance in multiple sclerosis patients by Lisa Weidner, Lisa Weidner, Rodolphe Poupardin, Tobias Zrzavy, Sandra Laner-Plamberger, Georg Gratz, Tanja Eichhorn, Viktoria Weber, Paulus S. Rommer, Christof Jungbauer, Christof Jungbauer, Dirk Strunk, Dirk Strunk

“…TCR sequencing revealed more productive rearrangements in CSF of MS and a significantly higher shared clone recovery rate in blood. We found significant associations involving 492 unique sequences and 34 cytokines in blood. …”
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Plasma state monitoring and disruption characterization using multimodal VAEs by Yoeri Poels, Alessandro Pau, Christian Donner, Giulio Romanelli, Olivier Sauter, Cristina Venturini, Vlado Menkovski, the TCV Team, the WPTE Team

“…Subsequently, we can identify continuous indicators for the disruption rate and the disruptivity based on statistical properties of measurement data. …”
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Multi-Objective Optimization of Off-Grid E-Bikes Charging Stations Powered by PhotoVoltaics by Fabio Corti, Gabriele Maria Lozito, Davide Astolfi, Salvatore Dello Iacono, Antony Vasile, Marco Pasetti, Alberto Reatti, Alessandra Flammini

“…A Genetic Algorithm is employed to determine the most appropriate rated power of the installed PhotoVoltaic (PV) systems and of the energy storage, by incorporating statistical methods to estimate the daily number of e-bikes requiring charging, hence making the optimization process more reflective of actual usage patterns. …”
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Behavioral Responses of Migratory Fish to Environmental Cues: Evidence from the Heishui River by Jiawei Xu, Yilin Jiao, Shan-e-hyder Soomro, Xiaozhang Hu, Dongqing Li, Jianping Wang, Bingjun Liu, Chenyu Lin, Senfan Ke, Yujiao Wu, Xiaotao Shi

“…Fish exhibited distinct diel migration patterns; upstream movements were largely nocturnal, whereas downstream migration mainly occurred during daylight. …”
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Apnea detection using wrist actigraphy in patients with heterogeneous sleep disorders by Xiaoman Xing, Sizhi Ai, Jihui Zhang, Rui Huang, Yaping Liu, Dongming Quan, Jiacheng Ma, Guoli Wu, Jiangen Xu, Yuan Zhang, Hongliang Feng, Wen-fei Dong

“…Wrist actigraphy is a widely-used and energy-efficient tool for respiratory rate estimation. The main challenge in OSA pattern recognition is handling various disturbances in real-world applications. …”
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Dual smart sensor data-based deep learning network for premature infant hypoglycemia detection by Muhammad Shafiq, J. Kavitha, Dhruva R. Rinku, N. K. Senthil Kumar, Kamal Poon, Amar Y. Jaffar, V. Saravanan

“…Based on features, the sorted-out data gets evaluated through a GRU-LSTM (Gated Recurrent Unit - Long Short-Term Memory) network to identify the state of the infant as usual and suggestive of hypoglycemia—blood glucose below 70 mg/dL, pale complexion, profuse perspiration. …”
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Transfer learning for securing electric vehicle charging infrastructure from cyber-physical attacks by Ahmad Almadhor, Shtwai Alsubai, Imen Bouazzi, Vincent Karovic, Monika Davidekova, Abdullah Al Hejaili, Gabriel Avelino Sampedro

“…The study also provides a comparison with different DL models such as Long Short-Term Memory (LSTM), Recurrent Neural Networks (RNN), Long Short-Term Memory-Recurrent Neural Networks (LSTM-RNN), and Gated Recurrent Unit (GRU). The CICEVSE2024 (EVSE-A and EVSE-B) datasets are used to assess the framework, where one dataset is used to train and store weights, and the second is used to evaluate the learned patterns using transfer learning. …”
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Lymphatic system is the mainstream for breast cancer dissemination and metastasis revealed by single-cell lineage tracing by Kai Miao, Aiping Zhang, Xiaodan Yang, Yipeng Zhang, Anqi Lin, Lijian Wang, Xin Zhang, Heng Sun, Jun Xu, Jingyao Zhang, Yuzhao Feng, Fangyuan Shao, Sen Guo, Zhihui Weng, Peng Luo, Dong Wang, Shuai Gao, Xiao-Yang Zhao, Xiaoling Xu, Chu-Xia Deng

“…In this study, we applied a barcoding system based single-cell lineage tracing approach to study the metastasis rate and route of breast cancer cells and their interactions with the tumor microenvironment (TME) during metastasis. …”
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An Adaptive Convolutional Neural Network With Spatio-Temporal Attention and Dynamic Pathways (ACNN-STADP) for Robust EEG-Based Motor Imagery Classification by Aaqib Raza, Mohd Zuki Yusoff

“…The proposed model integrates a Dynamic Pathway Convolution Network (DPCN) for adaptive feature extraction, incorporating a Dynamic Gating Controller (DGC) and Dynamic Adaptive Spatio-Temporal (DAST) blocks to efficiently capture multi-scale spatial and temporal dependencies. …”
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Spatial Heterogeneity of Bike-Sharing–Conventional Bus Intermodal Trip Distribution by Yang Chenyang, Tang Wenyun, Ma Jianxiao, Yin Chaoying, Wang Hanbin

“…The results indicated that the density of bike-sharing trips near bus stops followed a consistent pattern. The density decreases with increasing distance from the stop, and the rate of decline exhibits a "U-shaped" pattern. …”
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Systemic hormonal contraception and risk of venous thromboembolism by Oskari Heikinheimo, Elena Toffol, Timo Partonen, Anna But, Antti Latvala, Jari Haukka

“…This study aimed to assess the associations between the patterns of use of different systemic hormonal contraceptives and the risk of venous thromboembolism during 2017–2019. …”
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Optimizing fertilizer application and straw return to fields to minimize nitrogen and phosphorus runoff losses in double-rice cropping systems by Ying Zhang, Jian Zhu, Changjun Li, Hua Peng, Ji Liu, Yue Luo, Min Song, Yanjiao Dai, Kai Deng, Xionghui Ji

“…Unknown of long-term patterns of losses and mechanisms driving runoff N and P in double-rice cropping systems limited the development of effective mitigation strategies. …”
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Clinical benefit of tafasitamab plus lenalidomide in relapsed or refractory diffuse large Bcell lymphoma: real-world data from the EarlyMIND study by Gabriel Brisou, Jérôme Paillassa, Sophie Bernard, Olivier Tournilhac, Luc-Matthieu Fornecker, Frédéric Maloisel, Lauris Gastaud, Eric Durot, Adrian Tempescul, Thorsten Braun, Vadim Ivanov, Brieuc Cherel, Caroline Serrier, Jeremy Delage, Abderrazak El Yamani, Baptiste Delapierre, Laure Lebras, Elsa Lestang, Camille Villesuzanne, Philippe Agape, Guillaume Escure, Ludovic Fouillet, Christophe Nicol, Gaëlle Olivier, Anthony Levy, Anne-Sophie Le Bris, Ophélie Cassuto, Laura Jacquemelle, Corinne Haioun, Charles Herbaux

“…Approval was based on the L-MIND study, which demonstrated a 57.5% overall response rate (ORR), 41.3% complete response (CR) rate, 12.1 months median progression-free survival (PFS), and 33.5 months median overall survival (OS) at five years. …”
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Intraspecific and Intracolonial Variation in the Profile of Venom Alkaloids and Cuticular Hydrocarbons of the Fire Ant Solenopsis saevissima Smith (Hymenoptera: Formicidae) by Eduardo Gonçalves Paterson Fox, Adriana Pianaro, Daniel Russ Solis, Jacques Hubert Charles Delabie, Bruno Cunha Vairo, Ednildo de Alcântara Machado, Odair Correa Bueno

“…Some fire ant species are top-rated pests spreading throughout the world by trade vessels. …”
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Site of Service Changes Have Resulted in Increased Opioid Prescriptions for Primary Total Hip and Knee Patients in Michigan, a Michigan Arthroplasty Registry Collaborative Quality... by Harjot Uppal, MD, David Markel, MD, Giresse Melone, MD, Simarjeet Puri, MD, Lisheng Chen, PhD, Tae Kim, MHSA, Brian Hallstrom, MD, Richard Hughes, PhD, Elizabeth Dailey, MD

“…Background: The Michigan Arthroplasty Registry Collaborative Quality Initiative (MARCQI) successfully changed opioid prescribing patterns by instituting guidelines for total hip (THAs) and knee arthroplasties (TKAs). …”
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