Recent advances in self‐immobilizing fluorescent probes for in vivo imaging by Yong Zhang, Xuemei Lv, Yafu Wang, Xueqian Chen, Jinchao Zhang, Dongdong Su

“…The utilization of versatile fluorescent probes in fluorescence imaging offers a powerful tool for in vivo imaging of biomarkers closely associated with pathological conditions. …”
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Aptamer-based NIR II imaging for breast cancer surgical resection by Lei Niu, Kang-Liang Lou, Zhi Zhu, Wei-Zhi Liu, Wen-Liang Gao, Gu-Yue Hu, Jun-Xue Gao, Guo-Jun Zhang, Wen-He Huang

“…We develop an aptamer-conjugated NIR-II probe for intraoperative fluorescence imaging. Peglated indocyanine green (PEG-ICG) was conjugated with an aptamer SYL3C binding to EpCAM to synthesize the probe SYL3C-ICG. …”
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INTRAOPERATIVE DETECTION OF THE SENTINEL LYMPH NODES IN LUNG CANCER by A. L. Akopov, G. V. Papayan, I. V. Chistyakov

“…The authors present the results of an application of dye techniques, radioactive preparation and fluorescence imaging for sentinel lymph node detection. Advantages and disadvantages of the methods are shown in the article. …”
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The Benthic Underwater Microscope imaging PAM (BUMP): A non‐invasive tool for in situ assessment of microstructure and photosynthetic efficiency by Or Ben‐Zvi, Paul Roberts, Devin Ratelle, Joseph Snider, Pichaya Lertvilai, Daniel Wangpraseurt, Dimitri D. Deheyn, Jennifer E. Smith, Jules S. Jaffe

“…To achieve this goal, we designed, fabricated, and tested an underwater microscope that incorporates standard colour, epifluorescence, and variable chlorophyll a fluorescence imaging with nearly micron spatial resolution that resolves the structure and photosynthetic efficiency of benthic organisms. …”
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Application of aggregation-induced emission (AIE) in the urinary system disease by Xiangqian Cao, Yilin Yan, Xinyi Zhu, Zhengnan Huang, Chenkai Yang, Weiguang Zhao, Haoyuan Wang, Xiaodong Zhu, Bing Shen

“…This review systematically summarizes the applications of AIE materials in urinary cancers, such as bladder cancer, renal cancer, prostate cancer, and upper urinary tract cancer, including fluorescence imaging, photodynamic therapy, and drug delivery techniques. …”
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Biodistribution of nanoplastics in mice: advancing analytical techniques using metal-doped plastics by Theresa Staufer, Verena Kopatz, Alice Pradel, Tess Brodie, Robert Kuhrwahl, Deborah Stroka, Julia Wallner, Lukas Kenner, Verena Pichler, Florian Grüner, Denise M. Mitrano

“…To address this, we propose a workflow for NPs assessment in biological samples combining three complimentary methods, namely inductively coupled plasma mass spectrometry (ICP-MS), X-ray fluorescence imaging (XFI), and imaging mass cytometry (IMC) to detect palladium-doped NPs (Pd-NPs). …”
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Self-driving microscopy detects the onset of protein aggregation and enables intelligent Brillouin imaging by Khalid A. Ibrahim, Camille Cathala, Carlo Bevilacqua, Lely Feletti, Robert Prevedel, Hilal A. Lashuel, Aleksandra Radenovic

“…To overcome these limitations, we developed a self-driving microscope that uses deep learning to predict the onset of aggregation from a single fluorescence image of soluble protein, achieving 91% accuracy. …”
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The first experience of application of ICG-fluorescence for detection of segmental plane formation in thoracoscopic segmentectomy by V. G. Pishchik, A. I. Kovalenko, E. I. Zinchenko, A. D. Obornev

“…The implementation of this method significantly facilitates in thoracoscopic segmentectomy. The NIR fluorescence imaging systems allow clinicians to work with lower doses of ICG.…”
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Feasibility, Safety, and Early Outcomes of Image‐Guided Segmentectomy Using Near‐Infrared Fluorescence Dye for Tumor Visualization and Margin Identification: A Collaborative Effort... by Ching Feng Wu, Kuei An Chen, Ming Ju Hsieh, Yu Fu Wu, Tzu Yi Yang, Ching Yang Wu

“…Conclusion Overall, I‐segmentectomy with dual ICG fluorescence imaging is a feasible, safe, and effective method for ensuring adequate resection margins in difficult‐to‐discern lung lesions. …”
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Chemical-Free Rapid Lysis of Blood Cells in a Microfluidic Device Utilizing Ion Concentration Polarization by Suhyeon Kim, Seungbin Yoon, Hyoryung Nam, Hyeonsu Woo, Woonjae Choi, Geon Hwee Kim, Geunbae Lim

“…Here, we present a microfluidic device utilizing ion concentration polarization (ICP) for rapid blood cell lysis at 75 V. Fluorescence imaging confirmed the formation of an ion depletion region near the Nafion^® nanochannel membrane, where the electric field was concentrated across the entire microchannel width. …”
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Living photosynthetic micro/nano-platforms: Engineering unicellular algae for biomedical applications by Jian Wang, Haozhe Li, Qiangfei Su, Jingwei Liu, Kang Li, Zheng Wang, Lin Wang

“…As autotrophic biosystems, unicellular algae uniquely possess multi-functions, including oxygen generation, dynamic motility, fluorescence imaging, and programmable biosynthesis. Their photosynthetic systems not only generate therapeutic oxygen/hydrogen gradients but also facilitate chlorophyll-mediated therapeutics through inherent fluorescence and photodynamic effects. …”
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Time-deterministic cryo-optical microscopy by Kosuke Tsuji, Masahito Yamanaka, Yasuaki Kumamoto, Shoko Tamura, Wakana Miyamura, Toshiki Kubo, Kenta Mizushima, Kakeru Kono, Hanae Hirano, Momoko Shiozaki, Xiaowei Zhao, Heqi Xi, Kazunori Sugiura, Shun-ichi Fukushima, Takumi Kunimoto, Yoshino Tanabe, Kentaro Nishida, Kentaro Mochizuki, Yoshinori Harada, Nicholas I. Smith, Rainer Heintzmann, Zhiheng Yu, Meng C. Wang, Takeharu Nagai, Hideo Tanaka, Katsumasa Fujita

“…Enhancing the signal-to-noise ratio (SNR) in fluorescence imaging improves the quantification accuracy and spatial resolution; however, achieving high SNR at fast image acquisition rates, which is often required to observe cellular dynamics, still remains a challenge. …”
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Open educational resources for distributed hands-on teaching in molecular biology. by Ariel Cerda, Alejandro Aravena, Valentina Zapata, Anibal Arce, Wladimir Araya, Domingo Gallardo, Javiera Aviles, Francisco Quero, Isaac Nuñez, Tamara Matute, Felipe Navarro, Valentina Ferrando, Marta Blanco, Sebastian Velozo, Sebastian Rodriguez, Sebastian Aguilera, Francisco Chateau, Ruby Olivares-Donoso, Jennifer C Molloy, Guy Aidelberg, Ariel B Lindner, Fernando Castro, Pablo Cremades, Cesar Ramirez-Sarmiento, Fernan Federici

“…In this work, we describe Open Educational Resources (OER) composed of locally produced biological reagents, open source hardware and free software to teach fundamental techniques in biotechnology such as LAMP DNA amplification, RT-PCR RNA detection, enzyme kinetics and fluorescence imaging. The use of locally produced reagents and devices reduces costs by up to one order of magnitude. …”
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Smart molecular probes with controllable photophysical property for smart medicine by Xiaoying Kang, Zekun Du, Shuxuan Yang, Mengyun Liang, Qian Liu, Ji Qi

“…Phototheranostics such as fluorescence imaging (FLI), photoacoustic imaging (PAI), photodynamic therapy (PDT), and photothermal therapy (PTT) have attracted considerable attention in recent years, which mainly employ different excited‐state energy dissipation pathways of a chromophore. …”
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Indocyanine green fluorescence navigation for hepatocellular carcinoma with bile duct tumor thrombus: a case report by Masaru Matsumura, Yasuji Seyama, Hiroyuki Ishida, Satoshi Nemoto, Keigo Tani, Jun Imamura

“…After transection of the liver parenchyma, the right liver was connected with only the right hepatic duct. ICG fluorescence imaging visualized the tip of BDTT in the bile duct with clear contrast; the proximal side (hepatic side) of the right hepatic duct showed stronger fluorescence than the distal side (duodenal side). …”
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CaCO3-encircled hollow CuS nanovehicles to suppress cervical cancer through enhanced calcium overload-triggered mitochondria damage by Pengfei Wang, Xichen Sun, Liuyan Tang, Ningning Li, Qing Wang, Bicheng Gan, Yuezhou Zhang

“…Additionally, fluorescence imaging reveals the distribution of these nanovehicles in vivo, indicating their effective accumulation at the tumor site. …”
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Feasibility Assessment of an Optically Powered Digital Retinal Prosthesis Architecture for Retinal Ganglion Cell Stimulation by William Lemaire, Maher Benhouria, Konin Koua, Wei Tong, Gabriel Martin-Hardy, Melanie Stamp, Kumaravelu Ganesan, Louis-Philippe Gauthier, Marwan Besrour, Arman Ahnood, David John Garrett, Sebastien Roy, Michael R. Ibbotson, Steven Prawer, Rejean Fontaine

“…To demonstrate the capacity to elicit a neural response with this approach while complying with the optical irradiance limit at the pupil, fluorescence imaging with a calcium indicator is used on a degenerate rat retina. …”
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The use of fluorescence spectroscopy in combination with fluorescence vizualization for cystoscopy by А. А. Teplov, E. V. Filonenko, B. Ya. Alekseev, R. V. Ulianov, Yu. Yu. Andreeva

“…Local fluorescence spectroscopy combined with visual assessment of fluorescence image allowed to increase specificity of cystoscopy from 70% to 85%, total accuracy – from 80% to 86%, with improvement of predictive value for positive result from 0,67 to 0,94.…”
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Learning curve in robotic rectal cancer surgery: A national two-centre study by Anislav Ventsislavov Gabarski, Paulina T. Vladova, Martin P. Karamanliev, Nikolai Ramadanov, Aleksandar K. Zlatarov, Turgay T. Kalinov

“…The patients were divided into two groups: group I – the first 28 patients without indocyanine green (ICG) use and group II – the next 17 patients with ICG fluorescence imaging to assess bowel perfusion. Correlations between patient characteristics, operation duration, conversions, hospitalisation duration, complications, bleeding, reoperation, type of operation and ICG usage were assessed using multivariate analysis. …”
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Cascade-penetrating domino-ferroptosis nano inducer synergizes with sonodynamic therapy for anaplastic thyroid cancer by Peng Dong, Yun-Bo Chi, Deng-Ke Teng, Yuan-Qiang Lin, Ling-Yu Zhu, He-Qun Li, Jia-Yu Yang, Jia-Rui Du, Zong-Tao Zhang, Hai-Tao Ran, Guo-Qing Sui, Hui Wang, Qi-Meihui Wang

“…In terms of diagnosis, this nanoplatform combines multimodal (ultrasound, photoacoustic, magnetic resonance and fluorescence) imaging to improve the diagnostic performance of anaplastic thyroid cancer (ATC) and provide a visualization strategy for early diagnosis. …”
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